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"GC":[0.679158,0.503939,0.42331,0.427366,0.387348,0.368861,0.309405,0.388047,0.436051,0.339424,0.46795,0.460826,0.403722,0.397023,0.396752,0.397628,0.409651,0.404027,0.406849,0.405021,0.409985,0.403661,0.408385,0.403363,0.408434,0.405088,0.407298,0.403183,0.411907,0.40315,0.408357,0.403083,0.407654,0.404497,0.410108,0.406716,0.411161,0.409498,0.409166,0.408044,0.414587,0.405316,0.410586,0.409772,0.408362,0.410798,0.412815,0.406952,0.413759,0.408727,0.412022,0.410738,0.416834,0.412908,0.417779,0.412174,0.410769,0.411575,0.414023,0.407736,0.414853,0.411532,0.413891,0.413874,0.416053,0.409879,0.415766,0.415333,0.412908,0.414919,0.417493,0.412228,0.418004,0.415211,0.416715,0.416246,0.420448,0.416679,0.420732,0.418154,0.419174,0.419015,0.422082,0.416047,0.420943,0.421277,0.421621,0.420305,0.425577,0.420057,0.423797,0.422755,0.422996,0.424024,0.427672,0.42389,0.428501,0.427248,0.426931,0.42887,0.432211] }, "kmer_count": { "AAAAA":52656052, "AAAAT":22690979, "AAAAC":16870624, 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"GGTGT":7430332, "GGTGC":4381898, "GGTGG":9111476, "GGCAA":6390401, "GGCAT":4408895, "GGCAC":3854134, "GGCAG":6842226, "GGCTA":2477722, "GGCTT":4772453, "GGCTC":3656721, "GGCTG":7018639, "GGCCA":3960090, "GGCCT":3387547, "GGCCC":2224765, "GGCCG":2234561, "GGCGA":2930703, "GGCGT":2808401, "GGCGC":1695314, "GGCGG":2985590, "GGGAA":8737359, "GGGAT":6563324, "GGGAC":4312815, "GGGAG":10391208, "GGGTA":3816211, "GGGTT":6293804, "GGGTC":3781817, "GGGTG":7156327, "GGGCA":4949356, "GGGCT":4466705, "GGGCC":2698230, "GGGCG":2922259, "GGGGA":10016850, "GGGGT":6317182, "GGGGC":4599616, "GGGGG":16518796 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 66455074, "total_bases": 6711962474, "q20_bases": 6471420182, "q30_bases": 6126609959, "total_cycles": 101, "quality_curves": { 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"CTATC":4627117, "CTATG":4110031, "CTACA":8935798, "CTACT":8088873, "CTACC":4936316, "CTACG":2347568, "CTAGA":3958978, "CTAGT":2888948, "CTAGC":2483384, "CTAGG":1472274, "CTTAA":7831468, "CTTAT":6744609, "CTTAC":5040837, "CTTAG":2970748, "CTTTA":11041339, "CTTTT":19297841, "CTTTC":10615069, "CTTTG":10416800, "CTTCA":12464679, "CTTCT":15365567, "CTTCC":9458958, "CTTCG":2712954, "CTTGA":5629249, "CTTGT":7677689, "CTTGC":4766322, "CTTGG":3657831, "CTCAA":8221809, "CTCAT":9286233, "CTCAC":10976935, "CTCAG":8226179, "CTCTA":6310459, "CTCTT":12499005, "CTCTC":15989197, "CTCTG":12389095, "CTCCA":10840409, "CTCCT":14664139, "CTCCC":8061618, "CTCCG":3309377, "CTCGA":1929904, "CTCGT":4632804, "CTCGC":3062203, "CTCGG":2788957, "CTGAA":9970293, "CTGAT":8205218, "CTGAC":7118561, "CTGAG":6306694, "CTGTA":8683713, "CTGTT":12493111, "CTGTC":10555328, "CTGTG":9154507, "CTGCA":10253273, "CTGCT":11290204, "CTGCC":6352680, "CTGCG":2376605, "CTGGA":6725212, "CTGGT":5892679, "CTGGC":4682347, "CTGGG":3725750, "CCAAA":10135453, "CCAAT":4964205, "CCAAC":7863224, "CCAAG":5139610, "CCATA":4696894, "CCATT":8151253, "CCATC":8597015, "CCATG":5877383, "CCACA":9563532, "CCACT":7495821, "CCACC":8757903, "CCACG":3269193, "CCAGA":10139774, "CCAGT":6557637, "CCAGC":7228683, "CCAGG":4765398, "CCTAA":5104819, "CCTAT":3179685, "CCTAC":4825988, "CCTAG":1949215, "CCTTA":5359174, "CCTTT":10786575, "CCTTC":9805369, "CCTTG":4995106, "CCTCA":10880429, "CCTCT":13289219, "CCTCC":12951795, "CCTCG":3076410, "CCTGA":7131840, "CCTGT":7540871, "CCTGC":6554118, "CCTGG":4963300, "