SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_15_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_15_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1618.49 s (27 µs/read; 2.19 M reads/minute). === Summary === Total reads processed: 59,137,561 Reads with adapters: 25,905,284 (43.8%) Reads written (passing filters): 59,137,561 (100.0%) Total basepairs processed: 6,290,652,174 bp Quality-trimmed: 7,940,179 bp (0.1%) Total written (filtered): 6,253,550,020 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 25905284 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.7% C: 10.3% G: 6.5% T: 37.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 23677624 14784390.2 0 23677624 2 1453224 3696097.6 0 1453224 3 528809 924024.4 0 528809 4 242203 231006.1 0 242203 5 1675 57751.5 0 1675 6 610 14437.9 0 610 7 166 3609.5 0 166 8 55 902.4 0 55 9 287 225.6 0 18 269 10 517 56.4 1 4 513 11 94 14.1 1 0 94 12 20 3.5 1 0 20 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_15_R1_val_1.fq.gz ============================================= 59137561 sequences processed in total