---
title: "01-blast"
format: html
editor: visual
---

I am going download blast and use it to compare it unknown sequences.

```{bash}
cd /home/jovyan/applications
curl -O https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ncbi-blast-2.16.0+-x64-linux.tar.gz
tar -xf ncbi-blast-2.16.0+-x64-linux.tar.gz
```

```{bash}
/home/jovyan/applications/ncbi-blast-2.16.0+/bin/blastx -h

```


# make blast database 

I am using swsis-prot uni=prot


```{bash}
cd ../../blastdb
```


```{bash}
cd ../../blastdb

curl -O https://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/complete/uniprot_sprot.fasta.gz

mv uniprot_sprot.fasta.gz uniprot_sprot_r2025_01.fasta.gz

gunzip -k uniprot_sprot_r2025_01.fasta.gz

ls
```
```{bash}
head ../../blastdb/unip*

```




