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"GC":[0.680672,0.517606,0.422366,0.438839,0.39696,0.402302,0.336763,0.410218,0.465414,0.345119,0.49206,0.481321,0.425069,0.411559,0.416015,0.421501,0.43265,0.419387,0.433773,0.418076,0.433498,0.420756,0.426214,0.423767,0.426009,0.428048,0.429607,0.421735,0.434209,0.419506,0.430715,0.420076,0.430214,0.423327,0.433158,0.428554,0.42695,0.429165,0.428311,0.425248,0.433814,0.42036,0.432636,0.422712,0.424717,0.428858,0.429408,0.423831,0.434032,0.422984,0.426616,0.420982,0.43371,0.428106,0.433227,0.428947,0.430524,0.430013,0.433936,0.424923,0.435686,0.427616,0.43519,0.430909,0.436615,0.426404,0.438624,0.430761,0.433643,0.428076,0.437497,0.43171,0.438317,0.435575,0.439045,0.436588,0.441761,0.435205,0.442441,0.43755,0.441681,0.436344,0.441219,0.437038,0.44294,0.439628,0.443201,0.441191,0.447949,0.440324,0.448487,0.443939,0.446807,0.446176,0.449027,0.446283,0.450839,0.448458,0.452422,0.448388,0.458337] }, "kmer_count": { "AAAAA":7454739, "AAAAT":4892464, "AAAAC":3379429, "AAAAG":4330182, 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"ACGTT":891006, "ACGTC":2809776, "ACGTG":806539, "ACGCA":540523, "ACGCT":452144, "ACGCC":273998, "ACGCG":150492, "ACGGA":817007, "ACGGT":649598, "ACGGC":397357, "ACGGG":698325, "AGAAA":6626476, "AGAAT":3266609, "AGAAC":2614305, "AGAAG":5166293, "AGATA":4052922, "AGATT":2135697, "AGATC":5357651, "AGATG":4363931, "AGACA":6077427, "AGACT":2105390, "AGACC":1904429, "AGACG":1012070, "AGAGA":27925278, "AGAGT":4209673, "AGAGC":5866621, "AGAGG":8765337, "AGTAA":3115118, "AGTAT":2834858, "AGTAC":1628706, "AGTAG":5087395, "AGTTA":2347300, "AGTTT":2372753, "AGTTC":1286245, "AGTTG":2237430, "AGTCA":3739350, "AGTCT":1825323, "AGTCC":1045332, "AGTCG":500037, "AGTGA":4424948, "AGTGT":4830714, "AGTGC":1355982, "AGTGG":3802779, "AGCAA":2037697, "AGCAT":1983460, "AGCAC":4203001, "AGCAG":3339184, "AGCTA":1703319, "AGCTT":1381496, "AGCTC":1129943, "AGCTG":2290629, "AGCCA":1592049, "AGCCT":1392371, "AGCCC":611434, "AGCCG":495448, "AGCGA":1291912, "AGCGT":739130, "AGCGC":435527, "AGCGG":669119, "AGGAA":4326091, "AGGAT":2836843, "AGGAC":2210230, "AGGAG":8273568, "AGGTA":3240456, "AGGTT":2305689, "AGGTC":1314874, "AGGTG":3290601, "AGGCA":2111300, "AGGCT":1845346, "AGGCC":1106065, "AGGCG":552810, "AGGGA":5562222, "AGGGT":3650709, "AGGGC":1506667, "AGGGG":4822063, "TAAAA":4344815, "TAAAT":3608456, "TAAAC":2162557, "TAAAG":3089495, "TAATA":3029755, "TAATT":2255358, "TAATC":1275703, "TAATG":3558379, "TAACA":3029534, "TAACT":1650025, "TAACC":962386, "TAACG":637196, "TAAGA":2197623, "TAAGT":1669683, "TAAGC":812934, "TAAGG":2010139, "TATAA":2764046, "TATAT":4196448, "TATAC":2056335, "TATAG":3390229, "TATTA":2752915, "TATTT":3746678, "TATTC":1583052, "TATTG":2592286, "TATCA":1764421, "TATCT":1470190, "TATCC":798269, "TATCG":483897, "TATGA":2527618, "TATGT":3829538, "TATGC":1305695, "TATGG":2596370, "TACAA":2324870, "TACAT":2793066, "TACAC":1717556, "TACAG":3991376, "TACTA":1364033, "TACTT":1580234, "TACTC":922797, "TACTG":2612812, "TACCA":1238762, "TACCT":1225233, "TACCC":610474, "TACCG":400302, "TACGA":489652, "TACGT":593749, "TACGC":248311, "TACGG":523728, "TAGAA":3034298, "TAGAT":3564906, "TAGAC":1694031, "TAGAG":5267856, "TAGTA":3952749, "TAGTT":2236570, "TAGTC":1470906, "TAGTG":3823773, "TAGCA":1837644, "TAGCT":1518526, "TAGCC":1071085, "TAGCG":562873, "TAGGA":2479263, "TAGGT":1891061, "TAGGC":1158082, "TAGGG":3168158, "TTAAA":3671577, "TTAAT":2916349, "TTAAC":1651499, "TTAAG":1663695, "TTATA":2611586, "TTATT":3349461, "TTATC":1179541, "TTATG":2405291, "TTACA":2743430, "TTACT":1660164, "TTACC":912113, "TTACG":439233, "TTAGA":2485214, "TTAGT":2159571, "TTAGC":1116206, "TTAGG":1999970, "TTTAA":3209297, "TTTAT":3105596, "TTTAC":1946324, "TTTAG":2249446, "TTTTA":3278926, "TTTTT":5183738, "TTTTC":2096807, "TTTTG":2999740, "TTTCA":2597781, "TTTCT":2533090, "TTTCC":1509686, "TTTCG":477644, "TTTGA":2882907, "TTTGT":3374443, "TTTGC":1478105, "TTTGG":2532258, "TTCAA":2384082, "TTCAT":2269131, "TTCAC":1410800, "TTCAG":2225035, "TTCTA":1802494, "TTCTT":1865166, 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"CATCA":2079290, "CATCT":2008178, "CATCC":955252, "CATCG":629701, "CATGA":2071646, "CATGT":2743162, "CATGC":1206226, "CATGG":2141838, "CACAA":2303522, "CACAT":2552342, "CACAC":5956210, "CACAG":3800195, "CACTA":1370698, "CACTT":1359361, "CACTC":993876, "CACTG":2046871, "CACCA":1472853, "CACCT":1069729, "CACCC":639718, "CACCG":440291, "CACGA":510226, "CACGT":3008374, "CACGC":382698, "CACGG":501323, "CAGAA":3303399, "CAGAT":3523469, "CAGAC":2610799, "CAGAG":6002613, "CAGTA":3570478, "CAGTT":2086151, "CAGTC":2894676, "CAGTG":3422573, "CAGCA":2428277, "CAGCT":1890740, "CAGCC":1133620, "CAGCG":722653, "CAGGA":3657876, "CAGGT":2354023, "CAGGC":1206124, "CAGGG":2721918, "CTAAA":1919773, "CTAAT":1379890, "CTAAC":1067256, "CTAAG":924971, "CTATA":2098692, "CTATT":1572813, "CTATC":759518, "CTATG":1604440, "CTACA":1869622, "CTACT":1209826, "CTACC":703107, "CTACG":441953, "CTAGA":1573265, "CTAGT":1716135, "CTAGC":896729, "CTAGG":1177170, "CTTAA":1156129, "CTTAT":1168174, "CTTAC":748990, "CTTAG":756116, "CTTTA":1704505, "CTTTT":1923945, "CTTTC":1257707, "CTTTG":1700414, "CTTCA":1602537, "CTTCT":1454714, "CTTCC":906267, "CTTCG":325964, "CTTGA":1576739, "CTTGT":1649490, "CTTGC":847946, "CTTGG":1