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"GC":[0.682278,0.510038,0.416138,0.447189,0.405606,0.411893,0.332631,0.407053,0.472134,0.342632,0.500663,0.489858,0.421493,0.420623,0.412286,0.430314,0.441669,0.416797,0.442408,0.414728,0.442672,0.41665,0.422805,0.431966,0.421947,0.436531,0.437907,0.418476,0.44322,0.415481,0.438828,0.415811,0.439143,0.418813,0.423971,0.437641,0.423299,0.437844,0.441464,0.416566,0.442171,0.416562,0.423432,0.41886,0.421011,0.437523,0.428327,0.433532,0.443048,0.418919,0.422456,0.417621,0.44384,0.437731,0.44261,0.438423,0.425998,0.439195,0.430359,0.421796,0.445206,0.42651,0.444327,0.440684,0.435239,0.423385,0.447455,0.427093,0.430196,0.432511,0.442564,0.44035,0.446398,0.444247,0.448033,0.44499,0.450051,0.44426,0.450262,0.445996,0.450234,0.444786,0.450088,0.445289,0.450256,0.447901,0.451125,0.448441,0.455068,0.447263,0.454708,0.450582,0.453397,0.45212,0.455002,0.452292,0.455751,0.453387,0.456546,0.452643,0.461745] }, "kmer_count": { "AAAAA":7581202, "AAAAT":4982050, "AAAAC":3475724, "AAAAG":4415504, 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"ACGTT":942745, "ACGTC":3775642, "ACGTG":845034, "ACGCA":563064, "ACGCT":471648, "ACGCC":289861, "ACGCG":160015, "ACGGA":845228, "ACGGT":671026, "ACGGC":408137, "ACGGG":735427, "AGAAA":6636155, "AGAAT":3276858, "AGAAC":2623191, "AGAAG":5264575, "AGATA":5255559, "AGATT":2136969, "AGATC":6230356, "AGATG":4339054, "AGACA":6092654, "AGACT":2196660, "AGACC":1237073, "AGACG":1019770, "AGAGA":27356071, "AGAGT":4245006, "AGAGC":6901517, "AGAGG":8812175, "AGTAA":3140753, "AGTAT":2906748, "AGTAC":1645824, "AGTAG":5361949, "AGTTA":2408814, "AGTTT":2395806, "AGTTC":1340499, "AGTTG":2293065, "AGTCA":4544245, "AGTCT":1886263, "AGTCC":1133086, "AGTCG":509843, "AGTGA":4466564, "AGTGT":4963127, "AGTGC":1399585, "AGTGG":3928880, "AGCAA":2096342, "AGCAT":2112851, "AGCAC":5251486, "AGCAG":3382256, "AGCTA":1722579, "AGCTT":1416047, "AGCTC":1182409, "AGCTG":2380299, "AGCCA":1653469, "AGCCT":1432891, "AGCCC":643810, "AGCCG":518371, "AGCGA":1293310, "AGCGT":769629, "AGCGC":459750, "AGCGG":696453, "AGGAA":4407699, "AGGAT":2871015, "AGGAC":2256805, "AGGAG":7450034, "AGGTA":3215703, "AGGTT":2428680, "AGGTC":1383127, "AGGTG":3387787, "AGGCA":2182744, "AGGCT":1884117, "AGGCC":1148321, "AGGCG":584405, "AGGGA":5563007, "AGGGT":3918946, "AGGGC":1550174, "AGGGG":5157797, "TAAAA":4427462, "TAAAT":3719718, "TAAAC":2204298, "TAAAG":3085758, "TAATA":3099530, "TAATT":2336835, "TAATC":1295234, "TAATG":3678299, "TAACA":3110757, "TAACT":1699054, "TAACC":979626, "TAACG":665308, "TAAGA":2240932, "TAAGT":1664069, "TAAGC":836739, "TAAGG":2035652, "TATAA":2880637, "TATAT":4249396, "TATAC":2094003, "TATAG":3606799, "TATTA":2825002, "TATTT":3821516, "TATTC":1625400, "TATTG":2664678, "TATCA":1782224, "TATCT":2326040, "TATCC":829382, "TATCG":708153, "TATGA":2588913, "TATGT":3982137, "TATGC":1924116, "TATGG":2609463, "TACAA":2392288, "TACAT":2847758, "TACAC":1802038, "TACAG":4057167, "TACTA":1394610, "TACTT":1624954, "TACTC":959914, "TACTG":2692782, "TACCA":1273689, "TACCT":1262328, "TACCC":646497, "TACCG":415098, "TACGA":510940, "TACGT":621233, "TACGC":260544, "TACGG":560089, "TAGAA":3111160, "TAGAT":3752227, "TAGAC":1811277, "TAGAG":5287791, "TAGTA":4114557, "TAGTT":2296482, "TAGTC":1538941, "TAGTG":3953136, "TAGCA":1949215, "TAGCT":1560631, "TAGCC":1100816, "TAGCG":605610, "TAGGA":2538991, "TAGGT":1947837, "TAGGC":1199820, "TAGGG":3402621, "TTAAA":3755731, "TTAAT":3091294, "TTAAC":1669633, "TTAAG":1719668, "TTATA":2732735, "TTATT":3421048, "TTATC":1224249, "TTATG":2477432, "TTACA":2828199, "TTACT":1726957, "TTACC":936655, "TTACG":462183, "TTAGA":2568928, "TTAGT":2199060, "TTAGC":1160427, "TTAGG":2084336, "TTTAA":3256654, "TTTAT":3205542, "TTTAC":2006115, "TTTAG":2293085, "TTTTA":3335821, "TTTTT":5296708, "TTTTC":2153440, "TTTTG":3076162, "TTTCA":2669755, "TTTCT":2564354, "TTTCC":1549728, "TTTCG":498395, "TTTGA":2943336, "TTTGT":3470604, "TTTGC":1500930, "TTTGG":2598173, "TTCAA":2458757, "TTCAT":2304286, "TTCAC":1468453, "TTCAG":2290865, "TTCTA":1849988, "TTCTT":1940510, "TTCTC":1673331, "TTCTG":2615068, "TTCCA":1798768, "TTCCT":1521170, "TTCCC":864429, "TTCCG":462813, "TTCGA":456945, "TTCGT":476293, "TTCGC":295411, "TTCGG":475592, "TTGAA":3381437, "TTGAT":2603171, "TTGAC":1640170, "TTGAG":2528850, "TTGTA":2812543, "TTGTT":3454432, "TTGTC":1883004, "TTGTG":3612032, "TTGCA":1797297, "TTGCT":1726453, "TTGCC":909067, "TTGCG":482759, "TTGGA":2429925, "TTGGT":2677467, "TTGGC":1432878, "TTGGG":2165791, "TCAAA":3038756, "TCAAT":2195656, "TCAAC":1658229, "TCAAG":1623792, "TCATA":1759700, "TCATT":2567690, "TCATC":1562220, "TCATG":2048120, "TCACA":3819506, "TCACT":1495802, "TCACC":949607, "TCACG":432699, "TCAGA":2894566, "TCAGT":2645944, "TCAGC":1435355, "TCAGG":2063903, "TCTAA":1416969, "TCTAT":2021543, "TCTAC":1283960, "TCTAG":1401256, "TCTTA":1189423, "TCTTT":1898495, "TCTTC":1800221, "TCTTG":1635215, "TCTCA":1717575, "TCTCT":2770209, "TCTCC":2808109, "TCTCG":1334427, "TCTGA":5224045, "TCTGT":3319931, "TCTGC":1728351, "TCTGG":2196325, "TCCAA":1541026, 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"CTTAG":759633, "CTTTA":1767291, "CTTTT":1972775, "CTTTC":1302442, "CTTTG":1775168, "CTTCA":1666861, "CTTCT":1855759, "CTTCC":947620, "CTTCG":408481, "CTTGA":1827911, "CTTGT":1763228, "CTTGC":892674, "CTTGG":1428420, "CTCAA":1655511, "CTCAT":1353058, "CTCAC":926910, "CTCAG":1642336, "CTCTA":1186530, "CTCTT":1289293, "CTCTC":2012576, "CTCTG":2169894, "CTCCA":5110707, "CTCCT":1392412, "CTCCC":780441, "CTCCG":442052, "CTCGA":418133, "CTCGT":1224111, "CTCGC":284972, "CTCGG":433441, "CTGAA":5147560, "CTGAT":1937562, "CTGAC":1694098, "CTGAG":2330110, "CTGTA":3106363, "CTGTT":2756621, "CTGTC":2053267, "CTGTG":3417424, "CTGCA":1953218, "CTGCT":2103728, "CTGCC":1056556, "CTGCG":554053, "CTGGA":2308963, "CTGGT":2362115, "CTGGC":1238470, "CTGGG":2382978, "CCAAA":1921903, "CCAAT":1294039, "CCAAC":991689, "CCAAG":982757, "CCATA":1221227, "CCATT":1555740, "CCATC":1020278, "CCATG":1554614, "CCACA":1623148, "CCACT":1128301, "CCACC":841587, "CCACG":399233, "CCAGA":3345881, "CCAGT":3603032, "CCAGC":1046360, "CCAGG":1587629, "CCTAA":912060, "CCTAT":900965, "CCTAC":772898, "CCTAG":997868, "CCTTA":748958, "CCTTT":1342970, "CCTTC":951042, "CCTTG":1246130, "CCTCA":1111144, "CCTCT":1361173, "CCTCC":1026697, "CCTCG":370312, "CCTGA":1263175, "CCTGT":1888604, "CCTGC":1050676, "CCTGG":1499608, "CCCAA":880650, "CCCAT":866302, "CCCAC":698603, "CCCAG":1101246, "CCCTA":591428, "CCCTT":701409, "CCCTC":738289, "CCCTG":1060292, "CCCCA":693453, "CCCCT":615754, "CCCCC":465515, "CCCCG":285210, "CCCGA":303413, "CCCGT":293840, "CCCGC":205702, "CCCGG":291735, "CCGAA":452164, "CCGAT":459042, "CCGAC":326253, "CCGAG":478145, "CCGTA":410305, "CCGTT":500528, "CCGTC":1033705, "CCGTG":602330, "CCGCA":287773, "CCGCT":311906, "CCGCC":283292, "CCGCG":120892, "CCGGA":338907, "CCGGT":501665, "CCGGC":285775, "CCGGG":470544, "CGAAA":628934, "CGAAT":493327, "CGAAC":336201, "CGAAG":569601, "CGATA":636033, "CGATT":608878, "CGATC":432940, "CGATG":802361, "CGACA":659091, "CGACT":405856, "CGACC":268781, "CGACG":192255, "CGAGA":1392450, "CGAGT":556195, "CGAGC":409671, "CGAGG":793502, "CGTAA":582642, "CGTAT":1287945, "CGTAC":374964, "CGTAG":627178, "CGTTA":599178, "CGTTT":844768, "CGTTC":499735, "CGTTG":760712, "CGTCA":648346, "CGTCT":4289973, "CGTCC":350136, "CGTCG":314872, "CGTGA":782640, "CGTGT":1066733, "CGTGC":416870, "CGTGG":766143, "CGCAA":411085, "CGCAT":351053, "CGCAC":370737, "CGCAG":515283, "CGCTA":337064, "CGCTT":304682, "CGCTC":351905, "CGCTG":604219, "CGCCA":372