Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-50_preproc.map (2812767 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 4560.38334968854 4839.82275036523 7928.8036747238 6578.19655350444 13039.1298794906 10679.3932033483 14809.305709199 25276.8665930712 31655.2718306949 734310.462157769 23033.0073633484 34954.6343633484 15090.5950535645 17574.5215268345 15088.1446619046 45923.603110722 12058.0568630709 20739.4252808058 7040.80701709245 13446.1781187858 10322.7140100292 8389.78156462149 9919.82797048369 3863.7996005403 3853.40892085007 18192.2605815657 1705.795529431 1470.46352818362 430.173389564515 0 66.8015389395802 29112 219438 2 4329.70210472758 4176.89575261608 8194.94861170494 6578.36469916525 11845.3249251738 13163.9453014451 13590.0089246724 22147.3382040898 32571.6497084206 677885.09134454 19960.3372268942 33452.8256731407 12232.4384367624 21846.7465581619 15121.3607165447 28404.0086071155 13795.4821575517 24985.4157530444 6434.69591902674 12101.0380809469 9280.23500487441 8671.07817701831 8028.61190595801 6491.54149891707 3989.75289589268 15991.3469928902 2342.3609716363 1357.90091263407 532.78305672743 0 74.8691392627583 28936 217365 3 4942.88063065965 4132.68535645644 8156.60449729725 6741.86475220255 12679.1078667316 11107.7259418269 15315.1264026189 20825.2282768393 36369.9420268605 713349.582367812 28864.0363251486 29318.7955682131 12931.7026439879 18475.6663379604 17721.0284475631 29161.3365438654 13250.4014964689 25786.7967921864 7357.31285568729 12940.4807165975 9958.55361487468 8204.99500696813 8374.73558607038 5716.85610150347 3093.29277364029 15880.2255188423 2008.16281399148 1242.38056186419 525.590625460029 0 75.9631518041426 29801 228737 4 4356.06699729191 4789.71776868979 7856.62053694697 6302.39336564385 11829.0080491659 15875.5065870477 14793.2173200592 27607.7231037789 33007.330192993 728016.101304724 20573.4513765794 36669.8921404045 13528.4606160771 23651.9361317683 12821.573999445 37901.4206352916 13638.3522565349 24186.3964773876 9569.10514651271 18243.4681743171 9508.02796383414 6826.5409334849 10112.5400032719 4878.26872923844 6232.38578242571 16581.478126387 1854.70508002532 1896.63399971954 401.223331234291 0 70.5086905010674 29062 222746 5 4925.77688541585 4732.29693101737 8333.7255458699 5545.19785674432 10680.6132090266 17660.7259253577 12240.9178812416 24143.7530788924 24787.598168247 805152.738532874 27903.9758557016 32629.4640890395 17092.5573654331 24131.6736147374 12956.4355953001 28785.6931340312 13774.5981371761 28085.4849585423 8569.5490507007 11480.9414108508 11133.2369316033 8172.27807725078 12335.5695398923 5865.63500401446 2763.43342180249 15490.6796445464 2273.93802144212 1534.63030677264 593.925222953137 0 89.3178510480203 28555 205277 6 4883.65665662015 5184.52686216838 7031.12848881488 4875.67005142135 10805.8670665236 13074.1524879457 13984.2737257831 24422.7327681335 23139.7939486546 706562.154103271 30102.2613486441 28252.6784484621 13785.0060086447 19333.1009064611 20679.6407164955 19455.3003953171 11545.2108611348 27406.5036329896 7314.46561536879 11023.0565232462 9526.90262678964 6928.96399117629 9654.30411056076 3955.57862923575 2715.06817722727 16176.6569971374 1721.48130658872 1158.00920326586 302.712013416071 0 85.1147788676934 29098 226484 7 5430.52649320744 4198.94258724215 9515.35256499632 6493.47228161485 12085.4223526683 11388.9195618017 14123.0590394363 20308.4037653843 24878.2204652133 853934.490209049 19993.3777930315 29493.6314220726 