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"CATAG":2149085, "CATTA":4131592, "CATTT":6457913, "CATTC":2578768, "CATTG":3404239, "CATCA":4382528, "CATCT":3363289, "CATCC":1906682, "CATCG":1107040, "CATGA":4197861, "CATGT":4525119, "CATGC":2287237, "CATGG":2950794, "CACAA":6224274, "CACAT":4119143, "CACAC":10468595, "CACAG":4703511, "CACTA":1807827, "CACTT":3137025, "CACTC":1748845, "CACTG":3567319, "CACCA":2970539, "CACCT":4227814, "CACCC":970109, "CACCG":1069475, "CACGA":1026575, "CACGT":5825601, "CACGC":913891, "CACGG":1117722, "CAGAA":5877413, "CAGAT":6300023, "CAGAC":3570238, "CAGAG":6660615, "CAGTA":3678144, "CAGTT":4084011, "CAGTC":5313247, "CAGTG":4911895, "CAGCA":5512256, "CAGCT":3974318, "CAGCC":2190897, "CAGCG":1742313, "CAGGA":4665028, "CAGGT":3496876, "CAGGC":1973502, "CAGGG":2995274, "CTAAA":3857649, "CTAAT":2253749, "CTAAC":1360165, "CTAAG":1495745, "CTATA":2198480, "CTATT":2403500, "CTATC":1037811, "CTATG":1882472, "CTACA":4584617, "CTACT":1906783, "CTACC":727508, "CTACG":679440, "CTAGA":1891113, "CTAGT":1722177, 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"CCACA":4875121, "CCACT":3810459, "CCACC":4408074, "CCACG":1635550, "CCAGA":5488951, "CCAGT":3362933, "CCAGC":3573494, "CCAGG":2459942, "CCTAA":2727901, "CCTAT":1683071, "CCTAC":2616002, "CCTAG":1031592, "CCTTA":2790908, "CCTTT":5528647, "CCTTC":5133748, "CCTTG":2615355, "CCTCA":5523996, "CCTCT":6737736, "CCTCC":6741608, "CCTCG":1613323, "CCTGA":3752929, "CCTGT":3948082, "CCTGC":3413890, "CCTGG":2584040, "CCCAA":3314266, "CCCAT":2781360, "CCCAC":3418374, "CCCAG":3556937, "CCCTA":2169993, "CCCTT":3551616, "CCCTC":5031316, "CCCTG":2975254, "CCCCA":3907038, "CCCCT":3924435, "CCCCC":5657181, "CCCCG":1680968, "CCCGA":1152312, "CCCGT":1137063, "CCCGC":1244141, "CCCGG":1178170, "CCGAA":886821, "CCGAT":917086, "CCGAC":1357839, "CCGAG":1250709, "CCGTA":921828, "CCGTT":1315041, "CCGTC":1893314, "CCGTG":1249388, "CCGCA":1262003, "CCGCT":1393089, "CCGCC":1625411, "CCGCG":758041, "CCGGA":1057427, "CCGGT":849985, "CCGGC":1322189, "CCGGG":885943, "CGAAA":1171202, "CGAAT":654676, "CGAAC":749992, "CGAAG":816867, "CGATA":815224, "CGATT":889117, "CGATC":779564, "CGATG":1185818, "CGACA":1679468, "CGACT":1267953, "CGACC":1593797, "CGACG":781942, "CGAGA":1479546, "CGAGT":889921, "CGAGC":1159698, "CGAGG":956165, "CGTAA":1011122, "CGTAT":1043235, "CGTAC":1032712, "CGTAG":779037, "CGTTA":1212935, "CGTTT":2009486, "CGTTC":1245349, "CGTTG":1143774, "CGTCA":1707318, "CGTCT":2149110, "CGTCC":1537129, "CGTCG":5663729, "CGTGA":1055021, "CGTGT":5607561, "CGTGC":939942, "CGTGG":1208000, "CGCAA":1026152, "CGCAT":1077485, "CGCAC":1228379, "CGCAG":1408644, "CGCTA":873268, "CGCTT":1426245, "CGCTC":1526639, "CGCTG":1747631, "CGCCA":1620007, "CGCCT":1425001, "CGCCC":1694726, "CGCCG":1225418, "CGCGA":393609, "CGCGT":519501, "CGCGC":459918, "CGCGG":903082, "CGGAA":7114147, "CGGAT":859303, "CGGAC":1092710, "CGGAG":1422159, "CGGTA":2081812, "CGGTT":1001621, "CGGTC":796025, "CGGTG":1616691, "CGGCA":1223973, "CGGCT":1341694, "CGGCC":1420373, "CGGCG":871622, "CGGGA":913086, "CGGGT":663530, "CGGGC":726194, 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"GAGCT":2694090, "GAGCC":2017421, "GAGCG":6330695, "GAGGA":3175183, "GAGGT":1769265, "GAGGC":1925290, "GAGGG":1636331, "GTAAA":4113458, "GTAAT":3112424, "GTAAC":2260566, "GTAAG":2728559, "GTATA":2534270, "GTATT":3925591, "GTATC":2287145, "GTATG":2204988, "GTACA":3413959, "GTACT":2672578, "GTACC":1347541, "GTACG":759320, "GTAGA":2901591, "GTAGT":2042943, "GTAGC":1492351, "GTAGG":4513726, "GTTAA":3592969, "GTTAT":3233424, "GTTAC":2193328, "GTTAG":1539579, "GTTTA":4826231, "GTTTT":8485473, "GTTTC":4264270, "GTTTG":5157510, "GTTCA":3777349, "GTTCT":3465961, "GTTCC":2475267, "GTTCG":769976, "GTTGA":2920649, "GTTGT":4330420, "GTTGC":2391466, "GTTGG":2073758, "GTCAA":3124717, "GTCAT":3663717, "GTCAC":2952372, "GTCAG":3008748, "GTCTA":1841167, "GTCTT":3758445, "GTCTC":5531011, "GTCTG":3807350, "GTCCA":3140312, "GTCCT":3492633, "GTCCC":2110932, "GTCCG":970254, "GTCGA":803581, "GTCGT":5565413, "GTCGC":1493098, "GTCGG":1046007, "GTGAA":3215428, "GTGAT":2795017, "GTGAC":2537590, "GTGAG":2126917, 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"GGCCC":1504456, "GGCCG":1283463, "GGCGA":762989, "GGCGT":906583, "GGCGC":721067, "GGCGG":1016504, "GGGAA":4945532, "GGGAT":1308753, "GGGAC":1384616, "GGGAG":1562539, "GGGTA":965191, "GGGTT":1437532, "GGGTC":1078883, "GGGTG":1471665, "GGGCA":1279085, "GGGCT":1462988, "GGGCC":1401479, "GGGCG":876124, "GGGGA":1209379, "GGGGT":1196239, "GGGGC":1312849, "GGGGG":10078258 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 34195323, "total_bases": 2745746102, "q20_bases": 2665766634, "q30_bases": 2529136703, "total_cycles": 86, "quality_curves": { 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"C":[36.2836,36.2962,36.2717,36.2733,36.2584,36.2483,36.231,36.2156,36.1903,36.1819,36.1114,36.1189,36.1212,36.1185,36.0969,36.0908,36.0713,36.071,36.0829,36.0646,36.0667,36.0595,36.0457,36.0418,36.0323,36.0364,36.011,36.017,36.001,35.9891,36.0024,35.9995,35.9659,35.9795,35.9777,35.9958,35.9536,35.9687,35.9559,35.9654,35.9165,35.9326,35.932,35.9228,35.9084,35.9088,35.8975,35.8895,35.8717,35.8611,35.8522,35.8676,35.8664,35.8346,35.8472,35.8493,35.8292,35.831,35.8056,35.7891,35.7878,35.7727,35.7635,35.7624,35.7484,35.7311,35.7116,35.7258,35.7246,35.705,35.6862,35.6592,35.6582,35.65,35.6297,35.6429,35.6608,35.6417,35.6526,35.6207,35.6028,35.604,35.5928,35.5788,35.6018,35.4955], 