fastp report

Summary

General

fastp version: 0.23.4 (https://github.com/OpenGene/fastp)

sequencing: paired end (101 cycles + 101 cycles)

mean length before filtering: 101bp, 101bp

mean length after filtering: 79bp, 81bp

duplication rate: 13.551791%

Insert size peak: 116

Detected read1 adapter: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Before filtering

total reads: 38.464554 M

total bases: 3.884920 G

Q20 bases: 3.710736 G (95.516413%)

Q30 bases: 3.486999 G (89.757294%)

GC content: 43.818374%

After filtering

total reads: 37.059714 M

total bases: 2.994592 G

Q20 bases: 2.890382 G (96.520051%)

Q30 bases: 2.722049 G (90.898813%)

GC content: 42.511113%

Filtering result

reads passed filters: 37.059714 M (96.347702%)

reads with low quality: 1.219744 M (3.171086%)

reads with too many N: 7.524000 K (0.019561%)

reads too short: 177.572000 K (0.461651%)

Adapters

Adapter or bad ligation of read1

Sequence Occurrences

A 123105

AG 121701

AGA 126736

AGAT 128966

AGATC 152964

AGATCG 145103

AGATCGG 139373

AGATCGGA 136318

AGATCGGAA 133372

AGATCGGAAG 128037

AGATCGGAAGA 125068

AGATCGGAAGAG 124607

AGATCGGAAGAGC 122696

AGATCGGAAGAGCA 122950

AGATCGGAAGAGCAC 121009

AGATCGGAAGAGCACA 120395

AGATCGGAAGAGCACAC 117904

AGATCGGAAGAGCACACG 112913

AGATCGGAAGAGCACACGT 106865

AGATCGGAAGAGCACACGTC 104595

AGATCGGAAGAGCACACGTCT 99696

AGATCGGAAGAGCACACGTCTG 96872

AGATCGGAAGAGCACACGTCTGA 94247

AGATCGGAAGAGCACACGTCTGAA 93895

AGATCGGAAGAGCACACGTCTGAAC 90764

AGATCGGAAGAGCACACGTCTGAACT 89355

AGATCGGAAGAGCACACGTCTGAACTC 85660

AGATCGGAAGAGCACACGTCTGAACTCC 83278

AGATCGGAAGAGCACACGTCTGAACTCCA 78812

AGATCGGAAGAGCACACGTCTGAACTCCAG 74572

AGATCGGAAGAGCACACGTCTGAACTCCAGT 69116

AGATCGGAAGAGCACACGTCTGAACTCCAGTC 66957

AGATCGGAAGAGCACACGTCTGAACTCCAGTCA 63528

AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 61239

AGATCGGAAGAGCACACGTCTGAACTCCAGTCACT 59072

AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT 57097

AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC 55950

AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC 52955

other adapter sequences 1315284

Adapter or bad ligation of read2

Sequence Occurrences

A 122413

AG 120789

AGA 125953

AGAT 127941

AGATC 130734

AGATCG 133108

AGATCGG 131715

AGATCGGA 130371

AGATCGGAA 127333

AGATCGGAAG 123220

AGATCGGAAGA 120583

AGATCGGAAGAG 120188

AGATCGGAAGAGC 118666

AGATCGGAAGAGCG 118474

AGATCGGAAGAGCGT 114681

AGATCGGAAGAGCGTC 115496

AGATCGGAAGAGCGTCG 112785

AGATCGGAAGAGCGTCGT 104262

AGATCGGAAGAGCGTCGTG 101846

AGATCGGAAGAGCGTCGTGT 95886

AGATCGGAAGAGCGTCGTGTA 90540

AGATCGGAAGAGCGTCGTGTAG 88700

AGATCGGAAGAGCGTCGTGTAGG 85807

AGATCGGAAGAGCGTCGTGTAGGG 85365

AGATCGGAAGAGCGTCGTGTAGGGA 82594

AGATCGGAAGAGCGTCGTGTAGGGAA 81681

AGATCGGAAGAGCGTCGTGTAGGGAAA 77719

AGATCGGAAGAGCGTCGTGTAGGGAAAG 75405

AGATCGGAAGAGCGTCGTGTAGGGAAAGA 71326

AGATCGGAAGAGCGTCGTGTAGGGAAAGAG 67360

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGT 62992

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG 60474

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT 57430

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC 55615

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCG 53578

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGC 51963

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCA 50621

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCAC 47904

AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCACA 45592

other adapter sequences 692729

Insert size estimation

This estimation is based on paired-end overlap analysis, and there are 26.815784% reads found not overlapped.
The nonoverlapped read pairs may have insert size <30 or >172, or contain too much sequencing errors to be detected as overlapped.

