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"CGAAT":973515, "CGAAC":697485, "CGAAG":1119664, "CGATA":1283846, "CGATT":1242130, "CGATC":987259, "CGATG":1687324, "CGACA":1397562, "CGACT":851613, "CGACC":566815, "CGACG":386978, "CGAGA":6004829, "CGAGT":1058476, "CGAGC":956675, "CGAGG":1488100, "CGTAA":1114979, "CGTAT":1590582, "CGTAC":773373, "CGTAG":1449761, "CGTTA":1152077, "CGTTT":2109303, "CGTTC":1051502, "CGTTG":1715156, "CGTCA":1348239, "CGTCT":7598388, "CGTCC":769075, "CGTCG":774137, "CGTGA":1391776, "CGTGT":2765390, "CGTGC":911272, "CGTGG":1802650, "CGCAA":807131, "CGCAT":735114, "CGCAC":869771, "CGCAG":1030014, "CGCTA":662318, "CGCTT":635047, "CGCTC":781921, "CGCTG":1250243, "CGCCA":766121, "CGCCT":798984, "CGCCC":452527, "CGCCG":460657, "CGCGA":327555, "CGCGT":688524, "CGCGC":364458, "CGCGG":534665, "CGGAA":10141106, "CGGAT":1139554, "CGGAC":815581, "CGGAG":1721077, "CGGTA":1261498, "CGGTT":1434561, "CGGTC":885670, "CGGTG":1766833, "CGGCA":1060085, "CGGCT":958600, "CGGCC":730262, "CGGCG":726369, "CGGGA":1288413, 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"GGCTC":1868804, "GGCTG":4689600, "GGCCA":2748451, "GGCCT":2548380, "GGCCC":1336514, "GGCCG":990274, "GGCGA":1225898, "GGCGT":1621330, "GGCGC":744477, "GGCGG":1790614, "GGGAA":6372874, "GGGAT":4735067, "GGGAC":3424626, "GGGAG":12306408, "GGGTA":6045209, "GGGTT":7314836, "GGGTC":3096274, "GGGTG":6798216, "GGGCA":3464654, "GGGCT":3307616, "GGGCC":1823953, "GGGCG":1541940, "GGGGA":7728396, "GGGGT":8409805, "GGGGC":3110252, "GGGGG":21874992 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 46835047, "total_bases": 4730339747, "q20_bases": 4516566258, "q30_bases": 4263781502, "total_cycles": 101, "quality_curves": { 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"CTAGA":3823180, "CTAGT":3414526, "CTAGC":2901119, "CTAGG":1988248, "CTTAA":3528034, "CTTAT":4244040, "CTTAC":3720531, "CTTAG":2063025, "CTTTA":5677685, "CTTTT":7380978, "CTTTC":8554233, "CTTTG":5011641, "CTTCA":7243296, "CTTCT":9609084, "CTTCC":6415892, "CTTCG":1185520, "CTTGA":3254314, "CTTGT":4641397, "CTTGC":2535535, "CTTGG":2074956, "CTCAA":5917504, "CTCAT":6431629, "CTCAC":8763396, "CTCAG":5200909, "CTCTA":10107749, "CTCTT":10168972, "CTCTC":45587948, "CTCTG":11738726, "CTCCA":10087640, "CTCCT":12789756, "CTCCC":10336004, "CTCCG":1626814, "CTCGA":1000592, "CTCGT":1561033, "CTCGC":2428342, "CTCGG":1975717, "CTGAA":5094681, "CTGAT":4571106, "CTGAC":6148122, "CTGAG":3790440, "CTGTA":7620826, "CTGTT":8320973, "CTGTC":12506620, "CTGTG":6207724, "CTGCA":8163545, "CTGCT":9075084, "CTGCC":5171775, "CTGCG":1052094, "CTGGA":4775967, "CTGGT":4685671, "CTGGC":3126596, "CTGGG":2622598, "CCAAA":5785478, "CCAAT":4321233, "CCAAC":7614753, "CCAAG":3492257, "CCATA":5327683, "CCATT":5684924, "CCATC":7177077, "CCATG":4432917, "CCACA":14844985, "CCACT":8644862, "CCACC":7861508, "CCACG":1841483, "CCAGA":7172791, "CCAGT":4762919, "CCAGC":4409211, "CCAGG":3443227, "CCTAA":4175149, "CCTAT":3917616, "CCTAC":8027430, "CCTAG":2801582, "CCTTA":3954760, "CCTTT":6063231, "CCTTC":6571124, "CCTTG":3162980, "CCTCA":7330307, "CCTCT":13783919, "CCTCC":9887205, "CCTCG":1441600, "CCTGA":4379976, "CCTGT":6694571, "CCTGC":4411563, "CCTGG":3645134, "CCCAA":5727234, "CCCAT":4842872, "CCCAC":7862148, "CCCAG":5433909, "CCCTA":6425385, "CCCTT":4954074, "CCCTC":9558289, "CCCTG":5347712, "CCCCA":7283685, "CCCCT":7460368, "CCCCC":7767762, "CCCCG":1877936, "CCCGA":1062020, "CCCGT":1332065, "CCCGC":1452557, "CCCGG":1085288, "CCGAA":855879, "CCGAT":1131766, "CCGAC":1232620, "CCGAG":1182239, "CCGTA