Bismark report for: /mmfs1/gscratch/scrubbed/strigg/analyses/20250731_methylseq/raw-reads/LYa_BSr1_R1.fastq and /mmfs1/gscratch/scrubbed/strigg/analyses/20250731_methylseq/raw-reads/LYa_BSr1_R2.fastq (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-chilean-mussel/data/Mchi/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000000 Number of paired-end alignments with a unique best hit: 60097814 Mapping efficiency: 60.1% Sequence pairs with no alignments under any condition: 25659847 Sequence pairs did not map uniquely: 14242339 Sequence pairs which were discarded because genomic sequence could not be extracted: 6 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 30093814 ((converted) top strand) GA/CT/CT: 11012 (complementary to (converted) top strand) GA/CT/GA: 9179 (complementary to (converted) bottom strand) CT/GA/GA: 29983803 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2630781380 Total methylated C's in CpG context: 49063397 Total methylated C's in CHG context: 9766412 Total methylated C's in CHH context: 49410400 Total methylated C's in Unknown context: 1844862 Total unmethylated C's in CpG context: 244161809 Total unmethylated C's in CHG context: 399774079 Total unmethylated C's in CHH context: 1878605283 Total unmethylated C's in Unknown context: 10211829 C methylated in CpG context: 16.7% C methylated in CHG context: 2.4% C methylated in CHH context: 2.6% C methylated in Unknown context (CN or CHN): 15.3% Bismark completed in 0d 5h 3m 41s