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"ACGTT":1355786, "ACGTC":4918518, "ACGTG":1258413, "ACGCA":826240, "ACGCT":688698, "ACGCC":420171, "ACGCG":230581, "ACGGA":1279559, "ACGGT":993327, "ACGGC":606240, "ACGGG":1100148, "AGAAA":10503333, "AGAAT":5037648, "AGAAC":3946524, "AGAAG":8291685, "AGATA":6246629, "AGATT":3396451, "AGATC":8628136, "AGATG":6769501, "AGACA":9073323, "AGACT":3255124, "AGACC":1785972, "AGACG":1573447, "AGAGA":44666943, "AGAGT":6854927, "AGAGC":9662636, "AGAGG":13801906, "AGTAA":4837196, "AGTAT":4452430, "AGTAC":2517437, "AGTAG":7942782, "AGTTA":3612983, "AGTTT":3632748, "AGTTC":1932785, "AGTTG":3419157, "AGTCA":6244233, "AGTCT":2778640, "AGTCC":1648522, "AGTCG":759101, "AGTGA":7092318, "AGTGT":7736323, "AGTGC":2094658, "AGTGG":5964306, "AGCAA":3145639, "AGCAT":3060485, "AGCAC":7059821, "AGCAG":5131550, "AGCTA":2595209, "AGCTT":2110904, "AGCTC":1725586, "AGCTG":3527207, "AGCCA":2414015, "AGCCT":2140750, "AGCCC":924578, "AGCCG":764015, "AGCGA":2025511, "AGCGT":1138052, "AGCGC":675919, "AGCGG":1034230, 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"TACCG":631353, "TACGA":762863, "TACGT":912194, "TACGC":378657, "TACGG":828895, "TAGAA":4755027, "TAGAT":5508837, "TAGAC":2520960, "TAGAG":8185618, "TAGTA":6317147, "TAGTT":3394491, "TAGTC":2262153, "TAGTG":5962574, "TAGCA":2813641, "TAGCT":2307772, "TAGCC":1638276, "TAGCG":863763, "TAGGA":3835795, "TAGGT":2971482, "TAGGC":1776137, "TAGGG":5187737, "TTAAA":5589904, "TTAAT":4447415, "TTAAC":2461478, "TTAAG":2571253, "TTATA":4070590, "TTATT":5097722, "TTATC":1806221, "TTATG":3673173, "TTACA":4228323, "TTACT":2546000, "TTACC":1371630, "TTACG":678601, "TTAGA":3890434, "TTAGT":3348634, "TTAGC":1694762, "TTAGG":3250052, "TTTAA":4879748, "TTTAT":4768285, "TTTAC":2965853, "TTTAG":3474912, "TTTTA":4988387, "TTTTT":7917438, "TTTTC":3176405, "TTTTG":4585290, "TTTCA":3938070, "TTTCT":3902730, "TTTCC":2295394, "TTTCG":744896, "TTTGA":4390737, "TTTGT":5198382, "TTTGC":2263457, "TTTGG":3951617, "TTCAA":3627120, "TTCAT":3541912, "TTCAC":2141118, "TTCAG":3393591, "TTCTA":2700785, "TTCTT":2845011, 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"CTTAG":1122862, "CTTTA":2611955, "CTTTT":2913913, "CTTTC":1905470, "CTTTG":2607469, "CTTCA":2433209, "CTTCT":2213887, "CTTCC":1357397, "CTTCG":479846, "CTTGA":2396629, "CTTGT":2505215, "CTTGC":1295143, "CTTGG":2045090, "CTCAA":2433264, "CTCAT":1973969, "CTCAC":1333712, "CTCAG":2393148, "CTCTA":1717729, "CTCTT":1843911, "CTCTC":2895701, "CTCTG":3126254, "CTCCA":4923679, "CTCCT":3827875, "CTCCC":1077136, "CTCCG":637201, "CTCGA":614098, "CTCGT":853415, "CTCGC":405454, "CTCGG":1182991, "CTGAA":7003762, "CTGAT":4447126, "CTGAC":2467522, "CTGAG":3385195, "CTGTA":4618716, "CTGTT":4079841, "CTGTC":2945355, "CTGTG":5031111, "CTGCA":2972441, "CTGCT":2734138, "CTGCC":1525779, "CTGCG":683429, "CTGGA":3451726, "CTGGT":3421344, "CTGGC":1818133, "CTGGG":3540199, "CCAAA":2841759, "CCAAT":1912729, "CCAAC":1451937, "CCAAG":1425006, "CCATA":1811651, "CCATT":2296068, "CCATC":1446217, "CCATG":2290257, "CCACA":2366767, "CCACT":1650576, "CCACC":1207266, "CCACG":582918, "CCAGA":2959273, "CCAGT":4763276, "CCAGC":1503239, "CCAGG":2319007, "CCTAA":1357992, "CCTAT":1324571, "CCTAC":1118779, "CCTAG":1456815, "CCTTA":1103097, "CCTTT":1973329, "CCTTC":1386136, "CCTTG":1848699, "CCTCA":1595965, "CCTCT":1950699, "CCTCC":1470801, "CCTCG":525966, "CCTGA":3497028, "CCTGT":2763152, "CCTGC":1520672, "CCTGG":2203780, "CCCAA":1286843, "CCCAT":1265728, "CCCAC":1012447, "CCCAG":1586493, "CCCTA":827