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"CATCA":3132944, "CATCT":2787948, "CATCC":1340809, "CATCG":958783, "CATGA":3050789, "CATGT":3443577, "CATGC":1853582, "CATGG":2200766, "CACAA":3624572, "CACAT":3137301, "CACAC":7572306, "CACAG":3510480, "CACTA":1410691, "CACTT":2266198, "CACTC":1359032, "CACTG":2721199, "CACCA":1975411, "CACCT":1458838, "CACCC":757752, "CACCG":701033, "CACGA":731095, "CACGT":3777828, "CACGC":1721403, "CACGG":833243, "CAGAA":4160869, "CAGAT":4593441, "CAGAC":2571984, "CAGAG":5059183, "CAGTA":2701982, "CAGTT":2887198, "CAGTC":3314544, "CAGTG":3666911, "CAGCA":3716538, "CAGCT":2892588, "CAGCC":1568376, "CAGCG":1253203, "CAGGA":3477673, "CAGGT":2475721, "CAGGC":1525543, "CAGGG":2190360, "CTAAA":2853060, "CTAAT":1710781, "CTAAC":1077055, "CTAAG":1167804, "CTATA":1637906, "CTATT":1825236, "CTATC":817097, "CTATG":1398088, "CTACA":2049311, "CTACT":1339062, "CTACC":582724, "CTACG":525884, "CTAGA":2156278, "CTAGT":1182131, "CTAGC":678165, "CTAGG":631599, "CTTAA":2298116, "CTTAT":1666429, "CTTAC":1012337, "CTTAG":1155257, "CTTTA":3075479, "CTTTT":4187037, "CTTTC":1955219, "CTTTG":3123850, "CTTCA":2618689, "CTTCT":2391191, "CTTCC":1252548, "CTTCG":494941, "CTTGA":2264842, "CTTGT":2474814, "CTTGC":1306605, "CTTGG":1738293, "CTCAA":2116376, "CTCAT":2036307, "CTCAC":1326651, "CTCAG":2152058, "CTCTA":1451646, "CTCTT":2126778, "CTCTC":1850544, "CTCTG":2842027, "CTCCA":3625517, "CTCCT":1958458, "CTCCC":938290, "CTCCG":781753, "CTCGA":602765, "CTCGT":973836, "CTCGC":499082, "CTCGG":674333, "CTGAA":5865435, "CTGAT":2907498, "CTGAC":1988117, "CTGAG":3145060, "CTGTA":2903160, "CTGTT":3563770, "CTGTC":2166082, "CTGTG":3735708, "CTGCA":3272297, "CTGCT":3274309, "CTGCC":1367632, "CTGCG":815484, "CTGGA":2912016, "CTGGT":2483971, "CTGGC":1576090, "CTGGG":2105996, "CCAAA":2709968, "CCAAT":1384765, "CCAAC":1295728, "CCAAG":1381958, "CCATA":1333446, "CCATT":1694388, "CCATC":1285139, "CCATG":1734055, "CCACA":2117143, "CCACT":1339067, "CCACC":980031, "CCACG":702647, "CCAGA":2453099, "CCAGT":3010006, 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"CCAGT":2460260, "CCAGC":2581020, "CCAGG":1691892, "CCTAA":1838936, "CCTAT":1349372, "CCTAC":1853662, "CCTAG":739541, "CCTTA":2085541, "CCTTT":4279873, "CCTTC":3697896, "CCTTG":1974909, "CCTCA":3981772, "CCTCT":5462718, "CCTCC":5228568, "CCTCG":1182480, "CCTGA":2638581, "CCTGT":3006227, "CCTGC":2591249, "CCTGG":1769327, "CCCAA":2384353, "CCCAT":2085967, "CCCAC":2470088, "CCCAG":2583733, "CCCTA":1604003, "CCCTT":2753584, "CCCTC":3897724, "CCCTG":2180549, "CCCCA":2842854, "CCCCT":2939229, "CCCCC":4141676, "CCCCG":1201676, "CCCGA":791365, "CCCGT":847692, "CCCGC":940246, "CCCGG":784614, "CCGAA":602378, "CCGAT":640780, "CCGAC":965314, "CCGAG":877713, "CCGTA":622413, "CCGTT":959296, "CCGTC":1349044, "CCGTG":889634, "CCGCA":884744, "CCGCT":1047969, "CCGCC":1212148, "CCGCG":438132, "CCGGA":677029, "CCGGT":601584, "CCGGC":874732, "CCGGG":575842, "CGAAA":817894, "CGAAT":470405, "CGAAC":510674, "CGAAG":541264, "CGATA":645843, "CGATT":667533, "CGATC":567763, "CGATG":840871, "CGACA":1145699, 