":1433804, "CCGTT":1347648, "CCGTC":1631425, "CCGTG":1184557, "CCGCA":1258159, "CCGCT":1553637, "CCGCC":1671114, "CCGCG":510021, "CCGGA":870715, "CCGGT":962475, "CCGGC":1006127, "CCGGG":958497, "CGAAA":1082074, "CGAAT":823148, "CGAAC":878451, "CGAAG":733433, "CGATA":1010886, "CGATT":1082264, "CGATC":986077, "CGATG":1093912, "CGACA":2020118, "CGACT":1127900, "CGACC":1434991, "CGACG":598116, "CGAGA":1631758, "CGAGT":759900, "CGAGC":829132, "CGAGG":920621, "CGTAA":1038328, "CGTAT":1275300, "CGTAC":1009423, "CGTAG":979892, "CGTTA":1398557, "CGTTT":1912953, "CGTTC":1436056, "CGTTG":1349307, "CGTCA":1662016, "CGTCT":2038544, "CGTCC":1322207, "CGTCG":6993634, "CGTGA":975875, "CGTGT":7221323, "CGTGC":977734, "CGTGG":1226805, "CGCAA":1220584, "CGCAT":985340, "CGCAC":1722909, "CGCAG":1302885, "CGCTA":1324362, "CGCTT":1142456, "CGCTC":2798628, "CGCTG":1749073, "CGCCA":1596475, "CGCCT":1235935, "CGCCC":1641326, "CGCCG":1063261, "CGCGA":321710, "CGCGT":351901, "CGCGC":722663, "CGCGG":548729, "CGGAA":9591863, "CGGAT":749047, "CGGAC":964708, "CGGAG":1135967, "CGGTA":940444, "CGGTT":892149, "CGGTC":1020007, "CGGTG":1991014, "CGGCA":1131409, "CGGCT":1060550, "CGGCC":1188322, "CGGCG":620524, "CGGGA":824070, 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"GAGCA":2604841, "GAGCT":2512459, "GAGCC":2153539, "GAGCG":8255378, "GAGGA":3432501, "GAGGT":2173744, "GAGGC":1932019, "GAGGG":2544168, "GTAAA":4249703, "GTAAT":3567870, "GTAAC":6006129, "GTAAG":1716339, "GTATA":3903618, "GTATT":4538882, "GTATC":3037924, "GTATG":2891448, "GTACA":4679526, "GTACT":3633616, "GTACC":2884664, "GTACG":790103, "GTAGA":4325556, "GTAGT":2529246, "GTAGC":2173661, "GTAGG":6701765, "GTTAA":3427068, "GTTAT":3794078, "GTTAC":3389406, "GTTAG":2377301, "GTTTA":4480051, "GTTTT":7124890, "GTTTC":4456474, "GTTTG":4828458, "GTTCA":4448632, "GTTCT":4937026, "GTTCC":3502692, "GTTCG":739497, "GTTGA":3551777, "GTTGT":4628766, "GTTGC":2850820, "GTTGG":2448707, "GTCAA":3797340, "GTCAT":4604455, "GTCAC":3858911, "GTCAG":3663174, "GTCTA":3552030, "GTCTT":4553158, "GTCTC":8331649, "GTCTG":6371974, "GTCCA":3861021, "GTCCT":4388097, "GTCCC":3211350, "GTCCG":833977, "GTCGA":796883, "GTCGT":7286838, "GTCGC":1374983, "GTCGG":891074, "GTGAA":3357124, "GTGAT":2849655, "GTGAC":2697994, "GTGAG":2400915, "GTGTA":13943700, "GTGTT":5838444, "GTGTC":4181620, "GTGTG":12189702, "GTGCA":2906911, "GTGCT":2827273, "GTGCC":2131012, "GTGCG":904155, "GTGGA":3109011, "GTGGT":3694285, "GTGGC":2060716, "GTGGG":2985187, "GCAAA":3296651, "GCAAT":2598536, "GCAAC":3394637, "GCAAG":2248279, "GCATA":2390635, "GCATT":3593055, "GCATC":2692556, "GCATG":4737030, "GCACA":4837661, "GCACT":3018062, "GCACC":2764820, "GCACG":996025, "GCAGA":3957086, "GCAGT":2886975, "GCAGC":3753921, "GCAGG":2540742, "GCTAA":2605059, "GCTAT":2920042, "GCTAC":3962372, "GCTAG":2173978, "GCTTA":1667018, "GCTTT":4128136, "GCTTC":3431050, "GCTTG":1942404, "GCTCA":3203732, "GCTCT":5905751, "GCTCC":5879892, "GCTCG":998229, "GCTGA":3256055, "GCTGT":4843022, "GCTGC":6022530, "GCTGG":3125829, "GCCAA":3292447, "GCCAT":3651231, "GCCAC":3637431, "GCCAG":3102297, "GCCTA":2484358, "GCCTT":3198754, "GCCTC":3464836, "GCCTG":2788196, "GCCCA":3244676, "GCCCT":2983456, "GCCCC":3024265, "GCCCG":841250, "GCCGA":876686, "GCCGT":1240910, 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"GGCCT":2521321, "GGCCC":1953302, "GGCCG":937810, "GGCGA":707519, "GGCGT":662220, "GGCGC":827818, "GGCGG":1216241, "GGGAA":6909781, "GGGAT":1703151, "GGGAC":1742720, "GGGAG":2486192, "GGGTA":1587263, "GGGTT":1874282, "GGGTC":1357331, "GGGTG":4765857, "GGGCA":1681840, "GGGCT":1502376, "GGGCC":1537945, "GGGCG":1029777, "GGGGA":1914505, "GGGGT":2575100, "GGGGC":1559297, "GGGGG":29968743 