Bismark report for: /mmfs1/gscratch/scrubbed/strigg/analyses/20250731_methylseq/raw-reads/LCo_BSr2_R1.fastq.gz and /mmfs1/gscratch/scrubbed/strigg/analyses/20250731_methylseq/raw-reads/LCo_BSr2_R2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-chilean-mussel/data/Mchi/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 100000000 Number of paired-end alignments with a unique best hit: 60875995 Mapping efficiency: 60.9% Sequence pairs with no alignments under any condition: 24043733 Sequence pairs did not map uniquely: 15080272 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 30497743 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 30378245 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2661442491 Total methylated C's in CpG context: 50458999 Total methylated C's in CHG context: 9841039 Total methylated C's in CHH context: 48270635 Total methylated C's in Unknown context: 1856194 Total unmethylated C's in CpG context: 248851060 Total unmethylated C's in CHG context: 402932871 Total unmethylated C's in CHH context: 1901087887 Total unmethylated C's in Unknown context: 9879125 C methylated in CpG context: 16.9% C methylated in CHG context: 2.4% C methylated in CHH context: 2.5% C methylated in Unknown context (CN or CHN): 15.8% Bismark completed in 0d 4h 47m 58s