Bismark report for: /mmfs1/gscratch/coenv/sr320/cod-bs/03B_1.fastq.gz and /mmfs1/gscratch/coenv/sr320/cod-bs/03B_2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-cod-temperature/data/genome/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 52740330 Number of paired-end alignments with a unique best hit: 37055762 Mapping efficiency: 70.3% Sequence pairs with no alignments under any condition: 10545459 Sequence pairs did not map uniquely: 5139109 Sequence pairs which were discarded because genomic sequence could not be extracted: 62 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 18497701 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 18557999 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2348843863 Total methylated C's in CpG context: 244927516 Total methylated C's in CHG context: 15312678 Total methylated C's in CHH context: 53564039 Total methylated C's in Unknown context: 4756331 Total unmethylated C's in CpG context: 87259909 Total unmethylated C's in CHG context: 466272303 Total unmethylated C's in CHH context: 1481507418 Total unmethylated C's in Unknown context: 7775025 C methylated in CpG context: 73.7% C methylated in CHG context: 3.2% C methylated in CHH context: 3.5% C methylated in Unknown context (CN or CHN): 38.0% Bismark completed in 0d 3h 53m 34s