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"GC":[0.683925,0.512029,0.4267,0.430919,0.390398,0.373583,0.31414,0.398083,0.440761,0.344727,0.474415,0.465995,0.407151,0.402788,0.401157,0.403229,0.414964,0.407572,0.411254,0.411404,0.413652,0.409284,0.413351,0.407651,0.41359,0.411553,0.410965,0.410455,0.416703,0.408572,0.41277,0.40958,0.412379,0.411034,0.417121,0.409542,0.415018,0.413666,0.412766,0.415293,0.41796,0.41127,0.415411,0.415371,0.412254,0.4165,0.418322,0.411139,0.41898,0.415799,0.416717,0.416103,0.421105,0.416946,0.421147,0.417595,0.415159,0.417014,0.419332,0.412521,0.418984,0.417512,0.416707,0.41864,0.421338,0.414168,0.420234,0.421751,0.416592,0.420333,0.421474,0.416246,0.421618,0.420172,0.420308,0.420937,0.425094,0.421587,0.423706,0.423178,0.422542,0.423193,0.426798,0.420354,0.424592,0.427129,0.425026,0.426064,0.430316,0.424669,0.42838,0.428622,0.427349,0.430289,0.433343,0.429393,0.434515,0.433517,0.431878,0.434809,0.438117] }, "kmer_count": { "AAAAA":22404723, "AAAAT":9717806, "AAAAC":7306100, "AAAAG":9422022, 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"ACGTT":1502172, "ACGTC":3757281, "ACGTG":1215845, "ACGCA":948727, "ACGCT":1007126, "ACGCC":660593, "ACGCG":354448, "ACGGA":1233194, "ACGGT":1013828, "ACGGC":1060568, "ACGGG":1007138, "AGAAA":8877260, "AGAAT":3773007, "AGAAC":2977323, "AGAAG":6879994, "AGATA":3576412, "AGATT":3326432, "AGATC":6939918, "AGATG":4521437, "AGACA":4748050, "AGACT":2622118, "AGACC":1422235, "AGACG":1701841, "AGAGA":9506898, "AGAGT":4632590, "AGAGC":6791056, "AGAGG":6135476, "AGTAA":3678757, "AGTAT":2938951, "AGTAC":2143695, "AGTAG":4309120, "AGTTA":3093026, "AGTTT":4378655, "AGTTC":1942269, "AGTTG":4135973, "AGTCA":5376889, "AGTCT":2494339, "AGTCC":1807176, "AGTCG":1090108, "AGTGA":4977485, "AGTGT":4664770, "AGTGC":2365419, "AGTGG":3437321, "AGCAA":3632349, "AGCAT":3036066, "AGCAC":5896019, "AGCAG":5146705, "AGCTA":1882254, "AGCTT":3024471, "AGCTC":2202433, "AGCTG":4348754, "AGCCA":2498157, "AGCCT":1865769, "AGCCC":1236237, "AGCCG":1011229, "AGCGA":1382595, "AGCGT":1278370, "AGCGC":818939, "AGCGG":1432568, "AGGAA":5730942, "AGGAT":3960103, "AGGAC":2901906, "AGGAG":6784071, "AGGTA":2400171, "AGGTT":3207792, "AGGTC":1988597, "AGGTG":3547822, "AGGCA":2910108, "AGGCT":2348235, "AGGCC":1559834, "AGGCG":1164516, "AGGGA":3728593, "AGGGT":2990639, "AGGGC":1797444, "AGGGG":3650250, "TAAAA":9816589, "TAAAT":6432511, "TAAAC":3842667, "TAAAG":4799541, "TAATA":4623545, "TAATT":3766877, "TAATC":2021737, "TAATG":4237796, "TAACA":4040131, "TAACT":2425878, "TAACC":1204130, "TAACG":930329, "TAAGA":3438063, "TAAGT":2550115, "TAAGC":1483365, "TAAGG":2274149, "TATAA":4138126, "TATAT":5276018, "TATAC":2054857, "TATAG":2112528, "TATTA":4173498, "TATTT":6700248, "TATTC":2305198, "TATTG":3172519, "TATCA":2653680, "TATCT":2274805, "TATCC":1250584, "TATCG":713879, "TATGA":3078628, "TATGT":4174339, "TATGC":1668240, "TATGG":2018879, "TACAA":4254438, "TACAT":3761139, "TACAC":2285969, "TACAG":3688901, "TACTA":1678122, "TACTT":2653429, "TACTC":1370482, "TACTG":3086974, "TACCA":1565050, "TACCT":1165619, "TACCC":616156, "TACCG":566629, "TACGA":821625, "TACGT":927812, "TACGC":476839, "TACGG":603837, "TAGAA":3505877, "TAGAT":3309386, "TAGAC":1428357, "TAGAG":2961398, "TAGTA":2822738, "TAGTT":3285415, "TAGTC":2256249, "TAGTG":2975979, "TAGCA":2238656, "TAGCT":1850235, "TAGCC":1019378, "TAGCG":667275, "TAGGA":2290760, "TAGGT":1421855, "TAGGC":1017621, "TAGGG":1614434, "TTAAA":8112785, "TTAAT":4600910, "TTAAC":2975377, "TTAAG":3336353, "TTATA":3750973, "TTATT":5704411, "TTATC":2263599, "TTATG":3111856, "TTACA":4293132, "TTACT":2654629, "TTACC":1226293, "TTACG":771599, "TTAGA":2877652, "TTAGT":2765340, "TTAGC":1762968, "TTAGG":2023821, "TTTAA":7337025, "TTTAT":5673809, "TTTAC":3457162, "TTTAG":3404565, "TTTTA":6703855, "TTTTT":13990410, "TTTTC":5048802, "TTTTG":5793635, "TTTCA":5240287, "TTTCT":4875200, "TTTCC":2743344, "TTTCG":942088, "TTTGA":5214717, "TTTGT":6620655, "TTTGC":3000067, "TTTGG":4347896, "TTCAA":4476473, "TTCAT":4214789, "TTCAC":2604733, "TTCAG":4239846, "TTCTA":2266126, "TTCTT":4321732, "TTCTC":2767149, "TTCTG":3652752, "TTCCA":2616643, "TTCCT":2732674, "TTCCC":1340766, "TTCCG":629644, "TTCGA":710910, "TTCGT":824013, "TTCGC":438177, "TTCGG":704866, "TTGAA":4761888, "TTGAT":4205361, "TTGAC":2648318, "TTGAG":3633443, "TTGTA":4493395, "TTGTT":6736937, "TTGTC":3485000, "TTGTG":5062973, "TTGCA":3359368, "TTGCT":3091076, "TTGCC":1513448, "TTGCG":909144, "TTGGA":3444504, "TTGGT":3759321, "TTGGC":2408784, "TTGGG":2795780, "TCAAA":5676339, "TCAAT":3833039, "TCAAC":2298545, "TCAAG":2371114, "TCATA":2895912, "TCATT":3875720, "TCATC":3038799, "TCATG":3189036, "TCACA":4858251, "TCACT":2458795, "TCACC":1623109, "TCACG":857827, "TCAGA":4081190, "TCAGT":3641501, "TCAGC":2755260, "TCAGG":2949337, "TCTAA":2107093, "TCTAT":2082211, "TCTAC":1553777, "TCTAG":1354999, "TCTTA":2139081, "TCTTT":4430048, "TCTTC":2737665, "TCTTG":3018516, "TCTCA":2517861, "TCTCT":3420519, "TCTCC":2272676, "TCTCG":1115911, "TCTGA":6213933, "TCTGT":4327161, "TCTGC":2819094, "TCTGG":2991526, "TCCAA":2325204, 