Nextflow workflow report

[intergalactic_meucci]

Workflow execution completed unsuccessfully!

The exit status of the task that caused the workflow execution to fail was: 255.

The full error message was:

Error executing process > 'NFCORE_METHYLSEQ:FASTA_INDEX_BISMARK_BWAMETH:BISMARK_GENOMEPREPARATION (BismarkIndex/GCF_031168955.1_ASM3116895v1_genomic.fna)'

Caused by:
  Process `NFCORE_METHYLSEQ:FASTA_INDEX_BISMARK_BWAMETH:BISMARK_GENOMEPREPARATION (BismarkIndex/GCF_031168955.1_ASM3116895v1_genomic.fna)` terminated with an error exit status (255)


Command executed:

  bismark_genome_preparation \
      --bowtie2 \
      BismarkIndex
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_METHYLSEQ:FASTA_INDEX_BISMARK_BWAMETH:BISMARK_GENOMEPREPARATION":
      bismark: $(echo $(bismark -v 2>&1) | sed 's/^.*Bismark Version: v//; s/Copyright.*$//')
  END_VERSIONS

Command exit status:
  255

Command output:
  (empty)

Command error:
  INFO:    Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred
  INFO:    Environment variable SINGULARITYENV_NXF_TASK_WORKDIR is set, but APPTAINERENV_NXF_TASK_WORKDIR is preferred
  INFO:    Environment variable SINGULARITYENV_NXF_DEBUG is set, but APPTAINERENV_NXF_DEBUG is preferred
  Writing bisulfite genomes out into a single MFA (multi FastA) file
  
  The specified genome folder /gscratch/scrubbed/srlab/nxf.C73Xfo0L1Y/BismarkIndex/ does not contain any sequence files in FastA format (with .fa, .fa.gz, .fasta or .fasta.gz file extensions)

Work dir:
  /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/output/17-nextflow/work/cb/b4af45e1e0dca774f79072d911f2f0

Container:
  /gscratch/scrubbed/srlab/.apptainer/depot.galaxyproject.org-singularity-bismark-0.24.2--hdfd78af_0.img

Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
Run times
14-Aug-2025 19:09:03 - 14-Aug-2025 19:11:32 (duration: 2m 29s)
  0 succeeded  
  0 cached  
  0 ignored  
  1 failed (2 retries)  
Nextflow command
nextflow run nf-core/methylseq -c /gscratch/scrubbed/sr320/github/project-cod-temperature/code/17.config --input /gscratch/scrubbed/sr320/github/project-cod-temperature/code/17.samplesheet.csv --outdir /gscratch/scrubbed/sr320/github/project-cod-temperature/output/17-nextflow --fasta /gscratch/scrubbed/sr320/github/project-cod-temperature/data/GCF_031168955.1_ASM3116895v1_genomic.fna -resume -with-trace -with-report nf_report.html -with-timeline nf_timeline.html --nomeseq
CPU-Hours
0.2 (100% failed)
Launch directory
/mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/output/17-nextflow
Work directory
/mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/output/17-nextflow/work
Project directory
/mmfs1/home/sr320/.nextflow/assets/nf-core/methylseq
Script name
main.nf
Script ID
f96ccc7395766f956718a67ea91ab7e7
Workflow session
e1a19e15-8d87-44aa-89df-2179dcbf0c6b
Workflow repository
https://github.com/nf-core/methylseq, revision master (commit hash f3e4b1bf58f5da9a2788426bfb320d8ed81ead15)
Workflow profile
standard
Nextflow version
version 25.04.6, build 5954 (01-07-2025 11:27 UTC)

Resource Usage

These plots give an overview of the distribution of resource usage for each process.

CPU

Memory

Job Duration

I/O

Tasks

This table shows information about each task in the workflow. Use the search box on the right to filter rows for specific values. Clicking headers will sort the table by that value and scrolling side to side will reveal more columns.

(tasks table omitted because the dataset is too big)