625, "CGCCT":386453, "CGCCC":202970, "CGCCG":212320, "CGCGA":159607, "CGCGT":346558, "CGCGC":157545, "CGCGG":156833, "CGGAA":5325994, "CGGAT":494870, "CGGAC":373156, "CGGAG":893081, "CGGTA":614902, "CGGTT":591479, "CGGTC":396269, "CGGTG":761978, "CGGCA":535449, "CGGCT":487249, "CGGCC":322143, "CGGCG":282128, "CGGGA":641771, "CGGGT":516384, "CGGGC":351537, "CGGGG":878424, "GAAAA":4505843, "GAAAT":3127913, "GAAAC":2489706, "GAAAG":5249299, "GAATA":3103611, "GAATT":1689574, "GAATC":1226361, "GAATG":3259949, "GAACA":3601103, "GAACT":4067154, "GAACC":825157, "GAACG":805819, "GAAGA":9686900, "GAAGT":2478762, "GAAGC":1567286, "GAAGG":4020295, "GATAA":2447788, "GATAT":4127934, "GATAC":1477083, "GATAG":3859877, "GATTA":1701627, "GATTT":2216463, "GATTC":1024205, "GATTG":1990151, "GATCA":1548405, "GATCT":1327905, "GATCC":749519, "GATCG":5623208, "GATGA":3669776, "GATGT":3255092, "GATGC":1349560, "GATGG":4167434, "GACAA":2620486, "GACAT":2401892, "GACAC":1803336, "GACAG":6303974, "GACTA":1718585, "GACTT":1223144, "GACTC":907018, "GACTG":2790089, "GACCA":1542592, "GACCT":1102275, "GACCC":563499, "GACCG":444056, "GACGA":788591, "GACGT":685666, "GACGC":353646, "GACGG":876762, "GAGAA":7021532, "GAGAT":6192906, "GAGAC":3888813, "GAGAG":25268795, "GAGTA":2736581, "GAGTT":2045182, "GAGTC":1513486, "GAGTG":4516615, "GAGCA":6261433, "GAGCT":1664077, "GAGCC":1013372, "GAGCG":1292432, "GAGGA":7457582, "GAGGT":3647166, "GAGGC":1782676, "GAGGG":6705931, "GTAAA":3201697, "GTAAT":2583701, "GTAAC":1745848, 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"GTGGC":1588613, "GTGGG":3291603, "GCAAA":2151090, "GCAAT":1406636, "GCAAC":1124077, "GCAAG":1382836, "GCATA":1277339, "GCATT":1823676, "GCATC":1081863, "GCATG":1877790, "GCACA":5514872, "GCACT":1244794, "GCACC":701632, "GCACG":486287, "GCAGA":3081191, "GCAGT":2387099, "GCAGC":1624920, "GCAGG":2143861, "GCTAA":1357466, "GCTAT":1447201, "GCTAC":1063521, "GCTAG":1460465, "GCTTA":786999, "GCTTT":1528622, "GCTTC":947069, "GCTTG":1566071, "GCTCA":1270332, "GCTCT":1234354, "GCTCC":770488, "GCTCG":341466, "GCTGA":1952480, "GCTGT":2701075, "GCTGC":1565346, "GCTGG":2081813, "GCCAA":1190086, "GCCAT":1330236, "GCCAC":913205, "GCCAG":1546838, "GCCTA":1020319, "GCCTT":1066044, "GCCTC":820960, "GCCTG":1370496, "GCCCA":760495, "GCCCT":706139, "GCCCC":439403, "GCCCG":259357, "GCCGA":438020, "GCCGT":1166543, "GCCGC":274324, "GCCGG":470252, "GCGAA":471577, "GCGAT":615800, "GCGAC":359043, "GCGAG":1190129, "GCGTA":607940, "GCGTT":615071, "GCGTC":390687, "GCGTG":1046925, "GCGCA":430056, "GCGCT":418271, "GCGCC":296316, "GCGCG":227720, "GCGGA":555624, "GCGGT":616877, "GCGGC":437110, "GCGGG":640932, "GGAAA":4110277, "GGAAT":2335588, "GGAAC":1698521, "GGAAG":8473301, "GGATA":2852766, "GGATT":1634010, "GGATC":1005513, "GGATG":3500753, "GGACA":3190342, "GGACT":1785930, "GGACC":863075, "GGACG":722867, "GGAGA":8701229, "GGAGT":2902130, "GGAGC":1613126, "GGAGG":6486833, "GGTAA":2314308, "GGTAT":2157905, "GGTAC":1437258, "GGTAG":3912143, "GGTTA":3523802, "GGTTT":2318007, "GGTTC":1248512, "GGTTG":2792122, "GGTCA":2166788, "GGTCT":1706228, "GGTCC":927965, "GGTCG":577130, "GGTGA":3135622, "GGTGT":3724022, "GGTGC":1318112, "GGTGG":3993894, "GGCAA":1533315, "GGCAT":1446457, "GGCAC":1066457, "GGCAG":2637583, "GGCTA":1683969, "GGCTT":1250854, "GGCTC":916931, "GGCTG":2437544, "GGCCA":1358635, "GGCCT":1184503, "GGCCC":651897, "GGCCG":472502, "GGCGA":649518, "GGCGT":653677, "GGCGC":356548, "GGCGG":766878, "GGGAA":3409038, "GGGAT":2662060, "GGGAC":1928052, "GGGAG":6027070, "GGGTA":2706187, "GGGTT":3514844, "GGGTC":1616347, "GGGTG":3257884, "GGGCA":1657254, "GGGCT":1792702, "GGGCC":880728, "GGGCG":769793, "GGGGA":4365574, "GGGGT":3782753, "GGGGC":1555861, "GGGGG":16146751 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 