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"GC":[0.715424,0.553542,0.45936,0.456336,0.414767,0.398375,0.325919,0.408502,0.447466,0.334021,0.486508,0.468763,0.417277,0.411673,0.407385,0.411969,0.433935,0.413849,0.426218,0.423746,0.428241,0.42271,0.433534,0.419621,0.422457,0.432639,0.425378,0.415645,0.440718,0.415677,0.424714,0.425542,0.42828,0.424796,0.437356,0.418536,0.422023,0.431745,0.423545,0.419201,0.439221,0.421637,0.429456,0.429307,0.424391,0.424892,0.436937,0.421254,0.430854,0.429038,0.424328,0.413787,0.438966,0.424924,0.429163,0.432707,0.425188,0.423142,0.437789,0.419544,0.430412,0.43331,0.432299,0.430984,0.443573,0.422621,0.435699,0.436085,0.431285,0.425547,0.44409,0.429259,0.43335,0.442172,0.437481,0.433774,0.446284,0.430824,0.436522,0.441339,0.437161,0.432562,0.447579,0.433633,0.443818,0.446313,0.443316,0.43854,0.454979,0.437713,0.446161,0.449404,0.44606,0.442749,0.455447,0.444179,0.449453,0.450412,0.447975,0.441726,0.460474] }, "kmer_count": { "AAAAA":10988193, "AAAAT":7105219, "AAAAC":4830835, "AAAAG":5820190, 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"ACGTT":1269025, "ACGTC":4195706, "ACGTG":1190618, "ACGCA":755254, "ACGCT":1656534, "ACGCC":376443, "ACGCG":209420, "ACGGA":1110404, "ACGGT":933980, "ACGGC":545618, "ACGGG":963272, "AGAAA":8397007, "AGAAT":4382131, "AGAAC":3169063, "AGAAG":7088313, "AGATA":5509289, "AGATT":2881105, "AGATC":7715296, "AGATG":6013976, "AGACA":7563850, "AGACT":2896265, "AGACC":1829717, "AGACG":1336496, "AGAGA":32674473, "AGAGT":5446962, "AGAGC":8593091, "AGAGG":9874485, "AGTAA":4262216, "AGTAT":3881370, "AGTAC":2413686, "AGTAG":9772449, "AGTTA":3475490, "AGTTT":3447115, "AGTTC":1797555, "AGTTG":4965394, "AGTCA":5449315, "AGTCT":2555108, "AGTCC":1479464, "AGTCG":717021, "AGTGA":5968824, "AGTGT":7402741, "AGTGC":1944707, "AGTGG":5897930, "AGCAA":3170852, "AGCAT":2972882, "AGCAC":6068601, "AGCAG":6467679, "AGCTA":2480480, "AGCTT":2023054, "AGCTC":1604080, "AGCTG":5852823, "AGCCA":2183574, "AGCCT":1919313, "AGCCC":861030, "AGCCG":701488, "AGCGA":1802522, "AGCGT":1132502, "AGCGC":625363, "AGCGG":1018235, "AGGAA":5510743, "AGGAT":3701780, "AGGAC":2949076, "AGGAG":9217865, "AGGTA":4544808, "AGGTT":3863724, "AGGTC":1865071, "AGGTG":4723905, "AGGCA":2882531, "AGGCT":2456220, "AGGCC":1537774, "AGGCG":781193, "AGGGA":6494738, "AGGGT":5788963, "AGGGC":1896331, "AGGGG":6340553, "TAAAA":6469087, "TAAAT":5297947, "TAAAC":3014681, "TAAAG":4052153, "TAATA":4242801, "TAATT":3263254, "TAATC":1815783, "TAATG":5713962, "TAACA":4176738, "TAACT":2324527, "TAACC":1353920, "TAACG":893930, "TAAGA":3184438, "TAAGT":2365364, "TAAGC":1112180, "TAAGG":2945835, "TATAA":4122795, "TATAT":6302912, "TATAC":2771906, "TATAG":5411898, "TATTA":3980590, "TATTT":5499133, "TATTC":2245583, "TATTG":4177259, "TATCA":2389541, "TATCT":2564963, "TATCC":1123591, "TATCG":978624, "TATGA":3448672, "TATGT":6045476, "TATGC":1880248, "TATGG":3805561, "TACAA":3300976, "TACAT":3969147, "TACAC":2355921, "TACAG":5154417, "TACTA":1991423, "TACTT":2360848, "TACTC":1279567, "TACTG":3643