The exit status of the task that caused the workflow execution to fail was: 255.
The full error message was:
Error executing process > 'NFCORE_METHYLSEQ:FASTA_INDEX_BISMARK_BWAMETH:BISMARK_GENOMEPREPARATION (BismarkIndex/GCF_031168955.1_ASM3116895v1_genomic.fna)'
Caused by:
Process `NFCORE_METHYLSEQ:FASTA_INDEX_BISMARK_BWAMETH:BISMARK_GENOMEPREPARATION (BismarkIndex/GCF_031168955.1_ASM3116895v1_genomic.fna)` terminated with an error exit status (255)
Command executed:
bismark_genome_preparation \
--bowtie2 \
BismarkIndex
cat <<-END_VERSIONS > versions.yml
"NFCORE_METHYLSEQ:FASTA_INDEX_BISMARK_BWAMETH:BISMARK_GENOMEPREPARATION":
bismark: $(echo $(bismark -v 2>&1) | sed 's/^.*Bismark Version: v//; s/Copyright.*$//')
END_VERSIONS
Command exit status:
255
Command output:
(empty)
Command error:
INFO: Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred
INFO: Environment variable SINGULARITYENV_NXF_TASK_WORKDIR is set, but APPTAINERENV_NXF_TASK_WORKDIR is preferred
INFO: Environment variable SINGULARITYENV_NXF_DEBUG is set, but APPTAINERENV_NXF_DEBUG is preferred
Writing bisulfite genomes out into a single MFA (multi FastA) file
The specified genome folder /gscratch/scrubbed/srlab/nxf.C73Xfo0L1Y/BismarkIndex/ does not contain any sequence files in FastA format (with .fa, .fa.gz, .fasta or .fasta.gz file extensions)
Work dir:
/mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/output/17-nextflow/work/cb/b4af45e1e0dca774f79072d911f2f0
Container:
/gscratch/scrubbed/srlab/.apptainer/depot.galaxyproject.org-singularity-bismark-0.24.2--hdfd78af_0.img
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
nextflow run nf-core/methylseq -c /gscratch/scrubbed/sr320/github/project-cod-temperature/code/17.config --input /gscratch/scrubbed/sr320/github/project-cod-temperature/code/17.samplesheet.csv --outdir /gscratch/scrubbed/sr320/github/project-cod-temperature/output/17-nextflow --fasta /gscratch/scrubbed/sr320/github/project-cod-temperature/data/GCF_031168955.1_ASM3116895v1_genomic.fna -resume -with-trace -with-report nf_report.html -with-timeline nf_timeline.html --nomeseqf96ccc7395766f956718a67ea91ab7e7e1a19e15-8d87-44aa-89df-2179dcbf0c6bhttps://github.com/nf-core/methylseq, revision master (commit hash f3e4b1bf58f5da9a2788426bfb320d8ed81ead15)These plots give an overview of the distribution of resource usage for each process.
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