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"mean":[36.052,36.0513,36.0408,36.036,36.0323,36.0214,36.0083,35.9897,35.9679,35.9622,35.8769,35.8712,35.8819,35.878,35.8575,35.8424,35.8423,35.8244,35.8305,35.8381,35.8147,35.8035,35.7814,35.7843,35.7957,35.7931,35.7687,35.7535,35.7432,35.7268,35.7375,35.7227,35.7154,35.7198,35.6998,35.6922,35.6723,35.6892,35.6715,35.6678,35.6631,35.633,35.6489,35.631,35.6109,35.6296,35.6021,35.5942,35.5951,35.5638,35.548,35.5766,35.5614,35.5167,35.5282,35.5442,35.5362,35.5469,35.5158,35.501,35.4787,35.4698,35.4691,35.4656,35.4505,35.431,35.395,35.4238,35.4197,35.4011,35.3547,35.3297,35.3205,35.3245,35.2819,35.3061,35.3528,35.3349,35.3329,35.3028,35.2731,35.2704,35.2372,35.2343,35.2468,35.1623] }, "content_curves": { 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"GC":[0.417067,0.420637,0.412149,0.417672,0.414772,0.416798,0.419081,0.419575,0.410025,0.417738,0.411182,0.411032,0.412014,0.414729,0.408644,0.414206,0.408792,0.409202,0.412331,0.413011,0.410071,0.414757,0.409991,0.410698,0.411332,0.413105,0.40587,0.412709,0.411182,0.411797,0.411478,0.412464,0.405077,0.41368,0.406357,0.408406,0.408218,0.410218,0.405967,0.411718,0.409018,0.407516,0.408366,0.410085,0.403354,0.4112,0.405446,0.404911,0.408597,0.409566,0.405148,0.410076,0.405294,0.405506,0.40719,0.408313,0.402908,0.409082,0.40406,0.404278,0.405378,0.40718,0.40366,0.408062,0.404139,0.405396,0.405633,0.406823,0.402057,0.408092,0.402589,0.403438,0.406168,0.407145,0.401549,0.408221,0.404633,0.406473,0.405594,0.407627,0.402915,0.407301,0.402694,0.404484,0.405918,0.40609] }, "kmer_count": { "AAAAA":13204272, "AAAAT":6647256, "AAAAC":5650049, "AAAAG":4846338, "AAATA":6521498, "AAATT":4270952, "AAATC":3831723, "AAATG":5549117, "AAACA":7749204, "AAACT":4349420, "AAACC":3862664, "AAACG":1546362, 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"AGGCA":1621553, "AGGCT":1856244, "AGGCC":1524859, "AGGCG":645649, "AGGGA":1465501, "AGGGT":1035360, "AGGGC":1062360, "AGGGG":900484, "TAAAA":6511944, "TAAAT":5563708, "TAAAC":4105863, "TAAAG":3598004, "TAATA":4434939, "TAATT":4636107, "TAATC":2908514, "TAATG":3436108, "TAACA":4382075, "TAACT":2997508, "TAACC":2111862, "TAACG":964579, "TAAGA":2241800, "TAAGT":2377008, "TAAGC":1529473, "TAAGG":1154238, "TATAA":3890553, "TATAT":5643259, "TATAC":2632437, "TATAG":1750186, "TATTA":4710367, "TATTT":8871669, "TATTC":3262285, "TATTG":3461562, "TATCA":3291373, "TATCT":3478601, "TATCC":2395916, "TATCG":807090, "TATGA":2967684, "TATGT":3614335, "TATGC":1841477, "TATGG":1521336, "TACAA":4193844, "TACAT":4672153, "TACAC":3949953, "TACAG":3289122, "TACTA":2999017, "TACTT":3727998, "TACTC":2221872, "TACTG":3091835, "TACCA":2333973, "TACCT":2253924, "TACCC":1536904, "TACCG":546509, "TACGA":812098, "TACGT":1050129, "TACGC":565672, "TACGG":628370, "TAGAA":2367566, "TAGAT":1968389, "TAGAC":1414373, 