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 45206271, "total_bases": 3604645997, "q20_bases": 3500463477, "q30_bases": 3324965151, "total_cycles": 86, "quality_curves": { 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"T":[35.9161,35.9185,35.8895,35.8957,35.8916,35.8775,35.8873,35.8564,35.8189,35.8103,35.728,35.6947,35.7132,35.7039,35.6714,35.6549,35.6746,35.6367,35.6328,35.6427,35.5913,35.6034,35.5939,35.5547,35.5879,35.5651,35.5614,35.5648,35.5218,35.5137,35.5212,35.5074,35.4705,35.4937,35.4485,35.4174,35.4259,35.3788,35.3921,35.3683,35.4399,35.3368,35.3963,35.3574,35.3158,35.4104,35.3172,35.2672,35.3542,35.2767,35.1956,35.1999,35.232,35.1877,35.2014,35.2389,35.2032,35.2018,35.1838,35.1347,35.0961,35.1108,35.0621,35.1077,35.0913,35.0487,35.0194,35.0796,35.0497,35.0675,34.9959,34.9803,34.9843,35.0141,34.9202,34.9834,34.9679,34.9528,34.9162,34.9289,34.8376,34.8821,34.8428,34.8111,34.8258,34.7893], "C":[36.3606,36.3572,36.3411,36.3383,36.3296,36.3257,36.3035,36.2979,36.2672,36.2638,36.2085,36.2168,36.22,36.2065,36.1777,36.1733,36.165,36.1645,36.156,36.1452,36.1303,36.1437,36.1161,36.1047,36.0859,36.085,36.0569,36.0466,36.0212,36.0321,36.0492,36.0192,36.0052,36.0121,35.9949,36.0001,35.9524,35.967,35.9234,35.9287,35.8821,35.9014,35.9036,35.8937,35.8703,35.869,35.8325,35.8324,35.7802,35.8025,35.7596,35.7823,35.7815,35.7848,35.7884,35.7788,35.7528,35.7394,35.713,35.6901,35.6718,35.6568,35.6544,35.6495,35.657,35.602,35.607,35.6058,35.5781,35.5673,35.5394,35.5275,35.5269,35.5284,35.4901,35.5007,35.5069,35.4867,35.4752,35.4486,35.4209,35.4007,35.4127,35.3617,35.3862,35.2517], "G":[35.9752,35.9752,35.9798,35.9751,35.9727,35.9693,35.9381,35.9376,35.9216,35.9164,35.8189,35.8243,35.811,35.8074,35.8065,35.7831,35.7745,35.7781,35.7572,35.7464,35.7278,35.7042,35.6803,35.6979,35.6664,35.6581,35.633,35.6071,35.5771,35.5921,35.5937,35.4816,35.5376,35.5553,35.4885,35.5447,35.4955,35.5048,35.4688,35.4317,35.4378,35.4197,35.4191,35.3825,35.3179,35.3685,35.3382,35.365,35.3034,35.3041,35.2374,35.3187,35.2566,35.2496,35.2329,35.2279,35.3079,35.2628,35.2298,35.219,35.1934,35.1542,35.1913,35.1521,35.1107,35.0917,35.058,35.043,35.0609,34.9866,34.9507,34.9331,34.959,34.917,34.9377,34.8756,34.9108,34.9416,34.9029,34.8205,34.8479,34.8093,34.7878,34.747,34.7615,34.5898], 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"CTTCT":2814333, "CTTCC":1779368, "CTTCG":543516, "CTTGA":2977387, "CTTGT":3382328, "CTTGC":1655234, "CTTGG":2509959, "CTCAA":2848374, "CTCAT":2370311, "CTCAC":1609380, "CTCAG":2783815, "CTCTA":2022280, "CTCTT":2403253, "CTCTC":4726992, "CTCTG":4326435, "CTCCA":2692350, "CTCCT":2674244, "CTCCC":1503614, "CTCCG":805652, "CTCGA":715879, "CTCGT":902296, "CTCGC":518842, "CTCGG":780560, "CTGAA":3990642, "CTGAT":3244345, "CTGAC":2838489, "CTGAG":3803063, "CTGTA":5374020, "CTGTT":5150123, "CTGTC":4489642, "CTGTG":9110127, "CTGCA":3497535, "CTGCT":3289532, "CTGCC":1930466, "CTGCG":816397, "CTGGA":3927458, "CTGGT":4019802, "CTGGC":2100179, "CTGGG":3661713, "CCAAA":3348920, "CCAAT":2277606, "CCAAC":1728916, "CCAAG":1640388, "CCATA":2112816, "CCATT":2939429, "CCATC":1844581, "CCATG":2728457, "CCACA":2860048, "CCACT":2011562, "CCACC":1518700, "CCACG":717154, "CCAGA":2921281, "CCAGT":3177932, "CCAGC":2364977, "CCAGG":2903551, "CCTAA":1587652, "CCTAT":1467385, "CCTAC":1306262, "CCTAG":1554648, "CCTTA":1266437, "CCTTT":2446366, "CCTTC":1795753, "CCTTG":2293722, "CCTCA":2017510, "CCTCT":2633942, "CCTCC":1919458, "CCTCG":691373, "CCTGA":2273582, "CCTGT":3599649, "CCTGC":1873122, "CCTGG":2624090, "CCCAA":1518971, "CCCAT":1635868, "CCCAC":1280826, "CCCAG":1925019, "CCCTA":937017, "CCCTT":1314258, "CCCTC":1462737, "CCCTG":1939403, "CCCCA":1279223, "CCCCT":1116414, "CCCCC":834854, "CCCCG":515906, "CCCGA":515084, "CCCGT":502918, "CCCGC":358627, "CCCGG":492863, "CCGAA":773013, "CCGAT":838164, "CCGAC":564949, "CCGAG":725059, "CCGTA":649002, "CCGTT":918796, "CCGTC":741750, "CCGTG":995316, "CCGCA":472910, "CCGCT":560676, "CCGCC":500601, "CCGCG":213119, "CCGGA":546847, "CCGGT":797553, "CCGGC":452683, "CCGGG":736426, "CGAAA":954584, "CGAAT":751954, "CGAAC":566318, "CGAAG":886848, "CGATA":1025260, "CGATT":1000976, "CGATC":783654, "CGATG":1404578, "CGACA":1075600, "CGACT":656001, "CGACC":471151, "CGACG":312390, "CGAGA":1875884, "CGAGT":830947, "CGAGC":758407, "CGAGG":1169423, "CGTAA":889593, "CGTAT":788223, "CGTAC":635803, "CGTAG":1118631, "CGTTA":925957, "CGTTT":1432719, "CGTTC":894343, "CGTTG":1393011, "CGTCA":1083409, "CGTCT":1077282, "CGTCC":655107, "CGTCG":451015, "CGTGA":1065250, "CGTGT":1962780, "CGTGC":752426, "CGTGG":1238509, "CGCAA":657507, "CGCAT":602433, "CGCAC":714502, "CGCAG":807585, "CGCTA":540110, "CGCTT":520542, "CGCTC":643838, "CGCTG":1035129, "CGCCA":617237, "CGCCT":643940, "CGCCC":352656, "CGCCG":368463, "CGCGA":252445, "CGCGT":288504, "CGCGC":286298, "CGCGG":256001, "CGGAA":1022619, "CGGAT":740218, "CGGAC":648960, "CGGAG":1265900, "CGGTA":999922, "CGGTT":974212, "CGGTC":719693, "CGGTG":1391251, "CGGCA":799803, "CGGCT":676555, "CGGCC":595717, "CGGCG":359582, "CGGGA":944239, "CGGGT":718958, "CGGGC":542921, "CGGGG":1191032, "GAAAA":6543544, "GAAAT":4800117, "GAAAC":3597085, "GAAAG":7350683, "GAATA":4700606, "GAATT":2782212, "GAATC":2011123, "GAATG":4901685, "GAACA":5608891, "GAACT":2447914, "GAACC":1484079, "GAACG":1127865, "GAAGA":7114495, "GAAGT":4533190, "GAAGC":2801096, "GAAGG":5349757, "GATAA":3575050, "GATAT":4997961, "GATAC":2319918, "GATAG":5508631, "GATTA":2691188, "GATTT":3548242, "GATTC":1741702, "GATTG":3172364, "GATCA":2587794, "GATCT":2353341, "GATCC":1295890, "GATCG":920233, "GATGA":5803064, "GATGT":6087613, "GATGC":2071667, "GATGG":5935348, "GACAA":4448858, "GACAT":3699454, "GACAC":3216877, "GACAG":10120158, "GACTA":2645049, "GACTT":2045235, "GACTC":1493944, "GACTG":4316017, "GACCA":3006328, "GACCT":1892139, "GACCC":994458, "GACCG":760846, "GACGA":1231349, "GACGT":1099988, "GACGC":585516, "GACGG":1282633, "GAGAA":10053320, "GAGAT":5851423, "GAGAC":6690745, "GAGAG":37736015, "GAGTA":4502366, "GAGTT":3232791, "GAGTC":2482637, "GAGTG":6912885, "GAGCA":6030668, "GAGCT":2944045, "GAGCC":1702938, "GAGCG":2160040, "GAGGA":10246138, "GAGGT":5910385, "GAGGC":2703343, "GAGGG":8388690, "GTAAA":5023535, "GTAAT":4222570, "GTAAC":2911454, "GTAAG":3146287, "GTATA":4706735, "GTATT":4788467, "GTATC":1845118, "GTATG":5396013, "GTACA":4375493, "GTACT":3125070, "GTACC":1675854, "GTACG":806564, "GTAGA":7424953, "GTAGT":10414150, "GTAGC":3131687, "GTAGG":6663838, "GTTAA":4990959, "GTTAT":4508758, "GTTAC":2400166, "GTTAG":5042648, "GTTTA":4538131, "GTTTT":5497621, "GTTTC":3037060, "GTTTG":5385234, "GTTCA":3169140, "GTTCT":3113388, "GTTCC":1879897, "GTTCG":660144, "GTTGA":5043441, "GTTGT":15890765, "GTTGC":2690542, "GTTGG":5871380, "GTCAA":3676995, "GTCAT":3495853, "GTCAC":2087278, "GTCAG":4900289, "GTCTA":2638166, "GTCTT":2922958, "GTCTC":3074598, "GTCTG":5823849, "GTCCA":3018595, "GTCCT":2392926, "GTCCC":1296119, "GTCCG":724297, "GTCGA":929171, "GTCGT":1402674, "GTCGC":510831, "GTCGG":968902, "GTGAA":4819230, "GTGAT":5078853, "GTGAC":2964228, "GTGAG":6952737, "GTGTA":7400916, "GTGTT":7983281, "GTGTC":4962473, "GTGTG":36507471, "GTGCA":3421793, "GTGCT":2840251, "GTGCC":1580454, "GTGCG":1273056, "GTGGA":5803095, "GTGGT":11471629, "GTGGC":2886992, "GTGGG":6075257, "GCAAA":3460532, "GCAAT":2328302, "GCAAC":2219890, "GCAAG":2078617, "GCATA":2235055, "GCATT":3137348, "GCATC":1872413, "GCATG":2996241, "GCACA":2932304, "GCACT":2119767, "GCACC":1249793, "GCACG":828509, "GCAGA":4060564, "GCAGT":4364512, "GCAGC":4912427, "GCAGG":3554344, "GCTAA":2266077, "GCTAT":2292217, "GCTAC":1823879, "GCTAG":2275525, "GCTTA":1312444, "GCTTT":2582557, "GCTTC":1701181, "GCTTG":2275386, "GCTCA":2141952, "GCTCT":2134090, "GCTCC":1425092, "GCTCG":593518, "GCTGA":3195635, "GCTGT":6971455, "GCTGC":2995147, "GCTGG":3483601, "GCCAA":2033374, "GCCAT":2317705, "GCCAC":1565266, "GCCAG":2423072, "GCCTA":1686737, "GCCTT":1841013, "GCCTC":1461303, "GCCTG":2362737, "GCCCA":1387130, "GCCCT":1212038, "GCCCC":799572, "GCCCG":435705, "GCCGA":689587, "GCCGT":868459, "GCCGC":472028, "GCCGG":743971, "GCGAA":675422, "GCGAT":1011905, "GCGAC":576061, "GCGAG":1856410, "GCGTA":697087, "GCGTT":1019468, "GCGTC":649682, "GCGTG":1695592, "GCGCA":715186, "GCGCT":678039, "GCGCC":477594, "GCGCG":395255, "GCGGA":786449, "GCGGT":1097879, "GCGGC":707681, "GCGGG":869438, "GGAAA":5514218, "GGAAT":3429499, "GGAAC":2784003, "GGAAG":5403174, "GGATA":3935053, "GGATT":2477508, "GGATC":1670794, "GGATG":4861979, "GGACA":4753239, "GGACT":2583617, "GGACC":1481008, "GGACG":1042642, "GGAGA":10673342, "GGAGT":4458046, "GGAGC":4661536, "GGAGG":8535679, "GGTAA":3554634, "GGTAT":3424742, "GGTAC":2431978, "GGTAG":7839387, "GGTTA":5628315, "GGTTT":4320427, "GGTTC":1978772, "GGTTG":7688855, "GGTCA":3335008, "GGTCT":3037311, "GGTCC":1592670, "GGTCG":1021011, "GGTGA":4765782, "GGTGT":6582793, "GGTGC":2218054, "GGTGG":6226331, "GGCAA":2417674, "GGCAT":2237519, "GGCAC":1694206, "GGCAG":4134351, "GGCTA":2574998, "GGCTT":1832999, "GGCTC":1555902, "GGCTG":3762799, "GGCCA":2211683, "GGCCT":2082103, "GGCCC":1115592, "GGCCG":802386, "GGCGA":903237, "GGCGT":947116, "GGCGC":533852, "GGCGG":1105816, "GGGAA":4620087, "GGGAT":3493940, "GGGAC":2583522, "GGGAG":8572300, "GGGTA":4548442, "GGGTT":5622861, "GGGTC":2431650, "GGGTG":4770430, "GGGCA":2476357, "GGGCT":2537928, "GGGCC":1476427, "GGGCG":1039318, "GGGGA":5182188, "GGGGT":5924293, "GGGGC":2214332, "GGGGG":5001026 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 45206271, "total_bases": 3719132852, "q20_bases": 3600860405, "q30_bases": 3407692255, "total_cycles": 86, "quality_curves": { 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"T":[35.9591,35.9267,35.9437,35.933,35.889,35.8908,35.8382,35.8347,35.8109,35.8111,35.8123,35.7856,35.7779,35.7616,35.7464,35.7163,35.7262,35.6862,35.6906,35.6635,35.6541,35.6478,35.6481,35.6047,35.5936,35.5472,35.5729,35.5426,35.5294,35.4918,35.4921,35.4421,35.4356,35.4211,35.3879,35.3646,35.3637,35.3258,35.3351,35.2918,35.275,35.2495,35.2331,35.2118,35.1821,35.1439,35.1479,35.149,35.0995,35.0706,35.0551,35.0337,35.0334,34.9717,34.9593,34.938,34.938,34.9169,34.898,34.8632,34.8382,34.8099,34.7977,34.8165,34.8103,34.8891,34.8251,34.8316,34.7736,34.7735,34.7332,34.6958,34.6749,34.6204,34.5807,34.6297,34.6,34.6198,34.592,34.5469,34.4569,34.4609,34.4528,34.4667,34.4316,34.0332], "C":[36.4064,36.411,36.4073,36.4051,36.3618,36.3708,36.3488,36.3392,36.3127,36.3176,36.3181,36.2838,36.2556,36.2703,36.232,36.2382,36.2301,36.2072,36.2039,36.1742,36.1767