14648.8262631747 17231.7213084348 14902.2116823027 18447.5142602082 12305.4887254176 21856.7206479611 6863.31423497022 15017.4103523006 8873.30169282609 5935.74609760855 12269.7111629691 5149.68510642057 3001.42537704968 16626.2331606496 2441.5274395853 1324.89848240692 443.067657766509 0 127.019637573328 29176 225030 8 5371.3674730812 4972.64241301047 9345.3197405002 6303.75663266131 13250.5305723715 14513.505929836 12906.2019532257 20577.4219184024 26589.109446734 744027.215758626 37612.2791669662 29670.7001445169 12063.696508387 21075.8666069435 17319.9558478971 54933.02798104 12553.4493972952 25515.2631393934 7836.30781329304 11146.0491277589 8305.3403299367 6782.18831166236 10707.3794220282 5699.06141146862 2732.27131231461 16741.9845418581 2006.99353788492 1587.45171701625 211.422115857198 0 59.2905790636254 28814 213630 9 3898.37147823312 3855.18297564423 7382.71850347028 5203.08391683168 11500.9809969252 13226.5343414896 13381.3081472042 21575.756758334 33235.7669636588 853616.299051442 20323.8133192328 36468.9953885008 11183.0793154039 16945.4748128201 14669.6942640426 44777.7252958688 12205.2697823047 25053.9192137866 7793.81248485727 13364.5438196897 9726.12130778817 8616.96640906985 13579.878600047 3790.71890935501 3144.91567962594 13746.417267073 2178.46609208328 1128.3925218939 653.043788386167 0 76.0322585232695 29423 228148 10 5144.72205490211 5332.13422927378 7563.95878174783 5371.74651242063 11386.7549390736 11141.084833716 14530.0406429397 21744.3605017058 24470.5151273401 700802.460149748 21211.4728580765 35248.9539279462 12479.8075708362 19013.2930108798 16203.8422729216 9999.76986072646 12965.4874059067 23571.9701741576 7038.70259260839 17168.804807624 9956.32577849111 7758.23104370788 9801.08730143963 4514.3813030248 3209.91936531819 15341.7024330026 2145.00017172379 1226.22897640345 214.448015312709 0 88.3840978749866 29040 215323 11 3966.71425104751 4802.28782724905 7750.85293627038 5565.42610112543 13336.2879348984 13918.0748654435 13750.427139929 22730.6971583176 33476.5917588063 840978.082969746 28229.1775882564 36053.5465426005 13171.3097904388 25694.4078854114 16045.4341450336 9498.06539956811 13615.8752380718 24856.2752505822 8022.9678535091 11636.7588104632 10413.9632489741 8766.42540154203 13070.1508225804 4808.39454757818 2693.81751980424 14441.395712984 2612.34840015749 1345.87417631413 336.692708678214 0 87.8037795157571 28670 223493 12 4155.753723986 4286.50068639287 8738.66806379245 4411.35720694975 12962.96791212 14178.193688522 11493.2032599916 23583.5095428995 32951.3972169648 740654.96357311 31539.3282543966 32856.7128762559 9991.51064969079 18311.0006692221 14564.9984446717 36018.5996172895 14374.7746192339 21751.6258088735 7476.77921984313 10981.6636409088 9691.01614172825 6126.38568861572 9952.01128579237 4418.66577633731 3261.67058683643 14511.5533505318 1817.64001304436 1118.12846422114 978.893305529502 0 71.6647056535305 29077 233046 13 4620.13629226238 4705.97414684163 12353.0806530297 5975.99806769028 12957.5163761441 17130.3847140366 13779.7414040563 27239.3088869491 25326.1868399356 770104.752750989 32291.4087967726 35298.8916940915 14182.1064487747 19253.2651119904 16953.1096117875 27961.2094210472 11945.0634482244 22634.1323557921 7562.76099390097 17778.8736063755 8546.64201147602 9131.04512595371 10451.1063258984 4211.34826256013 2779.70466142718 17593.7153609956 1886.37776162851 1251.60454941172 327.605783022725 0 84.8682978036194 29656 223364 14 5025.63777742643 4665.38103152295 