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"CATGG":2093663, "CACAA":4530442, "CACAT":5168526, "CACAC":10657754, "CACAG":4385270, "CACTA":2798490, "CACTT":4122598, "CACTC":3714496, "CACTG":4045533, "CACCA":4259083, "CACCT":3232669, "CACCC":2605764, "CACCG":1390207, "CACGA":916201, "CACGT":1145468, "CACGC":1350249, "CACGG":921403, "CAGAA":3623554, "CAGAT":3337180, "CAGAC":3545070, "CAGAG":3430011, "CAGTA":3074321, "CAGTT":3722049, "CAGTC":3067753, "CAGTG":3055678, "CAGCA":4952260, "CAGCT":3911602, "CAGCC":2931039, "CAGCG":1342846, "CAGGA":2889589, "CAGGT":2298553, "CAGGC":1796576, "CAGGG":1512530, "CTAAA":3251135, "CTAAT":2748039, "CTAAC":2172831, "CTAAG":1390150, "CTATA":2206533, "CTATT":2803080, "CTATC":1951377, "CTATG":1773311, "CTACA":3629469, "CTACT":3313004, "CTACC":1991815, "CTACG":1013326, "CTAGA":1364965, "CTAGT":1230804, "CTAGC":1029447, "CTAGG":628036, "CTTAA":3330902, "CTTAT":2953365, "CTTAC":2113453, "CTTAG":1210241, "CTTTA":4624388, "CTTTT":7983059, "CTTTC":4376981, "CTTTG":4330908, "CTTCA":5040602, "CTTCT":6482934, 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"CCTTT":4537508, "CCTTC":4199234, "CCTTG":2120788, "CCTCA":4420283, "CCTCT":5462881, "CCTCC":5439321, "CCTCG":1273129, "CCTGA":2958950, "CCTGT":3123629, "CCTGC":2730954, "CCTGG":2050229, "CCCAA":2472015, "CCCAT":2219581, "CCCAC":2517906, "CCCAG":2257030, "CCCTA":1781481, "CCCTT":2917091, "CCCTC":4073391, "CCCTG":2400746, "CCCCA":2682649, "CCCCT":3237394, "CCCCC":3576907, "CCCCG":1184394, "CCCGA":816275, "CCCGT":884676, "CCCGC":894876, "CCCGG":848303, "CCGAA":697770, "CCGAT":703561, "CCGAC":1044572, "CCGAG":845761, "CCGTA":585084, "CCGTT":1034671, "CCGTC":1494713, "CCGTG":934177, "CCGCA":933336, "CCGCT":1085518, "CCGCC":1198945, "CCGCG":533903, "CCGGA":785210, "CCGGT":656574, "CCGGC":954927, "CCGGG":618132, "CGAAA":944007, "CGAAT":520404, "CGAAC":580582, "CGAAG":581736, "CGATA":647945, "CGATT":711222, "CGATC":591329, "CGATG":949140, "CGACA":1248557, "CGACT":1001413, "CGACC":1228621, "CGACG":581802, "CGAGA":849811, "CGAGT":686218, "CGAGC":904451, "CGAGG":745564, "CGTAA":805120, "CGTAT":727444, "CGTAC":847743, "CGTAG":607557, "CGTTA":954476, "CGTTT":1596365, "CGTTC":1006066, "CGTTG":900444, "CGTCA":1343584, "CGTCT":1677283, "CGTCC":1220200, "CGTCG":749609, "CGTGA":822493, "CGTGT":1245026, "CGTGC":751