22127699, "total_bases": 2234897599, "q20_bases": 2099000159, "q30_bases": 1952947158, "total_cycles": 101, "quality_curves": { 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"T":[35.1749,35.5216,35.338,35.406,35.5056,35.4287,35.4413,35.4239,35.4235,35.4517,35.3328,35.3908,35.4283,35.4091,35.3799,35.3825,35.3347,35.3514,35.3346,35.2523,35.2629,35.2163,35.2091,35.1425,35.1303,35.1315,35.1331,35.0759,35.0676,34.9967,34.9973,34.9499,34.8851,34.9019,34.8122,34.8236,34.7478,34.7129,34.6319,34.5276,34.5469,34.6346,34.6405,34.5404,34.5514,34.4621,34.352,34.3549,34.2107,34.1484,34.2654,34.1694,34.2379,34.3023,34.2953,34.2223,34.2347,34.2683,34.1763,34.2428,34.2402,34.3352,34.3767,34.3859,34.3219,34.2772,34.2136,34.284,34.2083,34.1453,34.0616,34.0484,33.947,33.8977,33.8839,33.8516,33.8025,33.7196,33.7231,33.7965,33.7572,33.7121,33.6002,33.6001,33.6679,33.6413,33.6796,33.6437,33.6452,33.6084,33.6089,33.6305,33.6367,33.6213,33.5838,33.5515,33.5486,33.4974,33.5266,33.5134,33.1193], 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"mean":[34.9179,35.4722,35.5064,35.5974,35.65,35.6285,35.5813,35.5823,35.6088,35.5852,35.5943,35.5651,35.5593,35.5414,35.4931,35.5078,35.4701,35.4905,35.4742,35.3746,35.3922,35.3359,35.3388,35.2589,35.2077,35.1966,35.187,35.1233,35.1198,35.0588,35.0649,35.0411,34.9902,34.9959,34.9546,34.9629,34.9159,34.9055,34.8581,34.8081,34.8186,34.822,34.8141,34.758,34.754,34.7377,34.6913,34.6757,34.5974,34.6061,34.6257,34.6098,34.5706,34.5676,34.5331,34.5172,34.5081,34.5008,34.4833,34.4548,34.429,34.4363,34.4107,34.4098,34.3609,34.3212,34.2994,34.3003,34.2335,34.2216,34.1951,34.1878,34.1378,34.0875,34.0931,34.0743,34.0158,33.9037,33.9332,34.001,33.9753,33.9248,33.8123,33.8285,33.8953,33.862,33.8762,33.8369,33.8223,33.7921,33.7839,33.7904,33.7935,33.7866,33.7527,33.7359,33.7198,33.6849,33.6955,33.698,33.4088] }, "content_curves": { 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"ACGTT":1026974, "ACGTC":688245, "ACGTG":665440, "ACGCA":831682, "ACGCT":775794, "ACGCC":629063, "ACGCG":211304, "ACGGA":598289, "ACGGT":548497, "ACGGC":532641, "ACGGG":331290, "AGAAA":2671952, "AGAAT":1815030, "AGAAC":2000391, "AGAAG":1738663, "AGATA":1623757, "AGATT":1577045, "AGATC":6621722, "AGATG":1828685, "AGACA":3361600, "AGACT":1949905, "AGACC":1701666, "AGACG":643582, "AGAGA":3478898, "AGAGT":3035429, "AGAGC":5012570, "AGAGG":1553300, "AGTAA":1745429, "AGTAT":2049503, "AGTAC":1615101, "AGTAG":1342613, "AGTTA":1722460, "AGTTT":2209412, "AGTTC":1500505, "AGTTG":1504292, "AGTCA":2318632, "AGTCT":2249820, "AGTCC":1712107, "AGTCG":440992, "AGTGA":2710108, "AGTGT":3746818, "AGTGC":1270867, "AGTGG":1275736, "AGCAA":1731829, "AGCAT":1860098, "AGCAC":1745188, "AGCAG":2086948, "AGCTA":1615112, "AGCTT":1515453, "AGCTC":1686096, "AGCTG":2023595, "AGCCA":2180162, "AGCCT":1870259, "AGCCC":1717151, "AGCCG":470305, "AGCGA":443755, "AGCGT":3918814, "AGCGC":451069, "AGCGG":405733, "AGGAA":1648617, "AGGAT":1301142, "AGGAC":1406065, "AGGAG":1662320, "AGGTA":1293085, "AGGTT":1269523, "AGGTC":1170666, "AGGTG":1261344, "AGGCA":1320700, "AGGCT":1530697, "AGGCC":1199467, "AGGCG":430078, "AGGGA":3293050, "AGGGT":946446, "AGGGC":796227, "AGGGG":820701, "TAAAA":3506183, "TAAAT":3003253, "TAAAC":2527083, "TAAAG":1839910, "TAATA":2841801, "TAATT":2498678, "TAATC":1639035, "TAATG":2335807, "TAACA":3400723, "TAACT":2404100, "TAACC":3307656, "TAACG":612855, "TAAGA":1391076, "TAAGT":1203047, "TAAGC":801296, "TAAGG":786734, "TATAA":2764145, "TATAT":4847389, "TATAC":2708187, "TATAG":2134218, "TATTA":3021299, "TATTT":5074627, "TATTC":2790079, "TATTG":2256458, "TATCA":3262893, "TATCT":3810707, "TATCC":2533801, "TATCG":633826, "TATGA":1869390, "TATGT":2763026, "TATGC":2070411, "TATGG":1291214, "TACAA":2783288, "TACAT":3899700, "TACAC":4215502, "TACAG":3245176, "TACTA":3895