875, "CCCTT":1013646, "CCCTC":1048688, "CCCTG":1518514, "CCCCA":1012566, "CCCCT":857718, "CCCCC":676353, "CCCCG":422755, "CCCGA":434771, "CCCGT":428473, "CCCGC":292562, "CCCGG":427220, "CCGAA":670103, "CCGAT":669255, "CCGAC":471422, "CCGAG":724091, "CCGTA":602423, "CCGTT":746647, "CCGTC":608068, "CCGTG":872271, "CCGCA":418671, "CCGCT":449205, "CCGCC":406910, "CCGCG":178518, "CCGGA":511175, "CCGGT":753963, "CCGGC":408645, "CCGGG":723536, "CGAAA":928248, "CGAAT":735610, "CGAAC":489341, "CGAAG":836540, "CGATA":941542, "CGATT":904469, "CGATC":1849446, "CGATG":1217455, "CGACA":1006718, "CGACT":587805, "CGACC":385023, "CGACG":282359, "CGAGA":2238343, "CGAGT":844109, "CGAGC":616366, "CGAGG":1191627, "CGTAA":860482, "CGTAT":735769, "CGTAC":563173, "CGTAG":932920, "CGTTA":845170, "CGTTT":1284313, "CGTTC":732978, "CGTTG":1121768, "CGTCA":961638, "CGTCT":4846273, "CGTCC":504152, "CGTCG":361383, "CGTGA":1054280, "CGTGT":1588719, "CGTGC":610202, "CGTGG":1170763, "CGCAA":614482, "CGCAT":518684, "CGCAC":535387, "CGCAG":743356, "CGCTA":492082, "CGCTT":438316, "CGCTC":501227, "CGCTG":885407, "CGCCA":535023, "CGCCT":558303, "CGCCC":290530, "CGCCG":306654, "CGCGA":238396, "CGCGT":288575, "CGCGC":223212, "CGCGG":464621, "CGGAA":7115167, "CGGAT":775160, "CGGAC":548534, "CGGAG":1380179, "CGGTA":929214, "CGGTT":1211130, "CGGTC":573991, "CGGTG":1161003, "CGGCA":808816, "CGGCT":631642, "CGGCC":465461, "CGGCG":433253, "CGGGA":968184, "CGGGT":1088408, "CGGGC":514104, "CGGGG":1639312, "GAAAA":6806958, "GAAAT":4599989, "GAAAC":3673336, "GAAAG":8292994, "GAATA":4770397, "GAATT":2550904, "GAATC":1785198, 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"GGGAC":2985392, "GGGAG":9668117, "GGGTA":4203715, "GGGTT":5416586, "GGGTC":2445979, "GGGTG":5639526, "GGGCA":2479885, "GGGCT":2703112, "GGGCC":1314266, "GGGCG":1224308, "GGGGA":6946391, "GGGGT":6359007, "GGGGC":2413248, "GGGGG":20610361 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 32859708, "total_bases": 3318830508, "q20_bases": 3151422504, "q30_bases": 2963757132, "total_cycles": 101, "quality_curves": { 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"T":[35.5838,35.8629,35.7317,35.7693,35.808,35.7407,35.789,35.7939,35.7625,35.7827,35.6741,35.7198,35.7579,35.7301,35.7404,35.7227,35.6782,35.6946,35.6683,35.6147,35.6122,35.5571,35.5439,35.5108,35.5014,35.5016,35.4697,35.4358,35.4264,35.3864,35.3588,35.3503,35.3292,35.3334,35.2783,35.2696,35.2985,35.2726,35.2277,35.1983,35.1665,35.1927,35.1732,35.1328,35.1018,35.0494,34.9587,34.9645,34.8805,34.7971,34.8504,34.7815,34.81,34.8632,34.8462,34.7947,34.7999,34.808,34.7477,34.7649,34.7611,34.8081,34.837,34.7958,34.7559,34.7151,34.6786,34.7148,34.6362,34.5888,34.5384,34.5319,34.4668,34.4214,34.4033,34.3642,34.3278,34.3277,34.3136,34.2722,34.2816,34.2063,34.2265,34.1684,34.1727,34.1425,34.1148,34.1044,34.0622,34.0412,34.1028,34.074,34.0847,34.0514,34.0159,33.9597,33.9751,33.9487,33.9531,33.929,33.503], 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"mean":[35.4097,35.8595,35.9133,35.9754,36.0017,35.9584,35.9228,35.9531,35.9478,35.9336,35.9382,35.9005,35.9047,35.8721,35.8471,35.8437,35.8159,35.8309,35.8157,35.7184,35.7313,35.673,35.6727,35.6207,35.5855,35.5644,35.5227,35.4857,35.4858,35.4459,35.4346,35.4224,35.3873,35.3888,35.3515,35.3353,35.3183,35.3035,35.2637,35.2423,35.2218,35.2183,35.2032,35.1834,35.1563,35.1421,35.1061,35.0875,35.0587,35.047,35.0306,35.0156,34.9748,34.972,34.928,34.9229,34.9086,34.9071,34.8819,34.8524,34.8235,34.8233,34.8007,34.7933,34.7402,34.7145,34.682,34.6885,34.6161,34.6072,34.571,34.5717,34.5406,34.5352,34.5031,34.4783,34.4253,34.4187,34.3982,34.3887,34.363,34.3261,34.3043,34.2661,34.2775,34.2278,34.205,34.1801,34.1334,34.1069,34.1136,34.1053,34.0763,34.0901,34.0267,34.0101,34.0135,34.0167,33.9772,33.9534,33.6691] }, "content_curves": { 