8295.69337321351 7998.44045787119 11253.346339461 14486.0223786124 14202.0355486427 23241.3112385124 24177.4832047832 838280.480895507 29207.4048256739 34080.3734631806 13308.8477435811 21056.6497955087 12902.8723411112 28448.0615405681 12217.5643807311 26850.215493561 7869.08884931219 12308.7342604779 9847.05964010779 7359.99317178602 13506.8410423925 5645.57891788415 3943.22015240172 13289.1362048574 2472.66477553126 1757.07835705123 536.705378930897 0 94.8333010899886 29107 226004 15 5379.20779693142 5172.07443188655 7743.21062116455 5355.71681173497 11984.90474616 15840.4855387057 13805.3788486057 23975.4954683786 23602.6618518882 821758.496907765 19747.8522373765 33865.5688105965 16159.9739022879 19402.1970904067 17050.585534607 9363.51234489272 11694.9590474221 20722.9483320874 7462.74920817485 11271.3812385315 7846.42643982703 8394.65689555203 11181.7629830183 6357.32518041847 5097.95185274163 13814.4843418224 1717.60495139082 1482.02049785659 285.444144606241 0 108.63381568963 29200 226854 16 5079.56029060712 4315.27408022325 10410.8727025002 5343.96977036358 12904.8977560126 13217.9021137702 14003.9922464442 24497.7940867527 26018.8121749703 824622.744770516 20163.6169662753 35606.8978030996 11732.3327782044 18117.2741945454 18202.7741747042 19250.4027929731 16232.2450988526 24679.3392448098 8618.53007702427 13479.780663265 11104.226907447 8999.73680933387 12973.0957048915 4521.19227108159 3185.542864583 15495.4653390044 2320.56580971128 1503.77457259563 310.064989930146 0 103.045161830401 28677 212485 17 4919.16058284894 4133.61066995391 7838.5197869119 4307.25032534899 10365.8178700581 13447.9335296349 14204.3311595455 27032.7999692447 42212.8813071729 790806.567758704 19317.1880718188 33806.1232701139 12747.6551382912 22885.2946365208 13325.1438776458 9603.07664083539 13228.4271715231 27592.8901208419 8254.25604077317 12415.919456019 8842.98070238277 8826.98129271084 10318.9962321025 5182.25453606156 2730.14660272161 18285.0436156491 2215.38537005374 1358.14419366134 316.023775188948 0 76.1813648037587 28983 219900 18 4698.99835801924 4763.00828057879 7858.74704819393 4798.21672251605 12270.719298703 14157.757921468 12579.0716392163 20469.2933829689 33447.593501617 787505.181490201 19642.7638380264 28799.3577077088 16378.2283670686 16074.4002136855 15391.4836767289 18852.1786866538 10495.3007869209 21066.5724417222 7666.22337084627 13287.3642301061 8761.13710428706 8767.01386789976 10876.5598368211 4502.60299686455 3045.90123468678 14574.3263787345 2056.98318609707 1148.16346115286 440.14053097532 0 101.831104128582 29330 207501 19 4769.56305437039 4581.38246080628 8288.32662807874 6428.16399423585 13079.8268829218 13071.1203723884 13681.3457420832 25383.0514374796 23649.7005889726 772568.600575967 19394.6489164952 39066.169617307 12745.0455434697 23989.7203366096 19503.4005511967 8776.24426514558 11745.6028898189 19891.3170503495 7717.15104518644 14612.7991292242 10194.6509337924 9313.89685685857 10482.7479301298 4538.5067749849 2737.8686310539 14505.272430705 2200.48205408151 1381.68220473242 547.462900523905 0 89.7370624589823 29166 225274 20 5601.18218686388 4619.24962818234 7715.87414348023 4858.60275352134 11337.0868606804 17314.5435155503 14436.0109906751 23262.5842955655 24087.9784777294 784522.250191528 18555.3555072353 36013.0668837128 12012.8497920344 20154.6608674201 15237.0900226566 9491.50619508263 11388.1014719851 19957.5937411081 7801.48376663635 10771.5154038862 8509.22069475739 7356.84805694872 