CCCAA":6306714, "CCCAT":5288252, "CCCAC":6529379, "CCCAG":6780726, "CCCTA":3963920, "CCCTT":6709322, "CCCTC":9722447, "CCCTG":5639745, "CCCCA":7481103, "CCCCT":7391363, "CCCCC":10239534, "CCCCG":3041229, "CCCGA":2126184, "CCCGT":2159278, "CCCGC":2273812, "CCCGG":2104675, "CCGAA":1624718, "CCGAT":1740199, "CCGAC":2562427, "CCGAG":2315021, "CCGTA":1621027, "CCGTT":2521172, "CCGTC":3550299, "CCGTG":2280055, "CCGCA":2325030, "CCGCT":2829131, "CCGCC":3026566, "CCGCG":1273170, "CCGGA":1840888, "CCGGT":1614496, "CCGGC":2334708, "CCGGG":1571349, "CGAAA":2165737, "CGAAT":1209957, "CGAAC":1381136, "CGAAG":1486121, "CGATA":1539368, "CGATT":1687130, "CGATC":1486666, "CGATG":2283568, "CGACA":3567519, "CGACT":2420259, "CGACC":3075063, "CGACG":1527816, "CGAGA":2717048, "CGAGT":1667800, "CGAGC":2107546, "CGAGG":1797488, "CGTAA":1866369, "CGTAT":1878406, "CGTAC":1927145, "CGTAG":1410200, "CGTTA":2247251, "CGTTT":3862960, "CGTTC":2378148, "CGTTG":2154726, "CGTCA":3332630, "CGTCT":4028828, "CGTCC":2940989, "CGTCG":9232560, "CGTGA":1961443, "CGTGT":9307065, "CGTGC":1725930, "CGTGG":3931040, "CGCAA":1944028, "CGCAT":2009742, "CGCAC":2311209, "CGCAG":2585175, "CGCTA":1659059, "CGCTT":2733985, "CGCTC":2936179, "CGCTG":3506250, "CGCCA":3039299, "CGCCT":2732428, "CGCCC":3043627, "CGCCG":2069730, "CGCGA":653726, "CGCGT":927286, "CGCGC":815651, "CGCGG":1482110, "CGGAA":11909572, "CGGAT":1481513, "CGGAC":1974875, "CGGAG":2661315, "CGGTA":1376737, "CGGTT":1892541, "CGGTC":1508529, "CGGTG":2735097, "CGGCA":2276328, "CGGCT":2460563, "CGGCC":2537032, "CGGCG":1574358, "CGGGA":1577135, "CGGGT":1260228, "CGGGC":1298643, "CGGGG":1995523, "GAAAA":11402359, "GAAAT":7945285, "GAAAC":6859012, "GAAAG":9964735, "GAATA":5401426, "GAATT":5549691, "GAATC":3754149, "GAATG":5054454, "GAACA":7182753, "GAACT":4458418, "GAACC":4153136, "GAACG":1952156, "GAAGA":16501342, "GAAGT":5258947, "GAAGC":4633047, "GAAGG":3865900, "GATAA":5240517, "GATAT":5539983, "GATAC":3234985, "GATAG":2225131, "GATTA":4762065, "GATTT":8489011, "GATTC":3993938, "GATTG":4171368, "GATCA":6153152, "GATCT":5149099, "GATCC":3722749, "GATCG":13274113, "GATGA":6967996, "GATGT":6586841, "GATGC":4372317, "GATGG":3871840, "GACAA":7664324, "GACAT":7476831, "GACAC":6245944, "GACAG":6273717, "GACTA":3428508, "GACTT":6078285, "GACTC":4821689, "GACTG":5486898, "GACCA":6109639, "GACCT":4523584, "GACCC":3620468, "GACCG":1793346, "GACGA":2606049, "GACGT":2351860, "GACGC":2247234, "GACGG":2201410, "GAGAA":6594524, "GAGAT":7286217, "GAGAC":4860453, "GAGAG":5442816, "GAGTA":3131541, "GAGTT":4935098, "GAGTC":3605437, "GAGTG":7370172, "GAGCA":5273168, "GAGCT":5095548, "GAGCC":3778910, "GAGCG":10499043, "GAGGA":6097256, "GAGGT":3342272, "GAGGC":3625032, "GAGGG":3006281, "GTAAA":7876667, "GTAAT":5866937, "GTAAC":4244422, "GTAAG":2866992, "GTATA":4771393, "GTATT":7467940, "GTATC":4208218, "GTATG":4143728, "GTACA":6468449, "GTACT":5044172, "GTACC":2565135, "GTACG":1427348, "GTAGA":5003086, "GTAGT":3801204, "GTAGC":2782899, "GTAGG":7365632, "GTTAA":6800132, "GTTAT":6129423, "GTTAC":4190995, "GTTAG":2912707, "GTTTA":9220819, "GTTTT":16430752, "GTTTC":8286148, "GTTTG":9910107, "GTTCA":7280828, "GTTCT":6667238, "GTTCC":4763645, "GTTCG":1477438, "GTTGA":5557527, "GTTGT":8118276, "GTTGC":4474448, "GTTGG":3923619, "GTCAA":8949219, "GTCAT":6993942, "GTCAC":5615342, "GTCAG":5656958, "GTCTA":3442512, "GTCTT":7196649, "GTCTC":6797937, "GTCTG":7258382, "GTCCA":6196859, "GTCCT":6724398, "GTCCC":4016297, "GTCCG":1810340, "GTCGA":1611481, "GTCGT":9177241, "GTCGC":2513769, "GTCGG":1931060, "GTGAA":6106370, "GTGAT":5311775, "GTGAC":4797364, "GTGAG":3884782, "GTGTA":12666189, "GTGTT":9361262, "GTGTC":8849324, "GTGTG":11281325, "GTGCA":5464255, "GTGCT":5427924, "GTGCC":3738008, "GTGCG":1473586, "GTGGA":4928477, "GTGGT":6376077, "GTGGC":3436261, "GTGGG":3165308, "GCAAA":7080630, "GCAAT":4184424, "GCAAC":5501187, "GCAAG":3994337, "GCATA":3694966, "GCATT":6505403, "GCATC":5251434, "GCATG":4674660, "GCACA":6845838, "GCACT":5561966, "GCACC":4304692, "GCACG":1607251, "GCAGA":7760230, "GCAGT":5436172, "GCAGC":8162819, "GCAGG":4458006, "GCTAA":4218429, "GCTAT":3618891, "GCTAC":3795390, "GCTAG":1988771, "GCTTA":3463342, "GCTTT":9258322, "GCTTC":6745819, "GCTTG":3455253, "GCTCA":6227418, "GCTCT":6672829, "GCTCC":5904310, "GCTCG":3920241, "GCTGA":6549141, "GCTGT":8283119, "GCTGC":7532291, "GCTGG":4975147, "GCCAA":5609996, "GCCAT":5752781, 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"GGTGA":3782863, "GGTGT":5882960, "GGTGC":3532712, "GGTGG":4147769, "GGCAA":3768718, "GGCAT":3233416, "GGCAC":2896354, "GGCAG":3972588, "GGCTA":2336343, "GGCTT":4325177, "GGCTC":4043608, "GGCTG":4680985, "GGCCA":4016408, "GGCCT":3815170, "GGCCC":2752726, "GGCCG":2259880, "GGCGA":1368480, "GGCGT":1629640, "GGCGC":1328882, "GGCGG":1809231, "GGGAA":8233903, "GGGAT":2432408, "GGGAC":2593959, "GGGAG":2859943, "GGGTA":1758668, "GGGTT":2695833, "GGGTC":2100953, "GGGTG":2835737, "GGGCA":2378822, "GGGCT":2743333, "GGGCC":2510347, "GGGCG":1489730, "GGGGA":2220799, "GGGGT":2200175, "GGGGC":2304800, "GGGGG":17106531 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 65148015, "total_bases": 5289232021, "q20_bases": 5151337155, "q30_bases": 4911520665, "total_cycles": 86, "quality_curves": { "A":[36.4519,36.4332,36.4307,36.4187,36.4155,36.4124,36.3981,36.388,36.3792,36.3668,36.3231,36.3118,36.3139,36.3122,36.3027,36.2855,36.286,36.2839,36.2778,36.2693,36.2517,36.2468,36.2445,36.2266,36.2226,36.2303,36.2201,36.2023,36.1875,36.1838,36.1885,36.1979,36.1889,36.1465,36.1634,36.1212,36.1181,36.1272,36.1186,36.1333,36.0806,36.0881,36.0933,36.0612,36.0694,36.0488,36.0271,36.0131,36.0326,35.9934,35.9898,35.9843,35.9636,35.9391,35.915,35.9291,35.909,35.9192,35.8945,35.8941,35.8574,35.8473,35.8516,35.8575,35.8426,35.8322,35.8045,35.817,35.8018,35.7676,35.7319,35.7379,35.7169,35.7292,35.706,35.7101,35.7261,35.7295,35.7122,35.6866,35.6487,35.6307,35.5964,35.6054,35.5968,35.502], "T":[36.0281,36.0142,36.0054,35.987,35.9908,35.9778,35.972,35.9343,35.9073,35.9015,35.824,35.7871,35.8084,35.8098,35.7957,35.7791,35.7813,35.7624,35.7528,35.7596,35.7156,35.7186,35.7089,35.6926,35.7089,35.6974,35.6938,35.675,35.6519,35.651,35.6361,35.6182,35.6178,35.6234,35.595,35.5942,35.5932,35.5429,35.5669,35.5317,35.5906,35.5108,35.5522,35.5284,35.5062,35.5734,35.5148,35.4763,35.5288,35.4644,35.4136,35.4005,35.4336,35.4082,35.4339,35.4566,35.4588,35.4543,35.4292,35.3929,35.3596,35.3674,35.3514,35.3452,35.3443,35.3033,35.2706,35.3167,35.3005,35.3016,35.2641,35.2342,35.2366,35.259,35.1881,35.2296,35.2465,35.2526,35.2241,35.2031,35.1757,35.1758,35.1451,35.1222,35.1031,35.0949], "C":[36.3822,36.4016,36.3758,36.3659,36.35,36.3459,36.3251,36.3222,36.2985,36.2849,36.2392,36.2355,36.2403,36.2245,36.2075,36.1942,36.1856,36.1821,36.1822,36.1608,36.1574,36.1621,36.1497,36.1318,36.1104,36.1012,36.0818,36.085,36.0687,36.0638,36.0795,36.0611,36.033,36.0397,36.0405,36.0391,35.9964,36.0074,35.9851,35.9955,35.9521,35.9628,35