FastQCFastQC Report
Sat 16 Jan 2021
zr3644_8_R1_val_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3644_8_R1_val_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42918508
Sequences flagged as poor quality0
Sequence length20-115
%GC18

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGCAAGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG1129150.2630916247135152Illumina PCR Primer Index 9 (96% over 28bp)
GACGGCAAGCCAGATCGCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG438890.10226124356419845RNA PCR Primer, Index 17 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)