,36.1593,36.1482,36.146,36.13,36.1173,36.1074,36.0877,36.0583,36.0336,36.0179,36.0248,35.9946,35.9789,35.9607,35.9548,35.9366,35.9304,35.8953,35.8629,35.8542,35.8343,35.8341,35.8168,35.7768,35.7578,35.7381,35.7267,35.7252,35.6576,35.6398,35.6319,35.6493,35.5689,35.5717,35.5268,35.5064,35.5067,35.5359,35.5039,35.4877,35.4402,35.4321,35.4238,35.3829,35.3707,35.2918,35.2791,35.2362,35.2688,35.181,35.1957,35.1607,35.1211,35.093,35.1183,35.0971,35.0331,35.0688,35.0029,34.9638,34.9698,34.9906,34.9138,34.919,34.6266], "G":[36.1944,36.177,36.1976,36.1899,36.1267,36.1273,36.1039,36.0971,36.0664,36.0655,36.0774,36.0337,36.0107,36.0206,35.9944,35.9749,35.9629,35.9482,35.9644,35.934,35.9185,35.8963,35.8896,35.8723,35.8566,35.8474,35.8288,35.8288,35.8203,35.7931,35.7786,35.7709,35.7128,35.6921,35.6955,35.6697,35.646,35.5914,35.6118,35.5527,35.5835,35.5634,35.55,35.5208,35.5107,35.4515,35.47,35.4183,35.4452,35.3647,35.3759,35.3374,35.3452,35.2524,35.2636,35.209,35.2474,35.1693,35.195,35.1387,35.1108,35.063,35.0594,35.0063,35.0166,34.9506,35.0166,34.9352,34.9492,34.9354,34.9035,34.8384,34.845,34.7802,34.7572,34.6804,34.8034,34.6486,34.7375,34.6045,34.643,34.5723,34.6349,34.5506,34.5314,34.1875], 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"A":[0.28034,0.27452,0.286254,0.282179,0.286823,0.277681,0.277005,0.274906,0.283312,0.276151,0.282124,0.279266,0.277033,0.273664,0.283371,0.276564,0.28167,0.277957,0.282649,0.27738,0.284206,0.276005,0.284709,0.274568,0.283779,0.279495,0.285865,0.27375,0.283261,0.274634,0.281685,0.275013,0.285938,0.270555,0.281949,0.277743,0.285398,0.276447,0.283888,0.276763,0.284687,0.278624,0.287619,0.279786,0.284411,0.277381,0.287135,0.277321,0.280058,0.27746,0.284833,0.277626,0.285812,0.280693,0.279933,0.274753,0.282407,0.276362,0.282574,0.279049,0.284326,0.278304,0.288135,0.281517,0.290656,0.280833,0.285161,0.283063,0.288038,0.278199,0.286296,0.277167,0.281058,0.275853,0.283362,0.270013,0.278571,0.276195,0.281372,0.272185,0.276591,0.272266,0.278643,0.273248,0.282503,0.273976], 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"CATGC":2977854, "CATGG":4682879, "CACAA":8749696, "CACAT":7728758, "CACAC":36091330, "CACAG":9308177, "CACTA":7531419, "CACTT":4361366, "CACTC":6872356, "CACTG":6474190, "CACCA":7839944, "CACCT":5491704, "CACCC":4636018, "CACCG":1449668, "CACGA":1019516, "CACGT":1405913, "CACGC":1793751, "CACGG":992406, "CAGAA":4179943, "CAGAT":4654693, "CAGAC":5718284, "CAGAG":4560949, "CAGTA":4569104, "CAGTT":4189444, "CAGTC":4325127, "CAGTG":3763327, "CAGCA":5002382, "CAGCT":6984885, "CAGCC":3691498, "CAGCG":1054645, "CAGGA":3331404, "CAGGT":2967775, "CAGGC":2426266, "CAGGG":2064996, "CTAAA":4135093, "CTAAT":3232508, "CTAAC":4966019, "CTAAG":1345882, "CTATA":5662167, "CTATT":4631412, "CTATC":5378636, "CTATG":2916512, "CTACA":7995499, "CTACT":10544122, "CTACC":7573914, "CTACG":1160161, "CTAGA":2584032, "CTAGT":2773430, "CTAGC":2309662, "CTAGG":1593960, "CTTAA":2816103, "CTTAT":3493968, "CTTAC":3001222, "CTTAG":1614369, "CTTTA":4552261, "CTTTT":6000592, "CTTTC":6846757, "CTTTG":3980969, "CTTCA":5683450, "CTTCT":7635349, "CTTCC":5097936, "CTTCG":900222, "CTTGA":2563260, "CTTGT":3665068, "CTTGC":1998874, "CTTGG":1611034, "CTCAA":4631821, "CTCAT":5126502, "CTCAC":6830834, "CTCAG":3906597, "CTCTA":7937106, "CTCTT":8139507, "CTCTC":35909085, "CTCTG":9260571, "CTCCA":7793335, "CTCCT":10059202, "CTCCC":8029962, "CTCCG":1274270, "CTCGA":753080, "CTCGT":1197945, "CTCGC":1833106, "CTCGG":1559725, "CTGAA":4036974, "CTGAT":3702031, "CTGAC":4876318, "CTGAG":2871635, "CTGTA":6144