10783.1233776761 4544.86908256896 3810.81513046093 16045.1786906355 2048.76512851815 1434.9883929548 420.400708334957 0 99.2550161549625 28828 211853 21 4209.61479932034 4937.84011513992 7171.01677746341 6014.07257196819 11757.1264679013 14640.1767324635 12881.7542503892 25561.7586541094 22680.4895225545 770380.772626394 46770.2179385536 35679.6404626205 12729.2039858082 18835.7254648291 14118.9605374624 27352.1798955421 11463.5116579211 21840.0963005772 6876.55669337314 12599.3744761956 8181.15352407001 7941.93933384345 11010.3717560944 6861.03386278943 5075.18337915061 17565.9283050615 2163.26269989406 1431.26189544171 548.837782637143 0 69.5811619034023 29770 243621 22 4646.58844272244 4891.94793951675 7936.81875197512 4670.82889288403 11350.2548933398 14199.8722760791 14158.7405898192 24835.1943678268 33835.8993715211 751502.991165173 46198.112182081 31902.4625574082 12227.9181646291 20234.907607661 16291.3666794907 8575.25698453721 11871.4617555613 24999.4590169529 6927.58601605845 18590.807278795 9082.72627828778 7119.57929458566 10647.0041616503 4064.20720749268 2978.15373210806 13299.9419848693 2313.19252348725 1313.64702465163 202.731314513586 0 66.8025563842064 28646 214334 23 4881.8682442075 4850.81707460902 7623.71987046428 7211.14300134798 12379.924346262 13435.9052858715 12901.451776912 26357.9440210944 33294.4403194254 853391.812082579 28709.7743253228 36887.0360321868 12581.4798937284 18355.7322286194 13624.0751937164 19204.414748846 12512.3102495583 25641.7342119029 9363.24449198438 11207.9499966251 9088.27075544249 9147.72812989955 12376.0083589123 4187.35354538775 3985.03974933049 16295.6745015299 1795.23020170932 1211.440241419 310.231727613394 0 90.3279810633969 29295 216994 24 4660.80248531401 5258.31449297345 7121.73989902221 7226.36800969806 11631.9560587208 14756.0843009034 14932.5417075482 22912.1847488897 22896.7387563232 760628.135298832 21724.6440989116 34829.1927645772 12733.2583604744 22510.5329778308 17933.4362595372 26976.3720260989 14190.5802054084 22919.1225642217 7010.77375611552 13176.2251744362 8942.81453412439 8486.46608150777 12204.223152892 5323.12223530659 2952.26010494705 15087.6631847882 2014.52935773581 1368.49168182166 346.842447220737 0 92.8677570395894 28575 193410 25 4786.53388424242 4577.12788116278 8800.1052521306 5188.51016021126 11271.0815599133 15092.7432920496 14218.614774348 23640.0566128913 25787.1244938394 764052.89868808 22383.2615990176 36640.1234180982 15381.0456674566 21787.1252866622 15753.0444193497 11088.6785957735 11714.5429667491 20443.0663882958 7057.33211503677 11367.7152803573 8197.49841401463 7846.18539174467 11868.5748268145 5155.0861094242 3889.64764562058 14206.1542010023 2580.17388835634 1268.18110390171 327.885809127786 0 92.1682848974558 29166 211942 26 5401.37678861992 4847.876059065 6963.85988622221 5357.08918146997 12457.2865681355 12298.3552456172 13401.6034115428 24024.423308352 24012.3853277048 741976.24168401 19007.7908711882 36645.2107151724 13789.8269795676 19153.8698868595 11500.0041435386 18629.404719424 15417.6103334604 22537.6023903898 7628.06844455771 15011.8531398114 9498.73726079155 8499.82239147726 10718.8548439877 4923.89632012271 3292.59951676305 14470.7482788197 2608.36378433861 1316.02454773167 274.304131173772 0 92.5690566458166 28632 203712 27 4280.46257532499 4803.1179081288 9147.67203420289 5353.66803679852 13243.3899315404 13356.4146043796 13915.2944709588 22541.3148462342 22794.002318914 781505.876044573 29586.6802620272 