346217, "CTCAA":1592114, "CTCAT":1286923, "CTCAC":886184, "CTCAG":1570683, "CTCTA":1145243, "CTCTT":1228804, "CTCTC":1943222, "CTCTG":2080395, "CTCCA":2879069, "CTCCT":1316544, "CTCCC":731311, "CTCCG":418301, "CTCGA":396496, "CTCGT":719998, "CTCGC":271955, "CTCGG":449093, "CTGAA":4207528, "CTGAT":1880708, "CTGAC":1628182, "CTGAG":2222491, "CTGTA":3032515, "CTGTT":2660787, "CTGTC":1985981, "CTGTG":3252701, "CTGCA":1908648, "CTGCT":1794085, "CTGCC":998148, "CTGCG":457888, "CTGGA":2211289, "CTGGT":2257135, "CTGGC":1171530, "CTGGG":2251917, "CCAAA":1868887, "CCAAT":1240293, "CCAAC":957929, "CCAAG":945174, "CCATA":1194798, "CCATT":1505892, "CCATC":958012, "CCATG":1492617, "CCACA":2165546, "CCACT":1088172, "CCACC":795452, "CCACG":378398, "CCAGA":1938139, "CCAGT":2763187, "CCAGC":990183, 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"GGGTT":869962, "GGGTC":640111, "GGGTG":1233570, "GGGCA":701637, "GGGCT":696030, "GGGCC":734143, "GGGCG":524063, "GGGGA":770024, "GGGGT":1087276, "GGGGC":746824, "GGGGG":12951093 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 20240370, "total_bases": 1634847476, "q20_bases": 1587521023, "q30_bases": 1504785404, "total_cycles": 86, "quality_curves": { 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"read2_after_filtering": { "total_reads": 20240370, "total_bases": 1664728184, "q20_bases": 1595842415, "q30_bases": 1490955801, "total_cycles": 86, "quality_curves": { "A":[35.9085,35.8695,35.9068,35.901,35.8378,35.8485,35.814,35.8148,35.7738,35.7318,35.7807,35.7667,35.7427,35.7382,35.685,35.6896,35.6964,35.6733,35.6778,35.6564,35.6731,35.6354,35.6465,35.606,35.5503,35.5717,35.5847,35.5714,35.5155,35.5054,35.5139,35.4824,35.467,35.3859,35.4112,35.4376,35.426,35.374,35.3634,35.3254,35.3177,35.3171,35.3031,35.296,35.2564,35.2152,35.211,35.1731,35.1559,35.1169,35.0932,35.0826,35.095,35.0281,35.0517,35.0436,35.0314,34.991,34.9293,34.9431,34.9295,34.8647,34.7408,34.784,34.8748,34.8471,34.8138,34.673,34.7121,34.7838,34.7698,34.8072,34.753,34.7406,34.6957,34.6912,34.6925,34.6863,34.6547,34.6333,34.5925,34.5668,34.5169,34.5223,34.5177,34.1571], 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"GC":[0.42857,0.430689,0.421973,0.429238,0.42259,0.431472,0.427995,0.430634,0.421011,0.429797,0.42384,0.426054,0.425112,0.429889,0.420801,0.427994,0.421024,0.42714,0.42379,0.42784,0.421671,0.430223,0.422344,0.427869,0.423174,0.429375,0.420544,0.430898,0.423689,0.427847,0.424482,0.42817,0.422334,0.43239,0.42305,0.427792,0.42397,0.429128,0.423044,0.431112,0.424404,0.428628,0.423459,0.430065,0.422201,0.431601,0.423838,0.428197,0.42667,0.431762,0.424111,0.43165,0.42264,0.428358,0.427565,0.431171,0.42414