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"CCAGT":2527603, "CCAGC":2694517, "CCAGG":1812093, "CCTAA":1826707, "CCTAT":1326367, "CCTAC":1864410, "CCTAG":738795, "CCTTA":1980429, "CCTTT":4139811, "CCTTC":3621767, "CCTTG":1985438, "CCTCA":3903080, "CCTCT":5230271, "CCTCC":5125620, "CCTCG":1216094, "CCTGA":2721365, "CCTGT":3041839, "CCTGC":2702712, "CCTGG":1904325, "CCCAA":2412113, "CCCAT":2069598, "CCCAC":2474261, "CCCAG":2597185, "CCCTA":1588312, "CCCTT":2668495, "CCCTC":3764483, "CCCTG":2262026, "CCCCA":2831839, "CCCCT":2903051, "CCCCC":4071950, "CCCCG":1214255, "CCCGA":796961, "CCCGT":855422, "CCCGC":944733, "CCCGG":824172, "CCGAA":620939, "CCGAT":665898, "CCGAC":989059, "CCGAG":922792, "CCGTA":654828, "CCGTT":972166, "CCGTC":1401348, "CCGTG":935105, "CCGCA":919982, "CCGCT":1081399, "CCGCC":1245552, "CCGCG":451965, "CCGGA":734770, "CCGGT":642476, "CCGGC":928295, "CCGGG":612140, "CGAAA":843646, "CGAAT":475211, "CGAAC":523621, "CGAAG":566461, "CGATA":667760, "CGATT":670335, "CGATC":579417, "CGATG":888251, "CGACA":1173557, 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"GTGGA":2003928, "GTGGT":2030470, "GTGGC":1456018, "GTGGG":1688024, "GCAAA":2764997, "GCAAT":1667167, "GCAAC":2024137, "GCAAG":1521787, "GCATA":1448438, "GCATT":2530047, "GCATC":2105446, "GCATG":1998382, "GCACA":2916534, "GCACT":2155287, "GCACC":1702908, "GCACG":671657, "GCAGA":3100564, "GCAGT":2218619, "GCAGC":3247275, "GCAGG":1876294, "GCTAA":1700289, "GCTAT":1383586, "GCTAC":1422977, "GCTAG":808622, "GCTTA":1319708, "GCTTT":3552153, "GCTTC":2458144, "GCTTG":1456237, "GCTCA":2540442, "GCTCT":2685396, "GCTCC":2283684, "GCTCG":727883, "GCTGA":2626711, "GCTGT":3152225, "GCTGC":3043014, "GCTGG":1822521, "GCCAA":2141593, "GCCAT":2181796, "GCCAC":1874907, "GCCAG":2005728, "GCCTA":973257, "GCCTT":2175695, "GCCTC":2293633, "GCCTG":1791481, "GCCCA":1664493, "GCCCT":1668652, "GCCCC":1759048, "GCCCG":661317, "GCCGA":740126, "GCCGT":1045440, "GCCGC":909069, "GCCGG":731743, "GCGAA":430088, "GCGAT":484935, "GCGAC":692695, "GCGAG":664497, "GCGTA":452980, "GCGTT":891067, "GCGTC":3610680, 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"GGGAG":1281397, "GGGTA":671410, "GGGTT":1173428, "GGGTC":774955, "GGGTG":2068060, "GGGCA":1027968, "GGGCT":1085376, "GGGCC":1017175, "GGGCG":591525, "GGGGA":988716, "GGGGT":1237670, "GGGGC":918142, "GGGGG":12099780 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 24914165, "total_bases": 2031950150, "q20_bases": 1979423696, "q30_bases": 1888154115, "total_cycles": 86, "quality_curves": { 