866, "CTGTT":6741682, "CTGTC":9966084, "CTGTG":4880982, "CTGCA":6630528, "CTGCT":7344213, "CTGCC":4116826, "CTGCG":820148, "CTGGA":3716798, "CTGGT":3697757, "CTGGC":2423221, "CTGGG":1996981, "CCAAA":4557623, "CCAAT":3483267, "CCAAC":5989165, "CCAAG":2663420, "CCATA":4349248, "CCATT":4689008, "CCATC":5777295, "CCATG":3602506, "CCACA":11852717, "CCACT":6906842, "CCACC":5992266, "CCACG":1418653, "CCAGA":4385252, "CCAGT":3791627, "CCAGC":3477042, "CCAGG":2718453, "CCTAA":3315309, "CCTAT":3177492, "CCTAC":6415407, "CCTAG":2153785, "CCTTA":3205968, "CCTTT":4977545, "CCTTC":5304528, "CCTTG":2559053, "CCTCA":5784107, "CCTCT":10917246, "CCTCC":7792986, "CCTCG":1118338, "CCTGA":3474334, "CCTGT":5301405, "CCTGC":3516197, "CCTGG":2880745, "CCCAA":4445843, "CCCAT":3858589, "CCCAC":5940670, "CCCAG":3855840, "CCCTA":5184408, "CCCTT":4036838, "CCCTC":7542973, "CCCTG":4294458, "CCCCA":5373926, "CCCCT":5908078, "CCCCC":4284244, "CCCCG":1231723, "CCCGA":762258, "CCCGT":1032508, "CCCGC":925377, "CCCGG":716190, "CCGAA":656963, "CCGAT":892417, "CCGAC":952718, "CCGAG":832087, "CCGTA":1082202, "CCGTT":1081027, "CCGTC":1269588, "CCGTG":933310, "CCGCA":930256, "CCGCT":1194162, "CCGCC":1134999, "CCGCG":279492, "CCGGA":643544, "CCGGT":727208, "CCGGC":705820, "CCGGG":589131, "CGAAA":846752, "CGAAT":653430, "CGAAC":687048, "CGAAG":567975, "CGATA":804730, "CGATT":866819, "CGATC":762381, "CGATG":866013, "CGACA":1533224, "CGACT":873606, "CGACC":1095968, "CGACG":453761, "CGAGA":1089226, "CGAGT":588316, "CGAGC":619547, "CGAGG":699847, "CGTAA":820343, "CGTAT":960155, "CGTAC":807487, "CGTAG":781060, "CGTTA":1121941, "CGTTT":1559811, "CGTTC":1148154, "CGTTG":1069185, "CGTCA":1282104, "CGTCT":1602237, "CGTCC":1035454, "CGTCG":2556339, "CGTGA":750503, "CGTGT":3359419, "CGTGC":768258, "CGTGG":835448, "CGCAA":956183, "CGCAT":778187, "CGCAC":1320020, "CGCAG":921429, "CGCTA":1020106, "CGCTT":898344, "CGCTC":2148856, "CGCTG":1373732, "CGCCA":1175478, "CGCCT":948264, "CGCCC":1118026, "CGCCG":683778, "CGCGA":227738, "CGCGT":253482, "CGCGC":425947, "CGCGG":247529, "CGGAA":3161109, "CGGAT":572557, "CGGAC":718203, "CGGAG":836734, "CGGTA":742492, "CGGTT":701974, "CGGTC":767023, "CGGTG":1534724, "CGGCA":835520, "CGGCT":812362, "CGGCC":818511, "CGGCG":359428, "CGGGA":599990, "CGGGT":459726, "CGGGC":453611, "CGGGG":529981, "GAAAA":3943233, "GAAAT":3712375, "GAAAC":3185077, "GAAAG":4691987, "GAATA":2705994, "GAATT":2489496, "GAATC":1795039, "GAATG":2632433, "GAACA":3998026, "GAACT":2284365, "GAACC":2046048, "GAACG":957492, "GAAGA":5249594, "GAAGT":2219652, "GAAGC":1755150, "GAAGG":1891822, "GATAA":2108520, "GATAT":2628030, "GATAC":1951379, "GATAG":1398573, "GATTA":2245607, "GATTT":3382559, "GATTC":1932186, "GATTG":2070836, "GATCA":2406237, "GATCT":3437970, "GATCC":1679134, "GATCG":3171380, "GATGA":2862500, "GATGT":3041736, "GATGC":1873963, "GATGG":1960342, "GACAA":4266273, "GACAT":4006581, "GACAC":5190156, "GACAG":4604213, "GACTA":2375313, "GACTT":2382828, "GACTC":2515282, "GACTG":3044845, "GACCA":3647125, "GACCT":2393226, "GACCC":2333231, "GACCG":714946, "GACGA":1054811, "GACGT":891270, "GACGC":708845, "GACGG":756429, "GAGAA":3237649, "GAGAT":2934982, "GAGAC":3241615, "GAGAG":5058425, "GAGTA":1659286, "GAGTT":1889407, "GAGTC":1573418, "GAGTG":4069063, "GAGCA":2087231, "GAGCT":2035178, "GAGCC":1730543, "GAGCG":2991002, "GAGGA":2743963, "GAGGT":1720349, "GAGGC":1554395, "GAGGG":1664986, "GTAAA":3508368, "GTAAT":2994451, "GTAAC":4587446, "GTAAG":1364543, "GTATA":3243074, "GTATT":3826256, "GTATC":2424110, "GTATG":2349156, "GTACA":3818451, "GTACT":