/opt/ohpc/admin/lmod/lmod/init/bash: line 93: LD_LIBRARY_PATH: unbound variable
start 2026-09-27T12:54:37Z host n3447 sample=HC18_Triton_Wild_16 location=HC18_Triton_Wild
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 1066668 sequences (160000200 bp)...
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 241385, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.92, 37.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 709.065 CPU sec, 48.102 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 241813, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (124.42, 38.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 302)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 284, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1920)
[M::mem_pestat] mean and std.dev: (312.36, 416.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2543)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 1066668 reads in 710.120 CPU sec, 46.261 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 241234, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (124.78, 37.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 302)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 910, 1892)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5220)
[M::mem_pestat] mean and std.dev: (1161.90, 1232.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6884)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 1066668 reads in 713.551 CPU sec, 47.232 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 241907, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.83, 37.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 722.633 CPU sec, 47.213 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 241615, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (125.06, 37.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 95, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256)
[M::mem_pestat] mean and std.dev: (143.70, 166.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1662)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 718.481 CPU sec, 46.638 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 241291, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (124.76, 37.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 302)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 327, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619)
[M::mem_pestat] mean and std.dev: (244.75, 195.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2142)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 722.599 CPU sec, 47.051 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 241323, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 121, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.53, 37.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 710.229 CPU sec, 46.420 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 241699, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.16, 37.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 708.702 CPU sec, 46.073 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 242332, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (124.49, 37.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 716.450 CPU sec, 46.635 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 241549, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (124.76, 37.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 302)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 381, 1409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4179)
[M::mem_pestat] mean and std.dev: (527.55, 578.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5564)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 734.300 CPU sec, 47.806 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 242013, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (125.08, 37.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1137, 2271, 6303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16635)
[M::mem_pestat] mean and std.dev: (3632.18, 3103.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21801)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 743.205 CPU sec, 48.357 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 240931, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.92, 37.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 743.723 CPU sec, 48.635 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 240219, 15, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (97, 121, 147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247)
[M::mem_pestat] mean and std.dev: (123.22, 37.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 297)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 208, 881)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2409)
[M::mem_pestat] mean and std.dev: (456.50, 528.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3173)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 729.665 CPU sec, 47.525 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 241692, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 121, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.70, 37.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (666, 1122, 2953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7527)
[M::mem_pestat] mean and std.dev: (1542.00, 1488.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9814)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 731.658 CPU sec, 47.975 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 241437, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.11, 37.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 731.485 CPU sec, 47.434 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 241799, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.73, 37.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 712.176 CPU sec, 46.113 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 241182, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.78, 37.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 107, 6123)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18319)
[M::mem_pestat] mean and std.dev: (2036.08, 3415.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 24417)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 734.519 CPU sec, 47.633 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 240714, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 121, 147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245)
[M::mem_pestat] mean and std.dev: (123.43, 37.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 294)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (492, 1903, 7347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21057)
[M::mem_pestat] mean and std.dev: (3600.27, 3510.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27912)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 726.626 CPU sec, 47.031 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 241132, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (97, 121, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250)
[M::mem_pestat] mean and std.dev: (123.57, 37.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 301)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (54, 5164, 5368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15996)
[M::mem_pestat] mean and std.dev: (3746.40, 3358.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21310)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 1066668 reads in 719.135 CPU sec, 47.845 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 241617, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.87, 37.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 717.394 CPU sec, 46.211 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 241812, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.29, 37.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 723.147 CPU sec, 46.931 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 241703, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.71, 37.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 722.862 CPU sec, 47.781 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 241292, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (124.76, 37.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 302)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 459, 1646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4750)
[M::mem_pestat] mean and std.dev: (718.78, 902.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6302)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 732.178 CPU sec, 48.093 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 240134, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.83, 37.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 727.178 CPU sec, 47.716 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 241028, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.26, 37.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 728.644 CPU sec, 47.528 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 241184, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.31, 37.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 169, 6391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19085)
[M::mem_pestat] mean and std.dev: (1954.73, 2856.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25432)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 726.194 CPU sec, 47.240 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 241653, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.94, 37.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 453, 2023)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5555)
[M::mem_pestat] mean and std.dev: (901.73, 1095.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7321)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 725.692 CPU sec, 47.232 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 240526, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (125.07, 37.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 735.356 CPU sec, 47.640 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 240461, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.95, 37.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 724.175 CPU sec, 47.076 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 239567, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.12, 37.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 723.524 CPU sec, 47.366 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 240884, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246)
[M::mem_pestat] mean and std.dev: (124.36, 37.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 721.839 CPU sec, 46.731 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 241294, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.36, 37.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 493, 2711)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8035)
[M::mem_pestat] mean and std.dev: (1409.92, 1744.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10697)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 725.240 CPU sec, 47.155 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 241224, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (125.10, 37.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 725.847 CPU sec, 47.224 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 239793, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.26, 37.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (269, 802, 2112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5798)
[M::mem_pestat] mean and std.dev: (944.33, 971.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 731.270 CPU sec, 47.374 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 240088, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.69, 37.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 721.564 CPU sec, 46.904 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 240778, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245)
[M::mem_pestat] mean and std.dev: (123.55, 37.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 742.864 CPU sec, 48.642 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 240830, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.09, 37.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 746.686 CPU sec, 47.936 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 241389, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.74, 37.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 153, 3535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10523)
[M::mem_pestat] mean and std.dev: (1448.46, 2665.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14017)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 749.167 CPU sec, 48.453 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 240010, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.68, 37.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 765.037 CPU sec, 49.587 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 240523, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 121, 147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245)
[M::mem_pestat] mean and std.dev: (123.41, 37.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 294)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (430, 710, 5820)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16600)
[M::mem_pestat] mean and std.dev: (2569.82, 3148.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21990)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 750.335 CPU sec, 48.833 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 240796, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (123.71, 37.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 743.484 CPU sec, 48.200 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 240998, 12, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (124.00, 37.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 220, 3973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11723)
[M::mem_pestat] mean and std.dev: (1510.50, 2338.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15598)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 746.549 CPU sec, 48.317 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 240860, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (124.99, 37.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 757.104 CPU sec, 49.182 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 240008, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249)
[M::mem_pestat] mean and std.dev: (125.01, 37.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 428, 1759)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5013)
[M::mem_pestat] mean and std.dev: (955.50, 1180.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6640)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 215328 sequences (32299200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 762.657 CPU sec, 49.409 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 48523, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 123, 150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252)
[M::mem_pestat] mean and std.dev: (125.46, 37.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 215328 reads in 155.689 CPU sec, 10.157 real sec
[main] Version: 0.7.19-r1273
[main] CMD: bwa mem -t 16 -R @RG\tID:HC18_Triton_Wild_16\tSM:HC18_Triton_Wild_16\tLB:HC18_Triton_Wild_16\tPL:ILLUMINA output/05_realign_xbOstLuri2/reference/GCA_061535525.1_xbOstLuri2_USDA-ARS_PSRF_primary_genomic.fna data/raw/HC18_Triton_Wild_16_S112_L004_R1_001.fastq.gz data/raw/HC18_Triton_Wild_16_S112_L004_R2_001.fastq.gz
[main] Real time: 2129.517 sec; CPU: 32276.450 sec
[bam_sort_core] merging from 2 files and 8 in-memory blocks...
end 2026-09-27T13:34:17Z sample=HC18_Triton_Wild_16