308, "CGTGG":821190, "CGCAA":802213, "CGCAT":862926, "CGCAC":969547, "CGCAG":997588, "CGCTA":678352, "CGCTT":1063097, "CGCTC":1217082, "CGCTG":1378416, "CGCCA":1169968, "CGCCT":1130673, "CGCCC":1180946, "CGCCG":715858, "CGCGA":304488, "CGCGT":393155, "CGCGC":290256, "CGCGG":578268, "CGGAA":652982, "CGGAT":662051, "CGGAC":825276, "CGGAG":1042434, "CGGTA":602592, "CGGTT":791833, "CGGTC":609879, "CGGTG":883128, "CGGCA":906806, "CGGCT":999332, "CGGCC":1023715, "CGGCG":641457, "CGGGA":661187, "CGGGT":462665, "CGGGC":511322, "CGGGG":615896, "GAAAA":4820443, "GAAAT":3500148, "GAAAC":2999212, "GAAAG":2373753, "GAATA":2367149, "GAATT":2454608, "GAATC":1619373, "GAATG":2201085, "GAACA":3040473, "GAACT":1916228, "GAACC":1755529, "GAACG":830760, "GAAGA":2856935, "GAAGT":2260143, "GAAGC":1970539, "GAAGG":1616321, "GATAA":2348781, "GATAT":2465841, "GATAC":1399189, "GATAG":938513, "GATTA":2107044, "GATTT":3708424, "GATTC":1701369, "GATTG":1804188, "GATCA":2618575, "GATCT":1805969, "GATCC":1564413, "GATCG":744844, "GATGA":3032242, "GATGT":2865099, "GATGC":1846733, "GATGG":1658134, "GACAA":3123334, "GACAT":3214885, "GACAC":2610787, "GACAG":2498667, "GACTA":1467200, "GACTT":2549186, "GACTC":2029837, "GACTG":2324163, "GACCA":2502920, "GACCT":1921232, "GACCC":1525481, "GACCG":752883, "GACGA":1089930, "GACGT":1014280, "GACGC":945677, "GACGG":892848, "GAGAA":2872644, "GAGAT":2094300, "GAGAC":2103029, "GAGAG":2264497, "GAGTA":1382496, "GAGTT":2147700, "GAGTC":1529629, "GAGTG":1615275, "GAGCA":2234111, "GAGCT":2161387, "GAGCC":1624910, "GAGCG":807732, "GAGGA":2610997, "GAGGT":1423602, "GAGGC":1554201, "GAGGG":1179652, "GTAAA":3407909, "GTAAT":2600092, "GTAAC":1864915, "GTAAG":1222469, "GTATA":2067013, "GTATT":3326493, "GTATC":1768877, "GTATG":1801111, "GTACA":2793624, "GTACT":2225845, "GTACC":1090378, "GTACG":623298, "GTAGA":1588420, "GTAGT":1670798, "GTAGC":1226545, "GTAGG":820664, "GTTAA":2918291, "GTTAT":2686415, "GTTAC":1784713, "GTTAG":1203733, "GTTTA":3877063, "GTTTT":6849146, "GTTTC":3445184, "GTTTG":4101297, "GTTCA":2992190, "GTTCT":2794245, "GTTCC":2017016, "GTTCG":597984, "GTTGA":2310737, "GTTGT":3459233, "GTTGC":1913145, "GTTGG":1585592, "GTCAA":2484938, "GTCAT":2998266, "GTCAC":2332827, "GTCAG":2343251, "GTCTA":1452904, "GTCTT":3053010, "GTCTC":2874600, "GTCTG":3007135, "GTCCA":2450996, "GTCCT":2810861, "GTCCC":1689895, "GTCCG":769212, "GTCGA":593451, "GTCGT":881195, "GTCGC":905920, "GTCGG":728778, "GTGAA":2588527, "GTGAT":2295328, "GTGAC":2043972, "GTGAG":1637460, "GTGTA":2278278, "GTGTT":3993419, "GTGTC":2375368, "GTGTG":4541903, "GTGCA":2341364, "GTGCT":2311283, "GTGCC":1497860, "GTGCG":638584, "GTGGA":1995113, "GTGGT":1755311, "