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"GC":[0.668674,0.50816,0.417846,0.430337,0.407055,0.401475,0.342445,0.421659,0.458863,0.349439,0.502404,0.477648,0.428063,0.41548,0.41601,0.414203,0.439368,0.418538,0.431827,0.426096,0.43074,0.422656,0.437329,0.418626,0.428201,0.430849,0.426638,0.419029,0.441008,0.416357,0.426303,0.425504,0.428139,0.420255,0.435406,0.421203,0.428175,0.428592,0.430013,0.417892,0.439249,0.419082,0.432345,0.430392,0.427362,0.424155,0.439175,0.420086,0.432097,0.429885,0.428236,0.417328,0.43937,0.423046,0.430085,0.430157,0.428407,0.42179,0.439625,0.420991,0.431809,0.43108,0.432672,0.427182,0.443151,0.421721,0.436052,0.434037,0.432265,0.424182,0.443838,0.425348,0.433244,0.437539,0.435218,0.429157,0.443429,0.425955,0.434742,0.437105,0.435143,0.428521,0.443889,0.428675,0.439465,0.439923,0.43827,0.432517,0.44986,0.430549,0.442006,0.441767,0.439837,0.435734,0.44833,0.435374,0.442367,0.442497,0.441806,0.433529,0.453926] }, "kmer_count": { "AAAAA":14872135, "AAAAT":9585621, "AAAAC":6657617, "AAAAG":7780896, 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"TACCA":2585771, "TACCT":2461723, "TACCC":1225506, "TACCG":768259, "TACGA":939541, "TACGT":1158110, "TACGC":496498, "TACGG":1031138, "TAGAA":5886972, "TAGAT":7681066, "TAGAC":3192705, "TAGAG":9551066, "TAGTA":10256911, "TAGTT":6481524, "TAGTC":2764919, "TAGTG":8827623, "TAGCA":3643480, "TAGCT":3427472, "TAGCC":2145325, "TAGCG":1133703, "TAGGA":5147881, "TAGGT":4557948, "TAGGC":2305491, "TAGGG":6603658, "TTAAA":7233083, "TTAAT":6401133, "TTAAC":3173576, "TTAAG":3315011, "TTATA":5439609, "TTATT":6823807, "TTATC":2347625, "TTATG":4994220, "TTACA":5356000, "TTACT":3409297, "TTACC":1803138, "TTACG":926672, "TTAGA":4954501, "TTAGT":5043202, "TTAGC":2260463, "TTAGG":3935019, "TTTAA":6507331, "TTTAT":6339052, "TTTAC":3868829, "TTTAG":5045008, "TTTTA":6768791, "TTTTT":11631722, "TTTTC":4437443, "TTTTG":7133750, "TTTCA":5293080, "TTTCT":5397335, "TTTCC":3345180, "TTTCG":1006861, "TTTGA":6148846, "TTTGT":7980612, "TTTGC":3109604, "TTTGG":5430626, "TTCAA":4719665, "TTCAT":4478350, "TTCAC":2977736, 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"CATAG":3403718, "CATTA":4386504, "CATTT":6382006, "CATTC":2913378, "CATTG":4559163, "CATCA":4054089, "CATCT":3529208, "CATCC":2017383, "CATCG":957657, "CATGA":3991220, "CATGT":5615859, "CATGC":2322145, "CATGG":5308414, "CACAA":4731318, "CACAT":5426507, "CACAC":14449029, "CACAG":5530309, "CACTA":2660032, "CACTT":2815561, "CACTC":2055438, "CACTG":4122814, "CACCA":3122810, "CACCT":2243647, "CACCC":1361699, "CACCG":925842, "CACGA":970697, "CACGT":4854317, "CACGC":805820, "CACGG":1056229, "CAGAA":6025455, "CAGAT":6229956, "CAGAC":5719489, "CAGAG":11190359, "CAGTA":7817903, "CAGTT":4718677, "CAGTC":5149909, "CAGTG":7127109, "CAGCA":4806513, "CAGCT":5883433, "CAGCC":2289459, "CAGCG":1510476, "CAGGA":5213597, "CAGGT":4883385, "CAGGC":2422743, "CAGGG":4911560, "CTAAA":3688029, "CTAAT":2538382, "CTAAC":2072749, "CTAAG":1854052, "CTATA":3866972, "CTATT":3105164, "CTATC":1568308, "CTATG":3224698, "CTACA":3498808, "CTACT":2334246, "CTACC":1486919, "CTACG":862148, "CTAGA":3089310, "CTAGT":3305767, 