,0.430719,0.421345,0.426003,0.425517,0.428382,0.418428,0.427848,0.419739,0.427515,0.42247,0.42651,0.417946,0.425106,0.420088,0.424809,0.423314,0.425697,0.419894,0.430334,0.422374,0.425782,0.423933,0.429708,0.426305,0.431385,0.422742,0.42798,0.424483,0.429186] }, "kmer_count": { "AAAAA":4212200, "AAAAT":3063000, "AAAAC":2387353, "AAAAG":1712020, "AAATA":3225493, "AAATT":1850412, "AAATC":1754039, "AAATG":2624963, "AAACA":3654463, "AAACT":2011437, "AAACC":1838212, "AAACG":660667, 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"CACAT":3513247, "CACAC":8443920, "CACAG":2781342, "CACTA":3135764, "CACTT":2152581, "CACTC":3449570, "CACTG":2819906, "CACCA":3402537, "CACCT":2561039, "CACCC":2265801, "CACCG":600353, "CACGA":451385, "CACGT":1207668, "CACGC":718918, "CACGG":474327, "CAGAA":1941700, "CAGAT":1968963, "CAGAC":2224120, "CAGAG":1863402, "CAGTA":2155883, "CAGTT":1837864, "CAGTC":2192877, "CAGTG":1744595, "CAGCA":2245262, "CAGCT":1910053, "CAGCC":1842612, "CAGCG":484344, "CAGGA":1415988, "CAGGT":1340574, "CAGGC":1120796, "CAGGG":913602, "CTAAA":1798113, "CTAAT":1602137, "CTAAC":2201107, "CTAAG":648804, "CTATA":2734853, "CTATT":2335155, "CTATC":2810661, "CTATG":1407727, "CTACA":3373083, "CTACT":3884362, "CTACC":2871016, "CTACG":480096, "CTAGA":1157028, "CTAGT":1291529, "CTAGC":1152068, "CTAGG":792881, "CTTAA":1347503, "CTTAT":1675534, "CTTAC":1503356, "CTTAG":752306, "CTTTA":2423764, "CTTTT":2994526, "CTTTC":3560321, "CTTTG":1952858, "CTTCA":2625880, "CTTCT":3697282, "CTTCC":2647228, "CTTCG":428934, 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"CCTTG":1228852, "CCTCA":2708533, "CCTCT":6248802, "CCTCC":4300286, "CCTCG":593605, "CCTGA":1592183, "CCTGT":2667052, "CCTGC":1623785, "CCTGG":1272021, "CCCAA":1689627, "CCCAT":2061789, "CCCAC":2379138, "CCCAG":1870307, "CCCTA":2400445, "CCCTT":2144433, "CCCTC":4372386, "CCCTG":2091700, "CCCCA":2430626, "CCCCT":3028831, "CCCCC":2297273, "CCCCG":624147, "CCCGA":346729, "CCCGT":534723, "CCCGC":451432, "CCCGG":355785, "CCGAA":304361, "CCGAT":411977, "CCGAC":420296, "CCGAG":364332, "CCGTA":456186, "CCGTT":543509, "CCGTC":660908, "CCGTG":411108, "CCGCA":391926, "CCGCT":531916, "CCGCC":545840, "CCGCG":133004, "CCGGA":297738, "CCGGT":368648, "CCGGC":328718, "CCGGG":287112, "CGAAA":390068, "CGAAT":322669, "CGAAC":318685, "CGAAG":253311, "CGATA":384591, "CGATT":399623, "CGATC":369706, "CGATG":403980, "CGACA":562144, "CGACT":419010, "CGACC":471544, "CGACG":185114, "CGAGA":469500, "CGAGT":279198, "CGAGC":279712, "CGAGG":305075, "CGTAA":364808, "CGTAT":428413, "CGTAC":395468, "CGTAG":859641, "CGTTA":546781, "CGTTT":780990, "CGTTC":597220, "CGTTG":502458, "CGTCA":614575, "CGTCT":