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"GC":[0.676878,0.518542,0.421072,0.44256,0.400638,0.409768,0.339063,0.416247,0.47129,0.349013,0.498721,0.488099,0.428281,0.417621,0.418564,0.428058,0.43789,0.422334,0.439388,0.420977,0.438339,0.423275,0.427866,0.429019,0.427873,0.433228,0.434414,0.424036,0.438924,0.421065,0.435601,0.421568,0.434433,0.425107,0.437444,0.425216,0.437036,0.425915,0.438,0.429741,0.430496,0.422781,0.437531,0.424642,0.426695,0.434024,0.432185,0.429742,0.439182,0.425141,0.428457,0.423875,0.438924,0.433997,0.438412,0.434626,0.433155,0.436063,0.436922,0.427818,0.440813,0.431764,0.440602,0.437099,0.440524,0.428848,0.444498,0.433684,0.435657,0.432555,0.441642,0.437248,0.443601,0.440966,0.444342,0.44226,0.446495,0.441072,0.447643,0.44281,0.446418,0.441353,0.44626,0.442405,0.447281,0.444812,0.447978,0.445865,0.452382,0.44528,0.452494,0.448111,0.450618,0.45034,0.453085,0.450101,0.453652,0.45194,0.455167,0.451831,0.460033] }, "kmer_count": { "AAAAA":10300288, "AAAAT":6797442, "AAAAC":4747561, "AAAAG":5937928, 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"CCAGC":1488895, "CCAGG":2251042, "CCTAA":1276220, "CCTAT":1236845, "CCTAC":1063620, "CCTAG":1341911, "CCTTA":1047108, "CCTTT":1879806, "CCTTC":1327786, "CCTTG":1760684, "CCTCA":1574415, "CCTCT":1935492, "CCTCC":1486967, "CCTCG":531353, "CCTGA":1768640, "CCTGT":2618427, "CCTGC":1481462, "CCTGG":2121907, "CCCAA":1241287, "CCCAT":1226395, "CCCAC":991549, "CCCAG":1574930, "CCCTA":795536, "CCCTT":964430, "CCCTC":1053297, "CCCTG":1491058, "CCCCA":1001652, "CCCCT":850919, "CCCCC":682869, "CCCCG":431097, "CCCGA":430459, "CCCGT":410484, "CCCGC":294711, "CCCGG":419338, "CCGAA":641337, "CCGAT":657689, "CCGAC":458927, "CCGAG":677030, "CCGTA":570592, "CCGTT":710594, "CCGTC":931235, "CCGTG":854965, "CCGCA":412796, "CCGCT":446200, "CCGCC":403228, "CCGCG":172001, "CCGGA":484214, "CCGGT":714045, "CCGGC":449704, "CCGGG":680044, "CGAAA":868149, "CGAAT":679920, "CGAAC":467801, "CGAAG":793589, "CGATA":850475, "CGATT":844008, "CGATC":647965, "CGATG":1126242, "CGACA":923161, "CGACT":566570, "CGACC":377392, "CGACG":271732, "CGAGA":1883928, "CGAGT":771376, "CGAGC":578991, "CGAGG":1115166, "CGTAA":794414, "CGTAT":1235796, "CGTAC":524872, "CGTAG":890209, "CGTTA":782018, "CGTTT":1252480, "CGTTC":701843, "CGTTG":1058546, "CGTCA":915031, "CGTCT":4935921, "CGTCC":502292, "CGTCG":460207, "CGTGA":1003374, "CGTGT":1481049, "CGTGC":576038, "CGTGG":1091770, "CGCAA":581214, "CGCAT":496818, "CGCAC":521298, "CGCAG":728505, "CGCTA":472915, "CGCTT":426268, "CGCTC":494513, "CGCTG":855348, "CGCCA":527992, "CGCCT":542515, "CGCCC":292514, "CGCCG":303273, "CGCGA":220533, "CGCGT":407558, "CGCGC":216648, "CGCGG":233228, "CGGAA":6858130, "CGGAT":716204, "CGGAC":518986, "CGGAG":1256880, "CGGTA":863383, "CGGTT":823962, "CGGTC":561432, "CGGTG":1067879, "CGGCA":754814, "CGGCT":650194, "CGGCC":462989, "CGGCG":409941, "CGGGA":882320, "CGGGT":716593, "CGGGC":491261, "CGGGG":1250754, "GAAAA":6036335, "GAAAT":4138890, "GAAAC":3348980, "GAAAG":6939915, "GAATA":4191201, "GAATT":2346782, "GAATC":1664301, "GAATG":4414570, 