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"CCACA":12220484, "CCACT":8412019, "CCACC":7141196, "CCACG":1820360, "CCAGA":6099882, "CCAGT":4536367, "CCAGC":4081020, "CCAGG":3252226, "CCTAA":3978111, "CCTAT":3724695, "CCTAC":8964406, "CCTAG":2585921, "CCTTA":3706750, "CCTTT":5650598, "CCTTC":6173256, "CCTTG":2939117, "CCTCA":6872783, "CCTCT":13152858, "CCTCC":9171492, "CCTCG":1355847, "CCTGA":3963736, "CCTGT":6057563, "CCTGC":4135659, "CCTGG":3346413, "CCCAA":5796210, "CCCAT":4591864, "CCCAC":7016142, "CCCAG":4935179, "CCCTA":6301465, "CCCTT":4648397, "CCCTC":9082006, "CCCTG":4774641, "CCCCA":7118739, "CCCCT":6708130, "CCCCC":6965718, "CCCCG":1753900, "CCCGA":995187, "CCCGT":1265703, "CCCGC":1323517, "CCCGG":1003196, "CCGAA":811698, "CCGAT":1061844, "CCGAC":1219465, "CCGAG":1064996, "CCGTA":1101336, "CCGTT":1275381, "CCGTC":1564104, "CCGTG":1091213, "CCGCA":1133544, "CCGCT":1414032, "CCGCC":1524191, "CCGCG":486789, "CCGGA":802275, "CCGGT":902108, "CCGGC":923756, "CCGGG":913273, "CGAAA":1034906, "CGAAT":769996, "CGAAC":842372, 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"GGCCG":881072, "GGCGA":664317, "GGCGT":616176, "GGCGC":753953, "GGCGG":1089642, "GGGAA":4607426, "GGGAT":1606075, "GGGAC":1681646, "GGGAG":2334986, "GGGTA":1421205, "GGGTT":1655263, "GGGTC":1335772, "GGGTG":2084621, "GGGCA":1556648, "GGGCT":1422415, "GGGCC":1454325, "GGGCG":929846, "GGGGA":1752749, "GGGGT":1753872, "GGGGC":1440630, "GGGGG":17815597 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 41304507, "total_bases": 3378727860, "q20_bases": 3282453051, "q30_bases": 3118196553, "total_cycles": 86, "quality_curves": { 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"C":[36.4145,36.4161,36.3848,36.3788,36.3724,36.3592,36.3442,36.3337,36.3137,36.3039,36.2525,36.2454,36.2525,36.2373,36.2204,36.2068,36.1929,36.1962,36.1795,36.1726,36.1637,36.1641,36.1461,36.1272,36.1199,36.1129,36.0894,36.0841,36.0473,36.054,36.076,36.0521,36.0404,36.0382,36.0283,36.026,35.9819,35.9914,35.9516,35.9596,35.9046,35.9184,35.928,35.9101,35.8874,35.8828,35.8491,35.8494,35.8098,35.825,35.774,35.7899,35.7952,35.7928,35.8018,35.7944,35.7665,35.7557,35.7298,35.7058,35.6885,35.6645,35.6642,35.6641,35.6678,35.6115,35.6216,35.6128,35.5881,35.5811,35.5462,35.5347,35.5314,35.5393,35.4973,35.5141,35.5186,35.4979,35.4953,35.4631,35.4412,35.4176,35.4287,35.377,35.3935,35.2524], 