795206, "CGTCC":529448, "CGTCG":769789, "CGTGA":360240, "CGTGT":1152057, "CGTGC":356502, "CGTGG":365834, "CGCAA":391664, "CGCAT":372051, "CGCAC":516156, "CGCAG":408399, "CGCTA":457890, "CGCTT":418197, "CGCTC":1007129, "CGCTG":607706, "CGCCA":513417, "CGCCT":456272, "CGCCC":576634, "CGCCG":230545, "CGCGA":103734, "CGCGT":124662, "CGCGC":182889, "CGCGG":114246, "CGGAA":1013818, "CGGAT":264301, "CGGAC":324760, "CGGAG":403479, "CGGTA":357141, "CGGTT":343143, "CGGTC":366764, "CGGTG":518666, "CGGCA":354669, "CGGCT":382941, "CGGCC":374487, "CGGCG":164483, "CGGGA":278475, "CGGGT":213461, "CGGGC":218882, "CGGGG":247010, "GAAAA":1850239, "GAAAT":1716373, "GAAAC":1484820, "GAAAG":1690908, "GAATA":1316611, "GAATT":1176075, "GAATC":893498, "GAATG":1245613, "GAACA":1746024, "GAACT":1075782, "GAACC":972693, "GAACG":432959, "GAAGA":1891126, "GAAGT":1012815, "GAAGC":761978, "GAAGG":829649, "GATAA":1018777, "GATAT":1262914, "GATAC":910758, "GATAG":678915, "GATTA":1081757, "GATTT":1626312, "GATTC":920523, "GATTG":978563, "GATCA":1109980, "GATCT":1342903, "GATCC":764914, "GATCG":1019854, "GATGA":1322730, "GATGT":1450843, "GATGC":843418, "GATGG":879434, "GACAA":1551089, "GACAT":1813739, "GACAC":1823672, "GACAG":1726261, "GACTA":1189949, "GACTT":1121918, "GACTC":1179210, "GACTG":1434030, "GACCA":1586616, "GACCT":1095065, "GACCC":1172867, "GACCG":309688, "GACGA":450222, "GACGT":414719, "GACGC":317683, "GACGG":333219, "GAGAA":1418123, "GAGAT":1206310, "GAGAC":1236847, "GAGAG":1789632, "GAGTA":800819, "GAGTT":917783, "GAGTC":747519, "GAGTG":1495600, "GAGCA":978006, "GAGCT":945729, "GAGCC":804115, "GAGCG":934655, "GAGGA":1185730, "GAGGT":827665, "GAGGC":702407, "GAGGG":687621, "GTAAA":1657307, "GTAAT":1415643, "GTAAC":1151365, "GTAAG":653785, "GTATA":1658921, "GTATT":1934065, "GTATC":1154601, "GTATG":1167848, "GTACA":1739530, "GTACT":1280945, "GTACC":1074430, "GTACG":299415, "GTAGA":1390791, "GTAGT":1047080, "GTAGC":863863, "GTAGG":1662636, "GTTAA":1389752, "GTTAT":1583981, "GTTAC":1909563, "GTTAG":893979, "GTTTA":1769521, "GTTTT":2698674, "GTTTC":1895673, "GTTTG":1703659, "GTTCA":1596675, "GTTCT":2127211, "GTTCC":1273785, "GTTCG":260352, "GTTGA":1291056, "GTTGT":1832894, "GTTGC":901856, "GTTGG":823728, "GTCAA":1350443, "GTCAT":1788313, "GTCAC":1553431, "GTCAG":1392923, "GTCTA":1412118, "GTCTT":1711007, "GTCTC":2995961, "GTCTG":2198191, "GTCCA":1451873, "GTCCT":1734131, "GTCCC":1368581, "GTCCG":284578, "GTCGA":279972, "GTCGT":989902, "GTCGC":359834, "GTCGG":269625, "GTGAA":1226849, "GTGAT":1083664, "GTGAC":1020719, "GTGAG":811502, "GTGTA":2355196, "GTGTT":2743561, "GTGTC":1432250, "GTGTG":2731306, "GTGCA":1105986, "GTGCT":1081350, "GTGCC":809