SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_24_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_24_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1461.67 s (28 µs/read; 2.17 M reads/minute). === Summary === Total reads processed: 52,922,544 Reads with adapters: 23,174,909 (43.8%) Reads written (passing filters): 52,922,544 (100.0%) Total basepairs processed: 5,625,540,317 bp Quality-trimmed: 7,113,215 bp (0.1%) Total written (filtered): 5,592,330,989 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 23174909 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.6% C: 10.4% G: 6.5% T: 37.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 21176762 13230636.0 0 21176762 2 1303077 3307659.0 0 1303077 3 474958 826914.8 0 474958 4 217209 206728.7 0 217209 5 1352 51682.2 0 1352 6 526 12920.5 0 526 7 159 3230.1 0 159 8 46 807.5 0 46 9 242 201.9 0 22 220 10 465 50.5 1 8 457 11 98 12.6 1 0 98 12 13 3.2 1 0 13 13 1 0.8 1 0 1 15 1 0.8 1 0 1 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_24_R1_val_1.fq.gz ============================================= 52922544 sequences processed in total