FastQCFastQC Report
Tue 5 Aug 2025
Pum_barcode13_1_combined_clean.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenamePum_barcode13_1_combined_clean.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167246
Total Bases128.8 Mbp
Sequences flagged as poor quality0
Sequence length500-26739
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)