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"GC":[0.680036,0.51498,0.419854,0.442763,0.400724,0.406005,0.334952,0.406661,0.466311,0.343388,0.494098,0.48396,0.423651,0.41454,0.413515,0.424934,0.435831,0.418211,0.436811,0.416595,0.437011,0.419062,0.424678,0.426837,0.42438,0.431144,0.432586,0.420319,0.437764,0.417793,0.433906,0.418436,0.433581,0.431448,0.436023,0.421273,0.435121,0.421906,0.42603,0.428428,0.43711,0.428397,0.425895,0.420768,0.422697,0.432324,0.428732,0.427523,0.43709,0.423498,0.424625,0.419619,0.436039,0.431657,0.436671,0.432543,0.431214,0.433467,0.435162,0.42459,0.438829,0.429996,0.437856,0.434807,0.438789,0.425945,0.441578,0.430214,0.431949,0.428514,0.43854,0.434736,0.441574,0.439274,0.442605,0.440455,0.445515,0.439109,0.446076,0.441191,0.445025,0.440265,0.445153,0.441094,0.446583,0.443925,0.447475,0.445269,0.452258,0.444145,0.452431,0.448026,0.450895,0.450106,0.453386,0.450488,0.454771,0.452385,0.455873,0.452214,0.461447] }, "kmer_count": { "AAAAA":15767867, "AAAAT":10358989, "AAAAC":7179106, "AAAAG":9148521, 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"ACGAG":1868076, "ACGTA":1353909, "ACGTT":1866047, "ACGTC":6683499, "ACGTG":1710623, "ACGCA":3693198, "ACGCT":948691, "ACGCC":566661, "ACGCG":311253, "ACGGA":1746089, "ACGGT":3165753, "ACGGC":807135, "ACGGG":1506800, "AGAAA":14214271, "AGAAT":7021123, "AGAAC":5508111, "AGAAG":11203225, "AGATA":8672487, "AGATT":4521078, "AGATC":11867701, "AGATG":9259507, "AGACA":12358877, "AGACT":4425024, "AGACC":2410190, "AGACG":2118407, "AGAGA":59175674, "AGAGT":9189306, "AGAGC":13167083, "AGAGG":19204868, "AGTAA":6672199, "AGTAT":6263874, "AGTAC":3467760, "AGTAG":11029638, "AGTTA":5032671, "AGTTT":5022400, "AGTTC":2714226, "AGTTG":4719817, "AGTCA":8473430, "AGTCT":3815201, "AGTCC":2205308, "AGTCG":1040147, "AGTGA":9681339, "AGTGT":10268575, "AGTGC":2879820, "AGTGG":8209989, "AGCAA":4301937, "AGCAT":4206046, "AGCAC":9603855, "AGCAG":6973477, "AGCTA":3580797, "AGCTT":2890467, "AGCTC":2344972, "AGCTG":4860182, "AGCCA":3287754, "AGCCT":2886327, "AGCCC":1254808, "AGCCG":1037025, "AGCGA":2725783, 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"CTAGA":3671459, "CTAGT":3370024, "CTAGC":3061668, "CTAGG":2033891, "CTTAA":3622437, "CTTAT":4342687, "CTTAC":3969017, "CTTAG":2047743, "CTTTA":6470258, "CTTTT":7842352, "CTTTC":9668049, "CTTTG":5197210, "CTTCA":7116445, "CTTCT":10115676, "CTTCC":7191278, "CTTCG":1176933, "CTTGA":3215250, "CTTGT":4219920, "CTTGC":2628226, "CTTGG":2032097, "CTCAA":5463677, "CTCAT":6881425, "CTCAC":9480707, "CTCAG":5072699, "CTCTA":11162818, "CTCTT":11971879, "CTCTC":49309982, "CTCTG":11789976, "CTCCA":10240235, "CTCCT":14644545, "CTCCC":10794906, "CTCCG":1704365, "CTCGA":1055691, "CTCGT":1752351, "CTCGC":2381071, "CTCGG":1462802, "CTGAA":4890376, "CTGAT":4558551, "CTGAC":5875400, "CTGAG":3677975, "CTGTA":8302917, "CTGTT":9330808, "CTGTC":12392506, "CTGTG":6002714, "CTGCA":5297964, "CTGCT":6664307, "CTGCC":4903558, "CTGCG":1040139, "CTGGA":3733539, "CTGGT":3719612, "CTGGC":3062413, "CTGGG":2341616, "CCAAA":5595615, "CCAAT":4347709, "CCAAC":5558194, "CCAAG":3175369, "CCATA":5599212, "CCATT":6386609, "CCATC":8427516, "CCATG":4630346, "CCACA":8966665, "CCACT":8170046, "CCACC":8122024, "CCACG":1739207, "CCAGA":7049267, "CCAGT":4971440, "CCAGC":4274467, "CCAGG":3314767, "CCTAA":4322648, "CCTAT":4783451, "CCTAC":6739820, "CCTAG":2759935, "CCTTA":4178657, "CCTTT":6931816, "CCTTC":7566790, "CCTTG":3245243, "CCTCA":7399173, "CCTCT":17254339, "CCTCC":11999447, "CCTCG":1589254, "CCTGA":4247893, "CCTGT":7080446, "CCTGC":4305577, "CCTGG":3388558, "CCCAA":4865042, "CCCAT":5600