898, "TACTT":2281906, "TACTC":2576845, "TACTG":3530459, "TACCA":3086211, "TACCT":3005713, "TACCC":2398450, "TACCG":605222, "TACGA":517755, "TACGT":678662, "TACGC":436932, "TACGG":416387, "TAGAA":1854386, "TAGAT":2793893, "TAGAC":1662981, "TAGAG":1338933, "TAGTA":1399841, "TAGTT":1866432, "TAGTC":1677362, "TAGTG":2683609, "TAGCA":1557843, "TAGCT":1719668, "TAGCC":1627731, "TAGCG":357808, "TAGGA":1146060, "TAGGT":983948, "TAGGC":1028426, "TAGGG":2838003, "TTAAA":3295999, "TTAAT":2727939, "TTAAC":2964455, "TTAAG":1274891, "TTATA":2811804, "TTATT":4476653, "TTATC":2364128, "TTATG":2007023, "TTACA":3314229, "TTACT":2925879, "TTACC":2132159, "TTACG":560868, "TTAGA":1714203, "TTAGT":1677412, "TTAGC":1342491, "TTAGG":975559, "TTTAA":3744749, "TTTAT":4369631, "TTTAC":2924306, "TTTAG":2135562, "TTTTA":4308467, "TTTTT":7063281, "TTTTC":3960958, "TTTTG":3846318, "TTTCA":3734323, "TTTCT":5975300, "TTTCC":3601602, "TTTCG":614795, "TTTGA":3020124, "TTTGT":4353503, "TTTGC":2035883, "TTTGG":2181097, "TTCAA":3202534, "TTCAT":3469372, "TTCAC":3080166, "TTCAG":2486335, "TTCTA":3042665, "TTCTT":3848224, 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"CCAGC":2096631, "CCAGG":1648675, "CCTAA":2078604, "CCTAT":2242315, "CCTAC":3245422, "CCTAG":1359904, "CCTTA":1946011, "CCTTT":3236072, "CCTTC":3573028, "CCTTG":1576701, "CCTCA":3513610, "CCTCT":7930520, "CCTCC":5564102, "CCTCG":772874, "CCTGA":2096910, "CCTGT":3428469, "CCTGC":2076146, "CCTGG":1695107, "CCCAA":2305938, "CCCAT":2649233, "CCCAC":3204321, "CCCAG":2854647, "CCCTA":3210242, "CCCTT":2724510, "CCCTC":5584766, "CCCTG":2723541, "CCCCA":3473952, "CCCCT":3973874, "CCCCC":4225899, "CCCCG":993230, "CCCGA":518710, "CCCGT":715972, "CCCGC":752576, "CCCGG":573229, "CCGAA":413154, "CCGAT":538873, "CCGAC":568929, "CCGAG":568320, "CCGTA":653210, "CCGTT":693226, "CCGTC":861810, "CCGTG":549930, "CCGCA":575913, "CCGCT":716766, "CCGCC":837227, "CCGCG":267360, "CCGGA":432512, "CCGGT":504401, "CCGGC":498924, "CCGGG":497074, "CGAAA":520410, "CGAAT":416150, "CGAAC":419329, "CGAAG":340852, "CGATA":506113, "CGATT":519386, "CGATC":491762, "CGATG":533910, "CGACA":779065, "CGACT":550081, "CGACC":651600, "CGACG":266296, "CGAGA":778957, "CGAGT":388984, "CGAGC":415706, "CGAGG":433641, "CGTAA":485707, "CGTAT":588274, "CGTAC":521731, "CGTAG":500101, "CGTTA":722427, "CGTTT":986229, "CGTTC":764592, "CGTTG":661309, "CGTCA":812860, "CGTCT":1025123, "CGTCC":696594, "CGTCG":3369752, "CGTGA":491653, "CGTGT":4017066, "CGTGC":472186, "CGTGG":627753, "CGCAA":526812, "CGCAT":490975, "CGCAC":714747, "CGCAG":631296, "CGCTA":638350, "CGCTT":550422, "CGCTC":1290041, "CGCTG":810726, "CGCCA":729909, "CGCCT":624711, "CGCCC":872183, "CGCCG":437837, "CGCGA":159066, "CGCGT":184458, "CGCGC":342187, "CGCGG":288476, "CGGAA":4679586, "CGGAT":363565, "CGGAC":495493, "CGGAG":542790, "CGGTA":475071, "CGGTT":451546, "CGGTC":506508, "CGGTG":817852, "CGGCA":510972, "CGGCT":520392, "CGGCC":578565, "CGGCG":327553, "CGGGA":410493, "CGGGT":375131, "CGGGC":387617, "CGGGG":753198, "GAAAA":2341458, "GAAAT":2117708, "GAAAC":1807519, "GAAAG":3267599, "GAATA":1651743, "GAATT":1485485, "GAATC":1034398, "GAATG":1568826, "GAACA":2241870, "GAACT":1368966, "GAACC":1270221, "GAACG":569797, "GAAGA":5616225, "GAAGT":1298348, "GAAGC":970830, "GAAGG":1086098, "GATAA":1287763, "GATAT":2459538, "GATAC":1128951, "GATAG":879511, "GATTA":1328420, "GATTT":2022852, "GATTC":1193126, "GATTG":1241614, "GATCA":1404440, "GATCT":1875862, "GATCC":976464, "GATCG":5114005, "GATGA":1662278, "GATGT":1829238, "GATGC":1079539, "GATGG":1163864, "GACAA":1988129, "GACAT":2281423, "GACAC":2437626, "GACAG":2323961, "GACTA":1522135, "GACTT":1427711, "GACTC":1532762, "GACTG":1844936, "GACCA":2063755, "GACCT":1426083, "GACCC":1530191, "GACCG":415730, "GACGA":637858, "GACGT":545478, "GACGC":437816, "GACGG":454122, "GAGAA":1813823, "GAGAT":2240960, "GAGAC":1586203, "GAGAG":2413149, "GAGTA":1027174, "GAGTT":1176092, "GAGTC":979167, "GAGTG":2773732, "GAGCA":1270828, "GAGCT":1227823, "GAGCC":1038862, "GAGCG":3993850, "GAGGA":1552847, "GAGGT":1071754, "GAGGC":903837, "GAGGG":1111392, "GTAAA":2065120, "GTAAT":1748830, 