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"GTGGT":5492185, "GTGGC":2250452, "GTGGG":4575274, "GCAAA":2996918, "GCAAT":1941086, "GCAAC":2798535, "GCAAG":1881134, "GCATA":1759103, "GCATT":2508139, "GCATC":1515747, "GCATG":2509510, "GCACA":7135181, "GCACT":1747133, "GCACC":996600, "GCACG":696955, "GCAGA":4267668, "GCAGT":3289495, "GCAGC":2299039, "GCAGG":3046657, "GCTAA":1887306, "GCTAT":1981727, "GCTAC":1483300, "GCTAG":2040623, "GCTTA":1103059, "GCTTT":2125254, "GCTTC":1343698, "GCTTG":1910842, "GCTCA":1790653, "GCTCT":1736589, "GCTCC":1107069, "GCTCG":484703, "GCTGA":2690582, "GCTGT":3682168, "GCTGC":2232190, "GCTGG":2866464, "GCCAA":1664930, "GCCAT":1897743, "GCCAC":1292702, "GCCAG":2177762, "GCCTA":1403361, "GCCTT":1505751, "GCCTC":1189818, "GCCTG":1933903, "GCCCA":1085228, "GCCCT":997619, "GCCCC":647305, "GCCCG":367310, "GCCGA":629470, "GCCGT":1110784, "GCCGC":388056, "GCCGG":735089, "GCGAA":653035, "GCGAT":864615, "GCGAC":506900, "GCGAG":1634169, "GCGTA":746802, "GCGTT":860799, "GCGTC":579158, "GCGTG":1383171, 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"GGGAG":8261694, "GGGTA":3554897, "GGGTT":4449476, "GGGTC":2167970, "GGGTG":4460457, "GGGCA":2303667, "GGGCT":2509655, "GGGCC":1273231, "GGGCG":1099067, "GGGGA":5958586, "GGGGT":5177008, "GGGGC":2210449, "GGGGG":13875902 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 29685935, "total_bases": 2998279435, "q20_bases": 2853950006, "q30_bases": 2684020238, "total_cycles": 101, "quality_curves": { 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"T":[35.6759,35.8215,35.7491,35.7431,35.8717,35.7649,35.8087,35.8251,35.7691,35.77,35.7152,35.7512,35.7785,35.7506,35.7584,35.748,35.7116,35.7099,35.6888,35.6313,35.6347,35.5751,35.5717,35.5505,35.5402,35.5432,35.5181,35.4773,35.4672,35.4299,35.4006,35.411,35.3532,35.3197,35.2592,35.2536,35.2505,35.1915,35.1713,35.1705,35.1567,35.1829,35.1679,35.142,35.1127,35.085,34.995,35.0072,34.9373,34.8815,34.9152,34.865,34.8771,34.9261,34.9043,34.8612,34.8593,34.8684,34.8184,34.8247,34.8074,34.8419,34.8563,34.8179,34.7818,34.7531,34.7195,34.7504,34.6675,34.631,34.5889,34.5744,34.5099,34.4683,34.4377,34.3965,34.3532,34.3618,34.3467,34.3209,34.3324,34.2571,34.2778,34.2184,34.2257,34.1944,34.1547,34.1423,34.0958,34.07,34.1232,34.0884,34.0953,34.0492,34.0226,33.9546,33.9779,33.9538,33.9502,33.9271,33.561], 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"TGTGA":3012755, "TGTGT":7893361, "TGTGC":2395794, "TGTGG":2775329, "TGCAA":2496842, "TGCAT":3133330, "TGCAC":2656695, "TGCAG":3016329, "TGCTA":2592841, "TGCTT":2976564, "TGCTC":2904930, "TGCTG":3463103, "TGCCA":2951302, "TGCCT":2970122, "TGCCC":2121418, "TGCCG":765155, "TGCGA":523569, "TGCGT":837442, "TGCGC":598426, "TGCGG":484051, "TGGAA":2695643, "TGGAT":2057604, "TGGAC":2177864, "TGGAG":2498573, "TGGTA":1818552, "TGGTT":2627193, "TGGTC":2359712, "TGGTG":2592474, "TGGCA":2217143, "TGGCT":2435522, "TGGCC":1938520, "TGGCG":1564361, "TGGGA":1722512, "TGGGT":1814884, "TGGGC":1167513, "TGGGG":2121748, "CAAAA":4530314, "CAAAT":3738679, "CAAAC":3998993, "CAAAG":2638853, "CAATA":3451406, "CAATT":2958977, "CAATC":2610475, "CAATG":3145962, "CAACA":5945757, "CAACT":3180066, "CAACC":3700279, "CAACG":1102439, "CAAGA":2798614, "CAAGT":2041542, "CAAGC":1890777, "CAAGG":1894319, "CATAA":3150328, "CATAT":3802693, "CATAC":4039394, "CATAG":2283485, "CATTA":4672996, "CATTT":5867302, "CATTC":4060561, 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"CTTAC":2538684, "CTTAG":1362177, "CTTTA":4172149, "CTTTT":5070394, "CTTTC":5990242, "CTTTG":3494657, "CTTCA":4699087, "CTTCT":6315934, "CTTCC":4644716, "CTTCG":826527, "CTTGA":2153482, "CTTGT":2783431, "CTTGC":1732792, "CTTGG":1397904, "CTCAA":3625434, "CTCAT":4383655, "CTCAC":5756835, "CTCAG":3471895, "CTCTA":6909723, "CTCTT":7396198, "CTCTC":30150149, "CTCTG":7886670, "CTCCA":6433135, "CTCCT":9107753, "CTCCC":6859289, "CTCCG":1180651, "CTCGA":706951, "CTCGT":1129233, "CTCGC":1534798, "CTCGG":1030637, "CTGAA":3318606, "CTGAT":3053146, "CTGAC":3885245, "CTGAG":2570844, "CTGTA":5382802, "CTGTT":6170287, "CTGTC":