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"CTTAC":2998667, "CTTAG":1496526, "CTTTA":4646727, "CTTTT":5720429, "CTTTC":7192319, "CTTTG":3776641, "CTTCA":5188007, "CTTCT":7525698, "CTTCC":5237266, "CTTCG":875370, "CTTGA":2330243, "CTTGT":3046476, "CTTGC":1919016, "CTTGG":1480988, "CTCAA":4010289, "CTCAT":5046268, "CTCAC":6960061, "CTCAG":3730456, "CTCTA":8083945, "CTCTT":8899878, "CTCTC":37213180, "CTCTG":8828651, "CTCCA":7412108, "CTCCT":10484309, "CTCCC":7906732, "CTCCG":1245679, "CTCGA":767535, "CTCGT":1253692, "CTCGC":1750535, "CTCGG":1082145, "CTGAA":3549741, "CTGAT":3302453, "CTGAC":4330036, "CTGAG":2697844, "CTGTA":6059906, "CTGTT":6648203, "CTGTC":9170582, "CTGTG":4421149, "CTGCA":3958693, "CTGCT":4892064, "CTGCC":3700151, "CTGCG":764190, "CTGGA":2728741, "CTGGT":2763724, "CTGGC":2237420, "CTGGG":1739534, "CCAAA":4050355, "CCAAT":3154568, "CCAAC":3997769, "CCAAG":2327378, "CCATA":3884918, "CCATT":4665312, "CCATC":6115135, "CCATG":3392210, "CCACA":6649548, "CCACT":5921898, "CCACC":5820034, "CCACG":1270030, "CCAGA":5195687, "CCAGT":3656903, "CCAGC":3139814, "CCAGG":2436735, "CCTAA":4294318, "CCTAT":3378477, "CCTAC":4822380, "CCTAG":2036539, "CCTTA":2996817, "CCTTT":5001006, "CCTTC":5591355, "CCTTG":2363305, "CCTCA":5415386, "CCTCT":12444610, "CCTCC":8683009, "CCTCG":1158140, "CCTGA":3133952, "CCTGT":5156235, "CCTGC":3132793, "CCTGG":2493866, "CCCAA":3534653, "CCCAT":4097659, "CCCAC":5102597, "CCCAG":4229498, "CCCTA":4877261, "CCCTT":4291486, "CCCTC":8793556, "CCCTG":4036971, "CCCCA":5408905, "CCCCT":6199431, "CCCCC":6709108, "CCCCG":1518004, "CCCGA":767030, "CCCGT":1076037, "CCCGC":1144418, "CCCGG":854661, "CCGAA":693873, "CCGAT":817463, "CCGAC":843806, "CCGAG":811430, "CCGTA":926979, "CCGTT":1058059, "CCGTC":1305161, "CCGTG":813184, "CCGCA":853158, "CCGCT":1074164, "CCGCC":1269246, "CCGCG":392361, "CCGGA":632154, "CCGGT":750752, "CCGGC":743483, "CCGGG":751612, "CGAAA":765943, "CGAAT":618150, "CGAAC":634500, "CGAAG":575608, "CGATA":746783, "CGATT":770668, "CGATC":744014, "CGATG":786901, "CGACA":1142748, "CGACT":819723, "CGACC":976216, "CGACG":384187, "CGAGA":1093556, "CGAGT":546514, "CGAGC":569081, "CGAGG":614742, "CGTAA":710563, "CGTAT":845249, "CGTAC":765785, "CGTAG":733859, "CGTTA":1057003, "CGTTT":1472326, "CGTTC":1144822, "CGTTG":982879, "CGTCA":1245428, "CGTCT":1561953, "CGTCC":2435841, "CGTCG":4551557, "CGTGA":712647, "CGTGT":4796349, "CGTGC":728011, "CGTGG":756774, "CGCAA":778861, "CGCAT":734390, "CGCAC":1055201, "CGCAG":936367, "CGCTA":917804, "CGCTT":823600, "CGCTC":2043237, "CGCTG":1200758, "CGCCA":1099413, "CGCCT":917196, "CGCCC":1335838, "CGCCG":592643, "CGCGA":227434, "CGCGT":266294, "CGCGC":510784, "CGCGG":417000, "CGGAA":6479252, "CGGAT":531392, "CGGAC":673847, "CGGAG":784661, "CGGTA":707766, "CGGTT":660382, "CGGTC":741746, "CGGTG":1112836, "CGGCA":757951, "CGGCT":765443, "CGGCC":863608, "CGGCG":466663, "CGGGA":585397, "CGGGT":491800, "CGGGC":568004, "CGGGG":1078222, "GAAAA":3443270, "GAAAT":3127408, "GAAAC":2802636, "GAAAG":4549962, "GAATA":2438719, "GAATT":2156356, "GAATC":1693509, "GAATG":2302595, "GAACA":3305193, "GAACT":1977673, "GAACC":1869097, "GAACG":840823, "GAAGA":7786366, "GAAGT":1917316, "GAAGC":1426105, "GAAGG":1673146, "GATAA":1882587