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"GTGGT":1969674, "GTGGC":1003230, "GTGGG":2224437, "GCAAA":1551958, "GCAAT":1269012, "GCAAC":1310833, "GCAAG":1015412, "GCATA":1218072, "GCATT":1772725, "GCATC":1395379, "GCATG":1315069, "GCACA":2143850, "GCACT":1477028, "GCACC":1329156, "GCACG":452746, "GCAGA":1907809, "GCAGT":1452756, "GCAGC":1615332, "GCAGG":1197141, "GCTAA":1249208, "GCTAT":1421103, "GCTAC":1742999, "GCTAG":1024228, "GCTTA":826338, "GCTTT":2046827, "GCTTC":1511920, "GCTTG":949887, "GCTCA":1536126, "GCTCT":2699334, "GCTCC":1564612, "GCTCG":444066, "GCTGA":1512755, "GCTGT":2347348, "GCTGC":1685280, "GCTGG":1223924, "GCCAA":1539161, "GCCAT":1793725, "GCCAC":1653721, "GCCAG":1524608, "GCCTA":1203613, "GCCTT":1547057, "GCCTC":1811468, "GCCTG":1335857, "GCCCA":1568845, "GCCCT":1591383, "GCCCC":1536254, "GCCCG":424994, "GCCGA":421306, "GCCGT":544744, "GCCGC":491357, "GCCGG":444042, "GCGAA":324425, "GCGAT":313916, "GCGAC":350480, "GCGAG":355043, "GCGTA":286169, "GCGTT":491119, "GCGTC":3671270, "GCGTG":1152390, 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"GGGTA":827712, "GGGTT":1114016, "GGGTC":686073, "GGGTG":2223553, "GGGCA":752035, "GGGCT":743253, "GGGCC":771013, "GGGCG":590035, "GGGGA":879549, "GGGGT":2018775, "GGGGC":823065, "GGGGG":26250486 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 21104980, "total_bases": 1681236382, "q20_bases": 1634237817, "q30_bases": 1552163748, "total_cycles": 86, "quality_curves": { 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"T":[35.8523,35.8673,35.8597,35.8368,35.8383,35.8263,35.8198,35.7999,35.7864,35.7556,35.6702,35.6518,35.6845,35.6675,35.6471,35.6288,35.6267,35.5999,35.6123,35.6402,35.6139,35.597,35.5726,35.5697,35.5811,35.5652,35.5379,35.5268,35.5025,35.5097,35.5051,35.4651,35.4792,35.4992,35.4655,35.4584,35.4366,35.398,35.4309,35.4192,35.463,35.3807,35.4092,35.3533,35.3398,35.3974,35.2758,35.2996,35.3223,35.2509,35.2483,35.2927,35.3032,35.2506,35.2729,35.2979,35.2963,35.2799,35.236,35.2279,35.1988,35.196,35.191,35.2004,35.1708,35.1635,35.1005,35.1278,35.1273,35.1101,35.055,35.0329,35.027,35.047,35,35.0384,35.0766,35.065,35.0467,35.0111,34.965,34.9864,34.9471,34.9534,34.9745,34.9179], "C":[36.3614,36.338,36.3235,36.33,36.3127,36.3138,36.2922,36.278,36.2557,36.2447,36.1935,36.2076,36.1904,36.1938,36.1688,36.1586,36.1487,36.1524,36.1501,36.1565,36.1371,36.1384,36.1174,36.1213,36.1136,36.1132,36.093,36.0852,36.0613,36.0683,36.0828,36.0794,36.0116,36.0593,36.0515,36.0599,36.0185,36.0311,35.9811,36.0026,35.9723,35.9901,35.9392,35.9849,35.967,35.8709,35.9443,35.9366,35.8518,35.915,35.9146,35.9203,35.9375,35.8993,35.9088,35.9147,35.8801,35.8744,35.8567,35.8507,35.8274,35.8186,35.8269,35.8237,35.8124,35.7854,35.7779,35.7831,35.7651,35.754,35.7323,35.7161,35.7178,35.7125,35.6951,35.7124,35.7188,35.7002,35.6972,35.6698,35.6545,35.6412,35.6416,35.618,35.6434,35.5547], "G":[35.8985,35.8799,35.8857,35.8986,35.8935,35.8909,35.8725,35.8669,35.8364,35.839,35.7584,35.7557,35.768,35.7663,35.7297,35.7117,35.7222,35.6911,35.708,35.7128,35.6823,35.6837,35.6496,35.6484,35.6598,35.6529,35.6108,35.6071,35.597,35.5552,35.5696,35.5064,35.5119,35.567,35.5236,35.5336,35.4943,35.5271,35.4511,35.4593,35.4934,35.4186,35.4305,35.4362,35.3642,35.3902,35.3987,35.4094,35.3383,35.3666,35.3052,35.4152,35.3814,35.3029,35.334,35.3405,35.3654,35.3564,35.3382,35.305,35.2871,35.2661,35.2724,35.258,35.2468,35.2018,35.1694,35.1908,35.18,35.1565,35.1222,35.0627,35.0725,35.0647,35.0199,35.0317,35.0912,35.0474,35.0483,34.9931,34.9815,34.9655,34.9425,34.9136,34.9505,34.8673], 