Bismark report for: ../data/trimmed-reads/SRR6995996_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 28523919 Number of alignments with a unique best hit from the different alignments: 13703425 Mapping efficiency: 48.0% Sequences with no alignments under any condition: 8692432 Sequences did not map uniquely: 6128062 Sequences which were discarded because genomic sequence could not be extracted: 4 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 930172 ((converted) top strand) CT/GA: 925369 ((converted) bottom strand) GA/CT: 5910313 (complementary to (converted) top strand) GA/GA: 5937567 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 268009855 Total methylated C's in CpG context: 32939575 Total methylated C's in CHG context: 16120685 Total methylated C's in CHH context: 56291457 Total methylated C's in Unknown context: 387476 Total unmethylated C's in CpG context: 14054918 Total unmethylated C's in CHG context: 47114163 Total unmethylated C's in CHH context: 101489057 Total unmethylated C's in Unknown context: 843657 C methylated in CpG context: 70.1% C methylated in CHG context: 25.5% C methylated in CHH context: 35.7% C methylated in Unknown context (CN or CHN): 31.5% Bismark completed in 0d 0h 30m 36s