SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_18_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_18_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1378.13 s (27 µs/read; 2.25 M reads/minute). === Summary === Total reads processed: 51,713,176 Reads with adapters: 22,562,188 (43.6%) Reads written (passing filters): 51,713,176 (100.0%) Total basepairs processed: 5,465,399,814 bp Quality-trimmed: 6,523,423 bp (0.1%) Total written (filtered): 5,433,434,497 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 22562188 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.6% C: 10.4% G: 6.6% T: 37.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 20591166 12928294.0 0 20591166 2 1288102 3232073.5 0 1288102 3 464974 808018.4 0 464974 4 215050 202004.6 0 215050 5 1399 50501.1 0 1399 6 504 12625.3 0 504 7 141 3156.3 0 141 8 34 789.1 0 34 9 237 197.3 0 19 218 10 446 49.3 1 6 440 11 110 12.3 1 0 110 12 16 3.1 1 0 16 13 7 0.8 1 0 7 18 1 0.8 1 0 1 20 1 0.8 1 0 1 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_18_R1_val_1.fq.gz ============================================= 51713176 sequences processed in total