SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_17_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_17_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1556.05 s (27 µs/read; 2.25 M reads/minute). === Summary === Total reads processed: 58,307,693 Reads with adapters: 25,412,767 (43.6%) Reads written (passing filters): 58,307,693 (100.0%) Total basepairs processed: 6,158,828,348 bp Quality-trimmed: 7,031,454 bp (0.1%) Total written (filtered): 6,123,151,465 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 25412767 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.6% C: 10.5% G: 6.6% T: 37.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 23203313 14576923.2 0 23203313 2 1440507 3644230.8 0 1440507 3 523661 911057.7 0 523661 4 241985 227764.4 0 241985 5 1584 56941.1 0 1584 6 542 14235.3 0 542 7 179 3558.8 0 179 8 44 889.7 0 44 9 291 222.4 0 21 270 10 544 55.6 1 6 538 11 93 13.9 1 1 92 12 17 3.5 1 0 17 13 3 0.9 1 0 3 17 1 0.9 1 0 1 19 2 0.9 1 0 2 22 1 0.9 1 0 1 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_17_R1_val_1.fq.gz ============================================= 58307693 sequences processed in total