A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
/home/shared/16TB_HDD_01/sr320/github/project-lake-trout/analyses/08-nanoplot
General Statistics
| Sample Name | Median length | Mean length | Read N50 | Median Qual | Mean Qual | # Reads (K) | Total Bases (Mb) |
|---|---|---|---|---|---|---|---|
| bc2068 | 3778bp | 4224bp | 4541bp | 39.8 | 30.2 | 3287.6K | 13887.0Mb |
NanoStat
Reports various statistics for long read dataset in FASTQ, BAM, or albacore sequencing summary format (supports NanoPack; NanoPlot, NanoComp).https://github.com/wdecoster/nanostat; https://github.com/wdecoster/nanoplotDOI: 10.1093/bioinformatics/bty149
Programs are part of the NanoPack family for summarising results of sequencing on Oxford Nanopore methods (MinION, PromethION etc.)Summary Statistics (FASTQ)
| Sample Name | Median length | Mean length | Read N50 | Median Qual | Mean Qual | # Reads (K) | Total Bases (Mb) |
|---|---|---|---|---|---|---|---|
| bc2068 | 3778bp | 4224bp | 4541bp | 39.8 | 30.2 | 3287.6K | 13887.0Mb |
Reads by quality
Read counts categorised by read quality (Phred score).
Sequencing machines assign each generated read a quality score using the Phred scale. The phred score represents the liklelyhood that a given read contains errors. High quality reads have a high score.