36333.8503293161 11582.8645772352 19916.433901032 13597.1923432417 36827.0006723699 13287.6542752179 24016.991444629 8069.20692719479 12868.3247687521 9712.28477085535 6828.00855955181 10853.8423805247 5780.88100596111 4098.38726896676 15586.6555145425 2339.14036168676 1687.97171180552 495.028353568482 0 79.9134451687964 28625 214268 28 4626.90483909723 5113.94917031946 10062.7840145418 5711.66240390116 11262.5412339138 14895.7091052397 14157.318859441 26074.7937028259 22973.3920613003 732517.354827929 19504.0529909517 34191.207059657 12048.3055799596 20014.7476220838 12277.5223082559 10818.3061940754 11978.9229808467 22203.7576302972 7246.39457953605 14018.6571173253 10819.1745106145 7747.21278742543 9099.67139578905 5013.9347269277 2824.4119257711 15919.5025756919 2486.37104136802 1755.48814333111 353.784265736842 0 95.0093128331384 28714 211025 29 4294.16484285718 4568.5078100539 7959.23856079948 4747.90467094145 11989.4609009171 10263.6076880549 13574.7061232509 26405.8625943311 27084.362137832 764281.576956084 38475.7512274326 37023.6156652169 12179.078980124 17233.8999647164 17130.335170362 26877.0421554184 12634.9503502332 22481.2998286639 7812.10069838477 13580.8999793591 8709.35818875408 7932.41006503289 10786.6837256686 5927.38979034974 3223.67395304272 15611.2433285578 2282.41095146121 1552.02662336821 686.589652141391 0 73.8727328595831 29211 212638 30 4444.37125242368 4810.83540018843 7248.25025903232 4879.45946934823 12002.2835145789 13195.9907146102 13274.8508162479 24403.5770466293 26112.4971819343 778696.214124187 18030.5848987007 38266.1687322495 15104.7856301911 18451.4861481825 15122.5499122248 36965.4519921075 14966.3223808613 23392.784841933 8906.240179359 14242.0311483521 10091.9369653951 6401.78849006994 11215.0444128168 3674.50916590665 2765.01499674049 14518.3598056728 1746.98159603015 1205.32143411134 693.434072948835 0 80.2413990843251 28978 206711 31 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11738.4245945145 4298.63464685631 2720.40765742193 14722.4809614678 1944.42366639996 1324.29809491797 452.140647484207 0 90.2724192690799 28735 208153 94 4700.61317314351 5396.75410524934 8514.06668584609 6687.21766154595 13545.9021161028 12443.7873048882 15058.410506948 22894.4905006268 24558.5771581297 781421.01909194 38494.7474578899 32720.6670299467 10693.6264886583 21218.6002860319 14012.8440764272 10222.865672427 13953.0676553007 22615.6342404946 7190.32904507025 13887.3895843199 10213.9718427648 7257.05887390894 10473.6379121402 4642.81092821248 3165.04409739115 13035.8190076827 1822.60787720362 1571.1808704239 237.138982441121 0 85.3232834631146 29109 213563 95 4222.97652481227 4335.51687137952 7702.62618896395 4759.02516565835 12264.7779595315 16474.9650878471 12615.7081279491 20336.2037912585 24092.7994524008 769707.24990323 19372.7984405787 30106.9670634597 13976.3248951503 14803.4591066861 13389.7478367231 17837.2587439653 12133.7112129495 22450.8628710602 8210.23226710258 14413.442542418 9929.29670986039 7937.74605540036 12246.1445706609 4765.21311807442 4025.15137673313 15178.8566063278 2057.77825639696 1649.26147923358 262.471408798559 0 111.187650413473 28674 205729 96 3981.5971432668 4949.14702565289 9343.23976468357 5340.43313533726 11267.9140946475 14611.6771299717 14700.4450747045 24384.3279603219 24506.130681232 733032.590740394 36640.6034326638 37483.2319130317 12320.8745879492 20725.0242662851 15908.1696241592 18534.159905632 13088.9280986024 24622.7284296563 7120.35196785146 