/opt/ohpc/admin/lmod/lmod/init/bash: line 93: LD_LIBRARY_PATH: unbound variable
start 2026-09-27T12:52:01Z host n3434 sample=HC18_Triton_Wild_10 location=HC18_Triton_Wild
[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13290, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.38, 29.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 286.325 CPU sec, 25.915 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13343, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.18, 29.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 287.209 CPU sec, 19.483 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13490, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (51.44, 28.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 286.880 CPU sec, 19.590 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13240, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.66, 29.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 284.439 CPU sec, 18.723 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13597, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.38, 29.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 288.887 CPU sec, 19.393 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13290, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.18, 29.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 290.267 CPU sec, 19.350 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13365, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.55, 29.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 269.271 CPU sec, 18.186 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13452, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.18, 29.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 264.859 CPU sec, 17.454 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13287, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.28, 29.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 263.666 CPU sec, 17.716 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13257, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.59, 29.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 263.291 CPU sec, 17.242 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13032, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.48, 29.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 281.997 CPU sec, 18.937 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13460, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (51.93, 29.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 288.537 CPU sec, 19.553 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13521, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.22, 29.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 283.925 CPU sec, 18.680 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 13273, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (51.58, 28.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 286.695 CPU sec, 19.020 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13601, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (51.46, 29.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 283.052 CPU sec, 18.726 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13505, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 72)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160)
[M::mem_pestat] mean and std.dev: (52.86, 30.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 288.048 CPU sec, 18.908 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13139, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.25, 29.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 291.975 CPU sec, 19.639 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13396, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.10, 29.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 289.715 CPU sec, 19.451 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 13358, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.31, 29.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 288.070 CPU sec, 19.209 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13416, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.25, 29.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 284.505 CPU sec, 18.600 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13325, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (51.68, 29.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 287.932 CPU sec, 19.442 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 13222, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.77, 30.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 290.493 CPU sec, 19.185 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13164, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.42, 29.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 293.640 CPU sec, 19.749 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13281, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.18, 29.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 291.724 CPU sec, 19.218 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 13327, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.40, 29.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 288.925 CPU sec, 19.467 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13048, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (51.81, 28.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 291.204 CPU sec, 19.266 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 12933, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 44, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163)
[M::mem_pestat] mean and std.dev: (53.72, 30.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 290.620 CPU sec, 19.572 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13204, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.12, 29.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 291.896 CPU sec, 19.295 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13355, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.40, 29.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 291.610 CPU sec, 19.621 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13271, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.56, 29.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 288.651 CPU sec, 18.949 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 13231, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (51.72, 29.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 290.621 CPU sec, 19.339 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13172, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (52.40, 29.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 295.196 CPU sec, 19.884 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13162, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (51.78, 29.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 294.788 CPU sec, 19.584 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13420, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (51.96, 29.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 293.385 CPU sec, 19.742 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13415, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (51.83, 29.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 290.839 CPU sec, 19.179 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13271, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.65, 29.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 287.515 CPU sec, 19.155 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13130, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.05, 29.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 291.838 CPU sec, 19.539 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 12874, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 44, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157)
[M::mem_pestat] mean and std.dev: (53.04, 30.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 294.059 CPU sec, 19.351 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13359, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (51.92, 29.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 290.475 CPU sec, 19.431 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13599, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (51.80, 29.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_process_seqs] Processed 1066668 reads in 291.126 CPU sec, 19.043 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 13103, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.13, 29.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 289.294 CPU sec, 19.477 real sec
[M::process] read 1066668 sequences (160000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 13557, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (52.36, 29.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1066668 reads in 291.280 CPU sec, 19.416 real sec
[M::process] read 989644 sequences (148446600 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 12028, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (51.55, 29.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 989644 reads in 273.299 CPU sec, 18.197 real sec
[main] Version: 0.7.19-r1273
[main] CMD: bwa mem -t 16 -R @RG\tID:HC18_Triton_Wild_10\tSM:HC18_Triton_Wild_10\tLB:HC18_Triton_Wild_10\tPL:ILLUMINA output/05_realign_xbOstLuri2/reference/GCA_061535525.1_xbOstLuri2_USDA-ARS_PSRF_primary_genomic.fna data/raw/HC18_Triton_Wild_10_S111_L004_R1_001.fastq.gz data/raw/HC18_Triton_Wild_10_S111_L004_R2_001.fastq.gz
[main] Real time: 879.030 sec; CPU: 12362.437 sec
[bam_sort_core] merging from 2 files and 8 in-memory blocks...
end 2026-09-27T13:09:56Z sample=HC18_Triton_Wild_10