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"GGGAG":1137313, "GGGTA":654216, "GGGTT":1053163, "GGGTC":735356, "GGGTG":1162633, "GGGCA":931813, "GGGCT":1005274, "GGGCC":941317, "GGGCG":560831, "GGGGA":874177, "GGGGT":877426, "GGGGC":844100, "GGGGG":7125410 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 24824705, "total_bases": 2011895955, "q20_bases": 1954652172, "q30_bases": 1853704556, "total_cycles": 86, "quality_curves": { 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"GC":[0.398382,0.408192,0.405615,0.407066,0.406589,0.411396,0.406207,0.408319,0.404741,0.408167,0.403787,0.406359,0.404753,0.408736,0.404723,0.406592,0.404022,0.406557,0.405662,0.409562,0.405494,0.407585,0.406336,0.408288,0.407919,0.411564,0.407393,0.409712,0.408549,0.406442,0.407145,0.40816,0.404777,0.408682,0.406954,0.410952,0.410265,0.412714,0.409718,0.41422,0.40885,0.408573,0.408824,0.409398,0.406245,0.409032,0.408719,0.409526,0.410254,0.41171,0.408085,0.409895,0.411238,0.409518,0.409121,0.410329,0.407153,0.409805,0.40838,0.408649,0.408609,0.410796,0.408215,0.410701,0.407521,0.40799,0.409412,0.410966,0.405799,0.408776,0.408829,0.408954,0.407341,0.411568,0.406305,0.40899,0.409065,0.409754,0.410993,0.412282,0.409353,0.411852,0.409453,0.409015,0.409897,0.411681] }, "kmer_count": { "AAAAA":15112506, "AAAAT":6840239, "AAAAC":5214473, "AAAAG":6520919, "AAATA":6780706, "AAATT":3468189, "AAATC":2858824, "AAATG":5453525, "AAACA":7039785, "AAACT":3431684, "AAACC":1934373, "AAACG":1299432, 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"AGGCT":1701705, "AGGCC":1134196, "AGGCG":791420, "AGGGA":2934447, "AGGGT":2121340, "AGGGC":1319995, "AGGGG":2658519, "TAAAA":6814925, "TAAAT":4806631, "TAAAC":2880439, "TAAAG":3517611, "TAATA":3546023, "TAATT":2867264, "TAATC":1560029, "TAATG":3119225, "TAACA":2826802, "TAACT":1780836, "TAACC":906175, "TAACG":733303, "TAAGA":2496561, "TAAGT":1789327, "TAAGC":1079323, "TAAGG":1766315, "TATAA":2957857, "TATAT":4001095, "TATAC":1588525, "TATAG":1548246, "TATTA":3101561, "TATTT":4537421, "TATTC":1755861, "TATTG":2258894, "TATCA":2055331, "TATCT":1819202, "TATCC":921638, "TATCG":523250, "TATGA":2262522, "TATGT":3002257, "TATGC":1205148, "TATGG":1499961, "TACAA":2990323, "TACAT":2858200, "TACAC":1769499, "TACAG":2752387, "TACTA":1218477, "TACTT":1943080, "TACTC":987818, "TACTG":2216103, "TACCA":1151917, "TACCT":873744, "TACCC":470751, "TACCG":383647, "TACGA":596422, "TACGT":674848, "TACGC":342833, "TACGG":467900, "TAGAA":2521898, "TAGAT":2132213, "TAGAC":1086057, "TAGAG":2050393, 