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"CATGG":2652379, "CACAA":7600850, "CACAT":7077525, "CACAC":29255790, "CACAG":7972283, "CACTA":7256701, "CACTT":4072873, "CACTC":6428028, "CACTG":5927910, "CACCA":7208921, "CACCT":5165046, "CACCC":4158288, "CACCG":1304203, "CACGA":977390, "CACGT":1365353, "CACGC":1632878, "CACGG":925833, "CAGAA":3990441, "CAGAT":3387103, "CAGAC":5471833, "CAGAG":4426806, "CAGTA":4458737, "CAGTT":3871816, "CAGTC":4096875, "CAGTG":3533411, "CAGCA":4671209, "CAGCT":5998312, "CAGCC":3501679, "CAGCG":994036, "CAGGA":3155904, "CAGGT":2808820, "CAGGC":2292644, "CAGGG":2003247, "CTAAA":4081529, "CTAAT":3064671, "CTAAC":4600232, "CTAAG":1312300, "CTATA":5487196, "CTATT":4387421, "CTATC":5333722, "CTATG":2739255, "CTACA":7752706, "CTACT":10581956, "CTACC":7546636, "CTACG":1076222, "CTAGA":2247829, "CTAGT":2651417, "CTAGC":2215535, "CTAGG":1524981, "CTTAA":2692770, "CTTAT":3261550, "CTTAC":2803674, "CTTAG":1490074, "CTTTA":4311536, "CTTTT":5544152, "CTTTC":6372937, "CTTTG":3728044, "CTTCA":5496304, "CTTCT":7231260, "CTTCC":4743052, "CTTCG":842334, "CTTGA":2380227, "CTTGT":3346507, "CTTGC":1879388, "CTTGG":1521270, "CTCAA":4406119, "CTCAT":4851487, "CTCAC":6368416, "CTCAG":3598730, "CTCTA":7408531, "CTCTT":7683935, "CTCTC":32917967, "CTCTG":8646558, "CTCCA":6955132, "CTCCT":9573308, "CTCCC":7099638, "CTCCG":1197744, "CTCGA":707268, "CTCGT":1143862, "CTCGC":1703538, "CTCGG":679419, "CTGAA":3772688, "CTGAT":3509241, "CTGAC":4392530, "CTGAG":2664560, "CTGTA":5616043, "CTGTT":6333029, "CTGTC":9490885, "CTGTG":4560575, "CTGCA":4392958, "CTGCT":6276745, "CTGCC":3861967, "CTGCG":776699, "CTGGA":3253402, "CTGGT":3409179, "CTGGC":2240132, "CTGGG":1865043, "CCAAA":4325206, "CCAAT":3302626, "CCAAC":5866078, "CCAAG":2442989, "CCATA":4018129, "CCATT":4490666, "CCATC":5415030, "CCATG":3339081, "CCACA":9747291, "CCACT":6740486, "CCACC":5446904, "CCACG":1413753, "CCAGA":3656093, "CCAGT":3629089, "CCAGC":3235103, "CCAGG":2585121, "CCTAA":3150810, "CCTAT":3029734, "CCTAC":6558771, "CCTAG":1987799, "CCTTA":3008175, "CCTTT":4642931, "CCTTC":4990022, "CCTTG":2390277, "CCTCA":5437580, "CCTCT":10427488, "CCTCC":7236443, "CCTCG":1053402, "CCTGA":3157845, "CCTGT":4806684, "CCTGC":3316339, "CCTGG":2658831, "CCCAA":4543607, "CCCAT":3669325, "CCCAC":5310299, "CCCAG":3510010, "CCCTA":5090741, "CCCTT":3793902, "CCCTC":7178633, "CCCTG":3842239, "CCCCA":5312390, "CCCCT":5308364, "CCCCC":3916914, "CCCCG":1141981, "CCCGA":714699, "CCCGT":980134, "CCCGC":830652, "CCCGG":663039, "CCGAA":625877, "CCGAT":842805, "CCGAC":909454, "CCGAG":745654, "CCGTA":821862, "CCGTT":1025788, "CCGTC":1220371, "CCGTG":858338, "CCGCA":841147, "CCGCT":1086966, "CCGCC":1037111, "CCGCG":266574, "CCGGA":588445, "CCGGT":685425, "CCGGC":650356, "CCGGG":568117, "CGAAA":818595, "CGAAT":615840, "CGAAC":663465, "CGAAG":541560, "CGATA":762982, "CGATT":804361, "CGATC":718745, "CGATG":810837, "CGACA":1436469, "CGACT":829333, "CGACC":1045939, "CGACG":408968, "CGAGA":958799, "CGAGT":557217, "CGAGC":580670, "CGAGG":653746, "CGTAA":765951, "CGTAT":743914, "CGTAC":744411, "CGTAG":728853, "CGTTA":1055172, "CGTTT":1454900, "CGTTC":1074851, "CGTTG":996425, "CGTCA":1283745, "CGTCT":1518228, "CGTCC":1000194, "CGTCG":344633, "CGTGA":722857, "CGTGT":1163426, "CGTGC":715065, "CGTGG":701940, "CGCAA":878752, "CGCAT":728168, "CGCAC":1184103, "CGCAG":835067, "CGCTA":937865, "CGCTT":819977, "CGCTC":2009998, "CGCTG":1279738, "CGCCA":1057215, "CGCCT":882048, "CGCCC":1070284, "CGCCG":352113, "CGCGA":207766, "CGCGT":233962, "CGCGC":396289, "CGCGG":239971, "CGGAA":859976, "CGGAT":520853, "CGGAC":674906, "CGGAG":771513, "CGGTA":689516, "CGGTT":670350, "CGGTC":710856, "CGGTG":789096, "CGGCA":774300, "CGGCT":760401, "CGGCC":774505, "CGGCG":324466, "CGGGA":577274, "CGGGT":417575, "CGGGC":433811, "CGGGG":489708, "GAAAA":3836165, "GAAAT":3514369, "GAAAC":3047154, "GAAAG":2468186, "GAATA":2566732, "GAATT":2348141, "GAATC":1679374, "GAATG":2512863, "GAACA":3780978, "GAACT":2139723, "GAACC":1975021, "GAACG":915780, "GAAGA":2864133, "GAAGT":2102146, "GAAGC":1652114, "GAAGG":1833224, "GATAA":2013360, "GATAT":2483325, "GATAC":1878977, "GATAG":1385652, "GATTA":2125883, "GATTT":3174143, "GATTC":1794916, "GATTG":1932146, "GATCA":2275277, "GATCT":2284966, "GATCC":1591160, "GATCG":815494, "GATGA":2700269, "GATGT":2917415, "GATGC":1750525, "GATGG":1868412, "GACAA":3982230, "GACAT":3603789, "GACAC":4517294, "GACAG":4559888, "GACTA":2237023, "GACTT":2232169, "GACTC":2366879, "GACTG":2902591, "GACCA":3465965, "GACCT":2265878, "GACCC":2212332, "GACCG":675754, "GACGA":967941, "GACGT":827939, "GACGC":673144, "GACGG":728430, "GAGAA":3134629, "GAGAT":2295312, "GAGAC":3196794, "GAGAG":4944424, "GAGTA":1571868, "GAGTT":1793450, "GAGTC":1487082, "GAGTG":1856798, "GAGCA":1990850, "GAGCT":1932265, "GAGCC":1623634, "GAGCG":721957, "GAGGA":2663217, "GAGGT":1682084, "GAGGC":1469480, "GAGGG":1561221, "GTAAA":3292738, "GTAAT":2822068, "GTAAC":2313488, "GTAAG":1307698, "GTATA":3009075, "GTATT":3576947, "GTATC":2233801, "GTATG":2189969, "GTACA":3565728, "GTACT":2671667, "GTACC":2142885, "GTACG":600183, "GTAGA":2127983, "GTAGT":1932306, "GTAGC":1686182, "GTAGG":1310666, "GTTAA":2658466, "GTTAT":2937936, "GTTAC":2632459, "GTTAG":1723269, "GTTTA":3401162, "GTTTT":5348812, "GTTTC":3422572, "GTTTG":3491916, "GTTCA":3166361, "GTTCT":3751204, "GTTCC":2561733, "GTTCG":531633, "GTTGA":2604027, "GTTGT":3465495, "GTTGC":2016901, "GTTGG":1641093, "GTCAA":2872999, "GTCAT":3673042, "GTCAC":2926996, "GTCAG":2731514, "GTCTA":2628499, "GTCTT":3345996, "GTCTC":6139406, "GTCTG":4829651, "GTCCA":2836844, "GTCCT":3209148, "GTCCC":2453628, "GTCCG":626256, "GTCGA":583059, "GTCGT":804682, "GTCGC":578039, "GTCGG":513079, "GTGAA":2558269, "GTGAT":2211563, "GTGAC":2063165, "GTGAG":1664530, "GTGTA":