8060487, "CTGTG":4096023, "CTGCA":3643948, "CTGCT":4447791, "CTGCC":3371868, "CTGCG":732433, "CTGGA":2602570, "CTGGT":2597641, "CTGGC":2154811, "CTGGG":1681670, "CCAAA":3741033, "CCAAT":2822250, "CCAAC":3650332, "CCAAG":2205943, "CCATA":3397456, "CCATT":4137857, "CCATC":5264407, "CCATG":3150025, "CCACA":5671292, "CCACT":5154870, "CCACC":5165190, "CCACG":1171545, "CCAGA":4833712, "CCAGT":3293247, "CCAGC":2932016, "CCAGG":2328930, "CCTAA":2754755, "CCTAT":3029808, "CCTAC":4174310, "CCTAG":1848919, "CCTTA":2623630, "CCTTT":4406528, "CCTTC":4820275, "CCTTG":2168906, "CCTCA":4802897, "CCTCT":10658349, "CCTCC":7595510, "CCTCG":1076366, "CCTGA":2890209, "CCTGT":4732707, "CCTGC":2959589, "CCTGG":2409378, "CCCAA":3169666, "CCCAT":3627016, "CCCAC":4428455, "CCCAG":3921632, "CCCTA":4001373, "CCCTT":3689294, "CCCTC":7569077, "CCCTG":3742205, "CCCCA":4789064, "CCCCT":5387027, "CCCCC":5907916, "CCCCG":1390208, "CCCGA":718539, "CCCGT":988155, "CCCGC":1040591, "CCCGG":801129, "CCGAA":581237, "CCGAT":759634, "CCGAC":803294, "CCGAG":798975, "CCGTA":848712, "CCGTT":972110, "CCGTC":1188826, "CCGTG":785087, "CCGCA":808892, "CCGCT":1005297, "CCGCC":1185296, "CCGCG":360388, "CCGGA":605543, "CCGGT":705960, "CCGGC":712031, "CCGGG":709580, "CGAAA":714401, "CGAAT":586959, "CGAAC":589387, "CGAAG":481532, "CGATA":686790, "CGATT":724906, "CGATC":675218, "CGATG":748509, "CGACA":1080398, "CGACT":776145, "CGACC":908638, "CGACG":372355, "CGAGA":1080474, "CGAGT":527081, "CGAGC":554716, "CGAGG":609867, "CGTAA":657215, "CGTAT":762397, "CGTAC":695830, "CGTAG":685458, "CGTTA":954052, "CGTTT":1358517, "CGTTC":1044138, "CGTTG":924209, "CGTCA":1156094, "CGTCT":1407500, "CGTCC":964234, "CGTCG":4506643, "CGTGA":688151, "CGTGT":4536286, "CGTGC":682037, "CGTGG":857444, "CGCAA":732912, "CGCAT":681993, "CGCAC":972026, "CGCAG":897732, "CGCTA":859564, "CGCTT":768960, "CGCTC":1776226, "CGCTG":1135642, "CGCCA":1044082, "CGCCT":878053, "CGCCC":1225442, "CGCCG":558516, "CGCGA":215796, "CGCGT":249336, "CGCGC":458465, "CGCGG":377045, "CGGAA":6366103, "CGGAT":506011, "CGGAC":651894, "CGGAG":777051, "CGGTA":659485, "CGGTT":624385, "CGGTC":697133, "CGGTG":1873224, "CGGCA":733410, "CGGCT":734799, "CGGCC":812974, "CGGCG":436165, "CGGGA":571788, "CGGGT":478425, "CGGGC":529428, "CGGGG":963443, "GAAAA":3197874, "GAAAT":2905872, "GAAAC":2503329, "GAAAG":4418621, "GAATA":2237535, "GAATT":1992409, "GAATC":1464895, "GAATG":2167440, "GAACA":3076323, "GAACT":1846885, "GAACC":1753640, "GAACG":794037, "GAAGA":7648453, "GAAGT":1809940, "GAAGC":1377609, "GAAGG":1499455, "GATAA":1724373, "GATAT":2120909, "GATAC":1532055, "GATAG":1226373, "GATTA":1831264, "GATTT":2769023, "GATTC":1612156, "GATTG":1712908, "GATCA":1942850, "GATCT":2434878, "GATCC":1360261, "GATCG":6955984, "GATGA":2333597, "GATGT":2514182, "GATGC":1495237, "GATGG":1590682, "GACAA":2731894, "GACAT":3069315, "GACAC":3217328, "GACAG":3176637, "GACTA":2039083, "GACTT":1970859, "GACTC":2086603, "GACTG":2542097, "GACCA":2802332, "GACCT":1993915, "GACCC":2040364, "GACCG":582664, "GACGA":876694, "GACGT":760667, "GACGC":617915, "GACGG":637869, "GAGAA":2503591, "GAGAT":3042818, "GAGAC":2176067, "GAGAG":3284739, "GAGTA":1390811, "GAGTT":1624183, "GAGTC":1334445, "GAGTG":3649736, "GAGCA":1783641, "GAGCT":1721469, "GAGCC":1510448, "GAGCG":5360994, "GAGGA":2188125, "GAGGT":1485481, "GAGGC":1306356, "GAGGG":1461244, "GTAAA":2805073, "GTAAT":2345990, "GTAAC":1953856, "GTAAG":1177213, "GTATA":2777059, "GTATT":3191876, "GTATC":1939483, "GTATG":2020560, "GTACA":2966732, "GTACT":2145729, "GTACC":1882023, "GTACG":533971, "GTAGA":2590350, "GTAGT":1818