Bismark report for: ../data/trimmed-reads/SRR6995993_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 21578440 Number of alignments with a unique best hit from the different alignments: 11084149 Mapping efficiency: 51.4% Sequences with no alignments under any condition: 5301433 Sequences did not map uniquely: 5192858 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 470774 ((converted) top strand) CT/GA: 478307 ((converted) bottom strand) GA/CT: 5024925 (complementary to (converted) top strand) GA/GA: 5110143 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 218245240 Total methylated C's in CpG context: 32500442 Total methylated C's in CHG context: 8246352 Total methylated C's in CHH context: 26330315 Total methylated C's in Unknown context: 221200 Total unmethylated C's in CpG context: 14466369 Total unmethylated C's in CHG context: 47850314 Total unmethylated C's in CHH context: 88851448 Total unmethylated C's in Unknown context: 636641 C methylated in CpG context: 69.2% C methylated in CHG context: 14.7% C methylated in CHH context: 22.9% C methylated in Unknown context (CN or CHN): 25.8% Bismark completed in 0d 0h 22m 15s