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"CATTG":2920326, "CATCA":3767388, "CATCT":2970713, "CATCC":1672721, "CATCG":973569, "CATGA":3530866, "CATGT":3863559, "CATGC":1954239, "CATGG":2524979, "CACAA":4117462, "CACAT":3516382, "CACAC":7629744, "CACAG":5215044, "CACTA":1499136, "CACTT":2607231, "CACTC":1539242, "CACTG":3120455, "CACCA":2598037, "CACCT":2044384, "CACCC":1006390, "CACCG":911593, "CACGA":865862, "CACGT":4017284, "CACGC":726099, "CACGG":939606, "CAGAA":4795931, "CAGAT":4932842, "CAGAC":3029633, "CAGAG":6610139, "CAGTA":3091459, "CAGTT":3802232, "CAGTC":3879871, "CAGTG":4211562, "CAGCA":4870297, "CAGCT":3519697, "CAGCC":1941609, "CAGCG":1708916, "CAGGA":4022554, "CAGGT":3034340, "CAGGC":1804373, "CAGGG":2494244, "CTAAA":3173846, "CTAAT":1800902, "CTAAC":1129854, "CTAAG":1207665, "CTATA":1759907, "CTATT":1930698, "CTATC":867979, "CTATG":1528680, "CTACA":2326287, "CTACT":1540256, "CTACC":619011, "CTACG":560515, "CTAGA":1516875, "CTAGT":1341807, "CTAGC":772738, "CTAGG":753596, "CTTAA":2613278, "CTTAT":1888892, 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"CCACG":1665749, "CCAGA":4596167, "CCAGT":2998575, "CCAGC":3688737, "CCAGG":2300265, "CCTAA":2049958, "CCTAT":1397717, "CCTAC":2138938, "CCTAG":860713, "CCTTA":2200202, "CCTTT":4531608, "CCTTC":4100188, "CCTTG":2119784, "CCTCA":4793900, "CCTCT":5659739, "CCTCC":5568618, "CCTCG":1404526, "CCTGA":3160354, "CCTGT":3331196, "CCTGC":3055160, "CCTGG":2334504, "CCCAA":2798679, "CCCAT":2308659, "CCCAC":2767747, "CCCAG":3243859, "CCCTA":1584572, "CCCTT":2807877, "CCCTC":4210095, "CCCTG":2510371, "CCCCA":3286481, "CCCCT":3022583, "CCCCC":4169266, "CCCCG":1358663, "CCCGA":946462, "CCCGT":984153, "CCCGC":1050458, "CCCGG":962355, "CCGAA":729914, "CCGAT":786343, "CCGAC":1158967, "CCGAG":1049763, "CCGTA":714524, "CCGTT":1109577, "CCGTC":1618177, "CCGTG":1046017, "CCGCA":1138576, "CCGCT":1454058, "CCGCC":1409074, "CCGCG":581359, "CCGGA":910155, "CCGGT":764956, "CCGGC":1089331, "CCGGG":712811, "CGAAA":943747, "CGAAT":543598, "CGAAC":619128, "CGAAG":694228, "CGATA":679829, "CGATT":746515, 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"GGCGC":632807, "GGCGG":831705, "GGGAA":3434032, "GGGAT":1121654, "GGGAC":1224541, "GGGAG":1371640, "GGGTA":742975, "GGGTT":1248081, "GGGTC":1155167, "GGGTG":1437176, "GGGCA":1099716, "GGGCT":1250704, "GGGCC":1132255, "GGGCG":664986, "GGGGA":1041441, "GGGGT":945160, "GGGGC":1027325, "GGGGG":7068186 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 28665994, "total_bases": 2337759565, "q20_bases": 2271840867, "q30_bases": 2155485952, "total_cycles": 86, "quality_curves": { 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"CATGG":1847699, "CACAA":4073084, "CACAT":4216382, "CACAC":7925896, "CACAG":3827590, "CACTA":2398410, "CACTT":3581830, "CACTC":2933508, "CACTG":3486861, "CACCA":3715602, "CACCT":2764382, "CACCC":2274680, "CACCG":1377846, "CACGA":1059558, "CACGT":989780, "CACGC":1079270, "CACGG":802347, "CAGAA":3226124, "CAGAT":3278749, "CAGAC":2995328, "CAGAG":3147271, "CAGTA":2580571, "CAGTT":3226849, "CAGTC":2696296, "CAGTG":3327210, "CAGCA":4647465, "CAGCT":3719338, "CAGCC":2696381, "CAGCG":1226692, "CAGGA":2609363, "CAGGT":2228041, "CAGGC":1616078, "CAGGG":1367423, "CTAAA":2754824, "CTAAT":2103316, "CTAAC":1678869, "CTAAG":1105279, "CTATA":1757279, "CTATT":2244729, "CTATC":1632900, "CTATG":1508182, "CTACA":3227917, "CTACT":2687434, "CTACC":2103958, "CTACG":870321, "CTAGA":1153467, "CTAGT":1018927, "CTAGC":874968, "CTAGG":502861, "CTTAA":2671253, "CTTAT":2373956, "CTTAC":1703814, "CTTAG":991342, "CTTTA":3793188, "CTTTT":6779204, "CTTTC":3542475, "CTTTG":3694067, "CTTCA":4242522, "CTTCT":5023925, "CTTCC":3222313, "CTTCG":925789, "CTTGA":1903715, "CTTGT":2628711, "CTTGC":1613155, "CTTGG":1285301, "CTCAA":2870743, "CTCAT":3162651, "CTCAC":3533316, "CTCAG":2889330, "CTCTA":2255936, "CTCTT":4211335, "CTCTC":5320782, "CTCTG":4340638, "CTCCA":3928404, "CTCCT":4922486, "CTCCC":2847385, "CTCCG":1176760, "CTCGA":681696, "CTCGT":947554, "CTCGC":1070937, "CTCGG":945598, "CTGAA":3523054, "CTGAT":2974078, "CTGAC":2493323, "CTGAG":2252194, "CTGTA":3020104, "CTGTT":4481366, "CTGTC":3600866, "CTGTG":3351148, "CTGCA":3731428, "CTGCT":4094914, "CTGCC":2315954, "CTGCG":883914, "CTGGA":2529712, "CTGGT":2330073, "CTGGC":1745057, "CTGGG":1497458, "CCAAA":3606501, "CCAAT":1806163, "CCAAC":2761461, "CCAAG":1881986, "CCATA":1631832, "CCATT":2850185, "CCATC":3063819, "CCATG":2127842, "CCACA":3526907, "CCACT":2799109, "CCACC":3265118, "CCACG":1336636, "CCAGA":2796620, "CCAGT":2407182, "CCAGC":2962470, "CCAGG":1860122, "CCTAA":1639037, "CCTAT":1149383, "CCTAC":1717543, "CCTAG":641627, "CCTTA":1774963, "CCTTT":3724623, "CCTTC":3359803, "CCTTG":1726597, "CCTCA":3862362, "CCTCT":4604529, "CCTCC":4506860, "CCTCG":1118823, "CCTGA":2520075, "CCTGT":2654157, "CCTGC":2457270, "CCTGG":1863866, "CCCAA":2150386, "CCCAT":1856007, "CCCAC":2094142, "CCCAG":2275640, "CCCTA":1303844, "CCCTT":2312263, "CCCTC":3425434, "CCCTG":2031679, "CCCCA":2392075, "CCCCT":2489583, "CCCCC":2701658, "CCCCG":990499, "CCCGA":699952, "CCCGT":775210, "CCCGC":775827, "CCCGG":714429, "CCGAA":581122, "CCGAT":614958, "CCGAC":906981, "CCGAG":749349, "CCGTA":553774, "CCGTT":884246, "CCGTC":1292622, "CCGTG":807888, "CCGCA":870856, "CCGCT":1161804, "CCGCC":1060351, "CCGCG":417536, "CCGGA":705423, "CCGGT":602780, "CCGGC":814703, "CCGGG":515269, "CGAAA":768544, "CGAAT":437430, "CGAAC":482163, "CGAAG":519339, "CGATA":548336, "CGATT":599440, "CGATC":534431, "CGATG":848607, "CGACA":1565272, "CGACT":875208, "CGACC":1109795, "CGACG":653604, "CGAGA":787979, "CGAGT":609307, "CGAGC":792233, "CGAGG":702192, "CGTAA":661213, "CGTAT":648576, "CGTAC":691886, "CGTAG":499817, "CGTTA":779778, "CGTTT":1346925, "CGTTC":861808, "CGTTG":