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"CACAT":2703334, "CACAC":3724221, "CACAG":2925296, "CACTA":1202521, "CACTT":1924906, "CACTC":1192088, "CACTG":2368321, "CACCA":1714252, "CACCT":1273916, "CACCC":679532, "CACCG":612115, "CACGA":594598, "CACGT":783841, "CACGC":558862, "CACGG":698069, "CAGAA":3413487, "CAGAT":2530669, "CAGAC":2190360, "CAGAG":3983676, "CAGTA":2163459, "CAGTT":2396976, "CAGTC":1631607, "CAGTG":3037703, "CAGCA":3153490, "CAGCT":2504317, "CAGCC":1390459, "CAGCG":1062104, "CAGGA":2833387, "CAGGT":2075644, "CAGGC":1317061, "CAGGG":1789409, "CTAAA":2404498, "CTAAT":1436521, "CTAAC":924934, "CTAAG":975102, "CTATA":1321379, "CTATT":1510296, "CTATC":700112, "CTATG":1169880, "CTACA":1725248, "CTACT":1133895, "CTACC":512894, "CTACG":445712, "CTAGA":1002239, "CTAGT":998328, "CTAGC":584998, "CTAGG":537133, "CTTAA":1925969, "CTTAT":1384037, "CTTAC":862574, "CTTAG":973453, "CTTTA":2572403, "CTTTT":3472320, "CTTTC":1679301, "CTTTG":2598486, "CTTCA":2259491, "CTTCT":2046082, "CTTCC":1111790, "CTTCG":406292, 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"GC":[0.41562,0.417138,0.411444,0.415117,0.412519,0.415869,0.416334,0.416596,0.409639,0.415422,0.410269,0.410472,0.411114,0.41204,0.407692,0.412192,0.408503,0.408329,0.411148,0.41104,0.409456,0.412389,0.408336,0.40925,0.410361,0.410926,0.405959,0.41127,0.410076,0.410188,0.410316,0.410026,0.405543,0.411684,0.406081,0.407653,0.408292,0.408832,0.40665,0.410586,0.408451,0.407296,0.408157,0.408295,0.404246,0.410058,0.405106,0.40576,0.407685,0.408518,0.405643,0.4092,0.405675,0.406443,0.407221,0.406714,0.404627,0.407897,0.40513,0.404577,0.406766,0.407209,0.404863,0.407577,0.404959,0.405548,0.40596,0.406889,0.404002,0.407455,0.404397,0.404457,0.40582,0.406728,0.403043,0.408262,0.405628,0.407706,0.406951,0.40776,0.404599,0.408121,0.403714,0.404758,0.406479,0.406363] }, "kmer_count": { "AAAAA":10469531, "AAAAT":5004773, "AAAAC":4184200, "AAAAG":3618963, "AAATA":4753646, "AAATT":3093628, "AAATC":2827344, "AAATG":4140860, "AAACA":5733870, "AAACT":3151755, "AAACC":2814053, "AAACG":1083705, 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"AGGCT":1347058, "AGGCC":1079108, "AGGCG":466579, "AGGGA":1109423, "AGGGT":738958, "AGGGC":791050, "AGGGG":660477, "TAAAA":4836522, "TAAAT":4009808, "TAAAC":2899528, "TAAAG":2619876, "TAATA":3148772, "TAATT":3328481, "TAATC":2060526, "TAATG":2549527, "TAACA":3060036, "TAACT":2151937, "TAACC":1548423, "TAACG":697948, "TAAGA":1648197, "TAAGT":1725445, "TAAGC":1048731, "TAAGG":878658, "TATAA":2820857, "TATAT":4145960, "TATAC":1857735, "TATAG":1341456, "TATTA":3486516, "TATTT":6572184, "TATTC":2438983, "TATTG":2548903, "TATCA":2441263, "TATCT":2846368, "TATCC":1728549, "TATCG":596686, "TATGA":2158186, "TATGT":2705410, "TATGC":1333754, "TATGG":1161258, "TACAA":2894733, "TACAT":3379178, "TACAC":2647896, "TACAG":2464984, "TACTA":1924731, "TACTT":2572242, "TACTC":1610597, "TACTG":2289000, "TACCA":1641544, "TACCT":1675820, "TACCC":1210162, "TACCG":410474, "TACGA":586139, "TACGT":776935, "TACGC":420412, "TACGG":437916, "TAGAA":1779355, "TAGAT":1545769, "TAGAC":1120568, "TAGAG":1278337, 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"CACAT":3812178, "CACAC":7318755, "CACAG":3164570, "CACTA":1950900, "CACTT":2985668, "CACTC":2658435, "CACTG":2971252, "CACCA":3002654, "CACCT":2484657, "CACCC":1906366, "CACCG":974068, "CACGA":633054, "CACGT":883589, "CACGC":959063, "CACGG":667086, "CAGAA":2687054, "CAGAT":2539684, "CAGAC":2447556, "CAGAG":2551697, "CAGTA":2234111, "CAGTT":2699545, "CAGTC":2267393, "CAGTG":2386234, "CAGCA":3519561, "CAGCT":2923392, "CAGCC":2226953, "CAGCG":1025317, "CAGGA":2047389, "CAGGT":1596735, "CAGGC":1348240, "CAGGG":1089639, "CTAAA":2409155, "CTAAT":1973790, "CTAAC":1520671, "CTAAG":981314, "CTATA":1615320, "CTATT":2092922, "CTATC":1685783, "CTATG":1324007, "CTACA":2470821, "CTACT":2196585, "CTACC":1431027, "CTACG":693018, "CTAGA":1009540, "CTAGT":906034, "CTAGC":732248, "CTAGG":442698, "CTTAA":2315904, "CTTAT":2222195, "CTTAC":1558921, "CTTAG":956956, "CTTTA":3404737, "CTTTT":5994534, "CTTTC":3478236, "CTTTG":3212037, "CTTCA":3551537, "CTTCT":4600228, "CTTCC":2892851, "CTTCG":736180, 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"CCTTG":1611299, "CCTCA":3187266, "CCTCT":4452490, "CCTCC":4246545, "CCTCG":948408, "CCTGA":2095412, "CCTGT":2404786, "CCTGC":2079475, "CCTGG":1410018, "CCCAA":1801988, "CCCAT":1679847, "CCCAC":1830866, "CCCAG":1682056, "CCCTA":1331385, "CCCTT":2283831, "CCCTC":3171251, "CCCTG":1763593, "CCCCA":1988465, "CCCCT":2428124, "CCCCC":2606890, "CCCCG":862900, "CCCGA":571126, "CCCGT":667552, "CCCGC":683529, "CCCGG":572591, "CCGAA":475156, "CCGAT":506029, "CCGAC":752153, "CCGAG":596676, "CCGTA":457422, "CCGTT":764745, "CCGTC":1077888, "CCGTG":686554, "CCGCA":645652, "CCGCT":829713, "CCGCC":905330, "CCGCG":311954, "CCGGA":512632, "CCGGT":472391, "CCGGC":645941, "CCGGG":400960, "CGAAA":661035, "CGAAT":379116, "CGAAC":397532, "CGAAG":400091, "CGATA":521189, "CGATT":536953, "CGATC":444180, "CGATG":673265, "CGACA":860480, "CGACT":702502, "CGACC":776910, "CGACG":394999, "CGAGA":604297, "CGAGT":530485, "CGAGC":616545, "CGAGG":544882, "CGTAA":566672, "CGTAT":570482, "CGTAC":574810, "CGTAG":449682, "CGTTA":714753, "CGTTT":1247740, "CGTTC":745184, "CGTTG":665226, "CGTCA":1022804, "CGTCT":1190792, "CGTCC":902640, "CGTCG":419878, "CGTGA":576187, "CGTGT":948258, "CGTGC":527900, "CGTGG":602689, "CGCAA":551085, "CGCAT":614098, "CGCAC":745409, "CGCAG":705482, "CGCTA":458085, "CGCTT":746785, "CGCTC":952457, "CGCTG":1036754, "CGCCA":832045, "CGCCT":790362, "CGCCC":875760, "CGCCG":497942, "CGCGA":174196, "CGCGT":240741, "CGCGC":223274, "CGCGG":275744, "CGGAA":440996, "CGGAT":475140, "CGGAC":565825, "CGGAG":694616, "CGGTA":384017, "CGGTT":550006, "CGGTC":464700, "CGGTG":612989, "CGGCA":676454, "CGGCT":719035, "CGGCC":654121, "CGGCG":397005, "CGGGA":422660, "CGGGT":319373, "CGGGC":346331, "CGGGG":402731, "GAAAA":3641655, "GAAAT":2575795, "GAAAC":2209944, "GAAAG":1792402, "GAATA":1802534, "GAATT":1706266, "GAATC":1197872, "GAATG":1681456, "GAACA":2188134, "GAACT":1368288, "GAACC":1289177, "GAACG":581523, "GAAGA":2004838, "GAAGT":1593709, "GAAGC":1360423, "GAAGG":1153135, "GATAA":1730872, "GATAT":1916758, "GATAC":1068988, "GATAG":736075, "GATTA":1600373, "GATTT":2768727, "GATTC":1276706, "GATTG":1323217, "GATCA":1851021, "GATCT":1327729, "GATCC":1096098, "GATCG":521873, "GATGA":2210795, "GATGT":2137249, "GATGC":1369553, "GATGG":1191757, "GACAA":2177594, "GACAT":2259225, "GACAC":1920318, "GACAG":1925083, "GACTA":1054597, "GACTT":1797463, "GACTC":1478521, "GACTG":1653756, "GACCA":1719698, "GACCT":1400710, "GACCC":1085830, "GACCG":517390, "GACGA":744434, "GACGT":717638, "GACGC":677905, "GACGG":643451, "GAGAA":2107912, "GAGAT":1548172, "GAGAC":1479741, "GAGAG":1756390, "GAGTA":1029189, "GAGTT":1602336, "GAGTC":1121056, "GAGTG":1249006, "GAGCA":1683602, "GAGCT":1603337, "GAGCC":1144197, "GAGCG":584015, "GAGGA":1915372, "GAGGT":1073636, "GAGGC":1080370, "GAGGG":866092, "GTAAA":2495393, "GTAAT":1879340, "GTAAC":1349314, "GTAAG":894398, "GTATA":1607762, "GTATT":2448957, "GTATC":1253150, "GTATG":1343957, "GTACA":2025957, "GTACT":1509967, "GTACC":794989, "GTACG":425581, "GTAGA":1219744, "GTAGT":1209160, "GTAGC":888311, "GTAGG":622057, "GTTAA":2075213, "GTTAT":1952694, "GTTAC":1310020, "GTTAG":955058, "GTTTA":2833313, "GTTTT":4997692, "GTTTC":2565542, "GTTTG":2986756, "GTTCA":2211841, "GTTCT":2046437, "GTTCC":1413767, "GTTCG":414236, "GTTGA":1705450, "GTTGT":2396642, "GTTGC":1304825, "GTTGG":1114393, "GTCAA":1818199, "GTCAT":2183869, "GTCAC":1760454, "GTCAG":1734168, "GTCTA":1080351, "GTCTT":2279417, "GTCTC":2249709, "GTCTG":2176952, "GTCCA":1796785, "GTCCT":2091952, "GTCCC":1234212, "GTCCG":540131, "GTCGA":447603, "GTCGT":639867, "GTCGC":550803, "GTCGG":489773, "GTGAA":1947020, "GTGAT":1717351, "GTGAC":1540652, "GTGAG":1207145, "GTGTA":1741119, "GTGTT":2978910, "GTGTC":1835393, "GTGTG":3750252, "GTGCA":1814