562, "TACCA":1810139, "TACCT":1729963, "TACCC":807915, "TACCG":557165, "TACGA":737161, "TACGT":855614, "TACGC":356211, "TACGG":767819, "TAGAA":4328821, "TAGAT":5670517, "TAGAC":2440356, "TAGAG":6811957, "TAGTA":7547181, "TAGTT":4473461, "TAGTC":1994112, "TAGTG":6294683, "TAGCA":2627142, "TAGCT":2419172, "TAGCC":1472795, "TAGCG":885833, "TAGGA":3724398, "TAGGT":3095233, "TAGGC":1658586, "TAGGG":5314932, "TTAAA":5289842, "TTAAT":4998275, "TTAAC":2249382, "TTAAG":2441089, "TTATA":4064186, "TTATT":4899882, "TTATC":1629783, "TTATG":3703078, "TTACA":3913231, "TTACT":2406991, "TTACC":1251625, "TTACG":685960, "TTAGA":3709232, "TTAGT":3492518, "TTAGC":1596785, "TTAGG":2927685, "TTTAA":4730333, "TTTAT":4455510, "TTTAC":2758046, "TTTAG":3442317, "TTTTA":4798579, "TTTTT":7725953, "TTTTC":2962252, "TTTTG":4728486, "TTTCA":3685828, "TTTCT":3532791, "TTTCC":2240127, "TTTCG":703684, "TTTGA":4314579, "TTTGT":5352228, "TTTGC":2125153, "TTTGG":3828619, "TTCAA":3395833, "TTCAT":3153243, "TTCAC":2019967, "TTCAG":3231720, "TTCTA":2427985, "TTCTT":2802381, 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"CATCA":2908078, "CATCT":2427289, "CATCC":1389248, "CATCG":680296, "CATGA":2885494, "CATGT":4011981, "CATGC":1625545, "CATGG":2907283, "CACAA":3232786, "CACAT":2698809, "CACAC":9601297, "CACAG":3778790, "CACTA":1851529, "CACTT":1958845, "CACTC":1360907, "CACTG":2905897, "CACCA":2119703, "CACCT":1506413, "CACCC":860059, "CACCG":2255260, "CACGA":681380, "CACGT":4466403, "CACGC":521935, "CACGG":736099, "CAGAA":4324779, "CAGAT":4865347, "CAGAC":3369036, "CAGAG":7436557, "CAGTA":4760124, "CAGTT":3359402, "CAGTC":4244313, "CAGTG":5228849, "CAGCA":3529952, "CAGCT":4948850, "CAGCC":1554360, "CAGCG":1098143, "CAGGA":3682745, "CAGGT":3359847, "CAGGC":1659234, "CAGGG":3501851, "CTAAA":2714436, "CTAAT":1810223, "CTAAC":1498002, "CTAAG":1340120, "CTATA":2860169, "CTATT":2183548, "CTATC":1875107, "CTATG":2286443, "CTACA":2510295, "CTACT":1528736, "CTACC":959572, "CTACG":612594, "CTAGA":2153336, "CTAGT":2354494, "CTAGC":1206851, "CTAGG":1788077, "CTTAA":1653809, "CTTAT":1651329, "CTTAC":1036171, 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"CCAGC":1743044, "CCAGG":2134880, "CCTAA":1280443, "CCTAT":1162115, "CCTAC":1016162, "CCTAG":1269419, "CCTTA":972882, "CCTTT":1851755, "CCTTC":1314393, "CCTTG":1772198, "CCTCA":1483052, "CCTCT":1886950, "CCTCC":1357623, "CCTCG":503340, "CCTGA":1780601, "CCTGT":2739228, "CCTGC":1394201, "CCTGG":2027006, "CCCAA":1155706, "CCCAT":1205038, "CCCAC":920490, "CCCAG":1496342, "CCCTA":700590, "CCCTT":933939, "CCCTC":961243, "CCCTG":1403193, "CCCCA":923968, "CCCCT":758744, "CCCCC":627485, "CCCCG":376786, "CCCGA":397814, "CCCGT":391055, "CCCGC":271873, "CCCGG":373720, "CCGAA":648801, "CCGAT":673676, "CCGAC":425279, "CCGAG":615325, "CCGTA":556386, "CCGTT":2155101, "CCGTC":794131, "CCGTG":843905, "CCGCA":383787, "CCGCT":446546, "CCGCC":376200, "CCGCG":171436, "CCGGA":470221, "CCGGT":661606, "CCGGC":442931, "CCGGG":634350, "CGAAA":833964, "CGAAT":677566, "CGAAC":462518, "CGAAG":770440, "CGATA":896980, "CGATT":853475, "CGATC":672147, "CGATG":1200680, "CGACA":878888, "CGACT":575188, "CGACC":357214, 