552, "GTAGC":1517371, "GTAGG":4177361, "GTTAA":2358264, "GTTAT":2668711, "GTTAC":2248971, "GTTAG":1586252, "GTTTA":4635184, "GTTTT":4771914, "GTTTC":3256361, "GTTTG":3123465, "GTTCA":2807818, "GTTCT":3692942, "GTTCC":2259935, "GTTCG":489254, "GTTGA":2383189, "GTTGT":3317641, "GTTGC":1637465, "GTTGG":1696100, "GTCAA":2418152, "GTCAT":3092575, "GTCAC":2742816, "GTCAG":2546158, "GTCTA":2405106, "GTCTT":2891616, "GTCTC":5162527, "GTCTG":3852945, "GTCCA":2612486, "GTCCT":3063252, "GTCCC":2464774, "GTCCG":530656, "GTCGA":531757, "GTCGT":4674096, "GTCGC":727806, "GTCGG":617417, "GTGAA":2202369, "GTGAT":1943863, "GTGAC":1821465, "GTGAG":1538652, "GTGTA":6525199, "GTGTT":5628891, "GTGTC":2605414, "GTGTG":5502442, "GTGCA":1998529, "GTGCT":1942545, "GTGCC":1495802, "GTGCG":586654, "GTGGA":2050872, "GTGGT":2336741, "GTGGC":1405831, "GTGGG":2187106, "GCAAA":2149726, "GCAAT":1758605, "GCAAC":1832946, "GCAAG":1412274, "GCATA":1669974, "GCATT":2447100, "GCATC":1955645, "GCATG":1840030, "GCACA":2925311, "GCACT":2054010, "GCACC":1867933, "GCACG":625661, "GCAGA":2676823, "GCAGT":2042302, "GCAGC":2311114, "GCAGG":1705500, "GCTAA":1712595, "GCTAT":1991369, "GCTAC":2280467, "GCTAG":1428200, "GCTTA":1137049, "GCTTT":2854751, "GCTTC":2099482, "GCTTG":1313288, "GCTCA":2127097, "GCTCT":3739122, "GCTCC":2176892, "GCTCG":614413, "GCTGA":2140885, "GCTGT":3267442, "GCTGC":2392562, "GCTGG":1725898, "GCCAA":2186351, "GCCAT":2544611, "GCCAC":2359002, "GCCAG":2115778, "GCCTA":1677240, "GCCTT":2187326, "GCCTC":2546524, "GCCTG":1908944, "GCCCA":2213250, "GCCCT":2250756, "GCCCC":2181578, "GCCCG":592835, "GCCGA":606482, "GCCGT":730096, "GCCGC":698470, "GCCGG":637335, "GCGAA":455878, "GCGAT":434479, "GCGAC":485345, "GCGAG":487893, "GCGTA":401781, "GCGTT":686945, "GCGTC":4926400, "GCGTG":660236, "GCGCA":583779, "GCGCT":707973, "GCGCC":609837, "GCGCG":376989, "GCGGA":403719, "GCGGT":1389027, "GCGGC":587656, "GCGGG":761288, "GGAAA":4978693, "GGAAT":1708586, "GGAAC":1570747, "GGAAG":7072172, "GGATA":1239513, "GGATT":1596374, "GGATC":1216332, "GGATG":1544469, "GGACA":2465763, "GGACT":1603133, "GGACC":1358110, "GGACG":611876, "GGAGA":2433925, "GGAGT":1423393, "GGAGC":1349081, "GGAGG":1934983, "GGTAA":1452733, "GGTAT":1492111, "GGTAC":1285391, "GGTAG":1174476, "GGTTA":1463606, "GGTTT":2177190, "GGTTC":1233126, "GGTTG":1566209, "GGTCA":1965457, "GGTCT":1800555, "GGTCC":1234765, "GGTCG":648056, "GGTGA":1525357, "GGTGT":3145889, "GGTGC":1101290, "GGTGG":2583542, "GGCAA":1403315, "GGCAT":1472224, "GGCAC":1339011, "GGCAG":1775218, "GGCTA":1607240, "GGCTT":1463990, "GGCTC":1507448, "GGCTG":1896556, "GGCCA":1955474, "GGCCT":1714827, "GGCCC":1370797, "GGCCG":622260, "GGCGA":473739, "GGCGT":478361, "GGCGC":569040, "GGCGG":1767116, "GGGAA":4158371, "GGGAT":1159719, "GGGAC":1117818, "GGGAG":1404169, "GGGTA":1084118, "GGGTT":1350603, "GGGTC":932300, "GGGTG":2146916, "GGGCA":1049419, "GGGCT":1030729, "GGGCC":1107441, "GGGCG":710705, "GGGGA":1158096, "GGGGT":1905892, "GGGGC":1052896, "GGGGG":24530742 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 28669865, "total_bases": 2290339480, "q20_bases": 2230673783, "q30_bases": 2125484435, "total_cycles": 86, "quality_curves": { 