520, "CCCAC":7004912, "CCCAG":5793529, "CCCTA":6624621, "CCCTT":5813770, "CCCTC":12114377, "CCCTG":5523057, "CCCCA":7393330, "CCCCT":8369352, "CCCCC":9242677, "CCCCG":2084818, "CCCGA":1049703, "CCCGT":1486013, "CCCGC":1563971, "CCCGG":1167939, "CCGAA":842903, "CCGAT":1100741, "CCGAC":1157898, "CCGAG":1111963, "CCGTA":1261489, "CCGTT":1436744, "CCGTC":1825533, "CCGTG":1101100, "CCGCA":1161954, "CCGCT":1472832, "CCGCC":1730156, "CCGCG":546663, "CCGGA":854652, "CCGGT":2331004, "CCGGC":1008037, "CCGGG":1078555, "CGAAA":1050229, "CGAAT":857164, "CGAAC":863348, "CGAAG":695459, "CGATA":1015568, "CGATT":1050581, "CGATC":1005085, "CGATG":1072590, "CGACA":1567443, "CGACT":1123807, "CGACC":1313702, "CGACG":521887, "CGAGA":1497519, "CGAGT":748813, "CGAGC":774940, "CGAGG":830334, "CGTAA":967816, "CGTAT":1154954, "CGTAC":1037247, "CGTAG":978273, "CGTTA":1434347, "CGTTT":2026228, "CGTTC":1618679, "CGTTG":1326056, "CGTCA":1705544, "CGTCT":2130673, "CGTCC":1442229, "CGTCG":6222457, "CGTGA":980293, "CGTGT":6390074, "CGTGC":946169, "CGTGG":1139793, "CGCAA":1064252, "CGCAT":1008250, "CGCAC":1449354, "CGCAG":1250074, "CGCTA":1271921, "CGCTT":1122977, "CGCTC":2786470, "CGCTG":1632394, "CGCCA":1490622, "CGCCT":1257559, "CGCCC":1816617, "CGCCG":823374, "CGCGA":306561, "CGCGT":361994, "CGCGC":700493, "CGCGG":581417, "CGGAA":8922652, "CGGAT":714155, "CGGAC":918560, "CGGAG":1072713, "CGGTA":951236, "CGGTT":895897, "CGGTC":1002625, "CGGTG":2757851, "CGGCA":1018291, "CGGCT":1044456, "CGGCC":1155104, "CGGCG":640589, "CGGGA":791054, "CGGGT":691077, "CGGGC":772820, "CGGGG":1569510, "GAAAA":4753977, "GAAAT":4302424, "GAAAC":3662742, "GAAAG":6189895, "GAATA":3348510, "GAATT":2955829, "GAATC":2179978, "GAATG":3149760, "GAACA":4568306, "GAACT":2772150, "GAACC":2571121, "GAACG":1152963, "GAAGA":10703520, "GAAGT":2610413, "GAAGC":1959857, "GAAGG":2129679, "GATAA":2593296, "GATAT":3169896, "GATAC":2309700, "GATAG":1781567, "GATTA":2707950, "GATTT":4109698, "GATTC":2372434, "GATTG":2504284, "GATCA":2855700, "GATCT":3515080, "GATCC":1985231, "GATCG":9746078, "GATGA":3349879, "GATGT":3703390, "GATGC":2156511, "GATGG":2252351, "GACAA":4012606, "GACAT":4586557, "GACAC":4794500, "GACAG":4509974, "GACTA":3044284, "GACTT":2910744, "GACTC":3123760, "GACTG":3742385, "GACCA":4071692, "GACCT":2875351, "GACCC":3077521, "GACCG":823778, "GACGA":1211401, "GACGT":1111115, "GACGC":889357, "GACGG":897513, "GAGAA":3570861, "GAGAT":4317158, "GAGAC":3086686, "GAGAG":4495101, "GAGTA":2042989, "GAGTT":2336348, "GAGTC":1939815, "GAGTG":5077749, "GAGCA":2549188, "GAGCT":2428057, "GAGCC":2099109, "GAGCG":7437378, "GAGGA":3014516, "GAGGT":2078360, "GAGGC":1808079, "GAGGG":2008694, "GTAAA":4206061, "GTAAT":3574133, "GTAAC":3033893, "GTAAG":1728244, "GTATA":4243845, "GTATT":4844047, "GTATC":3037911, "GTATG":3002309, "GTACA":4451232, "GTACT":3308181, "GTACC":2802420, "GTACG":774803, "GTAGA":3741312, "GTAGT":2693851, "GTAGC":2201240, "GTAGG":5845871, "GTTAA":3548746, "GTTAT":4059511, "GTTAC":3361335, "GTTAG":2358723, "GTTTA":4669500, "GTTTT":7188869, "GTTTC":4953639, "GTTTG":4595933, "GTTCA":4319064, "GTTCT":5584823, "GTTCC":3383246, "GTTCG":703429, "GTTGA":3479329, "GTTGT":4888505, "GTTGC":2356549, "GTTGG":2453660, "GTCAA":3573183, "GTCAT":4654157, "GTCAC":4245755, "GTCAG":3734430, "GTCTA":5820582, "GTCTT":4387344, "GTCTC":7770553, "GTCTG":5635915, "GTCCA":3923114, "GTCCT":4554038, "GTCCC":3685935, "GTCCG":769954, "GTCGA":783286, "GTCGT":6475734, "GTCGC":1055996, "GTCGG":880507, "GTGAA":3186195, "GTGAT":2822548, "GTGAC":2641353, "GTGAG":2219352, "GTGTA":9339674, "GTGTT":5722942, "GTGTC":6175214, "GTGTG":9049855, "