A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
/home/shared/16TB_HDD_01/sr320/github/project-lake-trout/analyses/08-nanoplot
General Statistics
| Sample Name | Median length | Mean length | Read N50 | Median Qual | Mean Qual | # Reads (K) | Total Bases (Mb) |
|---|---|---|---|---|---|---|---|
| bc2041 | 6516bp | 7634bp | 8296bp | 38.8 | 29.0 | 3259.0K | 24879.4Mb |
| bc2068 | 3778bp | 4224bp | 4541bp | 39.8 | 30.2 | 3287.6K | 13887.0Mb |
| bc2069 | 6046bp | 6836bp | 7356bp | 38.9 | 29.0 | 1348.6K | 9219.5Mb |
| bc2070 | 5499bp | 6181bp | 6549bp | 39.2 | 29.4 | 3019.6K | 18663.5Mb |
| bc2071 | 5349bp | 6036bp | 6439bp | 39.3 | 29.4 | 3406.0K | 20559.9Mb |
| bc2072 | 5599bp | 6333bp | 6759bp | 39.2 | 29.3 | 2312.3K | 14643.5Mb |
| bc2073 | 5693bp | 6488bp | 6960bp | 39.1 | 29.2 | 3641.3K | 23624.5Mb |
| bc2096 | 5216bp | 6336bp | 7191bp | 38.8 | 29.1 | 5899.5K | 37377.0Mb |
NanoStat
Reports various statistics for long read dataset in FASTQ, BAM, or albacore sequencing summary format (supports NanoPack; NanoPlot, NanoComp).https://github.com/wdecoster/nanostat; https://github.com/wdecoster/nanoplotDOI: 10.1093/bioinformatics/bty149
Programs are part of the NanoPack family for summarising results of sequencing on Oxford Nanopore methods (MinION, PromethION etc.)Summary Statistics (FASTQ)
| Sample Name | Median length | Mean length | Read N50 | Median Qual | Mean Qual | # Reads (K) | Total Bases (Mb) |
|---|---|---|---|---|---|---|---|
| bc2041 | 6516bp | 7634bp | 8296bp | 38.8 | 29.0 | 3259.0K | 24879.4Mb |
| bc2068 | 3778bp | 4224bp | 4541bp | 39.8 | 30.2 | 3287.6K | 13887.0Mb |
| bc2069 | 6046bp | 6836bp | 7356bp | 38.9 | 29.0 | 1348.6K | 9219.5Mb |
| bc2070 | 5499bp | 6181bp | 6549bp | 39.2 | 29.4 | 3019.6K | 18663.5Mb |
| bc2071 | 5349bp | 6036bp | 6439bp | 39.3 | 29.4 | 3406.0K | 20559.9Mb |
| bc2072 | 5599bp | 6333bp | 6759bp | 39.2 | 29.3 | 2312.3K | 14643.5Mb |
| bc2073 | 5693bp | 6488bp | 6960bp | 39.1 | 29.2 | 3641.3K | 23624.5Mb |
| bc2096 | 5216bp | 6336bp | 7191bp | 38.8 | 29.1 | 5899.5K | 37377.0Mb |
Reads by quality
Read counts categorised by read quality (Phred score).
Sequencing machines assign each generated read a quality score using the Phred scale. The phred score represents the liklelyhood that a given read contains errors. High quality reads have a high score.