Multicore support not enabled. Proceeding with single-core trimming. Path to Cutadapt set as: '/usr/lusers/yaaminiv/.local/bin/cutadapt' (user defined) Cutadapt seems to be working fine (tested command '/usr/lusers/yaaminiv/.local/bin/cutadapt --version') Cutadapt version: 3.1 single-core operation. No quality encoding type selected. Assuming that the data provided uses Sanger encoded Phred scores (default) Output will be written into the directory: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/ Writing report to '/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_1_R1.fq.gz_trimming_report.txt' SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/data/zr3644_1_R1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All Read 1 sequences will be trimmed by 10 bp from their 5' end to avoid poor qualities or biases All Read 2 sequences will be trimmed by 10 bp from their 5' end to avoid poor qualities or biases (e.g. M-bias for BS-Seq applications) All Read 1 sequences will be trimmed by 10 bp from their 3' end to avoid poor qualities or biases All Read 2 sequences will be trimmed by 10 bp from their 3' end to avoid poor qualities or biases Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: '--outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore --threads 28' Output file(s) will be GZIP compressed Cutadapt seems to be fairly up-to-date (version 3.1). Setting -j 1 Writing final adapter and quality trimmed output to zr3644_1_R1_trimmed.fq.gz >>> Now performing quality (cutoff '-q 20') and adapter trimming in a single pass for the adapter sequence: 'AGATCGGAAGAGC' from file /gscratch/scrubbed/yaaminiv/Hawes/data/zr3644_1_R1.fq.gz <<< gzip: stdout: Disk quota exceeded [INFO ] multiqc : This is MultiQC v1.8 [INFO ] multiqc : Template : default [INFO ] multiqc : Searching : /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore [INFO ] cutadapt : Found 44 reports [INFO ] multiqc : Compressing plot data [INFO ] multiqc : Report : multiqc_report.html [INFO ] multiqc : Data : multiqc_data slurmstepd: error: acct_gather_profile/influxdb _send_data: curl_easy_perform failed to send data (discarded). Reason: Couldn't connect to server