Bowtie 2 seems to be working fine (tested command 'bowtie2 --version' [2.5.4]) Output format is BAM (default) Alignments will be written out in BAM format. Samtools found here: '/srlab/programs/samtools-1.20/samtools' Failed to move to ../../data/Mchi/: No such file or directory USAGE: bismark [options] {-1 -2 | } [] (--help for more details)