SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_16_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_16_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1421.46 s (28 µs/read; 2.18 M reads/minute). === Summary === Total reads processed: 51,615,953 Reads with adapters: 22,358,370 (43.3%) Reads written (passing filters): 51,615,953 (100.0%) Total basepairs processed: 5,398,887,438 bp Quality-trimmed: 7,587,870 bp (0.1%) Total written (filtered): 5,366,099,765 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 22358370 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.5% C: 10.5% G: 6.7% T: 37.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 20416303 12903988.2 0 20416303 2 1263682 3225997.1 0 1263682 3 465476 806499.3 0 465476 4 209912 201624.8 0 209912 5 1454 50406.2 0 1454 6 467 12601.6 0 467 7 180 3150.4 0 180 8 44 787.6 0 44 9 278 196.9 0 35 243 10 461 49.2 1 7 454 11 96 12.3 1 0 96 12 13 3.1 1 0 13 13 4 0.8 1 0 4 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_16_R1_val_1.fq.gz ============================================= 51615953 sequences processed in total