14617.9953908147 9208.37122323158 7028.45277124256 10352.2133805488 4722.29293994577 3306.56881822042 15421.2922194097 2454.47218865411 1860.87572496069 393.503275698778 0 76.1701120511794 29157 220531 97 5460.06805368979 4890.30553233751 10187.2736478672 7116.67162486296 13837.8504061774 17341.4801404659 13752.2742233541 25762.1954584428 23473.5472810927 832440.104886984 39485.2339310848 36433.0617724106 13829.3772957454 21097.84707815 17366.5342777053 46191.5958571387 13415.2557135919 19999.496211264 7423.50243237936 12809.3883927422 8426.06169151469 7652.44174531514 12516.1801854711 4598.73988297557 3003.4110487534 18228.0071250559 2147.79064660272 1490.61330196643 782.742771359226 0 72.1476014394868 28745 220872 98 5272.67323668741 5810.81104959426 8582.79843320756 6682.89253229156 11348.6086283247 15512.2838539731 14813.6341194377 24779.8169151543 23679.1919483883 780092.524315736 19530.4726605408 34902.7974256374 13669.2063029444 18815.4605977678 16983.9958139712 9211.73653745912 12633.6971123532 24214.5999691836 8759.27439603545 12984.3668033447 9194.02934006295 7934.07440985947 12916.2434200029 3858.40711591963 2824.77676027209 13169.9595172839 1849.49014615234 1373.90881661794 356.964093391662 0 101.578870297572 28862 211282 99 4513.39094786726 5688.73675712574 8187.05118380141 6707.26179767369 14562.3017725223 14907.774173957 15520.1511957897 23901.3757419631 22721.7908037825 752134.459128546 20805.5725276803 32062.5056701939 12528.5694668127 18892.297866363 13618.4509462043 26592.3990150801 13943.1067273359 21094.6572889844 8662.84329726486 13594.832690851 9311.70116361942 6196.47803692811 10458.9370150552 3890.86696619318 3796.20885225195 13681.8543763625 1862.16654340202 1256.34168148711 508.10390909861 0 101.288111694219 29283 229348 100 5288.9029237506 4576.70994089161 9060.64395703379 7213.13521158653 13779.6243415596 10922.0323263347 15421.9658799226 21616.165862557 32715.858850332 888793.986367762 39696.7751853739 31334.549726654 15045.012232116 16737.0573626676 16885.635098133 17217.0624529156 14388.2718783404 18925.5490787587 6821.71905058308 14144.8163246178 8337.80301816691 7726.25201874515 13705.2818613921 4299.41519585532 2960.20756051708 13988.25603082 2113.44982396292 1363.24040356975 223.729770385792 0 94.8100609616023 28824 208389 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 4758.7714456453 4761.61393901435 8362.85682322858 5804.29772310928 12366.5408463392 14305.5475262267 13780.233263408 23543.9490876291 28356.4972213287 772910.309716773 27765.0875929036 33578.4468674109 13310.4210342356 20122.4155190669 15868.3254586408 20685.6164487767 12983.4180809012 23491.1830841312 7751.24105452436 13088.5761623781 9517.12132933122 7657.84242729147 11090.0084244218 5005.98841046995 3323.03607309781 15299.7950833371 2124.42728229467 1360.7504154321 392.750105669487 0 86.4915788242977 29015.7 216470.33 DEV 458.776541061264 469.514806581003 1021.4082249039 972.578822059305 1086.23199763955 1941.75363896773 1073.42029506686 2334.86181121214 6108.99615821245 46295.2856960193 9386.56376217939 2736.78954184069 1758.2941062406 2399.50002349604 2107.96612871696 10662.2170911674 1229.35145636797 2012.2556034068 689.455694654886 2161.13842305434 774.157248654841 775.557461210112 1290.04768280955 884.895034827193 726.830652997377 1234.86563385147 281.05729379188 198.104198391465 149.770433058149 0 12.7249861398412 281.654948474192 9194.75798491184 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #