Bismark report for: ../data/trimmed-reads/SRR6995993_trimmed.fq.gz (version: v0.25.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-gulf/data/genome/bismark/ with the specified options: -q --score-min L,0,-0.2 -p 8 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 500000 Number of alignments with a unique best hit from the different alignments: 153889 Mapping efficiency: 30.8% Sequences with no alignments under any condition: 266468 Sequences did not map uniquely: 79643 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 6066 ((converted) top strand) CT/GA: 6258 ((converted) bottom strand) GA/CT: 70268 (complementary to (converted) top strand) GA/GA: 71297 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3144550 Total methylated C's in CpG context: 504868 Total methylated C's in CHG context: 124661 Total methylated C's in CHH context: 385976 Total methylated C's in Unknown context: 742 Total unmethylated C's in CpG context: 172461 Total unmethylated C's in CHG context: 682064 Total unmethylated C's in CHH context: 1274520 Total unmethylated C's in Unknown context: 1531 C methylated in CpG context: 74.5% C methylated in CHG context: 15.5% C methylated in CHH context: 23.2% C methylated in Unknown context (CN or CHN): 32.6% Bismark completed in 0d 0h 0m 59s