Bismark report for: ../data/trimmed-reads/SRR6995994_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.2 -p 8 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 110073 Mapping efficiency: 22.0% Sequences with no alignments under any condition: 315092 Sequences did not map uniquely: 74835 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 9976 ((converted) top strand) CT/GA: 10722 ((converted) bottom strand) GA/CT: 43116 (complementary to (converted) top strand) GA/GA: 46259 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2280418 Total methylated C's in CpG context: 506028 Total methylated C's in CHG context: 310939 Total methylated C's in CHH context: 834687 Total methylated C's in Unknown context: 810 Total unmethylated C's in CpG context: 58854 Total unmethylated C's in CHG context: 257388 Total unmethylated C's in CHH context: 312522 Total unmethylated C's in Unknown context: 394 C methylated in CpG context: 89.6% C methylated in CHG context: 54.7% C methylated in CHH context: 72.8% C methylated in Unknown context (CN or CHN): 67.3% Bismark completed in 0d 0h 0m 47s