785459, "CGTCA":1247318, "CGTCT":1415042, "CGTCC":1070070, "CGTCG":1349469, "CGTGA":725482, "CGTGT":1763890, "CGTGC":634505, "CGTGG":760963, "CGCAA":715257, "CGCAT":717680, "CGCAC":810302, "CGCAG":926455, "CGCTA":570852, "CGCTT":876876, "CGCTC":1075431, "CGCTG":1437897, "CGCCA":1042014, "CGCCT":979336, "CGCCC":968788, "CGCCG":692232, "CGCGA":240943, "CGCGT":299611, "CGCGC":256351, "CGCGG":434966, "CGGAA":1300957, "CGGAT":571187, "CGGAC":716114, "CGGAG":972493, "CGGTA":493156, "CGGTT":678029, "CGGTC":577507, "CGGTG":981981, "CGGCA":815498, "CGGCT":864302, "CGGCC":868557, "CGGCG":557568, "CGGGA":565623, "CGGGT":533087, "CGGGC":425160, "CGGGG":515236, "GAAAA":4396754, "GAAAT":2961514, "GAAAC":2580227, "GAAAG":2820600, "GAATA":1965981, "GAATT":2011087, "GAATC":1398015, "GAATG":1929167, "GAACA":2598862, "GAACT":1657236, "GAACC":1510162, "GAACG":734331, "GAAGA":3342751, "GAAGT":1968551, "GAAGC":1766388, "GAAGG":1459431, "GATAA":1956900, "GATAT":2027924, "GATAC":1931916, "GATAG":791224, "GATTA":1747000, "GATTT":3093114, "GATTC":1456448, "GATTG":1542342, "GATCA":2271080, "GATCT":1866320, "GATCC":1378264, "GATCG":1413905, "GATGA":2659744, "GATGT":2484463, "GATGC":1624373, "GATGG":1530963, "GACAA":3007059, "GACAT":2691752, "GACAC":2244037, "GACAG":2380989, "GACTA":1242542, "GACTT":2177024, "GACTC":1764206, "GACTG":2065706, "GACCA":2246504, "GACCT":1666454, "GACCC":1316104, "GACCG":702363, "GACGA":986897, "GACGT":886167, "GACGC":827512, "GACGG":933859, "GAGAA":2541725, "GAGAT":2026475, "GAGAC":1842086, "GAGAG":2083646, "GAGTA":1176339, "GAGTT":1855343, "GAGTC":1367184, "GAGTG":2168959, "GAGCA":1996006, "GAGCT":1919770, "GAGCC":1443055, "GAGCG":1485500, "GAGGA":2470193, "GAGGT":1272243, "GAGGC":1402385, "GAGGG":1117532, "GTAAA":2903315, "GTAAT":2111693, "GTAAC":1537695, "GTAAG":1026466, "GTATA":1707786, "GTATT":2725067, "GTATC":1477837, "GTATG":1530829, "GTACA":2333361, "GTACT":1795611, "GTACC":949547, "GTACG":543158, "GTAGA":1734766, "GTAGT":1368638, "GTAGC":1022927, "GTAGG":1381074, "GTTAA":2449434, "GTTAT":2219727, "GTTAC":1496667, "GTTAG":1009740, "GTTTA":3207363, "GTTTT":5776233, "GTTTC":2937726, "GTTTG":3497975, "GTTCA":2581707, "GTTCT":2374590, "GTTCC":1791807, "GTTCG":575545, "GTTGA":1978004, "GTTGT":3001515, "GTTGC":1644445, "GTTGG":1438920, "GTCAA":2270143, "GTCAT":2517108, "GTCAC":2033429, "GTCAG":2066626, "GTCTA":1224358, "GTCTT":2543333, "GTCTC":2405858, "GTCTG":2604516, "GTCCA":2351080, "GTCCT":2314078, "GTCCC":1444580, "GTCCG":670041, "GTCGA":706256, "GTCGT":1494203, "GTCGC":839947, "GTCGG":661392, "GTGAA":2270906, "GTGAT":2728788, "GTGAC":1793247, "GTGAG":1427903, "GTGTA":3085407, "GTGTT":3396813, "GTGTC":2051265, "GTGTG":5786506, "GTGCA":2012572, "GTGCT":2015