Bismark report for: /mmfs1/gscratch/coenv/sr320/cod-bs/06B_1.fastq.gz and /mmfs1/gscratch/coenv/sr320/cod-bs/06B_2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/data/genome/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 51326454 Number of paired-end alignments with a unique best hit: 35981384 Mapping efficiency: 70.1% Sequence pairs with no alignments under any condition: 10255565 Sequence pairs did not map uniquely: 5089505 Sequence pairs which were discarded because genomic sequence could not be extracted: 50 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 17966826 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 18014508 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2278727614 Total methylated C's in CpG context: 242827929 Total methylated C's in CHG context: 15034207 Total methylated C's in CHH context: 52630375 Total methylated C's in Unknown context: 4809378 Total unmethylated C's in CpG context: 81108635 Total unmethylated C's in CHG context: 452073792 Total unmethylated C's in CHH context: 1435052676 Total unmethylated C's in Unknown context: 7627131 C methylated in CpG context: 75.0% C methylated in CHG context: 3.2% C methylated in CHH context: 3.5% C methylated in Unknown context (CN or CHN): 38.7% Bismark completed in 0d 4h 8m 9s