, "GATAT":2297131, "GATAC":1673109, "GATAG":1306543, "GATTA":1990261, "GATTT":3009937, "GATTC":1728760, "GATTG":1833329, "GATCA":2080186, "GATCT":2616036, "GATCC":1451438, "GATCG":7071222, "GATGA":2446057, "GATGT":2714780, "GATGC":1575249, "GATGG":1666859, "GACAA":2937800, "GACAT":3370324, "GACAC":3506411, "GACAG":3300641, "GACTA":2229475, "GACTT":2128645, "GACTC":2321361, "GACTG":2725501, "GACCA":3009875, "GACCT":2112680, "GACCC":2303589, "GACCG":600409, "GACGA":889677, "GACGT":816192, "GACGC":650833, "GACGG":658177, "GAGAA":2617066, "GAGAT":3129951, "GAGAC":2280771, "GAGAG":3457732, "GAGTA":1478842, "GAGTT":1712938, "GAGTC":1424581, "GAGTG":3755445, "GAGCA":1859603, "GAGCT":1783332, "GAGCC":1562320, "GAGCG":5410245, "GAGGA":2238645, "GAGGT":1534586, "GAGGC":1317409, "GAGGG":1409947, "GTAAA":3061471, "GTAAT":2608166, "GTAAC":2184957, "GTAAG":1256640, "GTATA":3114456, "GTATT":3538103, "GTATC":2202683, "GTATG":2211137, "GTACA":3249191, "GTACT":2402518, "GTACC":2062857, "GTACG":575474, "GTAGA":2740097, "GTAGT":1987424, "GTAGC":1632123, "GTAGG":4394379, "GTTAA":2536665, "GTTAT":2925908, "GTTAC":2496366, "GTTAG":1720572, "GTTTA":3333000, "GTTTT":5230384, "GTTTC":3593707, "GTTTG":3385103, "GTTCA":3166364, "GTTCT":4011146, "GTTCC":2457586, "GTTCG":516510, "GTTGA":2538088, "GTTGT":3608669, "GTTGC":1739486, "GTTGG":1809477, "GTCAA":2603716, "GTCAT":3450223, "GTCAC":3075151, "GTCAG":2720770, "GTCTA":2642756, "GTCTT":3174117, "GTCTC":5865383, "GTCTG":4146368, "GTCCA":2860025, "GTCCT":4613471, "GTCCC":2751747, "GTCCG":655446, "GTCGA":566675, "GTCGT":6380954, "GTCGC":782650, "GTCGG":623510, "GTGAA":2337407, "GTGAT":2076358, "GTGAC":1938494, "GTGAG":1631176, "GTGTA":6940319, "GTGTT":4213193, "GTGTC":4616250, "GTGTG":6071901, "GTGCA":2114864, "GTGCT":2086085, "GTGCC":1569682, "GTGCG":620091, "GTGGA":2006944, "GTGGT":2505302, "GTGGC":1476098, "GTGGG":2529629, "GCAAA":2286951, "GCAAT":1897081, "GCAAC":1955349, "GCAAG":1475400, "GCATA":1815839, "GCATT":2651891, "GCATC":2085894, "GCATG":1958570, "GCACA":3177778, "GCACT":2211136, "GCACC":1975627, "GCACG":668762, "GCAGA":2891764, "GCAGT":2143382, "GCAGC":2393364, "GCAGG":1768470, "GCTAA":1849273, "GCTAT":2158024, "GCTAC":2482020, "GCTAG":1512223, "GCTTA":1235021, "GCTTT":3107648, "GCTTC":2290777, "GCTTG":1402008, "GCTCA":2286819, "GCTCT":4179998, "GCTCC":2377083, "GCTCG":657469, "GCTGA":2224055, "GCTGT":3502393, "GCTGC":2506642, "GCTGG":1772724, "GCCAA":2299317, "GCCAT":2750626, "GCCAC":2493038, "GCCAG":2258700, "GCCTA":1789290, "GCCTT":2344259, "GCCTC":2808680, "GCCTG":1984087, "GCCCA":2367298, "GCCCT":2417516, "GCCCC":2347512, "GCCCG":627799, "GCCGA":623131, "GCCGT":770236, "GCCGC":742297, "GCCGG":665631, "GCGAA":484575, "GCGAT":456279, "GCGAC":508281, "GCGAG":498937, "GCGTA":417930, "GCGTT":726987, 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"GGGAT":1216434, "GGGAC":1207196, "GGGAG":1457727, "GGGTA":1130608, "GGGTT":1426403, "GGGTC":990858, "GGGTG":3315691, "GGGCA":1085400, "GGGCT":1068947, "GGGCC":1151813, "GGGCG":844162, "GGGGA":1218429, "GGGGT":2223690, "GGGGC":1189307, "GGGGG":30786981 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 31622307, "total_bases": 2540403097, "q20_bases": 2471561453, "q30_bases": 2352821431, "total_cycles": 86, "quality_curves": { 