SUMMARISING RUN PARAMETERS ========================= Input filename: SRR6995993.fastq.gz Trimming mode: single-end Trim Galore version: 2.3.0 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (user-specified or auto-detected) Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length single-end: 20 bp Poly-G trimming enabled: removing poly-G tails from 3' end of both R1 and R2 (2-colour sequencer artifact) Output file will be GZIP compressed Trim Galore 2.3.0 — adapter trimming built in This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR6995993.fastq.gz Processing reads on 1 core in single-end mode ... === Summary === Total reads processed: 25,752,634 Reads with adapters: 15,393,563 (59.8%) Reads written (passing filters): 25,752,634 (100.0%) Total basepairs processed: 2,601,016,034 bp Quality-trimmed: 168,252,428 bp (6.5%) Total written (filtered): 2,138,797,692 bp (82.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 15393563 times. No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Overview of removed sequences length count expect max.err error counts 1 7645997 6438158.5 0 7645997 2 534343 1609539.6 0 534343 3 291109 402384.9 0 291109 4 245902 100596.2 0 245902 5 222809 25149.1 0 222809 6 221201 6287.3 0 221201 7 227983 1571.8 0 227983 8 176189 393.0 0 176189 9 191705 98.2 0 191705 10 196403 24.6 1 196403 11 135410 6.1 1 135410 12 172032 1.5 1 172032 13 142443 0.4 1 142443 14 139453 0.1 1 139453 15 140261 0.0 1 140261 16 127613 0.0 1 127613 17 141045 0.0 1 141045 18 145508 0.0 1 145508 19 61757 0.0 1 61757 20 97023 0.0 2 97023 21 90106 0.0 2 90106 22 85550 0.0 2 85550 23 72642 0.0 2 72642 24 72920 0.0 2 72920 25 65492 0.0 2 65492 26 57563 0.0 2 57563 27 54733 0.0 2 54733 28 59928 0.0 2 59928 29 41393 0.0 2 41393 30 49108 0.0 3 49108 31 23838 0.0 3 23838 32 37226 0.0 3 37226 33 26212 0.0 3 26212 34 29751 0.0 3 29751 35 22545 0.0 3 22545 36 23175 0.0 3 23175 37 18672 0.0 3 18672 38 16053 0.0 3 16053 39 13969 0.0 3 13969 40 11682 0.0 4 11682 41 10476 0.0 4 10476 42 10739 0.0 4 10739 43 8515 0.0 4 8515 44 7287 0.0 4 7287 45 6867 0.0 4 6867 46 6170 0.0 4 6170 47 4626 0.0 4 4626 48 4038 0.0 4 4038 49 4377 0.0 4 4377 50 4703 0.0 5 4703 51 5286 0.0 5 5286 52 5055 0.0 5 5055 53 3891 0.0 5 3891 54 3728 0.0 5 3728 55 3839 0.0 5 3839 56 4944 0.0 5 4944 57 7121 0.0 5 7121 58 7219 0.0 5 7219 59 5116 0.0 5 5116 60 7817 0.0 6 7817 61 10532 0.0 6 10532 62 26665 0.0 6 26665 63 53960 0.0 6 53960 64 17759 0.0 6 17759 65 22284 0.0 6 22284 66 49172 0.0 6 49172 67 173170 0.0 6 173170 68 380144 0.0 6 380144 69 1270585 0.0 6 1270585 70 552214 0.0 7 552214 71 201199 0.0 7 201199 72 72347 0.0 7 72347 73 21280 0.0 7 21280 74 11117 0.0 7 11117 75 6492 0.0 7 6492 76 5119 0.0 7 5119 77 6705 0.0 7 6705 78 6642 0.0 7 6642 79 6064 0.0 7 6064 80 5659 0.0 8 5659 81 5193 0.0 8 5193 82 4999 0.0 8 4999 83 4639 0.0 8 4639 84 4373 0.0 8 4373 85 4621 0.0 8 4621 86 4757 0.0 8 4757 87 5135 0.0 8 5135 88 4925 0.0 8 4925 89 4758 0.0 8 4758 90 5314 0.0 9 5314 91 5626 0.0 9 5626 92 5942 0.0 9 5942 93 6196 0.0 9 6196 94 6672 0.0 9 6672 95 7356 0.0 9 7356 96 8416 0.0 9 8416 97 9216 0.0 9 9216 98 10374 0.0 9 10374 99 11835 0.0 9 11835 100 23941 0.0 10 23941 101 99608 0.0 10 99608 RUN STATISTICS FOR INPUT FILE: SRR6995993.fastq.gz ============================================= 25752634 sequences processed in total Sequences removed because they became shorter than the length cutoff of 20 bp: 3241121 (12.6%) Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) Sequences removed because of too many N bases: 0 (0.0%) Reads with poly-G/C trimmed: 287752 (1.1%); 1,190,135 bp removed