Bismark report for: ../data/trimmed-reads/SRR6995994_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 2835345 Number of alignments with a unique best hit from the different alignments: 1191953 Mapping efficiency: 42.0% Sequences with no alignments under any condition: 908438 Sequences did not map uniquely: 734954 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 119895 ((converted) top strand) CT/GA: 134912 ((converted) bottom strand) GA/CT: 442850 (complementary to (converted) top strand) GA/GA: 494296 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 23862948 Total methylated C's in CpG context: 5226320 Total methylated C's in CHG context: 3007685 Total methylated C's in CHH context: 8531556 Total methylated C's in Unknown context: 36442 Total unmethylated C's in CpG context: 778082 Total unmethylated C's in CHG context: 2880476 Total unmethylated C's in CHH context: 3438829 Total unmethylated C's in Unknown context: 28704 C methylated in CpG context: 87.0% C methylated in CHG context: 51.1% C methylated in CHH context: 71.3% C methylated in Unknown context (CN or CHN): 55.9% Bismark completed in 0d 0h 2m 35s