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"T":[36.0036,35.9933,36.0036,35.995,35.9898,35.9833,35.962,35.9415,35.9222,35.9099,35.8357,35.8026,35.82,35.8259,35.8072,35.7949,35.791,35.7801,35.7682,35.7805,35.7481,35.7416,35.7296,35.7245,35.7232,35.7078,35.7064,35.6905,35.6663,35.6746,35.6674,35.6479,35.6485,35.6616,35.6271,35.6181,35.6159,35.5738,35.5977,35.5696,35.6223,35.548,35.5885,35.5799,35.5509,35.5993,35.5337,35.5009,35.5361,35.4853,35.447,35.4219,35.4618,35.45,35.4655,35.4913,35.4864,35.4711,35.4442,35.4132,35.3727,35.3892,35.3728,35.3814,35.3769,35.3441,35.2923,35.3332,35.3301,35.3142,35.2792,35.267,35.2593,35.2886,35.2286,35.2678,35.2764,35.2695,35.2393,35.2203,35.1805,35.1948,35.1581,35.1538,35.1235,35.1484], "C":[36.3882,36.4058,36.3801,36.3626,36.3565,36.3503,36.3297,36.3309,36.3037,36.297,36.2461,36.2483,36.253,36.229,36.2157,36.1964,36.1928,36.1908,36.1891,36.1703,36.1652,36.1723,36.1566,36.143,36.1201,36.1129,36.0882,36.0844,36.0796,36.0767,36.0957,36.0773,36.0527,36.0539,36.0562,36.0494,36.0091,36.018,35.9961,36.0033,35.9576,35.9709,35.9766,35.9606,35.949,35.9398,35.9092,35.9104,35.8805,35.8819,35.8358,35.8596,35.8575,35.8673,35.8687,35.8596,35.8353,35.8251,35.7956,35.7696,35.7643,35.7413,35.7414,35.7522,35.746,35.7069,35.7037,35.7006,35.6771,35.6579,35.6384,35.6276,35.6241,35.6368,35.5988,35.6077,35.61,35.5912,35.5802,35.5578,35.5468,35.5256,35.5372,35.4901,35.517,35.3858], "G":[36.0137,36.0281,36.0198,36.0126,36.0108,36.0114,35.9926,35.9895,35.9651,35.9642,35.8742,35.8638,35.8759,35.8582,35.8406,35.8323,35.842,35.8296,35.8109,35.8066,35.7791,35.7764,35.7665,35.7511,35.7558,35.7495,35.7089,35.6892,35.684,35.6752,35.6933,35.6087,35.6274,35.6645,35.6116,35.6201,35.6075,35.6394,35.5735,35.549,35.5843,35.5395,35.5658,35.5652,35.4776,35.5335,35.5296,35.5305,35.477,35.4946,35.4176,35.5033,35.4738,35.4258,35.4251,35.4442,35.4663,35.4542,35.4368,35.3807,35.3816,35.361,35.386,35.37,35.3547,35.3137,35.286,35.3121,35.288,35.2328,35.2165,35.1799,35.2042,35.2146,35.1789,35.1498,35.2039,35.1756,35.1768,35.1431,35.1,35.0878,35.0987,35.0484,35.0565,34.9298], 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"CCTTG":1612782, "CCTCA":3135481, "CCTCT":4261727, "CCTCC":4157835, "CCTCG":974739, "CCTGA":2176204, "CCTGT":2436682, "CCTGC":2175714, "CCTGG":1523114, "CCCAA":1830397, "CCCAT":1666301, "CCCAC":1842901, "CCCAG":1817746, "CCCTA":1318358, "CCCTT":2207006, "CCCTC":3056708, "CCCTG":1831752, "CCCCA":2029324, "CCCCT":2391451, "CCCCC":2540788, "CCCCG":874343, "CCCGA":590423, "CCCGT":674972, "CCCGC":692435, "CCCGG":607095, "CCGAA":492894, "CCGAT":527217, "CCGAC":767775, "CCGAG":664636, "CCGTA":509226, "CCGTT":773832, "CCGTC":1120061, "CCGTG":722568, "CCGCA":690920, "CCGCT":858431, "CCGCC":933362, "CCGCG":325909, "CCGGA":566223, "CCGGT":505393, "CCGGC":689596, "CCGGG":430144, "CGAAA":686646, "CGAAT":383649, "CGAAC":408856, "CGAAG":426682, "CGATA":540303, "CGATT":540943, "CGATC":455747, "CGATG":712567, "CGACA":894708, "CGACT":711998, "CGACC":812848, "CGACG":427800, "CGAGA":689658, "CGAGT":571179, "CGAGC":663786, "CGAGG":600993, "CGTAA":579226, "CGTAT":606856, "CGTAC":598461, "CGTAG":470795, "CGTTA":715189, "CGTTT":1241539, "CGTTC":757711, "CGTTG":690865, "CGTCA":1066375, "CGTCT":1214764, "CGTCC":922246, "CGTCG":1129329, "CGTGA":607990, "CGTGT":1630784, "CGTGC":558569, "CGTGG":689244, "CGCAA":571465, "CGCAT":618048, "CGCAC":769117, "CGCAG":766249, "CGCTA":477340, "CGCTT":749528, "CGCTC":962402, "CGCTG":1092515, "CGCCA":878004, "CGCCT":821805, "CGCCC":898886, "CGCCG":616206, "CGCGA":190559, "CGCGT":248221, "CGCGC":229370, "CGCGG":302207, "CGGAA":1163746, "CGGAT":509960, "CGGAC":601731, "CGGAG":767858, "CGGTA":414546, "CGGTT":580106, "CGGTC":490832, "CGGTG":850493, "CGGCA":729314, "CGGCT":758920, "CGGCC":696152, "CGGCG":434724, "CGGGA":467029, "CGGGT":349963, "CGGGC":376607, "CGGGG":461326, "GAAAA":3746261, "GAAAT":2637326, "GAAAC":2291127, "GAAAG":2562993, "GAATA":1822586, "GAATT":1722500, "GAATC":1218277, "GAATG":1743555, "GAACA":2246964, "GAACT":1409313, "GAACC":1337487, "GAACG":621840, "GAAGA":2865324, "GAAGT":1664331, "GAAGC":1447874, "GAAGG":1263166, "GATAA":1770216, "GATAT":1917790, "GATAC":1081390, "GATAG":773571, "GATTA":1617405, "GATTT":2759509, "GATTC":1286767, "GATTG":1361100, "GATCA":1890655, "GATCT":1634841, "GATCC":1131626, "GATCG":1246045, "GATGA":2317890, "GATGT":2206820, "GATGC":1423999, "GATGG":1284785, "GACAA":2262133, "GACAT":2294131, "GACAC":1952952, "GACAG":2047367, "GACTA":1079480, "GACTT":1845547, "GACTC":1512221, "GACTG":2333251, "GACCA":1790442, "GACCT":1473500, "GACCC":1111921, "GACCG":553121, "GACGA":797533, "GACGT":749196, "GACGC":709807, "GACGG":720511, "GAGAA":2229377, "GAGAT":1773886, "GAGAC":1563649, "GAGAG":1946074, "GAGTA":1065184, "GAGTT":1663332, "GAGTC":1182449, "GAGTG":1997532, "GAGCA":1787172, "GAGCT":1689790, "GAGCC":1207660, "GAGCG":1340290, "GAGGA":2114566, "GAGGT":1157662, "GAGGC":1171357, "GAGGG":1011387, "GTAAA":2516218, "GTAAT":1868700, "GTAAC":1368367, "GTAAG":923770, "GTATA":1598697, "GTATT":2421495, "GTATC":1278130, "GTATG":1383833, "GTACA":2040474, "GTACT":1527266, "GTACC":807574, "GTACG":446569, "GTAGA":1617072, "GTAGT":1225471, "GTAGC":915922, "GTAGG":1300798, "GTTAA":2071733, "GTTAT":1959491, "GTTAC":1309092, "GTTAG":978613, "GTTTA":2841832, "GTTTT":4985897, "GTTTC":2542216, "GTTTG":3057532, "GTTCA":2244015, "GTTCT":2040461, "GTTCC":1427935, "GTTCG":436414, "GTTGA":1754909, "GTTGT":2427024, "GTTGC":1329492, "GTTGG":1181388, "GTCAA":1874125, "GTCAT":2192525, "GTCAC":1796039, "GTCAG":1810220, "GTCTA":1093972, "GTCTT":2263368, "GTCTC":2227197, "GTCTG":2257842, "GTCCA":1839739, "GTCCT":2059817, "GTCCC":1259993, "GTCCG":569353, "GTCGA":475157, "GTCGT":1336714, "GTCGC":674026, "GTCGG":540869, "GTGAA":2035093, "GTGAT":1773184, "GTGAC":2263521, "GTGAG":1300790, "GTGTA":2835494, "GTGTT":3029943, "GTGTC":1900902, "GTGTG":6089096, "GTGCA":1865258, "GTGCT":1703318, "GTGCC":1121424, "GTGCG":497828, "GTGGA":1578942, "GTGGT":1522382, "GTGGC":1159728, "GTGGG":868416, "GCAAA":2220667, "GCAAT":1354280, "GCAAC":1627147, "GCAAG":1194736, "GCATA":1190024, "GCATT":2083804, "GCATC":1716919, "GCATG":1632431, "GCACA":2329777, "GCACT":1746353, "GCACC":1367385, "GCACG":513955, "GCAGA":2252605, "GCAGT":1795276, "GCAGC":2581321, "GCAGG":1516928, "GCTAA":1385718, "GCTAT":1126836, "GCTAC":1150856, "GCTAG":616998, "GCTTA":1066334, "GCTTT":2893650, "GCTTC":1963592, "GCTTG":1175108, "GCTCA":2030119, "GCTCT":2161642, "GCTCC":1806478, "GCTCG":576388, "GCTGA":2096340, "GCTGT":2535124, "GCTGC":2459348, "GCTGG":1458427, "GCCAA":1695334, "GCCAT":1727627, "GCCAC":1475457, "GCCAG":1477595, "GCCTA":734553, "GCCTT":1747758, "GCCTC":1845625, "GCCTG":1430966, "GCCCA":1283114, "GCCCT":1328729, "GCCCC":1310186, "GCCCG":505391, "GCCGA":564084, "GCCGT":785794, "GCCGC":687582, "GCCGG":541420, "GCGAA":326530, "GCGAT":393606, "GCGAC":544101, "GCGAG":509031, "GCGTA":364972, "GCGTT":710706, "GCGTC":1342458, "GCGTG":613827, "GCGCA":478401, "GCGCT":591469, "GCGCC":582968, "GCGCG":201029, "GCGGA":424746, "GCGGT":398135, "GCGGC":530170, "GCGGG":342458, "GGAAA":2848312, "GGAAT":1195355, "GGAAC":1110724, "GGAAG":1959348, "GGATA":964209, "GGATT":1258110, "GGATC":976916, "GGATG":1301785, "GGACA":1758408, "GGACT":1276149, "GGACC":953718, "GGACG":670163, "GGAGA":1813087, "GGAGT":1096273, "GGAGC":1288571, "GGAGG":1549125, "GGTAA":968476, "GGTAT":915583, "GGTAC":636797, "GGTAG":561339, "GGTTA":961546, "GGTTT":1963659, "GGTTC":1005860, "GGTTG":1062626, "GGTCA":1301962, "GGTCT":1091081, "GGTCC":791019, "GGTCG":505100, "GGTGA":1270029, "GGTGT":1880682, "GGTGC":990639, "GGTGG":1132141, "GGCAA":1162270, "GGCAT":1075458, "GGCAC":930407, "GGCAG":1340043, "GGCTA":751400, "GGCTT":1391333, "GGCTC":1260209, "GGCTG":1519025, "GGCCA":1266535, "GGCCT":1166261, "GGCCC":838468, "GGCCG":622661, "GGCGA":434422, "GGCGT":509814, "GGCGC":389927, "GGCGG":459053, "GGGAA":1749371, "GGGAT":819000, "GGGAC":830899, "GGGAG":978320, "GGGTA":509214, "GGGTT":857610, "GGGTC":611726, "GGGTG":1274110, "GGGCA":803486, "GGGCT":885000, "GGGCC":741240, "GGGCG":357027, "GGGGA":736888, "GGGGT":555474, "GGGGC":611331, "GGGGG":804779 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213523_sample_1703756_S135_L002_R1_001.fastq.gz --in2 lib_213523_sample_1703756_S135_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213523_sample_1703756_S135_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213523_sample_1703756_S135_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213523_sample_1703756_S135_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213523_sample_1703756_S135_L002_R2_001.fastp-trim.fq.gz " }