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"CTTGA":1228035, "CTTGT":1618320, "CTTGC":1007413, "CTTGG":776998, "CTCAA":2056597, "CTCAT":2593399, "CTCAC":3384374, "CTCAG":1881653, "CTCTA":4068387, "CTCTT":4387259, "CTCTC":17914373, "CTCTG":4561725, "CTCCA":3662255, "CTCCT":5294093, "CTCCC":3874962, "CTCCG":656800, "CTCGA":387772, "CTCGT":642767, "CTCGC":851608, "CTCGG":639177, "CTGAA":1881126, "CTGAT":1776362, "CTGAC":2313014, "CTGAG":1396873, "CTGTA":3198479, "CTGTT":3674948, "CTGTC":4740073, "CTGTG":2352441, "CTGCA":2035218, "CTGCT":2553742, "CTGCC":1900487, "CTGCG":408705, "CTGGA":1453955, "CTGGT":1470806, "CTGGC":1188094, "CTGGG":903007, "CCAAA":2099869, "CCAAT":1687133, "CCAAC":2102541, "CCAAG":1193732, "CCATA":2051339, "CCATT":2503903, "CCATC":3126141, "CCATG":1846829, "CCACA":3377528, "CCACT":3101068, "CCACC":2987178, "CCACG":652539, "CCAGA":2096380, "CCAGT":1919780, "CCAGC":1656572, "CCAGG":1308323, "CCTAA":1644088, "CCTAT":1812901, "CCTAC":2593036, "CCTAG":1034318, "CCTTA":1566736, "CCTTT":2651329, "CCTTC":2886767, "CCTTG":1271903, "CCTCA":2760818, "CCTCT":6299752, "CCTCC":4392975, "CCTCG":600161, "CCTGA":1659965, "CCTGT":2719068, "CCTGC":1661734, "CCTGG":1338227, "CCCAA":1742865, "CCCAT":2101617, "CCCAC":2382489, "CCCAG":1991970, "CCCTA":2576020, "CCCTT":2210035, "CCCTC":4428175, "CCCTG":2181408, "CCCCA":2499577, "CCCCT":3133108, "CCCCC":2404177, "CCCCG":648116, "CCCGA":364677, "CCCGT":555748, "CCCGC":478313, "CCCGG":376363, "CCGAA":315464, "CCGAT":423257, "CCGAC":435497, "CCGAG":393385, "CCGTA":506186, "CCGTT":555229, "CCGTC":672789, "CCGTG":429332, "CCGCA":414390, "CCGCT":547992, "CCGCC":572920, "CCGCG":141570, "CCGGA":320239, "CCGGT":382892, "CCGGC":347188, "CCGGG":299794, "CGAAA":404177, "CGAAT":329682, "CGAAC":326676, "CGAAG":262178, "CGATA":399141, "CGATT":410207, "CGATC":377795, "CGATG":421618, "CGACA":586277, "CGACT":424578, "CGACC":491504, "CGACG":195159, "CGAGA":512690, "CGAGT":297030, "CGAGC":294886, "CGAGG":327715, "CGTAA":381325, "CGTAT":457790, "CGTAC":416086, "CGTAG":395201, "CGTTA":571706, "CGTTT":802086, "CGTTC":612517, "CGTTG":519125, "CGTCA":628761, "CGTCT":800810, "CGTCC":546634, "CGTCG":1063207, "CGTGA":376450, "CGTGT":2025947, "CGTGC":368982, "CGTGG":402706, "CGCAA":407157, "CGCAT":388813, "CGCAC":546850, "CGCAG":431380, "CGCTA":489570, "CGCTT":433333, "CGCTC":996249, "CGCTG":634599, "CGCCA":535491, "CGCCT":483307, "CGCCC":598372, "CGCCG":282254, "CGCGA":109923, "CGCGT":132144, "CGCGC":194143, "CGCGG":120110, "CGGAA":1315977, "CGGAT":281649, "CGGAC":351123, "CGGAG":394706, "CGGTA":371778, "CGGTT":351613, "CGGTC":378645, "CGGTG":623071, "CGGCA":372940, "CGGCT":398108, "CGGCC":396696, "CGGCG":175997, "CGGGA":295384, "CGGGT":237097, "CGGGC":230709, "CGGGG":256801, "GAAAA":1892622, "GAAAT":1759300, "GAAAC":1484645, "GAAAG":1988141, "GAATA":1353093, "GAATT":1237451, "GAATC":852452, "GAATG":1293127, "GAACA":1793439, "GAACT":1116895, "GAACC":1025871, "GAACG":451712, "GAAGA":2249704, "GAAGT":1042285, "GAAGC":792190, "GAAGG":873308, "GATAA":1052753, "GATAT":2130061, "GATAC":930840, "GATAG":684252, "GATTA":1096145, "GATTT":1670243, "GATTC":965936, "GATTG":1007540, "GATCA":1136294, "GATCT":1521960, "GATCC":790648, "GATCG":1314344, "GATGA":1366021, "GATGT":1492763, "GATGC":889331, "GATGG":929046, "GACAA":1602494, "GACAT":1872601, "GACAC":1961463, "GACAG":1790595, "GACTA":1239362, "GACTT":1148089, "GACTC":1221293, "GACTG":1480442, "GACCA":1637933, "GACCT":1145136, "GACCC":1221710, "GACCG":327668, "GACGA":477621, "GACGT":429938, "GACGC":332298, "GACGG":350783, "GAGAA":1488658, "GAGAT":1311871, "GAGAC":1297345, "GAGAG":1849616, "GAGTA":831668, "GAGTT":941573, "GAGTC":776842, "GAGTG":1811819, "GAGCA":1020012, "GAGCT":990111, "GAGCC":837330, "GAGCG":1246456, "GAGGA":1252420, "GAGGT":841213, "GAGGC":727085, "GAGGG":740313, "GTAAA":1699018, "GTAAT":1454592, "GTAAC":1192972, "GTAAG":682394, "GTATA":1699179, "GTATT":1978804, "GTATC":1190544, "GTATG":1198973, "GTACA":1823748, "GTACT":1296082, "GTACC":1098941, "GTACG":310942, "GTAGA":1585114, "GTAGT":1945320, "GTAGC":887662, "GTAGG":1500325, "GTTAA":1394916, "GTTAT":1634669, "GTTAC":1369824, "GTTAG":928722, "GTTTA":1807001, "GTTTT":2771467, "GTTTC":1934334, "GTTTG":1751288, "GTTCA":1627479, "GTTCT":2131501, "GTTCC":1310275, "GTTCG":273211, "GTTGA":1403187, "GTTGT":1874099, "GTTGC":929554, "GTTGG":851081, "GTCAA":1381243, "GTCAT":1826919, "GTCAC":1599787, "GTCAG":1443506, "GTCTA":1504006, "GTCTT":1688987, "GTCTC":3033108, "GTCTG":2220276, "GTCCA":1496006, "GTCCT":1774551, "GTCCC":1406646, "GTCCG":296911, "GTCGA":287593, "GTCGT":1270802, "GTCGC":415984, "GTCGG":288240, "GTGAA":1272596, "GTGAT":1979138, "GTGAC":1052152, "GTGAG":856960, "GTGTA":3729686, "GTGTT":2224588, "GTGTC":1501054, "GTGTG":2977420, "GTGCA":1144939, "GTGCT":1110343, "GTGCC":840544, "GTGCG":310347, "GTGGA":1106339, "GTGGT":1241583, "GTGGC":776164, "GTGGG":657796, "GCAAA":1240293, "GCAAT":1029911, "GCAAC":1041064, "GCAAG":782144, "GCATA":985699, "GCATT":1446072, "GCATC":1125401, "GCATG":1064953, "GCACA":1678962, "GCACT":1164207, "GCACC":1057624, "GCACG":349713, "GCAGA":1281949, "GCAGT":1145338, "GCAGC":1292166, "GCAGG":935165, "GCTAA":989044, "GCTAT":1150189, "GCTAC":1389638, "GCTAG":789413, "GCTTA":664675, "GCTTT":1661504, "GCTTC":1204186, "GCTTG":754891, "GCTCA":1211315, "GCTCT":2138168, "GCTCC":1237269, "GCTCG":326327, "GCTGA":1199196, "GCTGT":1863788, "GCTGC":1343579, "GCTGG":938636, "GCCAA":1208982, "GCCAT":1451168, "GCCAC":1297087, "GCCAG":1083388, "GCCTA":965599, "GCCTT":1250785, "GCCTC":1429580, "GCCTG":1057480, "GCCCA":1190336, "GCCCT":1266893, "GCCCC":1101368, "GCCCG":286505, "GCCGA":316160, "GCCGT":408386, "GCCGC":340057, "GCCGG":282730, "GCGAA":253833, "GCGAT":246417, "GCGAC":263633, "GCGAG":250040, "GCGTA":222346, "GCGTT":387451, "GCGTC":1212676, "GCGTG":962140, "GCGCA":312214, "GCGCT":387065, "GCGCC":290467, "GCGCG":138859, "GCGGA":206167, "GCGGT":262463, "GCGGC":251228, "GCGGG":199660, "GGAAA":2203851, "GGAAT":995507, "GGAAC":895463, "GGAAG":1809783, "GGATA":721721, "GGATT":919271, "GGATC":688051, "GGATG":870351, "GGACA":1385834, "GGACT":957257, "GGACC":776389, "GGACG":323076, "GGAGA":1292520, "GGAGT":801347, "GGAGC":732863, "GGAGG":981315, "GGTAA":841443, "GGTAT":864693, "GGTAC":773798, "GGTAG":654035, "GGTTA":843745, "GGTTT":1204018, "GGTTC":696953, "GGTTG":792662, "GGTCA":1117753, "GGTCT":1049129, "GGTCC":700250, "GGTCG":369770, "GGTGA":845475, "GGTGT":1178921, "GGTGC":606823, "GGTGG":965000, "GGCAA":779324, "GGCAT":833182, "GGCAC":741032, "GGCAG":923431, "GGCTA":915139, "GGCTT":821928, "GGCTC":842118, "GGCTG":1041876, "GGCCA":1069052, "GGCCT":962572, "GGCCC":708768, "GGCCG":282861, "GGCGA":256565, "GGCGT":244547, "GGCGC":255003, "GGCGG":263320, "GGGAA":1641780, "GGGAT":636737, "GGGAC":643067, "GGGAG":718088, "GGGTA":603163, "GGGTT":652465, "GGGTC":507478, "GGGTG":640702, "GGGCA":569905, "GGGCT":560449, "GGGCC":553188, "GGGCG":193316, "GGGGA":580958, "GGGGT":521303, "GGGGC":382234, "GGGGG":878760 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213562_sample_1703786_S64_L001_R1_001.fastq.gz --in2 lib_213562_sample_1703786_S64_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213562_sample_1703786_S64_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213562_sample_1703786_S64_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213562_sample_1703786_S64_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213562_sample_1703786_S64_L001_R2_001.fastp-trim.fq.gz " }