Bismark report for: /home/shared/16TB_HDD_01/sr320/github/project-mytilus-methylation/output/25.1-93M-cov-recovery/reads/93M_R1.fastp-trim.fq.gz and /home/shared/16TB_HDD_01/sr320/github/project-mytilus-methylation/output/25.1-93M-cov-recovery/reads/93M_R2.fastp-trim.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-mytilus-methylation/output/22-genome-prep/ with the specified options: -q --score-min L,0,-0.6 -p 12 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 91219204 Number of paired-end alignments with a unique best hit: 46820590 Mapping efficiency: 51.3% Sequence pairs with no alignments under any condition: 29992887 Sequence pairs did not map uniquely: 14405727 Sequence pairs which were discarded because genomic sequence could not be extracted: 42 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 23427348 ((converted) top strand) GA/CT/CT: 9212 (complementary to (converted) top strand) GA/CT/GA: 8779 (complementary to (converted) bottom strand) CT/GA/GA: 23375209 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1894232195 Total methylated C's in CpG context: 25469289 Total methylated C's in CHG context: 2701527 Total methylated C's in CHH context: 13793830 Total methylated C's in Unknown context: 249763 Total unmethylated C's in CpG context: 196109623 Total unmethylated C's in CHG context: 303121205 Total unmethylated C's in CHH context: 1353036721 Total unmethylated C's in Unknown context: 5704557 C methylated in CpG context: 11.5% C methylated in CHG context: 0.9% C methylated in CHH context: 1.0% C methylated in unknown context (CN or CHN): 4.2% Bismark completed in 0d 23h 13m 16s