290, "GTGCT":1660993, "GTGCC":1074983, "GTGCG":462322, "GTGGA":1435301, "GTGGT":1295214, "GTGGC":1076946, "GTGGG":775029, "GCAAA":2179007, "GCAAT":1339713, "GCAAC":1583137, "GCAAG":1146905, "GCATA":1204832, "GCATT":2086508, "GCATC":1686657, "GCATG":1592980, "GCACA":2290315, "GCACT":1724742, "GCACC":1313067, "GCACG":484136, "GCAGA":2083514, "GCAGT":1711051, "GCAGC":2449252, "GCAGG":1402341, "GCTAA":1359998, "GCTAT":1118018, "GCTAC":1132489, "GCTAG":595969, "GCTTA":1064753, "GCTTT":2906754, "GCTTC":1926825, "GCTTG":1148933, "GCTCA":1978538, "GCTCT":2142153, "GCTCC":1771552, "GCTCG":553502, "GCTGA":1982891, "GCTGT":2468576, "GCTGC":2348976, "GCTGG":1347493, "GCCAA":1619590, "GCCAT":1697770, "GCCAC":1424017, "GCCAG":1351121, "GCCTA":710869, "GCCTT":1703933, "GCCTC":1795219, "GCCTG":1334870, "GCCCA":1212791, "GCCCT":1278864, "GCCCC":1291175, "GCCCG":481129, "GCCGA":515137, "GCCGT":692026, "GCCGC":639428, "GCCGG":482621, "GCGAA":310952, "GCGAT":379222, "GCGAC":518912, "GCGAG":458406, "GCGTA":348727, "GCGTT":688962, "GCGTC":629927, "GCGTG":561670, "GCGCA":451513, "GCGCT":564291, "GCGCC":550982, "GCGCG":185429, "GCGGA":386980, "GCGGT":365606, "GCGGC":489408, "GCGGG":313170, "GGAAA":2058863, "GGAAT":1138669, "GGAAC":1040774, "GGAAG":1163395, "GGATA":919367, "GGATT":1216093, "GGATC":916880, "GGATG":1209256, "GGACA":1647561, "GGACT":1205814, "GGACC":891404, "GGACG":609729, "GGAGA":1627710, "GGAGT":1022808, "GGAGC":1173937, "GGAGG":1347261, "GGTAA":923405, "GGTAT":896491, "GGTAC":613716, "GGTAG":529673, "GGTTA":921642, "GGTTT":1909561, "GGTTC":971202, "GGTTG":998578, "GGTCA":1227006, "GGTCT":1050100, "GGTCC":747031, "GGTCG":363933, "GGTGA":1175103, "GGTGT":1340499, "GGTGC":941189, "GGTGG":867680, "GGCAA":1094883, "GGCAT":1028612, "GGCAC":880196, "GGCAG":1224562, "GGCTA":726141, "GGCTT":1351892, "GGCTC":1189257, "GGCTG":1403072, "GGCCA":1174512, "GGCCT":1088727, "GGCCC":787566, "GGCCG":569893, "GGCGA":399456, "GGCGT":471673, "GGCGC":364352, "GGCGG":421540, "GGGAA":987724, "GGGAT":761640, "GGGAC":756405, "GGGAG":867881, "GGGTA":475770, "GGGTT":793653, "GGGTC":569310, "GGGTG":717091, "GGGCA":725270, "GGGCT":818912, "GGGCC":678239, "GGGCG":326879, "GGGGA":652705, "GGGGT":481047, "GGGGC":552984, "GGGGG":677714 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213561_sample_1703776_S56_L001_R1_001.fastq.gz --in2 lib_213561_sample_1703776_S56_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213561_sample_1703776_S56_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213561_sample_1703776_S56_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213561_sample_1703776_S56_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213561_sample_1703776_S56_L001_R2_001.fastp-trim.fq.gz " }