GTGGC":1393945, "GTGGG":1048640, "GCAAA":2984492, "GCAAT":1778065, "GCAAC":2317547, "GCAAG":1618268, "GCATA":1595574, "GCATT":2786974, "GCATC":2191044, "GCATG":1998859, "GCACA":2834096, "GCACT":2376981, "GCACC":1832659, "GCACG":628574, "GCAGA":2899254, "GCAGT":2311923, "GCAGC":3330833, "GCAGG":1904509, "GCTAA":1803862, "GCTAT":1517548, "GCTAC":1569626, "GCTAG":759738, "GCTTA":1459821, "GCTTT":3875099, "GCTTC":2775556, "GCTTG":1447635, "GCTCA":2634112, "GCTCT":2793730, "GCTCC":2329277, "GCTCG":782890, "GCTGA":2701552, "GCTGT":3509586, "GCTGC":3155369, "GCTGG":2041474, "GCCAA":2317570, "GCCAT":2413960, "GCCAC":1916915, "GCCAG":1762335, "GCCTA":940223, "GCCTT":2547789, "GCCTC":2433638, "GCCTG":1705587, "GCCCA":1673831, "GCCCT":1809084, "GCCCC":1745941, "GCCCG":749318, "GCCGA":739427, "GCCGT":1023425, "GCCGC":863926, "GCCGG":667208, "GCGAA":414372, "GCGAT":482610, "GCGAC":794406, "GCGAG":654070, "GCGTA":489493, "GCGTT":914260, "GCGTC":928636, "GCGTG":781101, "GCGCA":607337, "GCGCT":751322, "GCGCC":799991, "GCGCG":340785, "GCGGA":575447, "GCGGT":555500, "GCGGC":783589, "GCGGG":461758, "GGAAA":2762749, "GGAAT":1556454, "GGAAC":1418403, "GGAAG":1661113, "GGATA":1284299, "GGATT":1576223, "GGATC":1302540, "GGATG":1677343, "GGACA":2336029, "GGACT":1764607, "GGACC":1256353, "GGACG":856089, "GGAGA":2272007, "GGAGT":1413009, "GGAGC":1609954, "GGAGG":1904001, "GGTAA":1300189, "GGTAT":1281075, "GGTAC":902813, "GGTAG":646937, "GGTTA":1228843, "GGTTT":2547675, "GGTTC":1388600, "GGTTG":1306878, "GGTCA":1717744, "GGTCT":1455817, "GGTCC":1060146, "GGTCG":538558, "GGTGA":1608243, "GGTGT":1806131, "GGTGC":1442680, "GGTGG":1197369, "GGCAA":1555749, "GGCAT":1367353, "GGCAC":1172136, "GGCAG":1558211, "GGCTA":970591, "GGCTT":1817824, "GGCTC":1665748, "GGCTG":1964467, "GGCCA":1649044, "GGCCT":1557249, "GGCCC":1146120, "GGCCG":893789, "GGCGA":542463, "GGCGT":723544, "GGCGC":517164, "GGCGG":639491, "GGGAA":1374428, "GGGAT":1056369, "GGGAC":1070818, "GGGAG":1161182, "GGGTA":702540, "GGGTT":1089307, "GGGTC":814892, "GGGTG":893499, "GGGCA":976020, "GGGCT":1188336, "GGGCC":1010378, "GGGCG":514612, "GGGGA":868480, "GGGGT":652009, "GGGGC":861122, "GGGGG":872229 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213482_sample_1703699_S11_L001_R1_001.fastq.gz --in2 lib_213482_sample_1703699_S11_L001_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213482_sample_1703699_S11_L001.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213482_sample_1703699_S11_L001.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213482_sample_1703699_S11_L001_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213482_sample_1703699_S11_L001_R2_001.fastp-trim.fq.gz " }