.9618,35.947,35.9352,35.9357,35.9004,35.9038,35.871,35.878,35.8235,35.8495,35.8389,35.848,35.8537,35.8435,35.8256,35.8172,35.7801,35.757,35.7483,35.7259,35.7125,35.7273,35.7206,35.6931,35.6764,35.6762,35.6704,35.6466,35.6261,35.6146,35.608,35.6138,35.5759,35.5805,35.5847,35.5679,35.5596,35.5421,35.5208,35.5071,35.5117,35.4631,35.4985,35.3367], "G":[36.0221,36.0401,36.0269,36.0209,36.0075,36.0158,35.9887,35.9872,35.9667,35.9642,35.874,35.8712,35.8732,35.8616,35.8314,35.825,35.8353,35.8233,35.8043,35.8034,35.7697,35.7642,35.7506,35.7383,35.7418,35.7384,35.7032,35.674,35.6681,35.6649,35.682,35.5929,35.6144,35.6457,35.5934,35.609,35.5916,35.6195,35.5571,35.5273,35.5579,35.5172,35.5358,35.5378,35.447,35.5131,35.4942,35.5038,35.4413,35.4629,35.3846,35.4686,35.4382,35.404,35.391,35.418,35.4407,35.4247,35.4047,35.3593,35.3458,35.3302,35.3533,35.3364,35.3165,35.2901,35.2449,35.2812,35.2622,35.2041,35.1648,35.1438,35.1657,35.1671,35.1286,35.1017,35.1559,35.131,35.1233,35.0954,35.0612,35.0321,35.0408,34.9952,35.0029,34.853], 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"CTAGG":1441665, "CTTAA":5137272, "CTTAT":3559342, "CTTAC":2249625, "CTTAG":2556090, "CTTTA":6638577, "CTTTT":8922859, "CTTTC":4171131, "CTTTG":7154654, "CTTCA":6100551, "CTTCT":5600502, "CTTCC":3093028, "CTTCG":1096148, "CTTGA":4986537, "CTTGT":5406794, "CTTGC":2993690, "CTTGG":3937629, "CTCAA":4543899, "CTCAT":4620600, "CTCAC":2983519, "CTCAG":4626596, "CTCTA":2971482, "CTCTT":4815941, "CTCTC":3984789, "CTCTG":6340508, "CTCCA":4576492, "CTCCT":4726626, "CTCCC":2045042, "CTCCG":1861488, "CTCGA":1256021, "CTCGT":1766227, "CTCGC":1178961, "CTCGG":1406215, "CTGAA":7993982, "CTGAT":6273218, "CTGAC":4460673, "CTGAG":6433434, "CTGTA":6192500, "CTGTT":8059269, "CTGTC":4789470, "CTGTG":8311224, "CTGCA":7181331, "CTGCT":7670287, "CTGCC":2927427, "CTGCG":1791788, "CTGGA":6308484, "CTGGT":5858453, "CTGGC":3484587, "CTGGG":4538610, "CCAAA":6061947, "CCAAT":3183647, "CCAAC":3097363, "CCAAG":2960691, "CCATA":2950268, "CCATT":3813522, "CCATC":3082495, "CCATG":3882446, "CCACA":4761625, "CCACT":2903720, "CCACC":2257291, "CCACG":1535952, "CCAGA":4787918, "CCAGT":3849909, "CCAGC":3910565, "CCAGG":3743698, "CCTAA":2002240, "CCTAT":1504754, "CCTAC":1430373, "CCTAG":1220612, "CCTTA":2148874, "CCTTT":4802198, "CCTTC":3043626, "CCTTG":3459274, "CCTCA":3558098, "CCTCT":3954638, "CCTCC":3429880, "CCTCG":1384496, "CCTGA":4145345, "CCTGT":4557383, "CCTGC":3487138, "CCTGG":3862702, "CCCAA":2485009, "CCCAT":2248906, "CCCAC":1967110, "CCCAG":2938781, "CCCTA":1195285, "CCCTT":2041116, "CCCTC":2135790, "CCCTG":2843562, "CCCCA":1491747, "CCCCT":1687416, "CCCCC":1141838, "CCCCG":993929, "CCCGA":1024877, "CCCGT":1086323, "CCCGC":881591, "CCCGG":1038537, "CCGAA":1315301, "CCGAT":1517736, "CCGAC":1455300, "CCGAG":1417575, "CCGTA":1200367, "CCGTT":1700545, "CCGTC":1609960, "CCGTG":1706386, "CCGCA":921080, "CCGCT":1120809, "CCGCC":1239721, "CCGCG":953190, "CCGGA":1351922, "CCGGT":1343554, "CCGGC":1169483, "CCGGG":1629552, "CGAAA":1801183, "CGAAT":1375919, "CGAAC":1101002, "CGAAG":2171477, "CGATA":1422414, "CGATT":1466079, "CGATC":1398017, "CGATG":2607960, "CGACA":2240418, "CGACT":1253334, "CGACC":1145170, "CGACG":1384878, "CGAGA":1869664, "CGAGT":1705417, "CGAGC":1538553, "CGAGG":2482976, "CGTAA":1382687, "CGTAT":1286693, "CGTAC":1135064, "CGTAG":1792155, "CGTTA":1738195, "CGTTT":2846635, "CGTTC":1492605, "CGTTG":2340449, "CGTCA":2146208, "CGTCT":2417026, "CGTCC":1524383, "CGTCG":1055750, "CGTGA":1853700, "CGTGT":2517800, "CGTGC":1212515, "CGTGG":2744180, "CGCAA":1250945, "CGCAT":992147, "CGCAC":1200016, "CGCAG":1876465, "CGCTA":796049, "CGCTT":1623881, "CGCTC":1364417, "CGCTG":2453829, "CGCCA":1154704, "CGCCT":1185130, "CGCCC":848120, "CGCCG":1254440, "CGCGA":666824, "CGCGT":521077, "CGCGC":635725, "CGCGG":834225, "CGGAA":1412757, "CGGAT":1524346, "CGGAC":1386497, "CGGAG":2476404, "CGGTA":1107405, "CGGTT":1910823, "CGGTC":1385924, "CGGTG":2666097, "CGGCA":1513329, "CGGCT":1646047, "CGGCC":1498476, "CGGCG":1154315, "CGGGA":1672911, "CGGGT":1395724, "CGGGC":1355617, 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"GAGCT":5510050, "GAGCC":3086687, "GAGCG":2400886, "GAGGA":14269383, "GAGGT":6373175, "GAGGC":4924565, "GAGGG":8629131, "GTAAA":8588048, "GTAAT":6034299, "GTAAC":3414497, "GTAAG":4205083, "GTATA":5029818, "GTATT":6210541, "GTATC":2608647, "GTATG":5171114, "GTACA":5773631, "GTACT":3828937, "GTACC":1669984, "GTACG":1441701, "GTAGA":5619143, "GTAGT":7143146, "GTAGC":3074257, "GTAGG":3768970, "GTTAA":6943191, "GTTAT":5214756, "GTTAC":3560997, "GTTAG":4254224, "GTTTA":7572048, "GTTTT":10355022, "GTTTC":5408360, "GTTTG":8907154, "GTTCA":5690334, "GTTCT":4585333, "GTTCC":2620678, "GTTCG":973623, "GTTGA":6465714, "GTTGT":9909960, "GTTGC":4333406, "GTTGG":6171009, "GTCAA":5302239, "GTCAT":5250572, "GTCAC":3906739, "GTCAG":5702368, "GTCTA":2652616, "GTCTT":4675047, "GTCTC":3727217, "GTCTG":6754495, "GTCCA":4269363, "GTCCT":3660830, "GTCCC":1924187, "GTCCG":1503990, "GTCGA":2164245, "GTCGT":2402977, "GTCGC":1398244, "GTCGG":1963125, "GTGAA":8404756, "GTGAT":6160390, "GTGAC":4590213, "GTGAG":8978490, "GTGTA":7714159, "GTGTT":9164491, "GTGTC":4866753, "GTGTG":21003316, "GTGCA":5746482, "GTGCT":4773815, "GTGCC":2254452, "GTGCG":1759261, "GTGGA":7647974, "GTGGT":6500012, "GTGGC":3794524, "GTGGG":5267845, "GCAAA":7771190, "GCAAT":4565146, "GCAAC":3687330, "GCAAG":3906409, "GCATA":3579969, "GCATT":5423297, "GCATC":3493741, "GCATG":4802235, "GCACA":5225207, "GCACT":3475405, "GCACC":2972776, "GCACG":1414564, "GCAGA":7257201, "GCAGT":5508844, "GCAGC":6030546, "GCAGG":5334994, "GCTAA":3389622, "GCTAT":2341732, "GCTAC":2247842, "GCTAG":2095542, "GCTTA":2878276, "GCTTT":6385604, "GCTTC":3634754, "GCTTG":3849807, "GCTCA":4172351, "GCTCT":3897434, "GCTCC":3072145, "GCTCG":1590703, "GCTGA":6255626, "GCTGT":6911810, "GCTGC":6466189, "GCTGG":5728880, "GCCAA":3550034, "GCCAT":3172184, "GCCAC":2582016, "GCCAG":3736410, "GCCTA":1266831, "GCCTT":2983853, "GCCTC":2868656, "GCCTG":3419511, "GCCCA":2455243, "GCCCT":1927611, "GCCCC":1432571, "GCCCG":1005673, "GCCGA":1631131, 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"GGCTG":5755278, "GGCCA":3258566, "GGCCT":2820891, "GGCCC":1851920, "GGCCG":1833322, "GGCGA":2225008, "GGCGT":2078996, "GGCGC":1317067, "GGCGG":2078717, "GGGAA":6042548, "GGGAT":4801536, "GGGAC":3308576, "GGGAG":7096391, "GGGTA":2914580, "GGGTT":4584068, "GGGTC":2939120, "GGGTG":5184485, "GGGCA":3726218, "GGGCT":3471982, "GGGCC":2222850, "GGGCG":2127488, "GGGGA":7033805, "GGGGT":4336135, "GGGGC":3442735, "GGGGG":7480090 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 65148015, "total_bases": 5390609041, "q20_bases": 5232069912, "q30_bases": 4957198280, "total_cycles": 