2998620, "GTACC":2347956, "GTACG":644000, "GTAGA":3487177, "GTAGT":2050549, "GTAGC":1778770, "GTAGG":3436615, "GTTAA":2791840, "GTTAT":3139510, "GTTAC":2761694, "GTTAG":1880629, "GTTTA":3600427, "GTTTT":5688337, "GTTTC":3568324, "GTTTG":3750553, "GTTCA":3534033, "GTTCT":3963683, "GTTCC":2803892, "GTTCG":572285, "GTTGA":2817734, "GTTGT":3618328, "GTTGC":2280821, "GTTGG":1747290, "GTCAA":3014836, "GTCAT":3733669, "GTCAC":3052678, "GTCAG":2855060, "GTCTA":2817857, "GTCTT":3649840, "GTCTC":6619943, "GTCTG":5058263, "GTCCA":3032946, "GTCCT":3493610, "GTCCC":2539369, "GTCCG":645333, "GTCGA":606418, "GTCGT":3014420, "GTCGC":1001620, "GTCGG":573589, "GTGAA":2679521, "GTGAT":2284566, "GTGAC":2163488, "GTGAG":1780352, "GTGTA":8612085, "GTGTT":4595147, "GTGTC":3298118, "GTGTG":9218489, "GTGCA":2337070, "GTGCT":2288783, "GTGCC":1695118, "GTGCG":674255, "GTGGA":2382011, "GTGGT":2677488, "GTGGC":1611147, "GTGGG":1328617, "GCAAA":2644761, "GCAAT":2111217, "GCAAC":2705350, "GCAAG":1744260, "GCATA":1945678, "GCATT":2940720, "GCATC":2164229, "GCATG":4054979, "GCACA":3809941, "GCACT":2384111, "GCACC":2200894, "GCACG":772494, "GCAGA":2727212, "GCAGT":2288393, "GCAGC":3006992, "GCAGG":1981725, "GCTAA":2075042, "GCTAT":2370899, "GCTAC":3173398, "GCTAG":1662511, "GCTTA":1345604, "GCTTT":3352924, "GCTTC":2743823, "GCTTG":1557586, "GCTCA":2525637, "GCTCT":4690121, "GCTCC":4710803, "GCTCG":741160, "GCTGA":2587815, "GCTGT":3879227, "GCTGC":4900690, "GCTGG":2447179, "GCCAA":2559943, "GCCAT":2936647, "GCCAC":2852304, "GCCAG":2206008, "GCCTA":1990492, "GCCTT":2575271, "GCCTC":2718365, "GCCTG":2194670, "GCCCA":2469115, "GCCCT":2367749, "GCCCC":2182244, "GCCCG":572877, "GCCGA":653495, "GCCGT":903204, "GCCGC":700611, "GCCGG":581688, "GCGAA":540950, "GCGAT":508983, "GCGAC":541930, "GCGAG":528757, "GCGTA":454563, "GCGTT":763010, "GCGTC":2854640, "GCGTG":714301, "GCGCA":651345, "GCGCT":807933, "GCGCC":584694, "GCGCG":297388, "GCGGA":435917, "GCGGT":534867, "GCGGC":539540, "GCGGG":386300, "GGAAA":5179694, "GGAAT":2023322, "GGAAC":2017383, "GGAAG":4234023, "GGATA":1530030, "GGATT":1891843, "GGATC":1436414, "GGATG":1890459, "GGACA":3641109, "GGACT":1926695, "GGACC":1600996, "GGACG":694879, "GGAGA":2957979, "GGAGT":1666069, "GGAGC":1548288, "GGAGG":2120875, "GGTAA":1699167, "GGTAT":1748531, "GGTAC":1748352, "GGTAG":1348493, "GGTTA":1674841, "GGTTT":2469384, "GGTTC":1497129, "GGTTG":1585653, "GGTCA":2337172, "GGTCT":2431240, "GGTCC":1458882, "GGTCG":912619, "GGTGA":1827474, "GGTGT":3995902, "GGTGC":1282712, "GGTGG":2163894, "GGCAA":1715916, "GGCAT":1710218, "GGCAC":1604502, "GGCAG":2009715, "GGCTA":1898108, "GGCTT":1710384, "GGCTC":1715797, "GGCTG":2129238, "GGCCA":2230316, "GGCCT":2003999, "GGCCC":1451013, "GGCCG":624976, "GGCGA":537900, "GGCGT":479470, "GGCGC":522957, "GGCGG":534089, "GGGAA":3895700, "GGGAT":1319885, "GGGAC":1330642, "GGGAG":1610510, "GGGTA":1180510, "GGGTT":1298307, "GGGTC":1027831, "GGGTG":2924814, "GGGCA":1281115, "GGGCT":1150451, "GGGCC":1111880, "GGGCG":390563, "GGGGA":1280895, "GGGGT":1038515, "GGGGC":807675, "GGGGG":1157719 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213516_sample_1703676_S128_L002_R1_001.fastq.gz --in2 lib_213516_sample_1703676_S128_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213516_sample_1703676_S128_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213516_sample_1703676_S128_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213516_sample_1703676_S128_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213516_sample_1703676_S128_L002_R2_001.fastp-trim.fq.gz " }