248, "GTGCG":291539, "GTGGA":1041565, "GTGGT":1128657, "GTGGC":740966, "GTGGG":617450, "GCAAA":1209852, "GCAAT":1002745, "GCAAC":1006481, "GCAAG":742245, "GCATA":955385, "GCATT":1406687, "GCATC":1107657, "GCATG":1026349, "GCACA":1607831, "GCACT":1134930, "GCACC":1022999, "GCACG":334300, "GCAGA":1244123, "GCAGT":1107126, "GCAGC":1240045, "GCAGG":888173, "GCTAA":957158, "GCTAT":1143257, "GCTAC":1277958, "GCTAG":773341, "GCTTA":650554, "GCTTT":1628710, "GCTTC":1176480, "GCTTG":742667, "GCTCA":1166101, "GCTCT":2139433, "GCTCC":1195098, "GCTCG":320919, "GCTGA":1169963, "GCTGT":1806854, "GCTGC":1293413, "GCTGG":891513, "GCCAA":1161335, "GCCAT":1402773, "GCCAC":1256891, "GCCAG":1019023, "GCCTA":935511, "GCCTT":1213939, "GCCTC":1394785, "GCCTG":1015862, "GCCCA":1141357, "GCCCT":1230766, "GCCCC":1058450, "GCCCG":265778, "GCCGA":299784, "GCCGT":367816, "GCCGC":325155, "GCCGG":265258, "GCGAA":245890, "GCGAT":234221, "GCGAC":251807, "GCGAG":235652, "GCGTA":213389, "GCGTT":374929, "GCGTC":911163, "GCGTG":323575, "GCGCA":295519, "GCGCT":368099, "GCGCC":275172, "GCGCG":130312, "GCGGA":194630, "GCGGT":252683, "GCGGC":236849, "GCGGG":180089, "GGAAA":1909157, "GGAAT":955307, "GGAAC":851903, "GGAAG":1483271, "GGATA":699694, "GGATT":892012, "GGATC":662487, "GGATG":834743, "GGACA":1324767, "GGACT":887483, "GGACC":731609, "GGACG":302021, "GGAGA":1230273, "GGAGT":770897, "GGAGC":687806, "GGAGG":938271, "GGTAA":820033, "GGTAT":853357, "GGTAC":722916, "GGTAG":618247, "GGTTA":832304, "GGTTT":1169005, "GGTTC":679140, "GGTTG":763049, "GGTCA":1078400, "GGTCT":1006778, "GGTCC":670615, "GGTCG":302970, "GGTGA":811389, "GGTGT":1509260, "GGTGC":578616, "GGTGG":827238, "GGCAA":748681, "GGCAT":819262, "GGCAC":712324, "GGCAG":876596, "GGCTA":894553, "GGCTT":804903, "GGCTC":806914, "GGCTG":1002238, "GGCCA":1012210, "GGCCT":928706, "GGCCC":676530, "GGCCG":268800, "GGCGA":243921, "GGCGT":229319, "GGCGC":234810, "GGCGG":248417, "GGGAA":1348542, "GGGAT":614210, "GGGAC":595245, "GGGAG":679400, "GGGTA":558399, "GGGTT":629486, "GGGTC":486819, "GGGTG":591993, "GGGCA":540267, "GGGCT":534453, "GGGCC":525210, "GGGCG":183806, "GGGGA":545884, "GGGGT":484247, "GGGGC":369958, "GGGGG":602797 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213537_sample_1703774_S54_L001_R1_001.fastq.gz --in2 lib_213537_sample_1703774_S54_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213537_sample_1703774_S54_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213537_sample_1703774_S54_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213537_sample_1703774_S54_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213537_sample_1703774_S54_L001_R2_001.fastp-trim.fq.gz " }