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"T":[35.9618,35.9479,35.9551,35.9393,35.9361,35.927,35.9157,35.8889,35.8645,35.8484,35.774,35.7564,35.7569,35.7664,35.7516,35.7251,35.7421,35.719,35.7057,35.7195,35.69,35.679,35.6682,35.6549,35.6578,35.6452,35.6466,35.6369,35.6074,35.6127,35.6009,35.5796,35.5736,35.5922,35.5489,35.5544,35.5584,35.5006,35.5367,35.4896,35.559,35.4649,35.5033,35.4628,35.4463,35.5196,35.4302,35.41,35.46,35.386,35.3356,35.3184,35.3656,35.3359,35.352,35.3824,35.3742,35.3679,35.3427,35.3258,35.2786,35.2745,35.2501,35.2558,35.2459,35.2183,35.1678,35.2152,35.22,35.1983,35.1563,35.1466,35.1367,35.1628,35.0951,35.1462,35.1465,35.1547,35.1181,35.0981,35.0546,35.0847,35.0491,35.0206,35.0035,34.9611], "C":[36.3835,36.3855,36.3719,36.3726,36.3563,36.3512,36.3311,36.326,36.3063,36.2941,36.2585,36.2566,36.2624,36.2424,36.2266,36.2125,36.2098,36.2086,36.2036,36.1939,36.1826,36.187,36.169,36.1566,36.1445,36.1387,36.1194,36.1097,36.0877,36.0981,36.116,36.0922,36.075,36.0836,36.0747,36.0825,36.0308,36.0447,35.9851,36.0088,35.9738,35.992,35.9692,35.9837,35.9573,35.9484,35.9292,35.9319,35.8778,35.9078,35.8723,35.8861,35.8895,35.8969,35.8966,35.8919,35.8708,35.8457,35.8297,35.8137,35.793,35.7735,35.7701,35.78,35.7834,35.7347,35.7343,35.739,35.7123,35.7041,35.6742,35.6691,35.6677,35.6793,35.6398,35.6511,35.6548,35.6427,35.6236,35.6084,35.5793,35.5628,35.5665,35.5229,35.5431,35.4217], "G":[35.9972,35.9989,36.0074,36.0176,36.0137,36.0105,35.9885,35.9913,35.966,35.9601,35.8862,35.8706,35.888,35.8739,35.8581,35.843,35.8517,35.8386,35.8299,35.8246,35.7951,35.7944,35.7852,35.7707,35.7659,35.7657,35.7241,35.7137,35.6924,35.6809,35.696,35.616,35.6388,35.6811,35.6286,35.6418,35.608,35.6404,35.5782,35.5596,35.5831,35.5361,35.5562,35.5516,35.4514,35.5199,35.5093,35.5086,35.4634,35.4855,35.3884,35.4858,35.4491,35.4041,35.4061,35.4272,35.4479,35.4412,35.4147,35.3742,35.355,35.3502,35.3613,35.3382,35.3254,35.2747,35.2483,35.2767,35.2445,35.1983,35.1708,35.1328,35.1623,35.1739,35.1249,35.0997,35.1458,35.1283,35.1165,35.0652,35.0462,35.0373,35.017,34.9793,34.9851,34.8776], 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"CCTTG":1491134, "CCTCA":1385754, "CCTCT":1661123, "CCTCC":1307317, "CCTCG":460100, "CCTGA":1504323, "CCTGT":2201798, "CCTGC":1282890, "CCTGG":1804162, "CCCAA":1086020, "CCCAT":1085853, "CCCAC":875585, "CCCAG":1298323, "CCCTA":686560, "CCCTT":855124, "CCCTC":924154, "CCCTG":1299815, "CCCCA":866879, "CCCCT":756793, "CCCCC":560159, "CCCCG":380944, "CCCGA":368080, "CCCGT":353082, "CCCGC":251719, "CCCGG":363415, "CCGAA":514764, "CCGAT":570848, "CCGAC":397280, "CCGAG":509292, "CCGTA":462313, "CCGTT":606881, "CCGTC":503381, "CCGTG":657489, "CCGCA":339803, "CCGCT":376351, "CCGCC":351698, "CCGCG":144112, "CCGGA":382261, "CCGGT":585341, "CCGGC":324211, "CCGGG":540858, "CGAAA":678049, "CGAAT":530631, "CGAAC":377625, "CGAAG":621500, "CGATA":681774, "CGATT":682624, "CGATC":521472, "CGATG":928555, "CGACA":721073, "CGACT":444780, "CGACC":318267, "CGACG":220138, "CGAGA":1225220, "CGAGT":607066, "CGAGC":466658, "CGAGG":887921, "CGTAA":635352, "CGTAT":542565, "CGTAC":425431, "CGTAG":678369, 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"GGGGC":1611254, "GGGGG":3791580 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 28669865, "total_bases": 2345166674, "q20_bases": 2265589466, "q30_bases": 2137068792, "total_cycles": 86, "quality_curves": { "A":[36.1724,36.1621,36.1846,36.2034,36.1346,36.1478,36.1077,36.1141,36.088,36.0848,36.0944,36.0527,36.0531,36.0595,36.0377,36.0338,36.0266,36.0071,35.9978,35.9811,35.9784,35.9545,35.9631,35.9242,35.9142,35.8979,35.8879,35.8716,35.8599,35.8314,35.8446,35.8192,35.7963,35.7818,35.7851,35.7554,35.7421,35.7006,35.6999,35.6465,35.6352,35.6295,35.6358,35.6045,35.5772,35.5364,35.524,35.5019,35.4936,35.4574,35.4358,35.3927,35.4043,35.3345,35.3756,35.3427,35.3581,35.3366,35.3319,35.2873,35.2673,35.2063,35.2073,35.1765,35.1799,35.1565,35.1855,35.1495,35.1086,35.1275,35.1065,35.1191,35.0944,35.0684,35.0409,35.0409,35.0026,34.9957,34.9768,34.9356,34.9104,34.9284,34.9364,34.8692,34.8597,34.5847], 