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"T":[35.9376,35.9351,35.9344,35.9261,35.9111,35.9162,35.889,35.8714,35.8335,35.8186,35.7388,35.7393,35.7547,35.7476,35.719,35.7073,35.7301,35.6897,35.6975,35.6848,35.6594,35.6558,35.6364,35.6338,35.623,35.6161,35.6245,35.6051,35.5883,35.5857,35.5673,35.5473,35.5293,35.5536,35.5137,35.5176,35.5161,35.4574,35.4992,35.4524,35.5167,35.4365,35.4568,35.4291,35.4049,35.4784,35.3865,35.3678,35.425,35.3478,35.2991,35.2742,35.3088,35.2847,35.3016,35.3278,35.3336,35.314,35.2973,35.2773,35.2261,35.222,35.1993,35.1906,35.1761,35.151,35.1074,35.1593,35.161,35.1483,35.0965,35.0881,35.0705,35.0999,35.0222,35.0712,35.0716,35.072,35.0436,35.0302,34.985,35.0137,34.9681,34.9475,34.9262,34.8785], "C":[36.3906,36.3945,36.3742,36.3603,36.3581,36.3427,36.3292,36.3165,36.3033,36.2901,36.2472,36.2395,36.2308,36.2254,36.2133,36.1983,36.1873,36.1937,36.1739,36.1704,36.1584,36.1634,36.1446,36.1364,36.1249,36.1205,36.1011,36.0945,36.0579,36.0748,36.095,36.0787,36.0481,36.0625,36.059,36.0523,36.0046,36.0188,35.9837,35.9839,35.9404,35.9621,35.9671,35.953,35.9336,35.9307,35.9002,35.8993,35.8497,35.8787,35.8357,35.8501,35.8493,35.8548,35.8582,35.8544,35.827,35.8146,35.7881,35.7729,35.7408,35.7298,35.7223,35.7263,35.7271,35.6857,35.6812,35.6913,35.658,35.6536,35.6218,35.6184,35.6155,35.623,35.587,35.603,35.5953,35.5733,35.5708,35.5551,35.5229,35.5067,35.5214,35.4569,35.4847,35.3489], "G":[36.0135,36.0318,36.0154,36.0184,36.0188,36.0079,35.9927,35.9863,35.9739,35.9627,35.8873,35.8665,35.8683,35.872,35.8528,35.8437,35.8408,35.8361,35.8121,35.8079,35.7859,35.777,35.7618,35.754,35.7537,35.749,35.7053,35.7041,35.6755,35.6632,35.6716,35.6079,35.6255,35.6533,35.6085,35.6193,35.5857,35.623,35.5468,35.5384,35.5636,35.5184,35.5356,35.5317,35.4295,35.4975,35.4828,35.481,35.434,35.4614,35.3627,35.4632,35.4261,35.3775,35.3781,35.3995,35.4186,35.4122,35.3868,35.354,35.3363,35.3211,35.3325,35.3134,35.2914,35.2461,35.2143,35.2469,35.2178,35.1736,35.1393,35.1061,35.1238,35.1408,35.0916,35.0746,35.1159,35.0991,35.0795,35.0311,35.0138,34.9938,34.9923,34.9496,34.9531,34.8483], 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"CCTTG":1572776, "CCTCA":1410058, "CCTCT":1684626, "CCTCC":1297771, "CCTCG":457882, "CCTGA":1595799, "CCTGT":2329324, "CCTGC":1323697, "CCTGG":1876907, "CCCAA":1132343, "CCCAT":1125339, "CCCAC":896964, "CCCAG":1319363, "CCCTA":716241, "CCCTT":901390, "CCCTC":926496, "CCCTG":1326921, "CCCCA":882165, "CCCCT":765699, "CCCCC":556247, "CCCCG":373192, "CCCGA":363721, "CCCGT":369339, "CCCGC":248331, "CCCGG":369828, "CCGAA":543361, "CCGAT":579762, "CCGAC":410634, "CCGAG":528376, "CCGTA":491006, "CCGTT":639756, "CCGTC":508777, "CCGTG":692449, "CCGCA":346179, "CCGCT":379692, "CCGCC":356312, "CCGCG":145702, "CCGGA":392631, "CCGGT":616493, "CCGGC":322896, "CCGGG":560321, "CGAAA":728728, "CGAAT":573464, "CGAAC":395633, "CGAAG":656806, "CGATA":753030, "CGATT":735943, "CGATC":549684, "CGATG":1005188, "CGACA":781945, "CGACT":464215, "CGACC":325454, "CGACG":229514, "CGAGA":1414240, "CGAGT":663987, "CGAGC":492338, "CGAGG":943433, "CGTAA":695941, "CGTAT":578911, "CGTAC":458046, "CGTAG":707712, "CGTTA":681649, "CGTTT":1005016, "CGTTC":624298, "CGTTG":915195, "CGTCA":789203, "CGTCT":740457, "CGTCC":432985, "CGTCG":278352, "CGTGA":840021, "CGTGT":1193848, "CGTGC":506766, "CGTGG":860882, "CGCAA":504010, "CGCAT":428070, "CGCAC":450924, "CGCAG":588654, "CGCTA":401459, "CGCTT":364657, "CGCTC":427419, "CGCTG":734806, "CGCCA":441223, "CGCCT":467258, "CGCCC":248628, "CGCCG":256068, "CGCGA":184436, "CGCGT":202394, "CGCGC":182774, "CGCGG":177745, "CGGAA":730829, "CGGAT":564190, "CGGAC":441517, "CGGAG":973614, "CGGTA":735965, "CGGTT":677358, "CGGTC":467664, "CGGTG":896179, "CGGCA":615123, "CGGCT":492969, "CGGCC":388175, "CGGCG":257123, "CGGGA":688621, "CGGGT":556390, "CGGGC":391820, "CGGGG":924295, "GAAAA":5171337, "GAAAT":3629908, "GAAAC":2921847, "GAAAG":6202780, "GAATA":3736185, "GAATT":1999545, "GAATC":1474585, "GAATG":3917618, "GAACA":4291433, "GAACT":1771457, "GAACC":1025753, "GAACG":929860, "GAAGA":5983489, "GAAGT":2880367, "GAAGC":1897481, "GAAGG":4646065, "GATAA":2960394, 