SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_3_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_3_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1069.32 s (27 µs/read; 2.21 M reads/minute). === Summary === Total reads processed: 39,446,621 Reads with adapters: 17,287,588 (43.8%) Reads written (passing filters): 39,446,621 (100.0%) Total basepairs processed: 4,198,659,045 bp Quality-trimmed: 5,258,040 bp (0.1%) Total written (filtered): 4,173,950,912 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 17287588 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.6% C: 10.4% G: 6.5% T: 37.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 15807468 9861655.2 0 15807468 2 966064 2465413.8 0 966064 3 351561 616353.5 0 351561 4 160330 154088.4 0 160330 5 1021 38522.1 0 1021 6 389 9630.5 0 389 7 123 2407.6 0 123 8 25 601.9 0 25 9 187 150.5 0 7 180 10 342 37.6 1 6 336 11 60 9.4 1 2 58 12 13 2.4 1 0 13 13 2 0.6 1 0 2 14 1 0.6 1 0 1 17 1 0.6 1 0 1 18 1 0.6 1 0 1 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_3_R1_val_1.fq.gz ============================================= 39446621 sequences processed in total