GTGCA":2900965, "GTGCT":2787561, "GTGCC":2130245, "GTGCG":832029, "GTGGA":2827848, "GTGGT":3365582, "GTGGC":1987966, "GTGGG":3312861, "GCAAA":3153871, "GCAAT":2582874, "GCAAC":2675169, "GCAAG":2012520, "GCATA":2479199, "GCATT":3639554, "GCATC":2842224, "GCATG":2684963, "GCACA":4367017, "GCACT":3045716, "GCACC":2705746, "GCACG":904468, "GCAGA":3739367, "GCAGT":2926579, "GCAGC":3243108, "GCAGG":2381003, "GCTAA":2541750, "GCTAT":2964448, "GCTAC":3366535, "GCTAG":2072961, "GCTTA":1690202, "GCTTT":4230073, "GCTTC":3098890, "GCTTG":1954182, "GCTCA":3108022, "GCTCT":5671639, "GCTCC":3224834, "GCTCG":892973, "GCTGA":3040140, "GCTGT":4767451, "GCTGC":3407309, "GCTGG":2394765, "GCCAA":3136199, "GCCAT":3703379, "GCCAC":3354484, "GCCAG":3017938, "GCCTA":2441649, "GCCTT":3240945, "GCCTC":3713509, "GCCTG":2709098, "GCCCA":3212215, "GCCCT":3279523, "GCCCC":3196709, "GCCCG":859686, "GCCGA":843825, "GCCGT":1035561, "GCCGC":998355, "GCCGG":2326094, "GCGAA":651879, "GCGAT":620619, "GCGAC":690230, "GCGAG":673634, "GCGTA":570748, "GCGTT":992790, "GCGTC":6817705, "GCGTG":940211, "GCGCA":833992, "GCGCT":1020678, "GCGCC":869126, "GCGCG":578083, "GCGGA":566411, "GCGGT":738859, "GCGGC":851495, "GCGGG":1232998, "GGAAA":6997527, "GGAAT":2487145, "GGAAC":2263869, "GGAAG":9879542, "GGATA":1829757, "GGATT":2295675, "GGATC":1719614, "GGATG":2179270, "GGACA":3520748, "GGACT":2359801, "GGACC":1935545, "GGACG":845965, "GGAGA":3378482, "GGAGT":2020662, "GGAGC":1850768, "GGAGG":2615851, "GGTAA":2166850, "GGTAT":2194494, "GGTAC":1850117, "GGTAG":1676112, "GGTTA":2111709, "GGTTT":3171826, "GGTTC":1788082, "GGTTG":2274251, "GGTCA":2819030, "GGTCT":2603948, "GGTCC":1756180, "GGTCG":938702, "GGTGA":2173772, "GGTGT":4641236, "GGTGC":1553818, "GGTGG":3838395, "GGCAA":2003595, "GGCAT":2125715, "GGCAC":1898805, "GGCAG":2469368, "GGCTA":2305594, "GGCTT":2097978, "GGCTC":2161787, "GGCTG":2654836, "GGCCA":2746126, "GGCCT":2459978, "GGCCC":1959187, "GGCCG":885211, "GGCGA":670390, "GGCGT":671438, "GGCGC":827377, "GGCGG":1349029, "GGGAA":5774432, "GGGAT":1637609, "GGGAC":1599598, "GGGAG":1996062, "GGGTA":1540773, "GGGTT":1920120, "GGGTC":1329080, "GGGTG":3303860, "GGGCA":1470442, "GGGCT":1447899, "GGGCC":1556118, "GGGCG":1174195, "GGGGA":1663615, "GGGGT":2952158, "GGGGC":1640038, "GGGGG":39904527 }, "overrepresented_sequences": { } }, "read1_after_filtering": { "total_reads": 43181435, "total_bases": 3468918806, "q20_bases": 3378852270, "q30_bases": 3220594078, "total_cycles": 86, "quality_curves": { "A":[36.461,36.4314,36.4367,36.4191,36.4177,36.4222,36.4007,36.393,36.3851,36.3702,36.3286,36.3197,36.3329,36.3273,36.3145,36.3022,36.3082,36.2969,36.3026,36.289,36.2722,36.2648,36.2594,36.2479,36.2578,36.2516,36.2482,36.2306,36.2173,36.2212,36.234,36.2279,36.2254,36.172,36.1931,36.1515,36.1603,36.1545,36.1705,36.1782,36.1226,36.1362,36.1602,36.0939,36.1003,36.0841,36.0604,36.0486,36.0762,36.0355,36.0425,36.0247,36.0109,35.9745,35.9586,35.9724,35.9691,35.966,35.9464,35.9333,35.9176,35.9008,35.9151,35.9149,35.8867,35.8852,35.8548,35.8602,35.8466,35.8316,35.8046,35.7955,35.7865,35.7836,35.7651,35.7623,35.7836,35.772,35.7549,35.7344,35.7017,35.6812,35.6661,35.6578,35.6639,35.5343], "T":[35.9618,35.9498,35.9491,35.9418,35.9343,35.9342,35.9166,35.8948,35.8588,35.8424,35.7643,35.7595,35.7653,35.7729,35.7579,35.7419,35.7579,35.7295,35.7277,35.72,35.6991,35.6892,35.6764,35.6672,35.668,35.6591,35.6666,35.6482,35.6229,35.6293,35.6128,35.5975,35.5842,35.606,35.5675,35.5674,35.5716,35.5191,35.5554,35.5141,35.5785,35.4967,35.5213,35.4913,35.4744,35.5379,35.4513,35.4344,35.4858,35.419,35.3649,35.3552,35.3905,35.3678