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"GGAAG":4434075, "GGATA":3335444, "GGATT":1869306, "GGATC":1179325, "GGATG":4095696, "GGACA":3720723, "GGACT":2088776, "GGACC":1049846, "GGACG":855843, "GGAGA":9467517, "GGAGT":3398920, "GGAGC":1918990, "GGAGG":7518353, "GGTAA":2747966, "GGTAT":2586621, "GGTAC":1740572, "GGTAG":4701740, "GGTTA":3990671, "GGTTT":2867330, "GGTTC":1462138, "GGTTG":3105911, "GGTCA":2474188, "GGTCT":2057285, "GGTCC":1134577, "GGTCG":681086, "GGTGA":3715707, "GGTGT":4271490, "GGTGC":1581942, "GGTGG":4520239, "GGCAA":1781437, "GGCAT":1649834, "GGCAC":1254966, "GGCAG":2964726, "GGCTA":2084390, "GGCTT":1351057, "GGCTC":1123325, "GGCTG":2893229, "GGCCA":1621405, "GGCCT":1446549, "GGCCC":826323, "GGCCG":574889, "GGCGA":727588, "GGCGT":666476, "GGCGC":387086, "GGCGG":833710, "GGGAA":3859112, "GGGAT":3065504, "GGGAC":2247545, "GGGAG":6692732, "GGGTA":3082271, "GGGTT":4059469, "GGGTC":1899331, "GGGTG":3634789, "GGGCA":1796458, "GGGCT":2059544, "GGGCC":1070807, "GGGCG":860546, "GGGGA":4667565, "GGGGT":4136072, "GGGGC":1713990, "GGGGG":4328695 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 31622307, "total_bases": 2538165693, "q20_bases": 2454033537, "q30_bases": 2318174486, "total_cycles": 86, "quality_curves": { "A":[36.1955,36.1882,36.2104,36.2242,36.1608,36.1624,36.1306,36.1338,36.1079,36.1108,36.1182,36.0793,36.0851,36.085,36.0574,36.0526,36.0528,36.0273,36.0234,36.0086,36.0039,35.9825,35.9867,35.948,35.9443,35.9249,35.9214,35.9052,35.8919,35.8589,35.877,35.8458,35.8235,35.811,35.8118,35.7927,35.783,35.7457,35.7371,35.6938,35.6933,35.6876,35.6949,35.6688,35.6453,35.6043,35.6035,35.5795,35.587,35.5514,35.5403,35.5045,35.5208,35.4553,35.4832,35.4512,35.4663,35.4438,35.4412,35.3949,35.3849,35.3269,35.3326,35.3196,35.3341,35.3167,35.3054,35.2652,35.2424,35.258,35.2098,35.1968,35.1786,35.1512,35.1087,35.111,35.0713,35.0742,35.0501,35.0105,34.98,35.0148,35.0147,34.9438,34.9379,34.6699], 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"C":[36.3428,36.3488,36.3407,36.3381,36.2974,36.3016,36.2845,36.2724,36.2451,36.249,36.2496,36.2101,36.1752,36.1978,36.158,36.1567,36.1438,36.1228,36.1192,36.0844,36.09,36.068,36.0576,36.0524,36.046,36.0193,36.0156,35.9776,35.9612,35.9315,35.9159,35.9259,35.907,35.8792,35.8725,35.8682,35.8439,35.818,35.7985,35.754,35.7452,35.7293,35.7245,35.7073,35.6674,35.6493,35.6267,35.6152,35.6035,35.5443,35.5344,35.5224,35.5445,35.4875,35.4761,35.435,35.4131,35.4046,35.4214,35.4198,35.3915,35.3494,35.3395,35.337,35.2886,35.285,35.2013,35.2116,35.1595,35.1728,35.1012,35.119,35.0682,35.0402,35.0204,35.0502,35.027,35.0112,35.0397,34.9751,34.9366,34.9352,34.9391,34.8966,34.8711,34.5868], 