Bismark report for: ../data/trimmed-reads/SRR6995995_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 31440951 Number of alignments with a unique best hit from the different alignments: 16326352 Mapping efficiency: 51.9% Sequences with no alignments under any condition: 7192374 Sequences did not map uniquely: 7922225 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 681200 ((converted) top strand) CT/GA: 679514 ((converted) bottom strand) GA/CT: 7435535 (complementary to (converted) top strand) GA/GA: 7530103 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 323868126 Total methylated C's in CpG context: 48475583 Total methylated C's in CHG context: 17408351 Total methylated C's in CHH context: 57645394 Total methylated C's in Unknown context: 390474 Total unmethylated C's in CpG context: 19594014 Total unmethylated C's in CHG context: 64580248 Total unmethylated C's in CHH context: 116164536 Total unmethylated C's in Unknown context: 905448 C methylated in CpG context: 71.2% C methylated in CHG context: 21.2% C methylated in CHH context: 33.2% C methylated in Unknown context (CN or CHN): 30.1% Bismark completed in 0d 0h 31m 56s