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"GC":[0.432897,0.433661,0.426367,0.433082,0.427014,0.434658,0.431733,0.433701,0.425632,0.432895,0.428323,0.429333,0.429428,0.433271,0.424785,0.431165,0.42455,0.429755,0.427544,0.430527,0.424867,0.433371,0.426129,0.430604,0.427229,0.432309,0.424479,0.434095,0.427724,0.430903,0.428414,0.431854,0.426424,0.435715,0.427872,0.431221,0.428218,0.43279,0.427392,0.434646,0.428544,0.43227,0.427826,0.434135,0.426349,0.434955,0.428639,0.431811,0.430937,0.435844,0.428586,0.434887,0.426533,0.430987,0.431171,0.434201,0.428818,0.434649,0.425862,0.428922,0.429056,0.430266,0.420981,0.42984,0.422254,0.429098,0.42533,0.429382,0.420976,0.426537,0.422844,0.427005,0.42571,0.427267,0.422188,0.431389,0.425256,0.427316,0.42714,0.432781,0.429602,0.437461,0.428503,0.434052,0.434231,0.437733] }, "kmer_count": { "AAAAA":5903298, "AAAAT":4258782, "AAAAC":3337062, "AAAAG":2449231, "AAATA":4441719, "AAATT":2594313, "AAATC":2453094, "AAATG":3727307, "AAACA":5045385, "AAACT":2799448, "AAACC":2540154, "AAACG":954458, 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"AGGCA":1512317, "AGGCT":1797662, "AGGCC":1416778, "AGGCG":488733, "AGGGA":2333830, "AGGGT":1024635, "AGGGC":887380, "AGGGG":794720, "TAAAA":3830861, "TAAAT":3404771, "TAAAC":2786806, "TAAAG":2078950, "TAATA":3099519, "TAATT":2898126, "TAATC":1846463, "TAATG":2667198, "TAACA":3686809, "TAACT":2623287, "TAACC":3337415, "TAACG":657195, "TAAGA":1439044, "TAAGT":1358614, "TAAGC":901949, "TAAGG":910032, "TATAA":2943176, "TATAT":5253312, "TATAC":2997018, "TATAG":2340287, "TATTA":3375307, "TATTT":5813043, "TATTC":3157242, "TATTG":2580944, "TATCA":3383611, "TATCT":4087426, "TATCC":2795963, "TATCG":687268, "TATGA":2198097, "TATGT":3090399, "TATGC":1481754, "TATGG":1467624, "TACAA":3064617, "TACAT":5344950, "TACAC":4357113, "TACAG":3504952, "TACTA":4042176, "TACTT":2576276, "TACTC":2803258, "TACTG":3810560, "TACCA":3288013, "TACCT":3412048, "TACCC":2539486, "TACCG":694976, "TACGA":607977, "TACGT":761681, "TACGC":494151, "TACGG":474333, "TAGAA":2110762, "TAGAT":2335950, "TAGAC":1897146, 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"CACAA":3811086, "CACAT":4669362, "CACAC":11679051, "CACAG":3912806, "CACTA":4098319, "CACTT":2953803, "CACTC":4664921, "CACTG":3928271, "CACCA":4769778, "CACCT":3577661, "CACCC":3156010, "CACCG":888918, "CACGA":649940, "CACGT":913461, "CACGC":1024049, "CACGG":656636, "CAGAA":2691830, "CAGAT":2910249, "CAGAC":3101375, "CAGAG":2707422, "CAGTA":2984796, "CAGTT":2597446, "CAGTC":3062110, "CAGTG":2521559, "CAGCA":3251969, "CAGCT":2742631, "CAGCC":2656048, "CAGCG":721348, "CAGGA":2083889, "CAGGT":1923835, "CAGGC":1645213, "CAGGG":1349866, "CTAAA":2498231, "CTAAT":2224909, "CTAAC":2986421, "CTAAG":902493, "CTATA":3549264, "CTATT":3186369, "CTATC":3686117, "CTATG":1971376, "CTACA":4508718, "CTACT":5084890, "CTACC":3923854, "CTACG":705508, "CTAGA":1657686, "CTAGT":1797487, "CTAGC":1634550, "CTAGG":1138187, "CTTAA":1864158, "CTTAT":2300579, "CTTAC":2042157, "CTTAG":1066512, "CTTTA":3348216, "CTTTT":4139273, "CTTTC":4830932, "CTTTG":2803196, "CTTCA":3675576, "CTTCT":5038480, "CTTCC":3697967, "CTTCG":635738, "CTTGA":1705640, "CTTGT":2215009, "CTTGC":1372741, "CTTGG":1091954, "CTCAA":2833612, "CTCAT":3502668, "CTCAC":4502027, "CTCAG":2592679, "CTCTA":5403639, "CTCTT":5958554, "CTCTC":23951672, "CTCTG":6252549, "CTCCA":4935860, "CTCCT":7206951, "CTCCC":5310838, "CTCCG":925728, "CTCGA":535431, "CTCGT":873469, "CTCGC":