Bowtie 2 seems to be working fine (tested command 'bowtie2 --version' [2.5.4])
Output format is BAM (default)
Alignments will be written out in BAM format. Samtools found here: '/srlab/programs/samtools-1.20/samtools'
Reference genome folder provided is /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/data/genome/	(absolute path is '/mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/data/genome/)'
FastQ format assumed (by default)
Attention: early reports suggested that high values of -p  to have diminishing returns. Please test different values using a small subset of data for your hardware setting.
Each Bowtie 2 instance is going to be run with 8 threads. Please monitor performance closely and tune down if necessary!

Input files to be analysed (in current folder '/mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/output/16-bismark'):
/mmfs1/gscratch/coenv/sr320/cod-bs/21B_1.fastq.gz
/mmfs1/gscratch/coenv/sr320/cod-bs/21B_2.fastq.gz
Library is assumed to be strand-specific (directional), alignments to strands complementary to the original top or bottom strands will be ignored (i.e. not performed!)
Output will be written into the directory: /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/output/16-bismark/
Setting parallelization to single-threaded (default)

Summary of all aligner options:	-q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500
Current working directory is: /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/output/16-bismark

Now reading in and storing sequence information of the genome specified in: /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/data/genome/

Single-core mode: setting pid to 1

Paired-end alignments will be performed
=======================================

The provided filenames for paired-end alignments are /mmfs1/gscratch/coenv/sr320/cod-bs/21B_1.fastq.gz and /mmfs1/gscratch/coenv/sr320/cod-bs/21B_2.fastq.gz
Input files are in FastQ format
Writing a C -> T converted version of the input file 21B_1.fastq.gz to 21B_1.fastq.gz_C_to_T.fastq

Created C -> T converted version of the FastQ file 21B_1.fastq.gz (62652687 sequences in total)

Writing a G -> A converted version of the input file 21B_2.fastq.gz to 21B_2.fastq.gz_G_to_A.fastq

Created G -> A converted version of the FastQ file 21B_2.fastq.gz (62652687 sequences in total)

Input files are 21B_1.fastq.gz_C_to_T.fastq and 21B_2.fastq.gz_G_to_A.fastq (FastQ)
Now running 2 instances of Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/data/genome/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500

Now starting a Bowtie 2 paired-end alignment for CTread1GAread2CTgenome (reading in sequences from 21B_1.fastq.gz_C_to_T.fastq and 21B_2.fastq.gz_G_to_A.fastq, with the options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 --norc))
Found first alignment:
LH00160:538:22WG5VLT4:6:1101:23553:1042_1:N:0:CGGCTAAT+CTNGTTCT/1	77	*	0	0	*	*	0	0	GNGTAGTATTTTTTGGATAGGTAGGAATTTTTTTTTGAGGGTTATTATTGGATTGTGTTGGATTAATTGTATTTAAGTTATTTTAAGTGTTTTGTTTGTTGTTGTTGGTTTATTTTAGTTGTTATTGTAATGATTGTTTTTTGTTGATGTG	I#9IIIIIIIIIIIII9II9-IIIIIIIIIIIIIIII-IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII-IIIIIIIIIIIIIIIIIIII9IIIIIIIIIIII9IIIIII9IIIIIIIIIIIIIIII9IIIIIIIIIIIIII-IIII	YT:Z:UP
LH00160:538:22WG5VLT4:6:1101:23553:1042_2:N:0:CGGCTAAT+CTNGTTCT/2	141	*	0	0	*	*	0	0	AAAAAAAAAAAAATAAACACATCAACAAAAAACAATCATTACAATAACAACTAAAATAAACCAACAACAACAAACAAAACACTTAAAATAACTTTAATACAATTAATCCAACACAATCCAATAATAAACCTCAAAAAAAAATTCCTACCTA	IIIIIIIIIIIIIIII-II9IIIIIII9IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII-IIIIIIII-IIIIIIIII-III-IIIIIII9IIIIIIIIIIIIIIIIIIIIIIIIIIIIIII-IIIIIII-IIIIIIIII9IIII9	YT:Z:UP
Now starting a Bowtie 2 paired-end alignment for CTread1GAread2GAgenome (reading in sequences from 21B_1.fastq.gz_C_to_T.fastq and 21B_2.fastq.gz_G_to_A.fastq, with the options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 --nofw))
Found first alignment:
LH00160:538:22WG5VLT4:6:1101:23553:1042_1:N:0:CGGCTAAT+CTNGTTCT/1	83	NC_082382.1_GA_converted	6659783	40	151M	=	6659763	-171	CACATCAACAAAAAACAATCATTACAATAACAACTAAAATAAACCAACAACAACAAACAAAACACTTAAAATAACTTAAATACAATTAATCCAACACAATCCAATAATAACCCTCAAAAAAAAATTCCTACCTATCCAAAAAATACTACNC	IIII-IIIIIIIIIIIIII9IIIIIIIIIIIIIIII9IIIIII9IIIIIIIIIIII9IIIIIIIIIIIIIIIIIIII-IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII-IIIIIIIIIIIIIIII-9II9IIIIIIIIIIIII9#I	AS:i:-7	XN:i:0	XM:i:2	XO:i:0	XG:i:0	NM:i:2	MD:Z:77T71A1	YS:i:-44	YT:Z:CP
LH00160:538:22WG5VLT4:6:1101:23553:1042_2:N:0:CGGCTAAT+CTNGTTCT/2	163	NC_082382.1_GA_converted	6659763	40	6M3D145M	=	6659783	171	AAAAAAAAAAAAATAAACACATCAACAAAAAACAATCATTACAATAACAACTAAAATAAACCAACAACAACAAACAAAACACTTAAAATAACTTTAATACAATTAATCCAACACAATCCAATAATAAACCTCAAAAAAAAATTCCTACCTA	IIIIIIIIIIIIIIII-II9IIIIIII9IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII-IIIIIIII-IIIIIIIII-III-IIIIIII9IIIIIIIIIIIIIIIIIIIIIIIIIIIIIII-IIIIIII-IIIIIIIII9IIII9	AS:i:-44	XN:i:0	XM:i:5	XO:i:1	XG:i:3	NM:i:8	MD:Z:4C1^CTT0T1T3T114C23	YS:i:-7	YT:Z:CP

>>> Writing bisulfite mapping results to 21B_pe.bam <<<


Reading in the sequence files /mmfs1/gscratch/coenv/sr320/cod-bs/21B_1.fastq.gz and /mmfs1/gscratch/coenv/sr320/cod-bs/21B_2.fastq.gz
Processed 1000000 sequence pairs so far