Bismark report for: ../data/trimmed-reads/SRR6995998_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 20934759 Number of alignments with a unique best hit from the different alignments: 8130788 Mapping efficiency: 38.8% Sequences with no alignments under any condition: 8520150 Sequences did not map uniquely: 4283821 Sequences which were discarded because genomic sequence could not be extracted: 1 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 212804 ((converted) top strand) CT/GA: 212676 ((converted) bottom strand) GA/CT: 3842291 (complementary to (converted) top strand) GA/GA: 3863016 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 152751530 Total methylated C's in CpG context: 13859673 Total methylated C's in CHG context: 5213123 Total methylated C's in CHH context: 18651831 Total methylated C's in Unknown context: 193345 Total unmethylated C's in CpG context: 15818700 Total unmethylated C's in CHG context: 31421214 Total unmethylated C's in CHH context: 67786989 Total unmethylated C's in Unknown context: 649735 C methylated in CpG context: 46.7% C methylated in CHG context: 14.2% C methylated in CHH context: 21.6% C methylated in Unknown context (CN or CHN): 22.9% Bismark completed in 0d 0h 26m 7s