1170487, "CTCGG":818251, "CTGAA":2622460, "CTGAT":2474397, "CTGAC":3085758, "CTGAG":1958619, "CTGTA":4335339, "CTGTT":5001529, "CTGTC":6428025, "CTGTG":3248402, "CTGCA":2857523, "CTGCT":3558009, "CTGCC":2673677, "CTGCG":576016, "CTGGA":2042866, "CTGGT":2041550, "CTGGC":1683426, "CTGGG":1299043, "CCAAA":2939188, "CCAAT":2276763, "CCAAC":2845415, "CCAAG":1674230, "CCATA":2752023, "CCATT":3415433, "CCATC":4238063, "CCATG":2560955, "CCACA":4401649, "CCACT":4095117, "CCACC":3903392, "CCACG":899968, "CCAGA":2795194, "CCAGT":2625636, "CCAGC":2318032, "CCAGG":1844901, "CCTAA":2179236, "CCTAT":2452871, "CCTAC":3338505, "CCTAG":1404310, "CCTTA":2114243, "CCTTT":3626496, "CCTTC":3895265, "CCTTG":1754670, "CCTCA":3781312, "CCTCT":8473110, "CCTCC":5995864, "CCTCG":840232, "CCTGA":2294386, "CCTGT":3761081, "CCTGC":2365509, "CCTGG":1906654, "CCCAA":2401317, "CCCAT":2872586, "CCCAC":3283423, "CCCAG":2727904, "CCCTA":3205153, "CCCTT":2993115, "CCCTC":6003137, "CCCTG":2998601, "CCCCA":3438151, "CCCCT":4246057, "CCCCC":3316331, "CCCCG":910557, "CCCGA":508515, "CCCGT":767833, "CCCGC":664391, "CCCGG":531149, "CCGAA":445860, "CCGAT":596034, "CCGAC":617180, "CCGAG":556135, "CCGTA":662917, "CCGTT":779295, "CCGTC":929924, "CCGTG":615542, "CCGCA":581939, "CCGCT":771870, "CCGCC":810895, "CCGCG":194911, "CCGGA":450522, "CCGGT":536872, "CCGGC":502037, "CCGGG":440337, "CGAAA":562212, "CGAAT":469930, "CGAAC":463685, "CGAAG":373102, "CGATA":544974, "CGATT":578156, "CGATC":523828, "CGATG":593307, "CGACA":816986, "CGACT":601591, "CGACC":690012, "CGACG":278817, "CGAGA":718306, "CGAGT":409557, "CGAGC":411881, "CGAGG":469719, "CGTAA":522703, "CGTAT":601630, "CGTAC":557672, "CGTAG":544173, "CGTTA":761456, "CGTTT":1110631, "CGTTC":838446, "CGTTG":731441, "CGTCA":889097, "CGTCT":1108307, "CGTCC":758958, "CGTCG":1345365, "CGTGA":528799, "CGTGT":1877153, "CGTGC":534398, "CGTGG":564272, "CGCAA":568099, "CGCAT":539888, "CGCAC":742746, "CGCAG":613084, "CGCTA":661505, "CGCTT":608347, "CGCTC":1374274, "CGCTG":893564, "CGCCA":762106, "CGCCT":677953, "CGCCC":842504, "CGCCG":364006, "CGCGA":151774, "CGCGT":181019, "CGCGC":265651, "CGCGG":165328, "CGGAA":1705829, "CGGAT":396141, "CGGAC":483231, "CGGAG":574848, "CGGTA":521450, "CGGTT":492031, "CGGTC":526808, "CGGTG":1578877, "CGGCA":537751, "CGGCT":569637, "CGGCC":558342, "CGGCG":249374, "CGGGA":424021, "CGGGT":321465, "CGGGC":329952, "CGGGG":385604, "GAAAA":2631803, "GAAAT":2438414, "GAAAC":2071075, "GAAAG":2670448, "GAATA":1857033, "GAATT":1673634, "GAATC":1219929, "GAATG":1797657, "GAACA":2476992, "GAACT":1513610, "GAACC":1420361, "GAACG":636353, "GAAGA":3010830, "GAAGT":1469726, "GAAGC":1128518, "GAAGG":1220593, "GATAA":1430192, "GATAT":1774992, "GATAC":1268459, "GATAG":970666, "GATTA":1530117, "GATTT":2315242, "GATTC":1316170, "GATTG":1417469, "GATCA":1583356, "GATCT":1998502, "GATCC":1105678, "GATCG":1724367, "GATGA":1935200, "GATGT":2079848, "GATGC":1232822, "GATGG":1296587, "GACAA":2214121, "GACAT":2527595, "GACAC":2599372, "GACAG":2471199, "GACTA":1668635, "GACTT":1593747, "GACTC":1675151, "GACTG":2048614, "GACCA":2237506, "GACCT":1607636, "GACCC":1634951, "GACCG":462452, "GACGA":681039, "GACGT":607540, "GACGC":470194, "GACGG":500031, "GAGAA":2076266, "GAGAT":1787703, "GAGAC":1787697, "GAGAG":2552222, "GAGTA":1144709, "GAGTT":1322246, "GAGTC":1069119, "GAGTG":2398968, "GAGCA":1434879, "GAGCT":1399349, "GAGCC":1213251, "GAGCG":1598864, "GAGGA":1784298, "GAGGT":1195364, "GAGGC":1058063, "GAGGG":1032496, "GTAAA":2330290, "GTAAT":1962859, "GTAAC":1597468, "GTAAG":937354, "GTATA":2312163, "GTATT":2688918, "GTATC":1579