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"GGGAC":1115765, "GGGAG":1324782, "GGGTA":1001992, "GGGTT":1183951, "GGGTC":893893, "GGGTG":1721647, "GGGCA":1060174, "GGGCT":974401, "GGGCC":993268, "GGGCG":657703, "GGGGA":1068518, "GGGGT":1467446, "GGGGC":974892, "GGGGG":15991537 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 29798440, "total_bases": 2403880279, "q20_bases": 2334546632, "q30_bases": 2213521571, "total_cycles": 86, "quality_curves": { 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"CACAA":5712295, "CACAT":5178663, "CACAC":17382326, "CACAG":6084976, "CACTA":5132690, "CACTT":2996264, "CACTC":4593733, "CACTG":4334953, "CACCA":5119233, "CACCT":3717676, "CACCC":3116677, "CACCG":951152, "CACGA":681019, "CACGT":950018, "CACGC":1105290, "CACGG":649902, "CAGAA":2760653, "CAGAT":2963934, "CAGAC":3244687, "CAGAG":2765983, "CAGTA":3053608, "CAGTT":2870322, "CAGTC":2970410, "CAGTG":2516047, "CAGCA":3369537, "CAGCT":4847434, "CAGCC":2494441, "CAGCG":712128, "CAGGA":2140556, "CAGGT":1994164, "CAGGC":1568618, "CAGGG":1282259, "CTAAA":2792336, "CTAAT":2219133, "CTAAC":3485042, "CTAAG":912780, "CTATA":4369531, "CTATT":3157333, "CTATC":3927474, "CTATG":1938794, "CTACA":5571109, "CTACT":7577948, "CTACC":5098386, "CTACG":811500, "CTAGA":1603745, "CTAGT":1939809, "CTAGC":1562011, "CTAGG":1088053, "CTTAA":1966456, "CTTAT":2445733, "CTTAC":2115602, "CTTAG":1089003, "CTTTA":3174270, "CTTTT":4109183, "CTTTC":4494428, "CTTTG":2689552, "CTTCA":3931187, "CTTCT":5160296, "CTTCC":3455914, "CTTCG":620168, "CTTGA":2657238, "CTTGT":2255655, "CTTGC":1357247, "CTTGG":1068798, "CTCAA":3132750, "CTCAT":3553110, "CTCAC":4662987, "CTCAG":2627385, "CTCTA":5216482, "CTCTT":5310392, "CTCTC":20976687, "CTCTG":5700814, "CTCCA":5202389, "CTCCT":6620823, "CTCCC":5514864, "CTCCG":893516, "CTCGA":524619, "CTCGT":822248, "CTCGC":1192352, "CTCGG":761553, "CTGAA":2703337, "CTGAT":2481209, "CTGAC":3150370, "CTGAG":1882414, "CTGTA":4083673, "CTGTT":4462046, "CTGTC":5884961, "CTGTG":3120647, "CTGCA":3097306, "CTGCT":5056125, "CTGCC":2751977, "CTGCG":556927, "CTGGA":2408488, "CTGGT":2423441, "CTGGC":1582869, "CTGGG":1248643, "CCAAA":3084488, "CCAAT":2384114, "CCAAC":4137369, "CCAAG":1763441, "CCATA":2984626, "CCATT":3226565, "CCATC":3913366, "CCATG":2427801, "CCACA":8071807, "CCACT":4720230, "CCACC":3994503, "CCACG":977289, "CCAGA":2868257, "CCAGT":2612989, "CCAGC":2355616, "CCAGG":1797648, "CCTAA":2335577, "CCTAT":2235838, "CCTAC":4806587, "CCTAG":1519269, "CCTTA":2273736, "CCTTT":3405096, "CCTTC":3624327, "CCTTG":1732310, "CCTCA":3985819, "CCTCT":7058239, "CCTCC":5363558, "CCTCG":769050, "CCTGA":2235721, "CCTGT":3378879, "CCTGC":2370297, "CCTGG":1817861, "CCCAA":3043048, "CCCAT":2685923, "CCCAC":4222441, "CCCAG":2665773, "CCCTA":4111395, "CCCTT":2842181, "CCCTC":5115234, "CCCTG":2740571, "CCCCA":3765506, "CCCCT":4056408, "CCCCC":3096029, "CCCCG":883093, "CCCGA":518972, "CCCGT":728381, "CCCGC":650