86, "quality_curves": { "A":[36.1887,36.1733,36.1991,36.2187,36.1564,36.1712,36.1295,36.1416,36.1208,36.1231,36.1321,36.0915,36.0963,36.0981,36.0637,36.0643,36.0633,36.0521,36.0432,36.022,36.0233,35.9984,36.0107,35.9778,35.9777,35.9499,35.9628,35.9367,35.9243,35.9126,35.9289,35.9118,35.8964,35.8886,35.8883,35.8587,35.8496,35.8152,35.8144,35.7765,35.7765,35.7691,35.7869,35.7558,35.7306,35.7079,35.6898,35.681,35.6717,35.6358,35.624,35.6008,35.5997,35.5468,35.5738,35.5466,35.5633,35.5493,35.5433,35.5185,35.4968,35.4463,35.4537,35.4398,35.4541,35.4379,35.4367,35.4159,35.3871,35.4041,35.3756,35.3826,35.3597,35.3443,35.3307,35.3348,35.3011,35.2996,35.2946,35.2581,35.2532,35.2719,35.282,35.22,35.2094,34.9708], "T":[35.9169,35.8809,35.9054,35.9032,35.8567,35.8583,35.8024,35.8015,35.777,35.7803,35.7986,35.7668,35.7468,35.732,35.7117,35.6962,35.7158,35.6856,35.6878,35.6752,35.6559,35.6672,35.6584,35.6218,35.6226,35.5881,35.6079,35.5961,35.5826,35.5509,35.5584,35.5249,35.5303,35.5197,35.5042,35.4815,35.4887,35.4592,35.4713,35.4419,35.4333,35.4173,35.4149,35.3943,35.3741,35.3661,35.373,35.3806,35.3392,35.311,35.3077,35.3038,35.3175,35.2599,35.2508,35.2487,35.2429,35.2254,35.2114,35.1773,35.1655,35.1306,35.1402,35.1544,35.1542,35.2204,35.1695,35.1913,35.1306,35.143,35.1081,35.0855,35.0712,35.0364,35.0204,35.067,35.0274,35.0461,35.0112,34.9759,34.9121,34.9377,34.9245,34.9235,34.9324,34.7009], "C":[36.3432,36.345,36.3364,36.3294,36.2956,36.299,36.2797,36.2697,36.2422,36.2452,36.2427,36.2175,36.1931,36.2019,36.1693,36.1772,36.1554,36.1348,36.1384,36.1064,36.1193,36.1077,36.1047,36.0905,36.079,36.0604,36.0677,36.0379,36.0321,36.0017,36.0024,36.0086,35.9754,35.975,35.9625,35.956,35.9382,35.9261,35.9147,35.8756,35.8839,35.8595,35.869,35.8654,35.8263,35.8294,35.8074,35.7914,35.7922,35.7406,35.7112,35.7122,35.7336,35.6794,35.6755,35.6402,35.6214,35.6159,35.638,35.6387,35.6234,35.5809,35.571,35.5877,35.5446,35.5415,35.4781,35.4745,35.4332,35.4504,35.3949,35.401,35.3798,35.3344,35.319,35.3608,35.3313,35.3049,35.3203,35.2716,35.2547,35.2663,35.2751,35.234,35.2155,34.9479], "G":[36.159,36.148,36.1538,36.1476,36.0849,36.0822,36.0553,36.0473,36.02,36.0184,36.0196,36.0001,35.9785,35.9788,35.9511,35.9286,35.9068,35.8971,35.9096,35.8999,35.8922,35.8781,35.8716,35.8564,35.8403,35.8351,35.8082,35.8168,35.8101,35.8008,35.777,35.7822,35.7303,35.7164,35.7128,35.6988,35.675,35.6211,35.6403,35.5823,35.6191,35.5929,35.5935,35.5771,35.5635,35.5117,35.5188,35.5034,35.4973,35.4458,35.4479,35.4021,35.4146,35.3433,35.355,35.3039,35.3415,35.2902,35.2997,35.238,35.2132,35.1869,35.1582,35.1234,35.1541,35.0903,35.1363,35.0958,35.076,35.091,35.0402,34.9984,34.9758,34.9043,34.8894,34.8527,34.9418,34.8187,34.9023,34.7714,34.8069,34.7737,34.8543,34.7747,34.7453,34.4632], 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"CTTAA":6367407, "CTTAT":5596906, "CTTAC":4102328, "CTTAG":2317285, "CTTTA":8955030, "CTTTT":15724549, "CTTTC":8543554, "CTTTG":8365087, "CTTCA":9757689, "CTTCT":12520585, "CTTCC":7581905, "CTTCG":2160951, "CTTGA":4387034, "CTTGT":6153122, "CTTGC":3840241, "CTTGG":2848249, "CTCAA":6471418, "CTCAT":7455480, "CTCAC":8757897, "CTCAG":6310656, "CTCTA":4995216, "CTCTT":10192010, "CTCTC":12924383, "CTCTG":9950515, "CTCCA":8339335, "CTCCT":11877693, "CTCCC":6477183, "CTCCG":2521188, "CTCGA":1488065, "CTCGT":3916076, "CTCGC":2423605, "CTCGG":2194828, "CTGAA":7966727, "CTGAT":6672446, "CTGAC":5660954, "CTGAG":4846913, "CTGTA":7021055, "CTGTT":10206703, "CTGTC":8436873, "CTGTG":7362680, "CTGCA":8198883, "CTGCT":9067877, "CTGCC":5106649, "CTGCG":1896835, "CTGGA":5317832, "CTGGT":4669116, "CTGGC":3731284, "CTGGG":2989127, "CCAAA":8198024, "CCAAT":4090963, "CCAAC":6125047, "CCAAG":3993478, "CCATA":3812264, "CCATT":6757914, "CCATC":6942232, "CCATG":4770281, "CCACA":7332559, "CCACT":6053809, "CCACC":6812857, "CCACG":2596458, "CCAGA":6036164, "CCAGT":5255274, "CCAGC":5797459, "CCAGG":3853891, "CCTAA":4133537, "CCTAT":2628353, "CCTAC":3873550, "CCTAG":1443199, "CCTTA":4329309, "CCTTT":8877111, "CCTTC":8028350, "CCTTG":4072247, "CCTCA":8787094, "CCTCT":10823269, "CCTCC":10486180, "CCTCG":2446449, "CCTGA":5660356, "CCTGT":5991822, "CCTGC":5259946, "CCTGG":3954968, "CCCAA":4786704, "CCCAT":4259381, "CCCAC":4909706, "CCCAG":4656855, "CCCTA":3291380, "CCCTT":5533809, "CCCTC":7936626, "CCCTG":4573677, "CCCCA":5371913, "CCCCT":6142011, "CCCCC":6535406, "CCCCG":2172134, "CCCGA":1550454, "CCCGT":1691446, "CCCGC":1656341, "CCCGG":1538415, "CCGAA":1298498, "CCGAT":1361761, "CCGAC":1993339, "CCGAG":1641568, "CCGTA":1252056, "CCGTT":2004529, "CCGTC":2834183, "CCGTG":1740343, "CCGCA":1769362, "CCGCT":2246963, "CCGCC":2259923, "CCGCG":902386, "CCGGA":1406745, "CCGGT":1272968, "CCGGC":1722854, "CCGGG":1110013, "CGAAA":1767771, "CGAAT":974274, "CGAAC":1077659, "CGAAG":1091325, "CGATA":1244173, "CGATT":1359623, "CGATC":1154126, "CGATG":1841872, "CGACA":2717633, "CGACT":1920757, "CGACC":2383951, "CGACG":1166195, "CGAGA":1726954, "CGAGT":1308375, "CGAGC":1673527, "CGAGG":1421281, "CGTAA":1522372, "CGTAT":1503721, "CGTAC":1590569, "CGTAG":1129479, "CGTTA":1780780, "CGTTT":3090446, "CGTTC":1929657, "CGTTG":1709141, "CGTCA":2655510, "CGTCT":3179579, "CGTCC":2342017, "CGTCG":3338987, "CGTGA":1554048, "CGTGT":4251487, "CGTGC":1389616, "CGTGG":3188154, "CGCAA":1544099, "CGCAT":1619942, "CGCAC":1845008, "CGCAG":1942648, "CGCTA":1311619, "CGCTT":2092333, "CGCTC":2360525, "CGCTG":2805563, "CGCCA":2281744, "CGCCT":2181298, "CGCCC":2159520, "CGCCG":1523089, "CGCGA":516154, "CGCGT":704386, "CGCGC":526571, "CGCGG":970744, "CGGAA":3238990, "CGGAT":1148876, "CGGAC":1530115, "CGGAG":2010141, "CGGTA":1102326, "CGGTT":1523865, "CGGTC":1186648, "CGGTG":2193693, "CGGCA":1733053, "CGGCT":1867452, "CGGCC":1875407, "CGGCG":1165913, "CGGGA":1231497, "CGGGT":924177, "CGGGC":940919, "CGGGG":1149151, "GAAAA":9555407, "GAAAT":6735834, "GAAAC":5713273, "GAAAG":6487734, "GAATA":4519679, "GAATT":4677708, "GAATC":3115564, "GAATG":4216588, "GAACA":5823250, "GAACT":3658781, "GAACC":3401430, "GAACG":1569214, "GAAGA":7532720, "GAAGT":4320661, "GAAGC":3798477, "GAAGG":3092974, "GATAA":4415580, "GATAT":4673961, "GATAC":2682146, "GATAG":1779471, "GATTA":4003387, "GATTT":7147545, "GATTC":3304467, "GATTG":3475280, "GATCA":5014608, "GATCT":4252339, "GATCC":2997975, "GATCG":3431131, "GATGA":5811871, "GATGT":5524643, "GATGC":3561178, "GATGG":3201628, "GACAA":6263740, "GACAT":6168598, "GACAC":5049978, "GACAG":4900127, "GACTA":2790066, "GACTT":4959093, "GACTC":3901297, "GACTG":4453072, "GACCA":4896003, "GACCT":3649884, "GACCC":2915772, "GACCG":1435730, "GACGA":2079340, "GACGT":1902764, "GACGC":1809683, "GACGG":1752930, "GAGAA":5489675, "GAGAT":4464244, "GAGAC":3981709, "GAGAG":4326075, "GAGTA":2609871, "GAGTT":4114397, "GAGTC":2928529, "GAGTG":5018255, "GAGCA":4292954, "GAGCT":4125755, "GAGCC":3081179, "GAGCG":3529441, "GAGGA":5055964, "GAGGT":2716202, "GAGGC":2957319, "GAGGG":2291061, "GTAAA":6585791, "GTAAT":4964116, "GTAAC":3535743, "GTAAG":2323969, "GTATA":4004980, "GTATT":6369444, "GTATC":3447690, "GTATG":3452030, "GTACA":5340471, "GTACT":4222859, "GTACC":2095081, "GTACG":1185612, "GTAGA":4081550, "GTAGT":3169110, "GTAGC":2314750, "GTAGG":3429551, "GTTAA":5564436, "GTTAT":5127025, "GTTAC":3425970, "GTTAG":2311454, "GTTTA":7469146, "GTTTT":13326011, "GTTTC":6722835, "GTTTG":7918851, "GTTCA":5816746, "GTTCT":5398919, "GTTCC":3896485, "GTTCG":1160929, "GTTGA":4451221, "GTTGT":6547602, "GTTGC":3610233, "GTTGG":3057692, "GTCAA":6847707, "GTCAT":5758998, "GTCAC":4494738, "GTCAG":4491805, "GTCTA":2765894, "GTCTT":5873329, "GTCTC":5467582, "GTCTG":5782977, "GTCCA":4897296, "GTCCT":5432657, "GTCCC":3235146, "GTCCG":1447574, "GTCGA":1235923, "GTCGT":3624352, "GTCGC":1931250, "GTCGG":1425636, "GTGAA":4969463, "GTGAT":4401709, "GTGAC":3903901, "GTGAG":3058999, "GTGTA":7503454, "GTGTT":7631657, "GTGTC":6521925, "GTGTG":8744762, "GTGCA":4464373, "GTGCT":4409168, "GTGCC":3068103, "GTGCG":1149035, "GTGGA":3895763, "GTGGT":5205443, "GTGGC":2686550, "GTGGG":1980185, "GCAAA":5720603, "GCAAT":3423802, "GCAAC":4458903, "GCAAG":3149036, "GCATA":3040676, "GCATT":5349636, "GCATC":4290261, "GCATG":3824267, "GCACA":5472696, "GCACT":4548608, "GCACC":3454409, "GCACG":1199007, "GCAGA":5707952, "GCAGT":4436403, "GCAGC":6548110, "GCAGG":3631284, "GCTAA":3422781, "GCTAT":2946955, "GCTAC":3082298, "GCTAG":1496250, "GCTTA":2802400, "GCTTT":7498110, "GCTTC":5371686, "GCTTG":2758398, "GCTCA":4982905, "GCTCT":5387882, "GCTCC":4645149, "GCTCG":3404022, "GCTGA":5237441, "GCTGT":6695787, "GCTGC":6106312, "GCTGG":4011112, "GCCAA":4463626, "GCCAT":4600323, "GCCAC":3718339, "GCCAG":3675932, "GCCTA":1805277, "GCCTT":4873451, "GCCTC":4697483, "GCCTG":3293218, "GCCCA":3264871, "GCCCT":3411059, "GCCCC":3301877, "GCCCG":1366680, "GCCGA":1414626, "GCCGT":2042013, "GCCGC":1617530, "GCCGG":1266465, "GCGAA":772771, "GCGAT":922520, "GCGAC":1473133, "GCGAG":1223601, "GCGTA":933210, "GCGTT":1690526, "GCGTC":3720532, "GCGTG":1432559, "GCGCA":1160264, "GCGCT":1432706, "GCGCC":1506578, "GCGCG":607323, "GCGGA":1074884, "GCGGT":1026360, "GCGGC":1413423, "GCGGG":838565, "GGAAA":7301270, "GGAAT":2985198, "GGAAC":2715534, "GGAAG":5217678, "GGATA":2401975, "GGATT":3024580, "GGATC":2551957, "GGATG":3216351, "GGACA":4499304, "GGACT":3372805, "GGACC":2417516, "GGACG":1613151, "GGAGA":4442761, "GGAGT":2706813, "GGAGC":3095451, "GGAGG":3695837, "GGTAA":2466894, "GGTAT":2425329, "GGTAC":1702540, "GGTAG":1253711, "GGTTA":2354003, "GGTTT":4955959, "GGTTC":2740804, "GGTTG":2484886, "GGTCA":3304014, "GGTCT":2789690, "GGTCC":2127883, "GGTCG":1434193, "GGTGA":3049881, "GGTGT":4780072, "GGTGC":2904406, "GGTGG":2762585, "GGCAA":2964459, "GGCAT":2628973, "GGCAC":2228428, "GGCAG":3029789, "GGCTA":1881234, "GGCTT":3477375, "GGCTC":3169194, "GGCTG":3757597, "GGCCA":3186427, "GGCCT":2984548, "GGCCC":2122122, "GGCCG":1615877, "GGCGA":1004593, "GGCGT":1309580, "GGCGC":984372, "GGCGG":1176233, "GGGAA":4580637, "GGGAT":1985304, "GGGAC":2046192, "GGGAG":2196505, "GGGTA":1346241, "GGGTT":2083374, "GGGTC":1644993, "GGGTG":1812701, "GGGCA":1860170, "GGGCT":2248482, "GGGCC":1849259, "GGGCG":908272, "GGGGA":1651716, "GGGGT":1272120, "GGGGC":1541136, "GGGGG":1538904 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213492_sample_1703673_S126_L002_R1_001.fastq.gz --in2 lib_213492_sample_1703673_S126_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213492_sample_1703673_S126_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213492_sample_1703673_S126_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213492_sample_1703673_S126_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213492_sample_1703673_S126_L002_R2_001.fastp-trim.fq.gz " }