697, "GTATG":1651789, "GTACA":2421847, "GTACT":1770374, "GTACC":1523790, "GTACG":434555, "GTAGA":2088435, "GTAGT":1480225, "GTAGC":1242120, "GTAGG":1966404, "GTTAA":1924511, "GTTAT":2214948, "GTTAC":1830712, "GTTAG":1261188, "GTTTA":3540085, "GTTTT":3825474, "GTTTC":2623629, "GTTTG":2452695, "GTTCA":2223168, "GTTCT":2981712, "GTTCC":1814839, "GTTCG":380110, "GTTGA":1893666, "GTTGT":2606725, "GTTGC":1309263, "GTTGG":1203380, "GTCAA":1919798, "GTCAT":2506974, "GTCAC":2178000, "GTCAG":1984411, "GTCTA":1910257, "GTCTT":2327619, "GTCTC":4127493, "GTCTG":3083293, "GTCCA":2046622, "GTCCT":2441501, "GTCCC":1955903, "GTCCG":414079, "GTCGA":403739, "GTCGT":1648141, "GTCGC":544889, "GTCGG":399683, "GTGAA":1772969, "GTGAT":1559594, "GTGAC":1463875, "GTGAG":1179885, "GTGTA":3730685, "GTGTT":4122086, "GTGTC":2071267, "GTGTG":4063098, "GTGCA":1603638, "GTGCT":1570913, "GTGCC":1191374, "GTGCG":439082, "GTGGA":1563235, "GTGGT":1683147, "GTGGC":1108358, "GTGGG":919203, "GCAAA":1725690, "GCAAT":1431004, "GCAAC":1456357, "GCAAG":1090699, "GCATA":1352722, "GCATT":2000054, "GCATC":1575952, "GCATG":1493137, "GCACA":2292302, "GCACT":1618943, "GCACC":1488061, "GCACG":485614, "GCAGA":1803717, "GCAGT":1614607, "GCAGC":1851108, "GCAGG":1335954, "GCTAA":1361537, "GCTAT":1616373, "GCTAC":1818779, "GCTAG":1106565, "GCTTA":919492, "GCTTT":2329582, "GCTTC":1684000, "GCTTG":1058626, "GCTCA":1683793, "GCTCT":2969780, "GCTCC":1727792, "GCTCG":458106, "GCTGA":1704040, "GCTGT":2607618, "GCTGC":1907514, "GCTGG":1346632, "GCCAA":1703728, "GCCAT":2043216, "GCCAC":1841544, "GCCAG":1499112, "GCCTA":1342775, "GCCTT":1766320, "GCCTC":2002502, "GCCTG":1509940, "GCCCA":1668939, "GCCCT":1781815, "GCCCC":1565288, "GCCCG":403829, "GCCGA":453371, "GCCGT":551681, "GCCGC":488182, "GCCGG":416674, "GCGAA":359783, "GCGAT":343167, "GCGAC":370647, "GCGAG":350967, "GCGTA":313738, "GCGTT":545960, "GCGTC":1555009, "GCGTG":484906, "GCGCA":439913, "GCGCT":541323, "GCGCC":415387, "GCGCG":190248, "GCGGA":292951, "GCGGT":1192343, "GCGGC":353504, "GCGGG":281604, "GGAAA":2997441, "GGAAT":1384524, "GGAAC":1248036, "GGAAG":2407177, "GGATA":1000855, "GGATT":1300835, "GGATC":983679, "GGATG":1237663, "GGACA":1932099, "GGACT":1274122, "GGACC":1087379, "GGACG":453753, "GGAGA":1825393, "GGAGT":1126315, "GGAGC":1059354, "GGAGG":1410394, "GGTAA":1163884, "GGTAT":1212841, "GGTAC":1042887, "GGTAG":892916, "GGTTA":1176267, "GGTTT":1696917, "GGTTC":984046, "GGTTG":1115183, "GGTCA":1563836, "GGTCT":1428431, "GGTCC":985424, "GGTCG":470820, "GGTGA":1197952, "GGTGT":2365329, "GGTGC":865782, "GGTGG":1297360, "GGCAA":1095267, "GGCAT":1183114, "GGCAC":1054389, "GGCAG":1316582, "GGCTA":1294023, "GGCTT":1181618, "GGCTC":1187225, "GGCTG":1488618, "GGCCA":1525908, "GGCCT":1367376, "GGCCC":1014953, "GGCCG":409865, "GGCGA":364953, "GGCGT":347114, "GGCGC":360258, "GGCGG":1241945, "GGGAA":2193945, "GGGAT":919924, "GGGAC":864411, "GGGAG":1009809, "GGGTA":822133, "GGGTT":932069, "GGGTC":715700, "GGGTG":888598, "GGGCA":806966, "GGGCT":799305, "GGGCC":803625, "GGGCG":278389, "GGGGA":823165, "GGGGT":723908, "GGGGC":557073, "GGGGG":888687 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213499_sample_1703754_S133_L002_R1_001.fastq.gz --in2 lib_213499_sample_1703754_S133_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213499_sample_1703754_S133_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213499_sample_1703754_S133_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213499_sample_1703754_S133_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213499_sample_1703754_S133_L002_R2_001.fastp-trim.fq.gz " }