Before filtering

Before filtering: read1: quality

Value of each position will be shown on mouse over.

Before filtering: read1: base contents

Value of each position will be shown on mouse over.

Before filtering: read1: KMER counting

Darker background means larger counts. The count will be shown on mouse over.

AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG

AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG

AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG

AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG

AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG

ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG

ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG

ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG

ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG

ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG

ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG

ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG

ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG

AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG

AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG

AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG

AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG

TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG

TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG

TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG

TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG

TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG

TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG

TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG

TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG

TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG

TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG

TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG

TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG

TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG

TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG

TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG

TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG

CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG

CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG

CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG

CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG

CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG

CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG

CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG

CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG

CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG

CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG

CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG

CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG

CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG

CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG

CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG

CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG

GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG

GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG

GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG

GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG

GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG

GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG

GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG

GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG

GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG

GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG

GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG

GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG

GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG

GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG

GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG

GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG

Before filtering: read2: quality

Value of each position will be shown on mouse over.

Before filtering: read2: base contents

Value of each position will be shown on mouse over.

Before filtering: read2: KMER counting

Darker background means larger counts. The count will be shown on mouse over.

AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG

AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG

AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG

AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG

AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG

ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG

ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG

ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG

ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG

ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG

ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG

ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG

ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG

AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG

AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG

AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG

AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG

TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG

TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG

TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG

TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG

TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG

TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG

TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG

TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG

TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG

TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG

TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG

TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG

TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG

TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG

TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG

TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG

CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG

CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG

CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG

CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG

CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG

CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG

CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG

CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG

CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG

CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG

CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG

CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG

CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG

CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG

CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG

CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG

GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG

GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG

GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG

GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG

GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG

GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG

GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG

GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG

GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG

GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG

GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG

GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG

GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG

GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG

GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG

GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG

After filtering

After filtering: read1: quality

Value of each position will be shown on mouse over.

After filtering: read1: base contents

Value of each position will be shown on mouse over.

After filtering: read1: KMER counting

Darker background means larger counts. The count will be shown on mouse over.

AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG

AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG

AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG

AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG

AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG

ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG

ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG

ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG

ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG

ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG

ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG

ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG

ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG

AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG

AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG

AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG

AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG

TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG

TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG

TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG

TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG

TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG

TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG

TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG

TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG

TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG

TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG

TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG

TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG

TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG

TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG

TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG

TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG

CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG

CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG

CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG

CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG

CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG

CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG

CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG

CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG

CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG

CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG

CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG

CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG

CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG

CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG

CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG

CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG

GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG

GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG

GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG

GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG

GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG

GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG

GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG

GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG

GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG

GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG

GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG

GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG

GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG

GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG

GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG

GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG

After filtering: read2: quality

Value of each position will be shown on mouse over.

After filtering: read2: base contents

Value of each position will be shown on mouse over.

After filtering: read2: KMER counting

Darker background means larger counts. The count will be shown on mouse over.

AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG

AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG

AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG

AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG

AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG

ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG

ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG

ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG

ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG

ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG

ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG

ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG

ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG

AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG

AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG

AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG

AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG

TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG

TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG

TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG

TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG

TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG

TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG

TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG

TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG

TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG

TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG

TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG

TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG

TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG

TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG

TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG

TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG

CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG

CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG

CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG

CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG

CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG

CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG

CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG

CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG

CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG

CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG

CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG

CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG

CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG

CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG

CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG

CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG

GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG

GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG

GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG

GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG

GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG

GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG

GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG

GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG

GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG

GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG

GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG

GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG

GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG

GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG

GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG

GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG

fastp --in1 lib_213527_sample_1703791_S67_L001_R1_001.fastq.gz --in2 lib_213527_sample_1703791_S67_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213527_sample_1703791_S67_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213527_sample_1703791_S67_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213527_sample_1703791_S67_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213527_sample_1703791_S67_L001_R2_001.fastp-trim.fq.gz

fastp 0.23.4, at 2026-08-08 11:04:57