SUMMARISING RUN PARAMETERS ========================== Input filename: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_14_R1_val_1.fq.gz Trimming mode: paired-end Trim Galore version: 0.6.6 Cutadapt version: 3.1 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; user defined) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Running FastQC on the data once trimming has completed Running FastQC with the following extra arguments: --outdir /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2 --threads 28 Output file will be GZIP compressed This is cutadapt 3.1 with Python 3.8.5 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_14_R1_val_1.fq.gz Processing reads on 1 core in single-end mode ... Finished in 1083.91 s (29 µs/read; 2.04 M reads/minute). === Summary === Total reads processed: 36,871,756 Reads with adapters: 16,142,874 (43.8%) Reads written (passing filters): 36,871,756 (100.0%) Total basepairs processed: 3,913,478,219 bp Quality-trimmed: 6,690,699 bp (0.2%) Total written (filtered): 3,888,601,182 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 16142874 times No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 45.2% C: 10.5% G: 6.6% T: 37.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 14740280 9217939.0 0 14740280 2 917217 2304484.8 0 917217 3 335971 576121.2 0 335971 4 146884 144030.3 0 146884 5 1359 36007.6 0 1359 6 420 9001.9 0 420 7 134 2250.5 0 134 8 35 562.6 0 35 9 199 140.7 0 16 183 10 299 35.2 1 4 295 11 64 8.8 1 1 63 12 10 2.2 1 0 10 13 1 0.5 1 0 1 24 1 0.5 1 0 1 RUN STATISTICS FOR INPUT FILE: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore/zr3644_14_R1_val_1.fq.gz ============================================= 36871756 sequences processed in total