SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_8_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_8_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1157.87 s (27 µs/read; 2.23 M reads/minute). === Summary === Total reads processed: 42,944,059 Reads with adapters: 18,771,452 (43.7%) Reads written (passing filters): 42,944,059 (100.0%) Total basepairs processed: 4,553,628,990 bp Quality-trimmed: 5,628,517 bp (0.1%) Total written (filtered): 4,526,859,091 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 18771452 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.6% C: 10.4% G: 6.6% T: 37.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 17148689 10736014.8 0 17148689 2 1061835 2684003.7 0 1061835 3 381925 671000.9 0 381925 4 175925 167750.2 0 175925 5 1790 41937.6 0 1790 6 435 10484.4 0 435 7 125 2621.1 0 125 8 31 655.3 0 31 9 202 163.8 0 14 188 10 411 41.0 1 4 407 11 72 10.2 1 1 71 12 11 2.6 1 0 11 13 1 0.6 1 0 1 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_8_R1_val_1.fq.gz ============================================= 42944059 sequences processed in total