909, "GTGCC":1614370, "GTGCG":530302, "GTGGA":1863431, "GTGGT":1688222, "GTGGC":1271161, "GTGGG":945050, "GCAAA":2547476, "GCAAT":1499730, "GCAAC":2018209, "GCAAG":1440649, "GCATA":1321080, "GCATT":2306574, "GCATC":1948040, "GCATG":1717858, "GCACA":2427882, "GCACT":1987284, "GCACC":1625728, "GCACG":556328, "GCAGA":2604636, "GCAGT":2057519, "GCAGC":3156479, "GCAGG":1736518, "GCTAA":1509822, "GCTAT":1306356, "GCTAC":1403790, "GCTAG":673016, "GCTTA":1212275, "GCTTT":3232319, "GCTTC":2295695, "GCTTG":1234809, "GCTCA":2242648, "GCTCT":2417252, "GCTCC":2361161, "GCTCG":679174, "GCTGA":2399893, "GCTGT":3070753, "GCTGC":2837541, "GCTGG":2094450, "GCCAA":2014689, "GCCAT":2013013, "GCCAC":1715122, "GCCAG":1921250, "GCCTA":814832, "GCCTT":2078510, "GCCTC":2130388, "GCCTG":1572414, "GCCCA":1498471, "GCCCT":1472239, "GCCCC":1514267, "GCCCG":638914, "GCCGA":647462, "GCCGT":906209, "GCCGC":813958, "GCCGG":601890, "GCGAA":363282, "GCGAT":428373, "GCGAC":678997, "GCGAG":590230, "GCGTA":419843, "GCGTT":763834, "GCGTC":1530218, "GCGTG":644702, "GCGCA":529585, "GCGCT":671153, "GCGCC":672992, "GCGCG":263801, "GCGGA":505082, "GCGGT":477926, "GCGGC":673292, "GCGGG":394105, "GGAAA":3137818, "GGAAT":1354518, "GGAAC":1257114, "GGAAG":2257817, "GGATA":1086407, "GGATT":1367207, "GGATC":1237622, "GGATG":1496108, "GGACA":2100580, "GGACT":1562023, "GGACC":1180223, "GGACG":790997, "GGAGA":2138745, "GGAGT":1276184, "GGAGC":1534301, "GGAGG":1799857, "GGTAA":1108301, "GGTAT":1034551, "GGTAC":755776, "GGTAG":568757, "GGTTA":1053166, "GGTTT":2220180, "GGTTC":1336909, "GGTTG":1119097, "GGTCA":1552294, "GGTCT":1278963, "GGTCC":1110866, "GGTCG":778219, "GGTGA":1440137, "GGTGT":1622931, "GGTGC":1599682, "GGTGG":1286938, "GGCAA":1345007, "GGCAT":1198517, "GGCAC":1028800, "GGCAG":1448398, "GGCTA":881139, "GGCTT":1541839, "GGCTC":1488867, "GGCTG":1744718, "GGCCA":1494598, "GGCCT":1352844, "GGCCC":993545, "GGCCG":758064, "GGCGA":479282, "GGCGT":596264, "GGCGC":471956, "GGCGG":551901, "GGGAA":1948268, "GGGAT":914178, "GGGAC":970153, "GGGAG":1068765, "GGGTA":570121, "GGGTT":967402, "GGGTC":917237, "GGGTG":964371, "GGGCA":862514, "GGGCT":1024946, "GGGCC":839743, "GGGCG":417340, "GGGGA":792240, "GGGGT":565771, "GGGGC":704292, "GGGGG":791914 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213552_sample_1703684_S1_L001_R1_001.fastq.gz --in2 lib_213552_sample_1703684_S1_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213552_sample_1703684_S1_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213552_sample_1703684_S1_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213552_sample_1703684_S1_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213552_sample_1703684_S1_L001_R2_001.fastp-trim.fq.gz " }