640, "CCCGG":492126, "CCGAA":443094, "CCGAT":615110, "CCGAC":656602, "CCGAG":547546, "CCGTA":660365, "CCGTT":751112, "CCGTC":868076, "CCGTG":604969, "CCGCA":629544, "CCGCT":806864, "CCGCC":783279, "CCGCG":195197, "CCGGA":420210, "CCGGT":507101, "CCGGC":488601, "CCGGG":389981, "CGAAA":574204, "CGAAT":446989, "CGAAC":472216, "CGAAG":380451, "CGATA":559302, "CGATT":588713, "CGATC":534788, "CGATG":580799, "CGACA":1033165, "CGACT":601057, "CGACC":758369, "CGACG":309007, "CGAGA":712672, "CGAGT":405568, "CGAGC":411022, "CGAGG":458984, "CGTAA":559629, "CGTAT":633505, "CGTAC":552505, "CGTAG":526092, "CGTTA":769817, "CGTTT":1082065, "CGTTC":772029, "CGTTG":736247, "CGTCA":879599, "CGTCT":1061088, "CGTCC":730082, "CGTCG":1216630, "CGTGA":518477, "CGTGT":2420466, "CGTGC":518533, "CGTGG":551936, "CGCAA":637604, "CGCAT":533171, "CGCAC":776556, "CGCAG":603345, "CGCTA":717574, "CGCTT":603600, "CGCTC":1389635, "CGCTG":933398, "CGCCA":792427, "CGCCT":646703, "CGCCC":789892, "CGCCG":353021, "CGCGA":153371, "CGCGT":168885, "CGCGC":269949, "CGCGG":172220, "CGGAA":1615378, "CGGAT":399339, "CGGAC":469795, "CGGAG":543756, "CGGTA":517306, "CGGTT":473196, "CGGTC":505795, "CGGTG":788707, "CGGCA":556673, "CGGCT":552446, "CGGCC":551149, "CGGCG":244300, "CGGGA":399611, "CGGGT":332416, "CGGGC":299943, "CGGGG":343854, "GAAAA":2595808, "GAAAT":2525138, "GAAAC":2097666, "GAAAG":2560334, "GAATA":1859854, "GAATT":1699511, "GAATC":1224849, "GAATG":1747668, "GAACA":2592951, "GAACT":1527382, "GAACC":1399948, "GAACG":642686, "GAAGA":2886057, "GAAGT":1490214, "GAAGC":1167967, "GAAGG":1217480, "GATAA":1407789, "GATAT":1781796, "GATAC":1309295, "GATAG":927312, "GATTA":1531669, "GATTT":2333794, "GATTC":1330202, "GATTG":1388352, "GATCA":1647743, "GATCT":1953834, "GATCC":1158260, "GATCG":1608801, "GATGA":1912334, "GATGT":2062799, "GATGC":1285590, "GATGG":1277732, "GACAA":2659706, "GACAT":2562636, "GACAC":3144908, "GACAG":2461029, "GACTA":1602459, "GACTT":1634733, "GACTC":1697748, "GACTG":2033717, "GACCA":2386074, "GACCT":1571591, "GACCC":1631410, "GACCG":472468, "GACGA":706935, "GACGT":622328, "GACGC":478257, "GACGG":498525, "GAGAA":2023925, "GAGAT":1765451, "GAGAC":1883740, "GAGAG":2516789, "GAGTA":1109341, "GAGTT":1282082, "GAGTC":1055147, "GAGTG":2195632, "GAGCA":1375392, "GAGCT":1375734, "GAGCC":1157313, "GAGCG":1468075, "GAGGA":1716849, "GAGGT":1156590, "GAGGC":1930819, "GAGGG":952836, "GTAAA":2354312, "GTAAT":2055075, "GTAAC":1689019, "GTAAG":913315, "GTATA":2240701, "GTATT":2650172, "GTATC":1573475, "GTATG":1613619, "GTACA":2598828, "GTACT":1939133, "GTACC":1580250, "GTACG":439368, "GTAGA":1894685, "GTAGT":1372221, "GTAGC":1212974, "GTAGG":1829952, "GTTAA":1886837, "GTTAT":2144859, "GTTAC":1929610, "GTTAG":1259969, "GTTTA":2481363, "GTTTT":3878606, "GTTTC":2393402, "GTTTG":2400082, "GTTCA":2360660, "GTTCT":2648923, "GTTCC":1892435, "GTTCG":388143, "GTTGA":1874855, "GTTGT":2451366, "GTTGC":1565675, "GTTGG":1150994, "GTCAA":1998588, "GTCAT":2522482, "GTCAC":2123423, "GTCAG":1903804, "GTCTA":2012206, "GTCTT":3183325, "GTCTC":