2736948, "GTGTT":4299641, "GTGTC":3090333, "GTGTG":8071101, "GTGCA":2205757, "GTGCT":2150818, "GTGCC":1572044, "GTGCG":609148, "GTGGA":2165070, "GTGGT":1994614, "GTGGC":1524386, "GTGGG":1262860, "GCAAA":2528495, "GCAAT":2003061, "GCAAC":2569029, "GCAAG":1640845, "GCATA":1835965, "GCATT":2753103, "GCATC":2045153, "GCATG":1966785, "GCACA":3496134, "GCACT":2274596, "GCACC":2086971, "GCACG":710198, "GCAGA":2439734, "GCAGT":2297486, "GCAGC":2793958, "GCAGG":1852338, "GCTAA":1975482, "GCTAT":2199983, "GCTAC":2985391, "GCTAG":1549299, "GCTTA":1283959, "GCTTT":3134033, "GCTTC":2478455, "GCTTG":1470091, "GCTCA":2341087, "GCTCT":4299536, "GCTCC":3836850, "GCTCG":718111, "GCTGA":2419069, "GCTGT":3584808, "GCTGC":4032942, "GCTGG":2194782, "GCCAA":2432516, "GCCAT":2755745, "GCCAC":2671163, "GCCAG":2007532, "GCCTA":1854828, "GCCTT":2406811, "GCCTC":2554876, "GCCTG":2053174, "GCCCA":2299149, "GCCCT":2229978, "GCCCC":2026197, "GCCCG":539937, "GCCGA":604959, "GCCGT":647239, "GCCGC":653424, "GCCGG":526997, "GCGAA":521939, "GCGAT":481083, "GCGAC":513409, "GCGAG":495559, "GCGTA":425848, "GCGTT":719059, "GCGTC":613996, "GCGTG":649077, "GCGCA":604929, "GCGCT":754732, "GCGCC":538040, "GCGCG":269365, "GCGGA":408392, "GCGGT":505264, "GCGGC":509467, "GCGGG":362337, "GGAAA":2899862, "GGAAT":1915709, "GGAAC":1907182, "GGAAG":1934485, "GGATA":1454226, "GGATT":1781121, "GGATC":1357927, "GGATG":1766881, "GGACA":3189509, "GGACT":1809040, "GGACC":1523468, "GGACG":658506, "GGAGA":2797053, "GGAGT":1578142, "GGAGC":1458434, "GGAGG":1976814, "GGTAA":1619359, "GGTAT":1638983, "GGTAC":1603502, "GGTAG":1278453, "GGTTA":1639198, "GGTTT":2327316, "GGTTC":1404295, "GGTTG":1514722, "GGTCA":2232418, "GGTCT":2180012, "GGTCC":1353134, "GGTCG":451776, "GGTGA":1775114, "GGTGT":2293756, "GGTGC":1220056, "GGTGG":1458331, "GGCAA":1627122, "GGCAT":1603280, "GGCAC":1515481, "GGCAG":1878987, "GGCTA":1810177, "GGCTT":1600601, "GGCTC":1611975, "GGCTG":2025453, "GGCCA":2097734, "GGCCT":1879898, "GGCCC":1366062, "GGCCG":588658, "GGCGA":513377, "GGCGT":450435, "GGCGC":471071, "GGCGG":501428, "GGGAA":1708163, "GGGAT":1270733, "GGGAC":1288274, "GGGAG":1571016, "GGGTA":1098626, "GGGTT":1262216, "GGGTC":990318, "GGGTG":1209257, "GGGCA":1197688, "GGGCT":1109117, "GGGCC":1057597, "GGGCG":364522, "GGGGA":1242089, "GGGGT":966005, "GGGGC":787769, "GGGGG":1101394 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213504_sample_1703675_S127_L002_R1_001.fastq.gz --in2 lib_213504_sample_1703675_S127_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213504_sample_1703675_S127_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213504_sample_1703675_S127_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213504_sample_1703675_S127_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213504_sample_1703675_S127_L002_R2_001.fastp-trim.fq.gz " }