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"CTTCG":670007, "CTTGA":1843028, "CTTGT":2421200, "CTTGC":1519924, "CTTGG":1153907, "CTCAA":3131755, "CTCAT":4041842, "CTCAC":5468404, "CTCAG":2745812, "CTCTA":6313294, "CTCTT":7177138, "CTCTC":29681691, "CTCTG":6993326, "CTCCA":5672345, "CTCCT":8318610, "CTCCC":6146578, "CTCCG":977120, "CTCGA":576597, "CTCGT":971637, "CTCGC":1336253, "CTCGG":522604, "CTGAA":2803462, "CTGAT":2676806, "CTGAC":3431297, "CTGAG":2036282, "CTGTA":4884153, "CTGTT":5381577, "CTGTC":7323314, "CTGTG":3501780, "CTGCA":3107937, "CTGCT":3915586, "CTGCC":2940121, "CTGCG":598155, "CTGGA":2137201, "CTGGT":2171352, "CTGGC":1748062, "CTGGG":1331572, "CCAAA":3168596, "CCAAT":2534009, "CCAAC":3111671, "CCAAG":1746018, "CCATA":3147569, "CCATT":3851926, "CCATC":4933200, "CCATG":2762265, "CCACA":5162090, "CCACT":4722431, "CCACC":4405755, "CCACG":975551, "CCAGA":2735594, "CCAGT":2915886, "CCAGC":2482860, "CCAGG":1930673, "CCTAA":2567448, "CCTAT":2739400, "CCTAC":3868073, "CCTAG":1532010, "CCTTA":2416554, "CCTTT":4110505, "CCTTC":4528948, "CCTTG":1913411, "CCTCA":4263586, "CCTCT":9926967, "CCTCC":6878102, "CCTCG":903435, "CCTGA":2483367, "CCTGT":4101925, "CCTGC":2506267, "CCTGG":1973858, "CCCAA":2663023, "CCCAT":3254685, "CCCAC":3796382, "CCCAG":2849578, "CCCTA":3911752, "CCCTT":3482978, "CCCTC":7002553, "CCCTG":3233790, "CCCCA":3854208, "CCCCT":4892354, "CCCCC":3738003, "CCCCG":974905, "CCCGA":531492, "CCCGT":835214, "CCCGC":718170, "CCCGG":550738, "CCGAA":468357, "CCGAT":641025, "CCGAC":646217, "CCGAG":543761, "CCGTA":664266, "CCGTT":847385, "CCGTC":1019130, "CCGTG":633463, "CCGCA":602000, "CCGCT":822692, "CCGCC":858436, "CCGCG":203199, "CCGGA":456499, "CCGGT":570617, "CCGGC":514331, "CCGGG":445370, "CGAAA":598917, "CGAAT":491070, "CGAAC":495795, "CGAAG":383758, "CGATA":590915, "CGATT":612027, "CGATC":575820, "CGATG":620990, "CGACA":860269, "CGACT":635186, "CGACC":736767, "CGACG":283219, "CGAGA":679770, "CGAGT":419119, "CGAGC":421922, "CGAGG":468248, "CGTAA":561276, "CGTAT":620577, "CGTAC":606685, "CGTAG":579732, "CGTTA":846044, "CGTTT":1202019, "CGTTC":919295, "CGTTG":776059, "CGTCA":954073, "CGTCT":1226377, "CGTCC":835188, "CGTCG":269056, "CGTGA":545092, "CGTGT":922891, "CGTGC":554527, "CGTGG":524432, "CGCAA":601880, "CGCAT":582041, "CGCAC":805995, "CGCAG":618556, "CGCTA":703040, "CGCTT":650376, "CGCTC":1580450, "CGCTG":945221, "CGCCA":790622, "CGCCT":708173, "CGCCC":908595, "CGCCG":275509, "CGCGA":156037, "CGCGT":192306, "CGCGC":287538, "CGCGG":168965, "CGGAA":652476, "CGGAT":407400, "CGGAC":500760, "CGGAG":568042, "CGGTA":560032, "CGGTT":518658, "CGGTC":558472, "CGGTG":581684, "CGGCA":549702, "CGGCT":591467, "CGGCC":583087, "CGGCG":247449, "CGGGA":427606, "CGGGT":313889, "CGGGC":337545, "CGGGG":365336, "GAAAA":2809491, "GAAAT":2612553, "GAAAC":2309356, "GAAAG":1685310, "GAATA":2014515, "GAATT":1804195, "GAATC":1417546, "GAATG":1904520, "GAACA":2650818, "GAACT":1616293, "GAACC":1509147, "GAACG":667017, "GAAGA":1967525, "GAAGT":1547795, "GAAGC":1167506, "GAAGG":1288576, "GATAA":1553997, "GATAT":1911954, "GATAC":1385171, "GATAG":1025188, "GATTA":1659805, "GATTT":2507409, "GATTC":1409641, "GATTG":1500976, "GATCA":1687538, "GATCT":1721402, "GATCC":1180424, "GATCG":636412, "GATGA":2019417, "GATGT":2235200, "GATGC":1297145, "GATGG":1344112, "GACAA":2375739, "GACAT":2775512, "GACAC":2833369, "GACAG":2535099, "GACTA":1823184, "GACTT":1721115, "GACTC":1857945, "GACTG":2189361, "GACCA":2395066, "GACCT":1700739, "GACCC":1837128, "GACCG":473521, "GACGA":685429, "GACGT":648988, "GACGC":493114, "GACGG":509605, "GAGAA":2152320, "GAGAT":1659812, "GAGAC":1868839, "GAGAG":2651582, "GAGTA":1209092, "GAGTT":1385202, "GAGTC":1134935, "GAGTG":1386079, "GAGCA":1492640, "GAGCT":1446304, "GAGCC":1254646, "GAGCG":522405, "GAGGA":1811930, "GAGGT":1220589, "GAGGC":1062000, "GAGGG":978482, "GTAAA":2532054, "GTAAT":2180381, "GTAAC":1786299, "GTAAG":990356, "GTATA":2573657, "GTATT":2973894, "GTATC":1759522, "GTATG":1789523, "GTACA":2649608, "GTACT":1977299