3968552, "GTCTG":2904509, "GTCCA":2061963, "GTCCT":2305340, "GTCCC":1789787, "GTCCG":436896, "GTCGA":409845, "GTCGT":1532495, "GTCGC":533610, "GTCGG":385976, "GTGAA":1769879, "GTGAT":1573351, "GTGAC":1469498, "GTGAG":1148210, "GTGTA":3477544, "GTGTT":2964211, "GTGTC":3008372, "GTGTG":5156887, "GTGCA":1564772, "GTGCT":1528745, "GTGCC":1144242, "GTGCG":417918, "GTGGA":1545971, "GTGGT":1566644, "GTGGC":1074699, "GTGGG":859238, "GCAAA":1748956, "GCAAT":1440492, "GCAAC":1819464, "GCAAG":1135391, "GCATA":1347190, "GCATT":1980602, "GCATC":1506608, "GCATG":1384328, "GCACA":2404295, "GCACT":1630569, "GCACC":1510999, "GCACG":473790, "GCAGA":1740608, "GCAGT":1552970, "GCAGC":2061316, "GCAGG":1253341, "GCTAA":1401097, "GCTAT":1599469, "GCTAC":2151762, "GCTAG":1061226, "GCTTA":917340, "GCTTT":2269394, "GCTTC":1812181, "GCTTG":1026380, "GCTCA":1699189, "GCTCT":3117892, "GCTCC":3304538, "GCTCG":513218, "GCTGA":1724089, "GCTGT":2627809, "GCTGC":3415922, "GCTGG":1596303, "GCCAA":1715136, "GCCAT":1977224, "GCCAC":1954362, "GCCAG":1417472, "GCCTA":1337764, "GCCTT":1730849, "GCCTC":1813952, "GCCTG":1418983, "GCCCA":1689354, "GCCCT":1584871, "GCCCC":1542206, "GCCCG":394081, "GCCGA":434366, "GCCGT":523158, "GCCGC":479012, "GCCGG":379439, "GCGAA":368805, "GCGAT":342583, "GCGAC":368969, "GCGAG":327365, "GCGTA":306012, "GCGTT":517026, "GCGTC":1414020, "GCGTG":1197814, "GCGCA":418897, "GCGCT":537715, "GCGCC":394547, "GCGCG":191633, "GCGGA":285166, "GCGGT":359271, "GCGGC":365051, "GCGGG":284129, "GGAAA":2929890, "GGAAT":1363958, "GGAAC":1307531, "GGAAG":2233700, "GGATA":1005184, "GGATT":1310624, "GGATC":967158, "GGATG":1229740, "GGACA":2283891, "GGACT":1289821, "GGACC":1071081, "GGACG":466857, "GGAGA":1768705, "GGAGT":1107292, "GGAGC":1014862, "GGAGG":1289528, "GGTAA":1141538, "GGTAT":1212432, "GGTAC":1150363, "GGTAG":869199, "GGTTA":1175453, "GGTTT":1661808, "GGTTC":998810, "GGTTG":1048527, "GGTCA":1572014, "GGTCT":1587884, "GGTCC":972860, "GGTCG":451472, "GGTGA":1191437, "GGTGT":1640836, "GGTGC":841576, "GGTGG":1193743, "GGCAA":1108618, "GGCAT":1147342, "GGCAC":1056271, "GGCAG":1275379, "GGCTA":1248275, "GGCTT":1141054, "GGCTC":1154909, "GGCTG":1386625, "GGCCA":1488886, "GGCCT":1324132, "GGCCC":968412, "GGCCG":405349, "GGCGA":361408, "GGCGT":1119169, "GGCGC":340687, "GGCGG":381425, "GGGAA":2047406, "GGGAT":856382, "GGGAC":852802, "GGGAG":927489, "GGGTA":759379, "GGGTT":857650, "GGGTC":678756, "GGGTG":832515, "GGGCA":820477, "GGGCT":751166, "GGGCC":726889, "GGGCG":249853, "GGGGA":763435, "GGGGT":645248, "GGGGC":513320, "GGGGG":818322 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213483_sample_1703707_S19_L001_R1_001.fastq.gz --in2 lib_213483_sample_1703707_S19_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213483_sample_1703707_S19_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213483_sample_1703707_S19_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213483_sample_1703707_S19_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213483_sample_1703707_S19_L001_R2_001.fastp-trim.fq.gz " }