Bismark report for: ../data/trimmed-reads/SRR6995996_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.2 -p 8 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 159658 Mapping efficiency: 31.9% Sequences with no alignments under any condition: 259915 Sequences did not map uniquely: 80427 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 8588 ((converted) top strand) CT/GA: 8817 ((converted) bottom strand) GA/CT: 70811 (complementary to (converted) top strand) GA/GA: 71442 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3222221 Total methylated C's in CpG context: 423326 Total methylated C's in CHG context: 202109 Total methylated C's in CHH context: 684618 Total methylated C's in Unknown context: 1080 Total unmethylated C's in CpG context: 147725 Total unmethylated C's in CHG context: 569371 Total unmethylated C's in CHH context: 1195072 Total unmethylated C's in Unknown context: 1833 C methylated in CpG context: 74.1% C methylated in CHG context: 26.2% C methylated in CHH context: 36.4% C methylated in Unknown context (CN or CHN): 37.1% Bismark completed in 0d 0h 0m 49s