, "GTACC":1672205, "GTACG":467062, "GTAGA":1635790, "GTAGT":1595303, "GTAGC":1337043, "GTAGG":999182, "GTTAA":2067656, "GTTAT":2421163, "GTTAC":2034287, "GTTAG":1359556, "GTTTA":2683379, "GTTTT":4187198, "GTTTC":2884624, "GTTTG":2640568, "GTTCA":2504798, "GTTCT":3232428, "GTTCC":1973660, "GTTCG":399261, "GTTGA":2007488, "GTTGT":2817670, "GTTGC":1389565, "GTTGG":1254810, "GTCAA":2064210, "GTCAT":2794536, "GTCAC":2447066, "GTCAG":2109238, "GTCTA":2099220, "GTCTT":2550161, "GTCTC":4691352, "GTCTG":3311723, "GTCCA":2229976, "GTCCT":2722408, "GTCCC":2182226, "GTCCG":438536, "GTCGA":421438, "GTCGT":626231, "GTCGC":449327, "GTCGG":388588, "GTGAA":1868101, "GTGAT":1658737, "GTGAC":1553093, "GTGAG":1217793, "GTGTA":2233793, "GTGTT":3319076, "GTGTC":2246007, "GTGTG":4412679, "GTGCA":1695056, "GTGCT":1673633, "GTGCC":1248948, "GTGCG":451175, "GTGGA":1555042, "GTGGT":1564532, "GTGGC":1146684, "GTGGG":932419, "GCAAA":1831837, "GCAAT":1543441, "GCAAC":1555822, "GCAAG":1132826, "GCATA":1471190, "GCATT":2170766, "GCATC":1684711, "GCATG":1589097, "GCACA":2489682, "GCACT":1746848, "GCACC":1573422, "GCACG":518227, "GCAGA":1883648, "GCAGT":1696757, "GCAGC":1914877, "GCAGG":1379507, "GCTAA":1458510, "GCTAT":1753755, "GCTAC":1981284, "GCTAG":1163592, "GCTTA":997038, "GCTTT":2535164, "GCTTC":1835230, "GCTTG":1123764, "GCTCA":1809872, "GCTCT":3319954, "GCTCC":1887710, "GCTCG":485893, "GCTGA":1767954, "GCTGT":2790290, "GCTGC":1999223, "GCTGG":1371530, "GCCAA":1786912, "GCCAT":2213804, "GCCAC":1946035, "GCCAG":1560785, "GCCTA":1433970, "GCCTT":1894459, "GCCTC":2213629, "GCCTG":1568711, "GCCCA":1775921, "GCCCT":1915492, "GCCCC":1671222, "GCCCG":417415, "GCCGA":462375, "GCCGT":505998, "GCCGC":511459, "GCCGG":406115, "GCGAA":376945, "GCGAT":358901, "GCGAC":385625, "GCGAG":349669, "GCGTA":325620, "GCGTT":577105, "GCGTC":488821, "GCGTG":501652, "GCGCA":453973, "GCGCT":572989, "GCGCC":416860, "GCGCG":197961, "GCGGA":296496, "GCGGT":387011, "GCGGC":360777, "GCGGG":270554, "GGAAA":2023068, "GGAAT":1475320, "GGAAC":1309066, "GGAAG":1349308, "GGATA":1064095, "GGATT":1375175, "GGATC":1029622, "GGATG":1274486, "GGACA":2029801, "GGACT":1394046, "GGACC":1138242, "GGACG":456348, "GGAGA":1825619, "GGAGT":1165116, "GGAGC":1066587, "GGAGG":1351655, "GGTAA":1237604, "GGTAT":1319198, "GGTAC":1132155, "GGTAG":911532, "GGTTA":1257689, "GGTTT":1813573, "GGTTC":1044721, "GGTTG":1167899, "GGTCA":1661264, "GGTCT":1528899, "GGTCC":1040180, "GGTCG":336123, "GGTGA":1238768, "GGTGT":1750065, "GGTGC":891273, "GGTGG":1077778, "GGCAA":1141879, "GGCAT":1251749, "GGCAC":1097534, "GGCAG":1332594, "GGCTA":1375396, "GGCTT":1238868, "GGCTC":1250107, "GGCTG":1533062, "GGCCA":1583017, "GGCCT":1446640, "GGCCC":1056480, "GGCCG":413769, "GGCGA":375020, "GGCGT":350450, "GGCGC":356420, "GGCGG":380276, "GGGAA":1163218, "GGGAT":949135, "GGGAC":923215, "GGGAG":996763, "GGGTA":834683, "GGGTT":959409, "GGGTC":741621, "GGGTG":907549, "GGGCA":825460, "GGGCT":816666, "GGGCC":819608, "GGGCG":273321, "GGGGA":818884, "GGGGT":726542, "GGGGC":555946, "GGGGG":811197 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213487_sample_1703753_S132_L002_R1_001.fastq.gz --in2 lib_213487_sample_1703753_S132_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213487_sample_1703753_S132_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213487_sample_1703753_S132_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213487_sample_1703753_S132_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213487_sample_1703753_S132_L002_R2_001.fastp-trim.fq.gz " }