,35.3864,35.4103,35.4104,35.3954,35.371,35.3558,35.3105,35.3101,35.2906,35.2886,35.282,35.2541,35.2075,35.2565,35.2535,35.2397,35.1954,35.1896,35.1715,35.2057,35.1428,35.1819,35.1839,35.1858,35.1574,35.1467,35.0952,35.1248,35.0858,35.065,35.0453,35.0211], "C":[36.3803,36.4,36.3796,36.3693,36.3669,36.3522,36.3382,36.3285,36.3091,36.2991,36.2596,36.2501,36.2544,36.2442,36.2239,36.2126,36.2035,36.2085,36.1941,36.1871,36.1807,36.1815,36.168,36.1544,36.1459,36.1419,36.1196,36.1104,36.0817,36.094,36.1149,36.0971,36.0748,36.0814,36.0772,36.079,36.0314,36.0434,35.9982,36.0075,35.9743,35.9869,35.9843,35.9824,35.9614,35.9561,35.9307,35.9352,35.8813,35.9106,35.8688,35.8881,35.8856,35.8991,35.901,35.8957,35.863,35.8487,35.8292,35.8114,35.7893,35.7762,35.7716,35.7793,35.7806,35.7413,35.7359,35.7416,35.7083,35.7035,35.6774,35.6747,35.6757,35.6783,35.6444,35.6604,35.6551,35.6372,35.6219,35.6072,35.5823,35.5688,35.5767,35.5324,35.5466,35.4198], "G":[35.9934,36.0329,36.0192,36.0206,36.0172,36.0048,35.996,35.9879,35.9681,35.9651,35.881,35.8624,35.8744,35.8742,35.8565,35.8428,35.8415,35.836,35.8146,35.8111,35.7906,35.7826,35.7669,35.7569,35.7575,35.7576,35.7117,35.7064,35.6835,35.6707,35.6797,35.6086,35.6291,35.6675,35.6151,35.6272,35.5901,35.6301,35.5545,35.546,35.5659,35.5236,35.539,35.5401,35.4343,35.507,35.4901,35.4956,35.4456,35.4666,35.3668,35.4723,35.4316,35.3829,35.3895,35.4085,35.4287,35.4235,35.3934,35.3604,35.3424,35.3321,35.3398,35.3245,35.2988,35.2527,35.2251,35.256,35.2212,35.1756,35.1515,35.111,35.1354,35.1487,35.1,35.0799,35.1238,35.1104,35.0909,35.0401,35.0205,35.0072,34.9939,34.9554,34.9631,34.8471], 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"CTTCT":2548825, "CTTCC":1633645, "CTTCG":501365, "CTTGA":2708105, "CTTGT":2802988, "CTTGC":1474685, "CTTGG":2278889, "CTCAA":2815520, "CTCAT":2280702, "CTCAC":1583907, "CTCAG":2757348, "CTCTA":1963042, "CTCTT":2184775, "CTCTC":3162640, "CTCTG":3635495, "CTCCA":2500365, "CTCCT":2385168, "CTCCC":1296867, "CTCCG":749875, "CTCGA":668314, "CTCGT":857521, "CTCGC":458677, "CTCGG":708131, "CTGAA":3892929, "CTGAT":3270473, "CTGAC":2862815, "CTGAG":3706850, "CTGTA":5202036, "CTGTT":4564899, "CTGTC":3383796, "CTGTG":5681772, "CTGCA":3241682, "CTGCT":3193127, "CTGCC":1791793, "CTGCG":774262, "CTGGA":3749963, "CTGGT":3783902, "CTGGC":2051841, "CTGGG":3874930, "CCAAA":3365642, "CCAAT":2236437, "CCAAC":1729886, "CCAAG":1589738, "CCATA":2113183, "CCATT":2745816, "CCATC":1727741, "CCATG":2717422, "CCACA":2758133, "CCACT":1938239, "CCACC":1435307, "CCACG":682859, "CCAGA":2741139, "CCAGT":2593338, "CCAGC":1803548, "CCAGG":2724261, "CCTAA":1585405, "CCTAT":1519716, "CCTAC":1293725, "CCTAG":1604756, "CCTTA":1288589, "CCTTT":2332454, "CCTTC":1625055, "CCTTG":2126278, "CCTCA":1939921, "CCTCT":2289168, "CCTCC":1735996, "CCTCG":611764, "CCTGA":2145922, "CCTGT":3171005, "CCTGC":1784550, "CCTGG":2552486, "CCCAA":1541793, "CCCAT":1514840, "CCCAC":1198437, "CCCAG":1776893, "CCCTA":980675, "CCCTT":1210358, "CCCTC":1252332, "CCCTG":1813285, "CCCCA":1177898, "CCCCT":1022568, "CCCCC":734601, "CCCCG":493022, "CCCGA":489293, "CCCGT":491700, "CCCGC":331528, "CCCGG":486949, "CCGAA":739162, "CCGAT":778381, "CCGAC":549848, "CCGAG":709250, "CCGTA":664817, "CCGTT":859286, "CCGTC":688852, "CCGTG":947939, "CCGCA":469184, "CCGCT":515455, "CCGCC":472611, "CCGCG":193121, "CCGGA":530865, "CCGGT":817119, "CCGGC":434724, "CCGGG":743189, "CGAAA":989450, "CGAAT":779841, "CGAAC":538744, "CGAAG":888318, "CGATA":1001944, "CGATT":1000235, "CGATC":731111, "CGATG":1351022, "CGACA":1047327, "CGACT":635668, "CGACC":438616, "CGACG":307312, "CGAGA":1908760, "CGAGT":927104, "CGAGC":667608, "CGAGG":1288725, "CGTAA":946843, "CGTAT":799651, "CGTAC":614549, "CGTAG":969083, "CGTTA":947845, "CGTTT":1376309, "CGTTC":854774, "CGTTG":1240145, "CGTCA":1063573, "CGTCT":1024730, "CGTCC":586683, "CGTCG":376144, "CGTGA":1145334, "CGTGT":1620238, "CGTGC":683386, "CGTGG":1171825, "CGCAA":688350, "CGCAT":585862, "CGCAC":634806, "CGCAG":799883, "CGCTA":540812, "CGCTT":501932, "CGCTC":577871, "CGCTG":1003468, "CGCCA":603070, "CGCCT":621677, "CGCCC":333514, "CGCCG":342972, "CGCGA":244909, "CGCGT":274221, "CGCGC":240636, "CGCGG":236941, "CGGAA":1015081, "CGGAT":772011, "CGGAC":603364, "CGGAG":1323299, "CGGTA":1016242, "CGGTT":924136, "CGGTC":638118, "CGGTG":1212946, "CGGCA":809343, "CGGCT":663627, "CGGCC":521480, "CGGCG":350323, "CGGGA":936403, "CGGGT":743130, "CGGGC":524751, "CGGGG":1243158, "GAAAA":7076221, "GAAAT":5049619, "GAAAC":4028648, "GAAAG":8329369, "GAATA":5255604, "GAATT":2756951, "GAATC":2011654, "GAATG":5493552, "GAACA":5908813, "GAACT":2441823, "GAACC":1404427, "GAACG":1303233, 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"GTATC":1851714, "GTATG":5620476, "GTACA":4412888, "GTACT":2740320, "GTACC":1480296, "GTACG":819337, "GTAGA":7371123, "GTAGT":7462609, "GTAGC":2663295, "GTAGG":5637261, "GTTAA":4952232, "GTTAT":4126429, "GTTAC":2434343, "GTTAG":4674773, "GTTTA":4252288, "GTTTT":4722456, "GTTTC":2875891, "GTTTG":4759131, "GTTCA":3093308, "GTTCT":3107951, "GTTCC":1701788, "GTTCG":642265, "GTTGA":4335619, "GTTGT":5793069, "GTTGC":2099580, "GTTGG":4166466, "GTCAA":3470604, "GTCAT":3493721, "GTCAC":2070517, "GTCAG":4620210, "GTCTA":2785899, "GTCTT":2423431, "GTCTC":2378835, "GTCTG":4549571, "GTCCA":2528000, "GTCCT":2190515, "GTCCC":1196149, "GTCCG":691124, "GTCGA":885802, "GTCGT":1008197, "GTCGC":499573, "GTCGG":872647, "GTGAA":5350567, "GTGAT":4723531, "GTGAC":3297905, "GTGAG":7521818, "GTGTA":7133338, "GTGTT":6734940, "GTGTC":3772720, "GTGTG":18669501, "GTGCA":3312755, "GTGCT":2776676, "GTGCC":1447215, "GTGCG":1064466, "GTGGA":6054441, "GTGGT":6998314, "GTGGC":2621372, "GTGGG":5295252, "GCAAA":3527526, "GCAAT":2296870, "GCAAC":1871036, "GCAAG":2169935, "GCATA":2134004, "GCATT":2987035, "GCATC":1750865, "GCATG":3008028, "GCACA":2899089, "GCACT":2136462, "GCACC":1191531, "GCACG":784538, "GCAGA":4263475, "GCAGT":3864201, "GCAGC":2718369, "GCAGG":3483179, "GCTAA":2293877, "GCTAT":2382371, "GCTAC":1842432, "GCTAG":2413788, "GCTTA":1290281, "GCTTT":2487888, "GCTTC":1544284, "GCTTG":2116576, "GCTCA":2163495, "GCTCT":2069839, "GCTCC":1319175, "GCTCG":561446, "GCTGA":3184424, "GCTGT":4445539, "GCTGC":2618663, "GCTGG":3349661, "GCCAA":1977793, "GCCAT":2277400, "GCCAC":1530790, "GCCAG":2390984, "GCCTA":1672922, "GCCTT":1779797, "GCCTC":1369530, "GCCTG":2293144, "GCCCA":1266729, "GCCCT":1177455, "GCCCC":752997, "GCCCG":423565, "GCCGA":678547, "GCCGT":830130, "GCCGC":439813, "GCCGG":714217, "GCGAA":731059, "GCGAT":1006538, "GCGAC":563080, "GCGAG":1842474, "GCGTA":707143, "GCGTT":959529, "GCGTC":627213, "GCGTG":1455923, "GCGCA":689925, "GCGCT":662224, "GCGCC":457171, "GCGCG":344838, "GCGGA":816615, "GCGGT":917959, "GCGGC":659962, "GCGGG":859573, "GGAAA":6277124, "GGAAT":3847351, "GGAAC":2707477, "GGAAG":6111152, "GGATA":4719397, "GGATT":2577268, "GGATC":1609284, "GGATG":5686588, "GGACA":5019842, "GGACT":2821864, "GGACC":1410110, "GGACG":1150664, "GGAGA":13145329, "GGAGT":4644320, "GGAGC":2590186, "GGAGG":10358255, "GGTAA":3792793, "GGTAT":3644274, "GGTAC":2354511, "GGTAG":6516060, "GGTTA":5511541, "GGTTT":3797382, "GGTTC":2008850, "GGTTG":4343006, "GGTCA":3363160, "GGTCT":2758494, "GGTCC":1536663, "GGTCG":903701, "GGTGA":5108647, "GGTGT":5892811, "GGTGC":2159717, "GGTGG":6265977, "GGCAA":2411154, "GGCAT":2243923, "GGCAC":1704836, "GGCAG":3917013, "GGCTA":2833451, "GGCTT":1857537, "GGCTC":1511529, "GGCTG":3938362, "GGCCA":2220603, "GGCCT":1952315, "GGCCC":1106901, "GGCCG":764247, "GGCGA":980057, "GGCGT":908206, "GGCGC":526437, "GGCGG":1094327, "GGGAA":5266797, "GGGAT":4246273, "GGGAC":3013171, "GGGAG":9098799, "GGGTA":4252874, "GGGTT":5539440, "GGGTC":2525591, "GGGTG":5050059, "GGGCA":2448839, "GGGCT":2792055, "GGGCC":1454997, "GGGCG":1140304, "GGGGA":6294719, "GGGGT":5590296, "GGGGC":2312097, "GGGGG":5866460 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 43181435, "total_bases": 3542940381, "q20_bases": 3416862275, "q30_bases": 3217035638, "total_cycles": 86, "quality_curves": { 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"T":[35.8404,35.8019,35.8128,35.7904,35.7401,35.7413,35.6785,35.6713,35.6503,35.6469,35.6568,35.6315,35.6105,35.6051,35.5832,35.5598,35.5726,35.5266,35.5252,35.4842,35.479,35.4904,35.4794,35.4443,35.4358,35.3828,35.3959,35.3677,35.3549,35.3122,35.326,35.2703,35.2771,35.2572,35.2483,35.2253,35.2213,35.1771,35.1901,35.1392,35.1242,35.0943,35.0926,35.063,35.0353,35.0006,35.0122,35.0145,34.9703,34.9247,34.9056,34.8947,34.9102,34.8478,34.83,34.8299,34.8339,34.7954,34.7806,34.7454,34.7229,34.6753,34.6738,34.6934,34.6894,34.7526,34.6992,34.6927,34.6407,34.6445,34.5992,34.5549,34.5401,34.481,34.4547,34.5028,34.4685,34.48,34.4384,34.382,34.3315,34.335,34.3106,34.3235,34.2996,33.8774], "C":[36.2872,36.291,36.2959,36.2914,36.2524,36.2515,36.2346,36.2239,36.1924,36.1957,36.2008,36.1619,36.1316,36.1441,36.109,36.0979,36.0876,36.0675,36.0675,36.0313,36.0388,36.0123,36.0039,35.9865,35.9821,35.965,35.9672,35.935,35.9178,35.877,35.8603,35.8597,35.8367,35.8085,35.7984,35.7984,35.7748,35.7503,35.7237,35.6838,35.6762,35.6588,35.6579,35.6412,35.6007,35.5788,35.5604,35.5491,35.5273,35.4619,35.4526,35.4409,35.4649,35.3999,35.3876,35.3427,35.3246,35.3121,35.3276,35.3174,35.2899,35.2362,35.2366,35.2321,35.1808,35.1688,35.0801,35.0912,35.0266,35.0419,34.9694,34.9858,34.9342,34.9001,34.8825,34.905,34.8791,34.8468,34.8705,34.8165,34.7857,34.7783,34.786,34.7292,34.7181,34.4454], "G":[36.0519,36.0476,36.0653,36.0582,35.9987,35.9925,35.9665,35.9615,35.9301,35.9282,35.9372,35.9059,35.8868,35.8833,35.8648,35.8466,35.8282,35.808,35.8303,35.7942,35.7751,35.7521,35.748,35.7227,35.7075,35.7204,35.6932,35.6951,35.6895,35.6548,35.6318,35.6249,35.5684,35.5528,35.5498,35.5339,35.5152,35.457,35.4674,35.4205,35.4452,35.4271,35.4153,35.4033,35.3792,35.3208,35.3525,35.3051,35.316,35.2422,35.2502,35.2117,35.1973,35.1249,35.1338,35.0702,35.1241,35.0477,35.0592,35.0047,34.959,34.9226,34.9006,34.8636,34.8639,34.7997,34.8484,34.7892,34.7783,34.7721,34.7219,34.6684,34.6483,34.5794,34.5581,34.4916,34.5847,34.4354,34.5316,34.3794,34.4192,34.3635,34.3978,34.406,34.37,34.0429], 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"CTTCA":5562840, "CTTCT":8057163, "CTTCC":5716587, "CTTCG":899934, "CTTGA":2536241, "CTTGT":3346201, "CTTGC":2073954, "CTTGG":1578254, "CTCAA":4261820, "CTCAT":5500215, "CTCAC":7424722, "CTCAG":3785122, "CTCTA":8723998, "CTCTT":9621632, "CTCTC":39113801, "CTCTG":9319571, "CTCCA":7861463, "CTCCT":11578442, "CTCCC":8360251, "CTCCG":1333131, "CTCGA":794370, "CTCGT":1355433, "CTCGC":1805794, "CTCGG":1148304, "CTGAA":3860774, "CTGAT":3689590, "CTGAC":4658975, "CTGAG":2801817, "CTGTA":6699439, "CTGTT":7554808, "CTGTC":9885135, "CTGTG":4749980, "CTGCA":4164288, "CTGCT":5322199, "CTGCC":3881170, "CTGCG":812676, "CTGGA":2928352, "CTGGT":2921161, "CTGGC":2383056, "CTGGG":1787867, "CCAAA":4360235, "CCAAT":3498464, "CCAAC":4304112, "CCAAG":2401127, "CCATA":4537840, "CCATT":5267320, "CCATC":6776580, "CCATG":3766877, "CCACA":6948979, "CCACT":6495504, "CCACC":6124571, "CCACG":1329145, "CCAGA":4069368, "CCAGT":3960375, "CCAGC":3367786, "CCAGG":2624622, "CCTAA":3418789, "CCTAT":3876673, "CCTAC":5396726, "CCTAG":2092584, "CCTTA":3360648, "CCTTT":5690321, "CCTTC":6109199, "CCTTG":2621972, "CCTCA":5828592, "CCTCT":13707581, "CCTCC":9474711, "CCTCG":1233852, "CCTGA":3368793, "CCTGT":5624724, "CCTGC":3434407, "CCTGG":2674045, "CCCAA":3654495, "CCCAT":4436605, "CCCAC":5176677, "CCCAG":4028272, "CCCTA":5314315, "CCCTT":4712332, "CCCTC":9614419, "CCCTG":4425384, "CCCCA":5272913, "CCCCT":6588990, "CCCCC":5092731, "CCCCG":1322730, "CCCGA":729926, "CCCGT":1148840, "CCCGC":962744, "CCCGG":743273, "CCGAA":641143, "CCGAT":865089, "CCGAC":882986, "CCGAG":763695, "CCGTA":983302, "CCGTT":1151064, "CCGTC":1424379, "CCGTG":856278, "CCGCA":835362, "CCGCT":1123765, "CCGCC":1155978, "CCGCG":276073, "CCGGA":633675, "CCGGT":2002315, "CCGGC":691045, "CCGGG":641216, "CGAAA":819549, "CGAAT":683454, "CGAAC":673412, "CGAAG":533371, "CGATA":805726, "CGATT":835683, "CGATC":777072, "CGATG":847071, "CGACA":1181794, "CGACT":868162, "CGACC":992601, "CGACG":384609, "CGAGA":985218, "CGAGT":575997, "CGAGC":573579, "CGAGG":628871, "CGTAA":766762, "CGTAT":912469, "CGTAC":830458, "CGTAG":778715, "CGTTA":1147044, "CGTTT":1653002, "CGTTC":1298652, "CGTTG":1046103, "CGTCA":1310518, "CGTCT":1672234, "CGTCC":1129276, "CGTCG":1838902, "CGTGA":753944, "CGTGT":2659908, "CGTGC":738981, "CGTGG":759837, "CGCAA":822419, "CGCAT":798604, "CGCAC":1103783, "CGCAG":859399, "CGCTA":974347, "CGCTT":887459, "CGCTC":2148955, "CGCTG":1279233, "CGCCA":1084364, "CGCCT":967396, "CGCCC":1220034, "CGCCG":516811, "CGCGA":210144, "CGCGT":259812, "CGCGC":384262, "CGCGG":231420, "CGGAA":2371585, "CGGAT":557228, "CGGAC":685485, "CGGAG":787121, "CGGTA":753892, "CGGTT":704120, "CGGTC":754783, "CGGTG":2313868, "CGGCA":746059, "CGGCT":803331, "CGGCC":780206, "CGGCG":338783, "CGGGA":576720, "CGGGT":444581, "CGGGC":451739, "CGGGG":543863, "GAAAA":3868072, "GAAAT":3599997, "GAAAC":3019317, "GAAAG":3753284, "GAATA":2769113, "GAATT":2471334, "GAATC":1812026, "GAATG":2606839, "GAACA":3668879, "GAACT":2267763, "GAACC":2073659, "GAACG":918806, "GAAGA":4200875, "GAAGT":2112533, "GAAGC":1599001, "GAAGG":1717520, "GATAA":2140934, "GATAT":2645736, "GATAC":1913018, "GATAG":1405073, "GATTA":2258454, "GATTT":3423263, "GATTC":1929688, "GATTG":2050210, "GATCA":2325274, "GATCT":2870055, "GATCC":1612408, "GATCG":2375949, "GATGA":2768695, "GATGT":3048290, "GATGC":1776713, "GATGG":1818218, "GACAA":3245660, "GACAT":3776139, "GACAC":3873379, "GACAG":3504500, "GACTA":2488520, "GACTT":2349875, "GACTC":2497654, "GACTG":3004690, "GACCA":3246492, "GACCT":2315813, "GACCC":2458992, "GACCG":648270, "GACGA":939660, "GACGT":884128, "GACGC":672750, "GACGG":694419, "GAGAA":2945766, "GAGAT":2543596, "GAGAC":2529877, "GAGAG":3472249, "GAGTA":1673505, "GAGTT":1892632, "GAGTC":1549523, "GAGTG":3318137, "GAGCA":2050748, "GAGCT":1967896, "GAGCC":1677528, "GAGCG":2189494, "GAGGA":2447395, "GAGGT":1653755, "GAGGC":1455865, "GAGGG":1377283, "GTAAA":3476706, "GTAAT":2992259, "GTAAC":2480980, "GTAAG":1371013, "GTATA":3531463, "GTATT":4076010, "GTATC":2477414, "GTATG":2446035, "GTACA":3639609, "GTACT":2731367, "GTACC":2273061, "GTACG":629644, "GTAGA":3013046, "GTAGT":2186461, "GTAGC":1806057, "GTAGG":2746393, "GTTAA":2892669, "GTTAT":3359681, "GTTAC":2740779, "GTTAG":1868893, "GTTTA":3762047, "GTTTT":5758880, "GTTTC":3980719, "GTTTG":3578553, "GTTCA":3421960, "GTTCT":4502486, "GTTCC":2714899, "GTTCG":544906, "GTTGA":2757037, "GTTGT":3823290, "GTTGC":1884674, "GTTGG":1716126, "GTCAA":2829186, "GTCAT":3772843, "GTCAC":3374280, "GTCAG":2909157, "GTCTA":4385244, "GTCTT":3531604, "GTCTC":6202823, "GTCTG":4492073, "GTCCA":3076483, "GTCCT":3627361, "GTCCC":2921839, "GTCCG":595732, "GTCGA":588698, "GTCGT":2285260, "GTCGC":784425, "GTCGG":551320, "GTGAA":2554082, "GTGAT":2258877, "GTGAC":2119854, "GTGAG":1670646, "GTGTA":5375272, "GTGTT":4516779, "GTGTC":4442236, "GTGTG":6801692, "GTGCA":2335082, "GTGCT":2252480, "GTGCC":1694922, "GTGCG":610353, "GTGGA":2161322, "GTGGT":2393425, "GTGGC":1548440, "GTGGG":1264042, "GCAAA":2528884, "GCAAT":2106725, "GCAAC":2132146, "GCAAG":1556468, "GCATA":2018474, "GCATT":2980286, "GCATC":2297241, "GCATG":2178262, "GCACA":3429739, "GCACT":2406073, "GCACC":2154068, "GCACG":698975, "GCAGA":2549980, "GCAGT":2318274, "GCAGC":2599759, "GCAGG":1861102, "GCTAA":2021636, "GCTAT":2407281, "GCTAC":2689851, "GCTAG":1608273, "GCTTA":1367328, "GCTTT":3453907, "GCTTC":2483096, "GCTTG":1555020, "GCTCA":2467223, "GCTCT":4499872, "GCTCC":2561037, "GCTCG":658359, "GCTGA":2422959, "GCTGT":3798897, "GCTGC":2716002, "GCTGG":1854235, "GCCAA":2440503, "GCCAT":2981216, "GCCAC":2614296, "GCCAG":2138647, "GCCTA":1960928, "GCCTT":2615224, "GCCTC":2923399, "GCCTG":2138092, "GCCCA":2427192, "GCCCT":2593802, "GCCCC":2258798, "GCCCG":563507, "GCCGA":631860, "GCCGT":777833, "GCCGC":679312, "GCCGG":1893249, "GCGAA":513393, "GCGAT":489111, "GCGAC":524411, "GCGAG":477357, "GCGTA":447131, "GCGTT":790272, "GCGTC":2138145, "GCGTG":680425, "GCGCA":627096, "GCGCT":776786, "GCGCC":572379, "GCGCG":261304, "GCGGA":404738, "GCGGT":528974, "GCGGC":487773, "GCGGG":368332, "GGAAA":4229947, "GGAAT":2009296, "GGAAC":1794628, "GGAAG":3329429, "GGATA":1471387, "GGATT":1855351, "GGATC":1384585, "GGATG":1730256, "GGACA":2761232, "GGACT":1870724, "GGACC":1546349, "GGACG":624208, "GGAGA":2522236, "GGAGT":1578880, "GGAGC":1445378, "GGAGG":1867955, "GGTAA":1737438, "GGTAT":1780182, "GGTAC":1505724, "GGTAG":1261640, "GGTTA":1696396, "GGTTT":2462827, "GGTTC":1426272, "GGTTG":1593931, "GGTCA":2245321, "GGTCT":2064485, "GGTCC":1399497, "GGTCG":664027, "GGTGA":1676932, "GGTGT":3458704, "GGTGC":1208146, "GGTGG":1784261, "GGCAA":1561436, "GGCAT":1711844, "GGCAC":1492110, "GGCAG":1826675, "GGCTA":1854254, "GGCTT":1686863, "GGCTC":1696085, "GGCTG":2064991, "GGCCA":2137516, "GGCCT":1960265, "GGCCC":1430365, "GGCCG":557287, "GGCGA":508037, "GGCGT":477216, "GGCGC":489895, "GGCGG":511966, "GGGAA":3046441, "GGGAT":1278115, "GGGAC":1230768, "GGGAG":1368283, "GGGTA":1150166, "GGGTT":1298887, "GGGTC":1001979, "GGGTG":1249160, "GGGCA":1123830, "GGGCT":1105073, "GGGCC":1097869, "GGGCG":377172, "GGGGA":1109162, "GGGGT":1011665, "GGGGC":751053, "GGGGG":1104317 }, "overrepresented_sequences": { } }, "command": "fastp --in1 lib_213479_sample_1703787_S149_L002_R1_001.fastq.gz --in2 lib_213479_sample_1703787_S149_L002_R2_001.fastq.gz --detect_adapter_for_pe --trim_poly_g --trim_poly_x --trim_front1 15 --trim_front2 15 --thread 50 --html /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213479_sample_1703787_S149_L002.fastp-trim.report.html --json /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213479_sample_1703787_S149_L002.fastp-trim.report.json --out1 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213479_sample_1703787_S149_L002_R1_001.fastp-trim.fq.gz --out2 /mmfs1/gscratch/scrubbed/samwhite/outputs/20260806-Fastp-FastQC-MultiQC-dittman-grc-rnaseq/lib_213479_sample_1703787_S149_L002_R2_001.fastp-trim.fq.gz " }