FastQCFastQC Report
Sat 30 Aug 2025
Caligus_Female_22_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCaligus_Female_22_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55000000
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC16

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATGGTATCTAGTA2895710.5264927272727273TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATGGTATCTAGTG840030.15273272727272727TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)