Bismark report for: /mmfs1/gscratch/scrubbed/strigg/analyses/20250731_methylseq/raw-reads/LCo_BSr2_R1.fastq and /mmfs1/gscratch/scrubbed/strigg/analyses/20250731_methylseq/raw-reads/LCo_BSr2_R2.fastq (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-chilean-mussel/data/Mchi/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000000 Number of paired-end alignments with a unique best hit: 60878996 Mapping efficiency: 60.9% Sequence pairs with no alignments under any condition: 24039187 Sequence pairs did not map uniquely: 15081817 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 30491328 ((converted) top strand) GA/CT/CT: 8645 (complementary to (converted) top strand) GA/CT/GA: 8373 (complementary to (converted) bottom strand) CT/GA/GA: 30370643 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2662989642 Total methylated C's in CpG context: 50467104 Total methylated C's in CHG context: 9852043 Total methylated C's in CHH context: 49590559 Total methylated C's in Unknown context: 1862578 Total unmethylated C's in CpG context: 248835264 Total unmethylated C's in CHG context: 402913657 Total unmethylated C's in CHH context: 1901331015 Total unmethylated C's in Unknown context: 9882325 C methylated in CpG context: 16.9% C methylated in CHG context: 2.4% C methylated in CHH context: 2.5% C methylated in Unknown context (CN or CHN): 15.9% Bismark completed in 0d 5h 10m 30s